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Citations for Millipore Sigma :
4151 - 4200 of 4275 citations for 6 TERT BUTYLQUINOLINE 98.0+% GC T since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... FISH probes were labelled with 5(6)-carboxyfluorescein-N-hydroxysuccinimide ester (FLUOS) or with the sulfoindocyanine dyes (Cy3) at the 5’ end of the probes (Sigma Aldrich company, Germany). FISH sample preparation was conducted as described earlier (Fernando et al. ...
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Convection and extracellular matrix binding control interstitial transport of extracellular vesiclesbioRxiv - Bioengineering 2022Quote: ... clone-ASC-6, Millipore Sigma; 20 μg/mL anti-α6, clone-GoH3, Thermo Fisher Scientific; 50 μg/mL anti-β1, clone-mAb13, Millipore Sigma; 20 μg/mL anti-β4 ...
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bioRxiv - Cell Biology 2022Quote: ... or 1 ml (6-well plates) of lysis buffer (Tris 50 mM; NaCl 150 mM; pH 7.3; Triton X-100 1 % (Sigma-Aldrich. Cat. Nº: T9284); Leupeptin 5 µM (Focus Biomolecules ...
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bioRxiv - Cell Biology 2022Quote: ... 3 μM-sections were deparaffinized and antigen retrieval was performed in 10 mM citrate buffer (pH = 6; Sigma-Aldrich, St Louis, MO, USA) for 10 min ...
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bioRxiv - Immunology 2022Quote: ... a known volume of trolox standard concentration would result in a similar reduction of the radical 2,2’-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (Sigma-Aldrich, St. Louis, MO). Samples were analyzed in triplicate ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were cultured on a 6-well to confluency and degradation was induced by adding 500 nM dTag-13 (Sigma-Aldrich, SML2601-1MG) or 500 µM auxin (Sigma-Aldrich ...
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bioRxiv - Zoology 2022Quote: The frogs underwent double euthanasia according to institutional ethics guidelines under ethics approval number NWU-00380-16-A5-01: first anaesthesia in 6% ethyl-3-aminobenzoate methansulfonate (MS222) (Sigma-Aldrich Co., USA) and then euthanasia through pithing.
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bioRxiv - Neuroscience 2022Quote: ... Cells were plated on 6-well plates pre-coated with poly-L-lysine (10 μg ml-1, Sigma-Aldrich, St. Louis, MO, USA) and cultivated in DMEM medium (PAA Laboratories GmbH ...
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bioRxiv - Neuroscience 2022Quote: One day before plating, 6-well MEA plates (Axion Biosystems, M384-tMEA-6W) were coated with poly-D-lysine (Millipore Sigma P6407-5MG) in borate buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... Hepatocytes were seeded on polystyrene tissue culture dishes of appropriate size pre-coated by collagen type I (Sigma, C3867, 6 – 10 mg/cm2). The homozygous mutant Wistar rat strain (Gunn-UGT1A1 ...
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bioRxiv - Neuroscience 2023Quote: ... slides were rinsed thrice with 1 x PBS and then stained with 4’,6-diamidino-2-phenylindole (DAPI; 1:10000, Sigma-Aldrich, D9542-10MG) in 1 x PBS solution ...
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bioRxiv - Biophysics 2023Quote: ... The mass of the oligonucleotides was determined using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS, Bruker, AutoFlex Max) using 2’,6’-dihydroxyacetophenone (DHAP, Sigma, Cat. No. 37468) as a matrix ...
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bioRxiv - Genomics 2023Quote: The lentiviral titer was determined by transducing 400,000 cells with increasing volumes (0, 1, 2, 5, 10, 20, and 40 μl) of concentrated virus and polybrene (6 μg/ml; Millipore, TR-1003-G). 48 hours after the transduction ...
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bioRxiv - Cell Biology 2023Quote: ... cover slips were washed with PBS and incubated for 10 min at room temperature with 1 g/mL 4’,6 diamidino-2-phenylindole (DAPI, Sigma Aldrich, Roche, #10236276001) in PBS ...
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bioRxiv - Developmental Biology 2023Quote: Zebrafish larvae were anesthetized with 1 mg/ml Tricaine and transferred to a 6-well glass-bottom plate containing 3% methylcellulose (Sigma-Aldrich, MO, USA) in E3 medium ...
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bioRxiv - Molecular Biology 2023Quote: ... The α-FLAG (clone M2, catalog number F1804) and α-glucose-6-phosphate dehydrogenase (G6PDH, catalog number A9521) were purchased from Millipore Sigma. The α-puromycin antibody (clone PMY-2A4 ...
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bioRxiv - Cancer Biology 2023Quote: ... were deparaffinized and then subjected to antigen retrieval for 10 minutes at 121 uC at 15 PSI in 2.94 g/L sodium citrate (pH 6; Sigma Aldrich, 61332-04-3). Sections were permeabilized in PBS with 0.4% Triton X-100 (PBT ...
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bioRxiv - Neuroscience 2023Quote: ... 315-319 mOsm) with a ceramic blade and placed directly onto 6-well Millipore Millicell cell culture inserts (Millipore Sigma PICM0RG50, Burlington, Massachusetts) over 1 mL warmed neuronal media (1x MEM (Millipore-Sigma) ...
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bioRxiv - Physiology 2023Quote: ... Cells were incubated with P-gp substrate calcein-acetoxymethyl ester (Ca-AM, 177831, 10−6 M, Sigma; 37°C, 5% CO2, 1 hour). Once substrate was added ...
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bioRxiv - Plant Biology 2023Quote: ... 1.5-ml glass vials (Ø×H 11.6 × 32 mm) containing ∼ 100 mg glass wool were filled with 200 µl (Z)-3-hexenyl acetate (HAC, >98%, Sigma-Aldrich, Buchs, Switzerland) and sealed with screw caps containing a rubber septum ...
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bioRxiv - Genomics 2023Quote: ... whole cell lysates were obtained from ∼80% confluent cells in individual wells of 6-well dishes using RIPA lysis buffer (Sigma Aldrich, R0278-500ML) supplemented with Protease and Phosphatase Inhibitor Mini Tablets (Thermofisher ...
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bioRxiv - Molecular Biology 2023Quote: ... and a subsequent 6-day differentiation step in serum-free medium containing 50 ng/ml human brain derived neurotrophic factor (BDNF) (Sigma-Aldrich, Cat# B3795). Cells were seeded at an initial density of 2 × 104 cells/cm2 in 24-well plate coated with Type I collagen (Corning ...
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bioRxiv - Microbiology 2023Quote: ... we also stained cells expressing wild-type and mutant EEEV glycoproteins with a mouse anti-EEEV E1 antibody clone 1A4B.6 (Sigma-Aldrich Cat#: MAB8754) or a mouse IgG2b isotype control (BD Biosciences Cat# ...
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bioRxiv - Bioengineering 2024Quote: ... each well was filled with 400 µL of RPMI-B27 supplemented with 10µM Y-27632 and 5 mg/mL 6-aminocaproic acid (Sigma-Aldrich, cat. no. A7824). On day 1 ...
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bioRxiv - Developmental Biology 2024Quote: The para-nitro derivative of blebbistatin was chosen for myosin inhibition experiments due to its non-phototoxic and non-cytotoxic effects. The drug was purchased as lyophilized powder (Motorpharma, cat.no. 1621326-32-6) and dissolved in 100% DMSO (Sigma-Aldrich, cat. no. D8418) to prepare a stock solution of 10mM concentration that is stored at -200 C ...
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bioRxiv - Neuroscience 2024Quote: ... followed by either a 2 minute (6 µm sections) or 10 minute (50 µm sections) rocking at 37°C in pepsin reagent (ref # R2283, Millipore Sigma, Burlington, MA). The pepsin reaction was quenched by successive incubations in blocking buffer ...
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bioRxiv - Neuroscience 2024Quote: Neuronal processes in octopus arm were labeled with mouse monoclonal antibody 6–11B-1 (1:500 dilution of ascites fluid; Sigma-Aldrich, Cat#: T6793) and mouse monoclonal antibody SMI-31 (1:500 dilution ...
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bioRxiv - Microbiology 2020Quote: ... Primary antibodies were detected with a horseradish peroxidase (HRP)-conjugated secondary antibody (1:3000, #170-6515, Biorad or #12-349, Merck Millipore, in 1x TBS-T with 5 % non-fat dry milk) using enhanced chemiluminescence (ECL ...
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bioRxiv - Cancer Biology 2019Quote: ... and HEK293T embryonic kidney cells [6] were grown in DMEM supplemented with 10% heat-inactivated FBS and 1% penicillin/streptomycin (Sigma Aldrich, St. Louis, MO). All cells were cultured at 37°C in 5% CO2 and 5% O2 ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... the mothers of the experimental animals returned to their home cages and were treated daily with a stainless steel feeding needle per oral gavage with either fluoxetine (10 mg/kg, n= 6 dams) or vehicle (Methylcellulose 1%, (Sigma, St. Louis, MO, USA), n=4 dams ...
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bioRxiv - Bioengineering 2020Quote: ... a suspension of collagen and GAGs were produced by combining microfibrillar, type I collagen (Collagen Matrix, Oakland, NJ) and chondroitin-6-sulfate (Sigma-Aldrich, St. Louis, MO) with calcium salts (calcium nitrate hydrate ...
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bioRxiv - Biophysics 2021Quote: ... The enzyme/protectant mixture was added 1:10 to assay buffer (100 mM Sodium Phosphate, 2 mM Sodium Pyruvate [Sigma-Aldrich], 1 mM NADH [Sigma-Aldrich], pH 6). Enzyme kinetics were measured by NAD+ absorbance at 340 nm ...
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bioRxiv - Neuroscience 2021Quote: ... CAS # 7646-85-7 and ZnC4H6O4; CAS #557-34-6) and arsenic (NaAsO2; CAS #7784-46-5) (all from Sigma-Aldrich, St Louis, MO). The metallic compounds were either dissolved in 30% (w/v ...
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bioRxiv - Cancer Biology 2020Quote: ... 6 cm or 10 cm cell culture dishes or 96 well flat-bottom plates (Thermo Fisher Scientific, Germany; Sigma-Aldrich, Germany; TPP AG, Switzerland). For analyses using the Operetta high content imaging system cells were plated with 10,000-50,000 cells/ml in 96-well µClear plates (Greiner Bio-One ...
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bioRxiv - Cell Biology 2021Quote: ... cells were passaged in a 6 well plates and once confluent transduced with LV-EF1α-rtTA3/PGK-Neomycin plus LV-tetO-MyoD/PGK-Puromycin at 1:1 ratio and supplemented with 6 μg/mL of polybrene (Sigma-Aldrich, TR-1003-G). Transduced MEFs were expanded and selected by sequential antibiotics treatment with MEF medium containing either 1 mg/mL of G-418 solution (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... 132 mM NaCl, 6 mM KCL, 1 mM CaCL2, 1 mM MgSO4, 1.2 mM K2HPO4, 5 mM glucose, Sigma-Aldrich, and 0.4% (w/v) human serum albumin ...
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bioRxiv - Cell Biology 2020Quote: ... for wild type or mutant BAG3-GFP and subjected to 6 h dynein inhibition with Dynarrestin (25μM, SML2332-5MG, Sigma-Aldrich, Saint Louis, MI, USA) or erythro-9-(2-hydroxy-3-nonyl)adenine (EHNA ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed with Tris-buffered saline/Tween 20 and then incubated with an anti-acetyl-α-tubulin antibody (Lysine-40) (Sigma Clone 6-11B1), α-tubulin (Sigma) ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were stained with 1 μg/ml 4′,6-Diamidino-2-phenylindole dihydrochloride in distilled water (D8417; Sigma-Aldrich, St. Louis, MO, USA), cover-slipped ...
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bioRxiv - Neuroscience 2022Quote: ... was performed on a 1 in 6 series of sections from sham- or 6-OHDA-lesioned animal as previously described[57] using an anti-TH mouse monoclonal antibody (LNC1 clone, EMD Millipore, Burlingame, MA, USA). Briefly ...
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bioRxiv - Neuroscience 2022Quote: ... secondary antibodies were applied for 30 min at RT at 1:400 dilution in blocking solution containing 1:10,000 4’,6-diamidino-2-phenylindole (DAPI; Sigma Aldrich, St. Louise, MO, USA) in the closed humid chamber ...
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bioRxiv - Genomics 2022Quote: ... slides were incubated in 1% Kodak PhotoFlo at RT for 1 minute and counterstained with 0.5 mg/mL DAPI (4′,6-diamidino-2-phenylindole, Sigma-Aldrich, St. Louis, MO, USA) in antifade based on DABCO (1,4-diazabicyclo(2.2.2)-octane ...
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bioRxiv - Immunology 2021Quote: Cryopreserved peripheral blood mononuclear cells were thawed and rested for 6 h in R10 with 50 U/mL Benzonase Nuclease (Sigma-Aldrich, St. Louis, MO). They were then stimulated with peptide pools for 12 h ...
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bioRxiv - Bioengineering 2021Quote: ... a suspension of collagen and GAGs were produced by combining microfibrillar, type I collagen (Collagen Matrix, Oakland, NJ) and chondroitin-6-sulfate (Sigma-Aldrich, St. Louis, MO) with calcium salts (calcium nitrate hydrate ...
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bioRxiv - Cell Biology 2022Quote: Cannulated lungs from 17-18-month-old male C57BL/6 mice were filled with warm low melting point agarose (3%, Sigma-Aldrich, St. Louis, MO). Lung segments were cooled on ice for 30 min to allow solidification of the agarose ...
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bioRxiv - Genomics 2022Quote: ... 70% - 80% confluent cells from one well of a 6-well plate were dissociated to obtain single cell suspension and then resuspended in freezing medium (10% DMSO (Sigma Aldrich, Cat. No. D2650), 20% FBS (Millipore ...
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bioRxiv - Microbiology 2019Quote: B. burgdorferi strain B31 was cultured in BSK-H medium (HiMedia Laboratories Pvt. Ltd.) with 6% rabbit serum (Sigma-Aldrich, St. Louis, MO, USA). All culture medium was filter-sterilized by 0.2 µm filter ...
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bioRxiv - Microbiology 2020Quote: ... Cells were disrupted by stirring for 1 hour in lysis buffer (100 mM Tris, 150 mM NaCl, 10% glycerol, 6 mg/mL lysozyme (Sigma, St. Louis, MO, USA), 2 mg/mL deoxycholic acid ...
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bioRxiv - Cell Biology 2021Quote: ... We used following combinations of primary (dilution 1:200) and secondary (dilution 1:250) antibodies: mouse monoclonal anti-acetylated tubulin (Sigma, [6-11B-1], T7451) with Abberior Star 635P goat anti-mouse IgG (H + L ...
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bioRxiv - Microbiology 2021Quote: ... medium (HiMedia Laboratories Pvt Ltd.) was filter-sterilized with 0.2 μm filters and supplemented with 6% rabbit serum (Sigma-Aldrich, St. Louis, MO, USA) for the culturing of B ...