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Citations for Millipore Sigma :
4101 - 4150 of 4275 citations for 6 TERT BUTYLQUINOLINE 98.0+% GC T since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Recombination of the conditional tm1c allele was induced in utero by injecting time pregnant females with a single intraperitoneal (IP) injection of 6 mg tamoxifen (Millipore Sigma, T5648)/40 g body weight dissolved in corn oil ...
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bioRxiv - Bioengineering 2023Quote: ... cells were fixed with 4% paraformaldehyde and the slide was mounted using a medium containing 4′,6-diamidino-2-phenylindole-DAPI (Fluoroshield, Sigma-Aldrich, USA). A fluorescence microscopy setup (Leica DM 2000 LED fitted with a Leica DFC450c camera ...
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bioRxiv - Neuroscience 2023Quote: ... then in the anti-dopamine receptor antibody diluted in the blocking solution for 5-6 days at 4°C (rabbit anti-dopamine D1A receptor, 1:100, Sigma-Aldrich AB1765P). Sections were washed in PBS and incubated for 2 h at RT in the secondary antibody (goat anti-rabbit Alexa Fluor 555) ...
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bioRxiv - Genomics 2024Quote: ... neural crest cells were passaged onto fibronectin-coated 6-well plates (about 1 million cells per well) using Accutase (Sigma-Aldrich A6964) and fed with neural crest maintenance medium (1:1 ratio of DMEM-F12 and neurobasal ...
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bioRxiv - Microbiology 2024Quote: ... Pre-seeded A549 cells were subsequently transduced with the lentivirus in the presence of 6 μg/ml of polybrene (Sigma-Aldrich, TR1003). After 24 hours ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... HEK 293TACE2 and A549ACE2 cells were generated by a transduction with a lentivirus expressing human ACE2 (kind gift from Benjamin Murrel, Karolinska Institutet) in the presence of 6 μg/ml Polybrene (Sigma-Aldrich, H9268). Clones were isolated and selected for homogeneous ACE2 expression as determined by HTM ...
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bioRxiv - Neuroscience 2024Quote: ... the samples were incubated with fluorescent dye-conjugated secondary antibodies at RT for 2 hours with 1% NDS in PBST with 4’,6-diamidino-2-phenylindole (DAPI, 1:1000, Sigma-Aldrich, D9542). Afterward ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated for two days at 4°C with secondary antibody along with 0.01 mg/ml 4′-6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich, Cat#: D9542) to label cell nuclei fluorescently and either 0.2 µl/ml phalloidin-iFluor 594 (Abcam ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were permeabilised overnight at 4°C and then transferred to blocking solution (10% DMSO, 6% normal donkey serum (NDS; Sigma-Aldrich, D9663), 0.2% Triton X-100 in DPBS ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were selected with 5 µg/ml blasticidin S and surviving colonies were isolated using 6 mm Pyrex® cloning cylinder (Sigma-Aldrich). Gene knockout was verified by western blotting of whole cell lysates with appropriate antibodies.
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bioRxiv - Neuroscience 2024Quote: Zebrafish larvae at 5 and 6 dpf were embedded in 1% low-melting temperature agarose diluted into MilliQ water (Sigma-Aldrich, USA). The fish were covered with artificial cerebro- spinal fluid (ACSF ...
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bioRxiv - Developmental Biology 2021Quote: PAs from homozygous a2KO (n = 5) and WT (n = 6) embryos were manually dissected in ice-cold DEPC-treated phosphate-buffered saline (DPBS, Sigma catalog number D8537). Individual PAs were pooled into four samples (WT PA1 ...
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bioRxiv - Neuroscience 2021Quote: ... 2007) was performed following a protocol described earlier with nuclei counterstained by 4’ 6-diamidino-2-phenylindole (DAPI, Sigma, D9542 10MG, 1:1000). To further assess the colocalization of different channels ...
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bioRxiv - Molecular Biology 2020Quote: ... Up to 180mL of blood was collected into 3 60 mL syringes pre-loaded with 6 mL of anticoagulant acid citrate dextrose (ACD) (Sigma C3821 Lot#SLBW6172). Within 15 min of collection ...
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bioRxiv - Cell Biology 2019Quote: Cold KIF3A purification buffer (50 mM NaPi, 400 mM KCl, 2 mM MgCl2 × 6 H2O, 0.001% Brij35, 0.75 mM β-mercaptoethanol, Sigma#M3148, 0.2 mM ATP) supplemented with protease inhibitors and DNaseI was added to frozen BL21-RIL cell pellets expressing a KIF3A construct ...
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bioRxiv - Microbiology 2019Quote: ... The fixed cells were stained with 50 µg mL-1 of 4’,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich, St. Louis, MO, USA) and incubated for 1 h ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and incubated for 30 min with 10 µM 6-chloromethyl-2’,7’-dichlorodihydrofluorescein diacetate (DCFDA) and 1mg/L Hoechst 33342 dye (Sigma Aldrich, United States) diluted in media ...
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bioRxiv - Molecular Biology 2019Quote: ... 5 and 6 were pooled and concentrated to 1 ml using 10 kDa centrifugal filter (Amicon Ultra-2ml, Merck Millipore, Billerica, Massachusetts, USA). The resulting 1 ml sample was loaded on top of a DG ...
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bioRxiv - Genomics 2020Quote: ... We followed the protocol as described previously [6] with one alteration: instead of using one paperdisc (Whatman qualitative filter paper, WHA1001070, SIGMA-ALDRICH CO LTD) per extraction ...
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bioRxiv - Neuroscience 2020Quote: Male CD-1 mice aged 6-10 weeks were given an ip injection of 3 mg/kg LPS from Salmonella typhimurium (Sigma, St. Louis, MO) dissolved in sterile normal saline at 0 ...
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bioRxiv - Biochemistry 2020Quote: ... The production of the (His)6-tagged recombinant proteins was confirmed by Western analysis using mouse monoclonal α-His-horseradish peroxidase conjugated antibodies (Sigma Aldrich, USA). Imaging of the protein bands on the Western blot was conducted using the ECL kit (ThermoScientific ...
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bioRxiv - Neuroscience 2021Quote: ... per well of a 6 well plate of differentiated human neural cultures (~2 months old) along with 3μg/ml of polybrene (Sigma-Aldrich #TR-1003-G), pipetting the LV concentrate directly onto the culture (drop-wise) ...
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bioRxiv - Neuroscience 2020Quote: 100 μg of recombinant Tau 2N4R were incubated for 2h at room temperature in 100 mM sodium citrate pH 6 (Sigma, cat. no. W302600), 100 mM sodium cyanoborohydride (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... primary cortical neurons were cultured for six days in 6-well-plates followed by treatment with the mTOR inhibitor temsirolimus (10 μM in DMSO; Sigma, St. Louis, USA) or Torin 1 (500 nM in DMSO ...
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bioRxiv - Plant Biology 2020Quote: ... and their petioles dipped in 1 mM 5(6)-Carboxyfluorescein diacetate (CFDA, Ex/Em 492/517 nm, Millipore-Sigma, St. Louis, MO, USA) for 5 min ...
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bioRxiv - Plant Biology 2020Quote: ... and their petioles dipped in 1 mM 5(6)-Carboxyfluorescein diacetate (CFDA, Ex/Em 492/517 nm, Millipore-Sigma, St. Louis, MO, USA) for 5 min ...
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bioRxiv - Plant Biology 2021Quote: ... medium, containing 3% sucrose, 10−6 M potassium indole acetate (Nacalai Tesque, Kyoto, Japan) and 10−5 M kinetin (Sigma, St. Louis, MO) at 25 °C ...
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bioRxiv - Cancer Biology 2020Quote: Tetracycline-inducible CALM-AF10 mouse hematopoietic progenitor cells were cultured in 15% FBS DMEM medium without IL-6 and mCSF and treated with either 4 µg/mL doxycycline (DOX) (Millipore Sigma, Billerica, MA) or DMSO for 3 Days ...
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bioRxiv - Cell Biology 2022Quote: ... Coverslips were transferred to 6 well culture dishes and rehydrated with MilliQ water and labeled in H2O for 5 min with Dapi (Sigma-Aldrich, Cat # 9542) 1:10,000 dilution of 1 mg/ml aqueous stock and Syber Gold 1:20,000 dilution of DMSO stock (ThermoFisher Cat # S11494) ...
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bioRxiv - Bioengineering 2022Quote: ... Wild type C57BL/6 mice were sacrificed and were perfused ventricularly with sodium heparin solution in PBS (H3393-100KU, Sigma-Aldrich, MO, USA). The lungs were then excised and fixed in 4% PFA overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: 12- week-old C57BL/6 male mice were injected intraperitoneally with CCl4 (HPLC grade, Cat#270652, Sigma, diluted 1:3 in mineral oil) or mineral oil (control ...
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bioRxiv - Neuroscience 2021Quote: ... Milliplex magnetic bead panel assays (CCL2, CXCL10, IFNγ, IL-1α, IL-1β, IL-2, IL-4, IL-6, and IL-10; Millipore Sigma, Burlington, MA) were used as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: AIA was induced in male and female mice on a C57BL/6 background using the antigen methylated bovine serum albumin (mBSA, Sigma, St Louis, MO) as described previously26 ...
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bioRxiv - Bioengineering 2020Quote: ... HEK293T cells or MSC cells were transduced by overnight exposure to virus stocks and passaged at least five times under puromycin selection (Sigma; 6 µg/ml)
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bioRxiv - Neuroscience 2019Quote: ... mice were gavaged at 4-6 weeks of age with five doses of 100 mg/kg of Tamoxifen (T5648, Sigma, St. Louis, MO) in corn oil (C8267 ...
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bioRxiv - Microbiology 2019Quote: ... and the pellet was resuspended in 0.1 M sodium acetate buffer (pH. 6) and dialysed against four changes of buffer using Amicon ultra centrifugal filters (Merck, Millipore, Tullagreen, Carrigtwohill, IRL). The retentate was recovered and incubated with 100 µl of Micrococcus luteus peptidoglycan (1 mg/ml ...
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bioRxiv - Developmental Biology 2019Quote: Fasting plasma samples collected from offspring at PN30 and during the GTT at 6-7 months of age were analysed for insulin levels using a rat insulin radioimmunoassay kit (SRI-13K, Millipore Australia, Kilsyth, VIC). Insulin samples were run in duplicate at 1:5 ...
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bioRxiv - Biochemistry 2019Quote: ... HEK293 cells were seeded in 6-well plates (3.0 × 105 cells/well) coated with poly-L-lysine (Sigma-Aldrich, St. Louis, MO, USA) and incubated for 24 h ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... female mice received subcutaneous injections (s.c.) of corn oil (CO) (25 ml/kg, vehicle control; n = 6) (#C8267-2.5L, Sigma-Aldrich, St Louise, MO, USA) or a low-dose of TCDD (20 ng/kg/d ...
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bioRxiv - Cancer Biology 2021Quote: We purchased six-week-old C57BL/6 female mice and used a reported chemical carcinogen 4-nitroquinoline 1-oxide (4-NQO, cat#N8141, Sigma, St Louis, MO) to induce ESCC as previously reported [38] ...
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bioRxiv - Bioengineering 2019Quote: ... and 1,2-distearoyl-snglycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (ammonium salt) (DSPE-mPEG 2000, Laysan Lipids, Arab, AL) with 6:1:2:1 ratio in propylene glycol (PG, Sigma Aldrich, Milwaukee, WI) by heating and sonicating at 80 °C until all the lipids were dissolved ...
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bioRxiv - Molecular Biology 2021Quote: ... The cells were infected with the viruses in the presence of 6 μg/mL polybrene (Cat. #107689; Sigma-Aldrich, St. Louis, MO, USA). The overexpressing vectors were transfected into Ink4a/Arf-/- astrocytes using the NeonTM Transfection System 10μl Kit (Cat ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... boric acid and ascorbic acid as well as reagents required for 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) synthesis were obtained from Sigma-Aldrich (Bangalore, India). Acetone was obtained from Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: Each pellet was resuspended in 500 μL (samples collected at 0 and 6 hr) or 650 μL (samples collected at 12 and 24 hr) with chilled (4°C) nuclease-free water (Sigma-Aldrich, Gillingham, UK). The resuspensions were frozen overnight at −80°C ...
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bioRxiv - Evolutionary Biology 2021Quote: ... spun down again and resuspended the cells in 200 µL of 1X PBS (phosphate buffered saline) with 1.5 μl 4′,6- diamidino-2-phenylindole (DAPI, Sigma-Aldrich, 100 ng/ml). DAPI stains dead cells ...
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bioRxiv - Cell Biology 2020Quote: ... As a positive control we used a bona fide Src substrate peptide corresponding to amino acids 6-20 of p34cdc2 (Millipore-Sigma, 12-140). The reactions were carried out at room temperature in a total volume of 25 μL for 40 min in white 96-well ...
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bioRxiv - Microbiology 2019Quote: ... The infected HIE-18 lines were cultured in 75 cm2 flasks containing 12 ml of Schneider’s medium supplemented with 10% FBS at 28°C and subcultured every 5-6 days by splitting at a ratio of 1:2 into new flasks (Sigma Aldrich, NSW, Australia).
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bioRxiv - Molecular Biology 2021Quote: ... were myeloablated 1 day before transplant by intraperitoneal (i.p.) injection with 10 mg/kg of 6-thioguanine (6TG) (Sigma-Aldrich, Saint Louis, MO) or 35mg/kg of Busulfan for mouse m860 ...
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bioRxiv - Molecular Biology 2020Quote: ... Four days after the intravenous injection of 106 iRBC into C57BL/6 mice pre-treated one day earlier with phenylhydrazine 97% (Sigma-Aldrich, 6.18µl/ml) 1 ml of fresh mouse blood was mixed with 10 ml of ookinete medium ...
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bioRxiv - Immunology 2020Quote: ... of 6-8 weeks old were separated into cohorts (10-12 mice) based on antigen: hen egg lysozyme (HEL, Sigma Aldrich, 62971-F), ovalbumin (OVA ...