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Citations for Millipore Sigma :
4051 - 4100 of 10000+ citations for Human Dual Specificity Phosphatase 3 DUSP3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: Nitrite concentration in the lung homogenate was measured (n=3 per group per time point) using Griess Reagent kit following manufacturer instructions (Sigma, St. Louis, MO, USA). Absorbance was recorded using a GloMax plate reader (Promega Corporation ...
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bioRxiv - Physiology 2023Quote: 25-30 mg of frozen powdered liver tissue was used to extract liver lipids as described previously 34 and reconstituted in a tert-butanol-Triton X solution (3:2) and liver triglycerides and cholesterol levels were measured via a commercially available kit (Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from 1.5-week-old Arabidopsis seedlings pooling 3 seedlings/extraction for each transgenic lines and using Spectrum™ Plant Total RNA Kit (Sigma-Al-drich, STRN250), RNAs were on-column treated with the DNase (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... EDTA 5 mM and 1 X-Triton X-100) containing phosphatase and protease inhibitors (Cocktail II, Sigma-Aldrich) to obtain total protein homogenates ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed and incubated at room temperature for 30 minutes with 1μg/ml Streptavidin-Alkaline Phosphatase (Sigma). Plates were washed and developed with p-NitrophenylPhosphate (Sigma) ...
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bioRxiv - Genomics 2019Quote: ... flat and dome-shaped colonies were scored according to morphology followed by alkaline phosphatase staining (Sigma #86R-1KT). For qRT-PCR experiments ...
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bioRxiv - Bioengineering 2021Quote: ... and phosphatase inhibitors (1 mM activated sodium orthovanadate and 1 mM sodium fluoride, Sigma-Aldrich, Oakville, ON, Canada) on ice ...
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bioRxiv - Bioengineering 2020Quote: ... The mouse anti-HA antibody (Covance, Princeton, NJ, USA) and goat anti-mouse IgG-alkaline phosphatase (Sigma-Aldrich) were added to the wells for incubation successively ...
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bioRxiv - Molecular Biology 2021Quote: ... and 0.1% sodium dodecyl sulfate) supplemented with an EDTA-free phosphatase inhibitor cocktail 2 (catalog no. P5726, Sigma) and phosphatase inhibitor cocktail 3 (catalog no ...
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bioRxiv - Cell Biology 2020Quote: ... and protease and phosphatase inhibitor) using a Dounce homogeniser with a tight pestle and incubating with Benzonase (Sigma) for 60 minutes at 4°C before being spun at 12,000 g ...
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bioRxiv - Molecular Biology 2022Quote: ... 1983 [38] with the modification that every buffer was supplemented with Phosphatase Inhibitor Cocktail 2 (Sigma-Aldrich P5726) and Phosphatase Inhibitor Cocktail 3 (Sigma-Aldrich P0044) ...
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bioRxiv - Cancer Biology 2022Quote: Cells were lysed using 5% SDS supplemented with Protease Inhibitor Cocktail and Phosphatase Inhibitor Cocktail 2 (Sigma-Aldrich). The following antibodies were purchased from Cell Signaling Technology (Danvers) ...
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Control of cortical cytoskeleton-membrane interaction by RhoA regulates peripheral nerve myelinationbioRxiv - Neuroscience 2021Quote: ... 1% NP-40, 1 mM EDTA, 0,5% sodium deoxycholate, 0,1% SDS, 1% protease and phosphatase inhibitor cocktails [Sigma]). Samples were left on ice for 30 min ...
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bioRxiv - Immunology 2022Quote: Frozen cell pellets were resuspended in 250-500ul lysis buffer (1% either Digitonin, 1x phosphatase inhibitor cocktail (Sigma), 1x protease inhibitor cocktail (Sigma) ...
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bioRxiv - Systems Biology 2019Quote: ... Animal caps were dissected at stage 11 and lysed in the presence of protein phosphatase inhibitor cocktails (Sigma). Western blots were stained with rabbit anti-Smad1 antibody (1:1000 ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell pellets were lysed using MDS Tris Lysis Buffer (Meso Scale Diagnostics) containing phosphatase inhibitor I (Sigma-Aldrich), phosphatase inhibitor II (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2020Quote: ... Plasmids encoding CaMKII variants were co-transformed with lambda phosphatase into Rosetta 2(DE3)pLysS competent cells (Millipore). Expression was induced overnight with 1mM Isopropyl β-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Pathology 2020Quote: The kidneys were lysed using RIPA lysis buffer containing protease inhibitors and phosphatase inhibitor cocktail 2 (Sigma-Aldrich), and processed by Western blots as described before [3] ...
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bioRxiv - Molecular Biology 2020Quote: ... 100 mM NaCl and 1 mM dithiothreitol) supplemented with a cocktail of protease and phosphatase inhibitors (Sigma-Aldrich) for 10 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... cells were harvested and lysed in 0.1% NP-40 with phosphatase inhibitor cocktails (Sigma-Aldrich, St. Louis, MO). After brief sonication ...
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bioRxiv - Neuroscience 2021Quote: ... After the reaction with alkaline phosphatase-labelled goat anti-rabbit IgG (Sigma; 1:1,000 dilution in dilution buffer), immunoreactive products were obtained in a substrate solution of p-nitrophenylphosphate (SIGMA FAST™ p-nitrophenyl phosphate tablet set ...
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bioRxiv - Microbiology 2021Quote: ... The plates were incubated with anti-mouse IgG conjugated with alkaline phosphatase (whole molecule) (Sigma-Aldrich A-3688) diluted 1:2000 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Membranes were then washed three times with TBS-T and incubated with alkaline phosphatase-conjugated secondary antibodies (Sigma) in 5% milk in TBS-T for 30 min at room temperature ...
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bioRxiv - Biochemistry 2021Quote: Cells expressing GRAMD1C-EGFP or GRAMD1C(ΔGRAM)-EGFP were lysed with NP-40 lysis buffer (150 mM, 1.0% NP-40, 50 mM Tris-HCl pH 8.0) supplemented with PhosStop phosphatase inhibitor (Sigma) and complete protease inhibitor cocktail (Sigma) ...
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bioRxiv - Immunology 2021Quote: ... 50 µl/well of the alkaline phosphatase-conjugated anti-mouse IgG was added (Sigma-Aldrich, St Louis, MO), it was diluted at 1:1000 in a PBS/BSA 2% solution ...
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bioRxiv - Cell Biology 2021Quote: 2x Laemmli buffer was added to the adherent cells and 2uL of phosphatase inhibitor cocktail 2 (Sigma, P5726) and 2uL of phosphatase inhibitor cocktail 3 (Sigma ...
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bioRxiv - Biochemistry 2020Quote: ... acid phosphatase (AP) and β-glucosidase (BGL) from almonds were purchased from Sigma-Aldrich (St. Louis, MO, USA). Dithiothreitol (DTT) ...
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bioRxiv - Microbiology 2022Quote: ... Then 1 mL of lysis buffer (8 M Urea buffer, 50 mM NH4HCO3, phosphatase inhibitors (Sigma-Aldrich, P2745), and protease inhibitors cocktail (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... as primary and a goat-anti-mouse antibody conjugated to an alkaline phosphatase (dilution 1:10,000; Sigma, A3562) as secondary antibody ...
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bioRxiv - Microbiology 2022Quote: ... The presence of over-expressed protein was confirmed using monoclonal anti-poly histidine alkaline phosphatase antibody (Sigma-Aldrich) at 1:20,000 dilution.
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bioRxiv - Pathology 2022Quote: ... respectively an anti-rat IgG conjugated to alkaline phosphatase (1:500) (Sigma-Aldrich®, Merck KGaA, Darmstadt, Germany), for 60 minutes ...
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FTSH3 facilitates complex I degradation through a direct interaction with the complex I subunit PSSTbioRxiv - Plant Biology 2023Quote: ... and 10 μL of protease inhibitor (protease and phosphatase inhibitor cocktail dissolved in 1 mL water) (Sigma Aldrich), and incubated on ice for 30 min.
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bioRxiv - Neuroscience 2022Quote: ... Color reaction was performed after incubation with anti-DIG antibody conjugated to alkaline phosphatase (Sigma-Aldrich Cat#11093274910) using NBT/BCIP substrate (Roche Cat#11697471001) ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.2% NP40) supplemented with 1% protease inhibitor cocktail set III and 1% phosphatase inhibitor cocktail set II (Millipore). Following clarification by centrifiguation ...
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bioRxiv - Biochemistry 2023Quote: ... To the cooled sample was added 0.1U of P1 nuclease 0.1U acid phosphatase (Sigma Aldrich, St. Louis, MO.), and 0.01U RNase A (Worthington Biochemical) ...
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bioRxiv - Molecular Biology 2023Quote: Metabolic quenching and nucleotide phosphate metabolite pool extraction were performed by adding 1 ml ice-cold 2:2:1 acetonitrile:methanol:water with 0.1% formic acid with 1x MS-Stop phosphatase inhibitor (Sigma-Aldrich). Samples were spiked with 10 µl 100 mM mix of adenosine-15N5-5′-triphosphate ...
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bioRxiv - Immunology 2023Quote: ... for 1 hour and then alkaline phosphatase substrate solution containing 4-nitro-phenyl phosphate (N2765-50TAB, Sigma-Aldrich) for approximately 30min ...
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bioRxiv - Cell Biology 2023Quote: ... 300 mM sucrose, 1.5 mM MgCl2, 5 mM EDTA, 0.5% Triton X-100, 1× protease and phosphatase inhibitor, Sigma) and incubated on ice for 10 min ...
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bioRxiv - Cell Biology 2023Quote: ... containing 10 µg/ml LPC (leupeptin, Pepstatin A, and chymostatin) and 1% phosphatase inhibitor cocktail 2 (P5726, SIGMA). Following clarification of the lysate by centrifugation at 15,000 rpm (21,000 g ...
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bioRxiv - Immunology 2023Quote: ... and plates were probed with IgG1-and IgG2a-specific secondary alkaline phosphatase (AP)-conjugated detection antibodies (Sigma-Aldrich #SAB3701172 and #SAB3701179 ...
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bioRxiv - Cell Biology 2023Quote: Cells and tissues were lysed in cell extraction buffer with protease and phosphatase inhibitor cocktail (P8340, Sigma-Aldrich). Cell lysates were cleared at 20,000g for 10 min at 4°C and protein concentration determined using BioRad protein assay ...
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bioRxiv - Plant Biology 2024Quote: ... Commercial alkaline phosphatase (AP)-conjugated goat anti-rabbit (IgG) antibodies (Sigma-Aldrich, USA, diluted 1:5,000 in PBS) were added and incubated for 2 h at 37°C ...
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bioRxiv - Plant Biology 2024Quote: ... 0.5 mg TTN5 protein was combined with 1 U of agarose bead-coupled alkaline phosphatase (Sigma Aldrich, Germany) for degradation of bound GDP to GMP and Pi in the presence of 1.5-fold molar excess of non-hydrolyzable GTP analog GppCp (Jena Bioscience ...
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bioRxiv - Cell Biology 2024Quote: ... and osteoblast-specific alkaline phosphatase activity was measured at 405 nm by using 120 mM p-nitrophenolphosphate (Sigma) in 0.1 M Tris buffer (pH 8.5) ...
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bioRxiv - Cancer Biology 2024Quote: ... 150 mM NaCl) containing a protease and phosphatase inhibitor cocktail (cOmpleteTM, EDTA-free Protease Inhibitor Cocktail, Millipore Sigma, #COEDTAF-RO ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were then washed twice 5 min in TBST and incubated with the primary antibody over night at 4°C (human and mouse a-syn: 610786 BD Biosciences; human α-syn: 804-258-1001, Enzo Life Science; beta-actin: A4700, Sigma). After incubation with the first antibody ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies for immunostaining included rabbit anti-human/mouse PKD-1 (SAB4502371) and mouse anti-human/mouse α-smooth muscle actin (α-SMA, A2547) (Sigma-Aldrich), mouse anti-human/rat CD44 (5640S ...
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bioRxiv - Developmental Biology 2024Quote: ... anti-Ku80 (CST, #2180, 1:400), anti-human nuclear antigen (Novus NBP2-34342, 1:200) and anti-human-specific Albumin (Sigma, A3293, 1:200). Species-specific secondary antibodies conjugated with Alexa Fluor 555 ...
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bioRxiv - Immunology 2023Quote: ... Cytokine levels in matched BAL fluid and plasma collected from patients were measured using the multiplexed human cytokine/chemokine magnetic bead kits from Millipore (HCYTMAG-60K-PX41 and HCYP2MAG-62K) according to manufacturer’s protocol (HCYTOMAG-60K Rev ...
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bioRxiv - Immunology 2019Quote: ... scFv secretion levels were analyzed by ELISA after coating with HEL antigen (Sigma-Aldrich, 62971, 4 μg ml-1) in PBS (Thermo ...