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Citations for Millipore Sigma :
4151 - 4200 of 10000+ citations for Suppressor of CDC2 With RNA Binding Motif 2 RBMS1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2020Quote: RNA isolation from uninucleated microspores was performed by using the Spectrum™ Plant Total RNA Kit (Sigma-Aldrich) according to manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... RNA extraction was carried out with the Spectrum Plant Total RNA Mini Kit (Sigma-Aldrich, St. Louis, MO) or the RNeasy Mini Kit (Qiagen ...
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bioRxiv - Plant Biology 2020Quote: ... RNA was extracted from 4-week-old Arabidopsis leaves using the Spectrum plant total RNA kit (Sigma-Aldritch) according to manufacturer’s instructions.
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bioRxiv - Developmental Biology 2019Quote: Total RNA was extracted using the GenElute™ Mammalian Total RNA Miniprep Kit Q-PCR (Sigma, Cat#RTN350), and reverse transcription was performed using an oligoDT primer using superscript II (Thermofisher ...
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bioRxiv - Plant Biology 2020Quote: ... Total RNA was extracted from both seed and seedling samples using the Spectrum Plant RNA kit (Sigma-Aldrich), including an on-column DNase step ...
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Glutathione S-transferase: A Candidate Gene for Berry Color in Muscadine Grapes (Vitis rotundifolia)bioRxiv - Genetics 2020Quote: ... RNA was isolated from berry skins using Spectrum™ plant total RNA kit (Sigma-Aldrich, St. Louis, MO) according to the manufacturer’s instructions and from the seeds as described by Reid et al ...
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bioRxiv - Cancer Biology 2020Quote: RNA was extracted from cell lines and homogenized tumors using a GenElute Mammalian Total RNA Purification Kit (Sigma) according to standard protocols ...
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bioRxiv - Developmental Biology 2020Quote: ... DIG-labeled RNA probes were generated using the DIG RNA Labeling Kit SP6/T7 (Sigma-Aldrich cat. # 11175025910) according to manufacturer’s protocols ...
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bioRxiv - Plant Biology 2021Quote: ... and total RNA was extracted with the Spectrum™ Plant Total RNA Kit (Sigma Aldrich, Saint Louis, USA). The isolated RNA was treated with deoxyribonuclease I enzyme (Sigma Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... RNA was extracted from 100 mg of frozen tissue using the Spectrum total RNA plant kit (Sigma-Aldrich). The mRNA library was prepared by poly-A enrichment and paired-end sequencing was performed on Illumina NovaSeq6000 (2 × 150 bp ...
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bioRxiv - Genomics 2021Quote: The total RNA of each sample was isolated using the Spectrum™ Plant Total RNA kit (Sigma, USA). Seedlings (shoot and root ...
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bioRxiv - Plant Biology 2021Quote: RNA was extracted from three biological replicates per genotype using the Spectrum Plant Total RNA Kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... The crude RNA extract was further purified using the GenElute™ Mammalian Total RNA Miniprep kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: Total RNA from individual larva of Tribolium was extracted using the GenElute™ Mammalian Total RNA kit (Sigma). cDNA synthesis was carried out as previously described [72,73] ...
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bioRxiv - Systems Biology 2019Quote: Isolated RNA (200 μg for total RNA and 0.6-7.8 μg for mRNA and RNA genome) was incubated with NP1 (1 unit/μg RNA, Sigma) in a solution containing 30 mM sodium acetate pH 5.5 ...
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bioRxiv - Plant Biology 2019Quote: The total RNA was extracted using Spectrum™ Plant Total RNA kit as per manufacturer’s protocol (Sigma-Aldrich). The qRT-PCR was performed with mixture of 2 μL of primer mix (2 μM from each F & R primer) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Total RNA was extracted from the frozen leaves using the Spectrum™ Plant Total RNA Extraction Kit (Sigma). Silica-dried leaves of two species (Stewartia malacodendron and Laplacea fruticosa ...
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bioRxiv - Developmental Biology 2020Quote: Cells were lysed and RNA extracted with the GenElute™ Mammalian RNA Extraction Kit (cat. RTN70, Sigma-Aldrich) and reverse transcribed into cDNA using SuperScript® III First-Strand Synthesis SuperMix (cat ...
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bioRxiv - Immunology 2020Quote: ... total RNA was isolated from xenograft tissue using the GeneElute Mammalian Total RNA Miniprep Kit (Sigma, Rehovot, Israel) combined with on-Column DNase I Digestion Set (Sigma) ...
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bioRxiv - Genomics 2022Quote: ... Total RNA was extracted using Spectrum™ Plant Total RNA Kit (Sigma-Aldrich, St. Louis, MO, United States) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Total RNA was extracted from freshly ground tissue using the Spectrum™ Plant Total RNA Kit (Sigma Aldrich). RNA samples were used to generate sequence libraries using a library prep kit from New England Biolabs (NEB #E7530) ...
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bioRxiv - Molecular Biology 2023Quote: ... Actin RNA and viral RNA were measured using the KAPA SYBR fast universal qPCR kit (Sigma-Aldrich, US) on the QuantStudio real-time PCR system (Applied Biosystems ...
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bioRxiv - Plant Biology 2023Quote: ... Total RNA from 50 mg FW sporophytes was isolated with the Spectrum Plant Total RNA Kit (Sigma-Aldrich) applying protocol B ...
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bioRxiv - Cell Biology 2024Quote: ... we extracted total RNA with the GenElute™ Mammalian Total RNA Miniprep Kit (#RTN350-1KT, Sigma Aldrich, USA). For both RNA-Seq and WGBS ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Further extraction was done using RNA the Spectrum™ Plant Total RNA Kit (Sigma-Aldrich Chemie GmbH, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Tissue was grounded in liquid nitrogen and RNA was extracted with the Spectrum Plant Total RNA Kit (Sigma) including on-column DNase digestion (Sigma) ...
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bioRxiv - Developmental Biology 2023Quote: Total RNA was extracted from cells using the GenElute™ Mammalian Total RNA Miniprep Kit (Sigma-Aldrich, RTN350). cDNA was reversely transcribed from 1 µg of RNA using the iScript cDNA synthesis kit (Bio-Rad ...
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bioRxiv - Plant Biology 2023Quote: ... RNA from young kiwifruit tissue culture leaves was extracted using the Spectrum Plant Total RNA kit (Sigma-Aldrich), following the manufacturer’s protocol except the incubation temperature of lysed tissue sample was increased to 65°C.
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bioRxiv - Cell Biology 2023Quote: ... Total RNA was isolated from 1 million cells in triplicates using GenElute Mammalian Total RNA Miniprep Kit (Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... Approximately 100mg of tissue was used for RNA extraction using Spectrum Plant Total RNA Kit (Sigma-Aldrich, USA) according to the manufacturer instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... the cells were washed 2× in PBS and then counterstained with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI; 1:10 000; Sigma) for 10 min at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: Time lapse imaging was performed similarly to the static live imaging with the following modifications: 1) 2% fluorescent beads (1:1,000, diameter: 2 μm; Sigma-Aldrich) were added to the low melting point agarose for drift-compensation ...
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bioRxiv - Cell Biology 2020Quote: ... tissue engineered cartilage was digested with papain solution (25 μg/mL papain, 2 mM cysteine, 50 mM sodium phosphate, and 2 mM EDTA adjusted to pH 6.5 [all from Sigma-Aldrich]) at 65 °C for 3h ...
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bioRxiv - Cell Biology 2020Quote: ... 150 mM NaCl, 2 mM EGTA, 2 mM MgCl2, 0.5% NP-40, 1mM DTT, 1U/ml Benzonase from Novagen/EMD Millipore, Temecula ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were permeabilized with 0.5 % TritonX-100 (Wako) in 1x PBS for 1-2 h and blocked in blocking solution (2 % BSA (Sigma-Aldrich), 1 % DMSO ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 2-Heptyl acetate was synthesized by mixing acetic acid with an excess of 2-heptanol and H2SO4 (all Sigma-Aldrich) as catalyst and subsequently heating the mixture for 48 h at 45 °C ...
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bioRxiv - Developmental Biology 2020Quote: ... Cysteine thiols were subsequently reduced and alkylated by adding Tris(2-carboxyethyl)phospin (Carl Roth) to a final concentration of 10 mM and 2-Chloroacetamide (≥ 98.0 %, Sigma-Aldrich) to a final concentration of 40 mM ...
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bioRxiv - Evolutionary Biology 2022Quote: ... A volume of 40 μL (corresponding to 4.106 cells/cm2) of suspension was plated on 6 cm plates filled with 2 mL of 2% Phytagel (Sigma-Aldrich) as previously described by Dubravcic et al ...
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bioRxiv - Neuroscience 2021Quote: ... For immunofluorescence stainings slices were incubated for 1 h at room temperature (RT) in blocking buffer (2% gelatine, 2% BSA (bovine serum albumin, Cat#a2153, CAS Number: 9048-46-8, Sigma), 0.1% Triton X-100 (Merck ...
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bioRxiv - Genetics 2021Quote: Worms were prepared at indicated ages and transferred on a 2% agarose pad with 100 mM sodium azide (DAEJUNG, Siheung, South Korea) or 2 mM levamisole (tetramisole; Sigma). The images of worms were captured by using an AxioCam HRc CCD digital camera (Zeiss Corporation ...
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bioRxiv - Plant Biology 2019Quote: ... in protein sample buffer (50 mM Tris HCl [pH 7.5], 10% glycerol, 2% SDS, 2 mM EDTA, 1 mM DTT and 1% protease inhibitor [Sigma-Aldrich]). Samples were separated by SDS-PAGE on 4-20% gradient polyacrylamide gels and transferred to Immobilon-P PVDF membranes (Millipore ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Neurons were loaded with 2 μM Fura-2-acetoxymethyl ester (Yeasen) dissolved in bath solution and 0.02% Pluronic F127 (Sigma-Aldrich) for 30 min at 37°C ...
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bioRxiv - Physiology 2021Quote: ... and then stained for 2 hours in 1 mL of M9 containing 150 μM 2’,7’-dichlorofluorescein diacetate (DCFDA, Sigma) while rotating in the dark ...
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bioRxiv - Neuroscience 2020Quote: ... we topically applied over the craniotomy 2 μL of PBS containing 25 μM of 2-guanidinoethylmercaptosuccinic acid (GEMSA; Sigma-Aldrich) without removing the electrode ...
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bioRxiv - Cell Biology 2021Quote: ... diluted in 1% normal donkey serum in PBS with 2 µg/mL 4’,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich) for 1 h ...
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bioRxiv - Cell Biology 2021Quote: ... for 15 min and blocked for 2 h at room temperature or overnight at 4°C using 2% BSA (Bovine Serum Albumin, Sigma). Samples were incubated with respective primary antibody (Ab ...
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bioRxiv - Cell Biology 2021Quote: ... for 15 min and blocked for 2 h at RT or overnight at 4°C using 2% BSA (Bovine Serum Albumin, Sigma). Samples were incubated with respective primary antibody (Ab ...
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bioRxiv - Biochemistry 2021Quote: ... Cysteine Biotinylation was carried out using Poly(ethylene glycol) [N-(2-maleimidoethyl)carbamoyl]methyl ether 2-(biotinylamino)ethane (Sigma 757748) (Biotin-maleamide) ...
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bioRxiv - Cell Biology 2021Quote: ... were reared in 10g/l of glucose/200µM of 3BDO (3-Benzyl-5-((2-nitrophenoxy) methyl)-dihydrofuran-2(3H)-one) (Sigma Aldrich) supplemented in standard fly food ...
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bioRxiv - Microbiology 2020Quote: ... All strains were cultured in either rich (YPD: 1% yeast extract, 2% peptone, and 2% dextrose) or complete synthetic minimal (CSM) media (Sigma) lacking appropriate amino acids with 2% dextrose ...