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Citations for Millipore Sigma :
3951 - 4000 of 10000+ citations for Recombinant Human MSLN Protein Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: Approximately equal amounts of GST fusion proteins and alanine-mutated GST fusion proteins were separated by 12% SDS-PAGE and transferred to PVDF transfer membranes (Millipore, MA, USA). The membranes were blocked with 5% skimmed milk powder for 2 hours at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... the expression levels of recombinant B56 subunit and PME-1 were monitored by western blot using antibodies that specifically recognize HA-tag (Sigma, 2CA5, Cat#: 116666060011, 1:1000) and PME-1(Abcam ...
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bioRxiv - Plant Biology 2020Quote: ... Prior to labelling the apoplastic fluid was incubated for 30 min with different concentrations of recombinant LpCys1 or chicken egg white cystatin (CEWC, Sigma-Aldrich, St. louis, Mississippi, USA) and Tris-HCl (24 mM Tris ...
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bioRxiv - Biochemistry 2024Quote: ... the chamber was rinsed three times with T50 to remove non-specific interactions and a protocatechuic acid/recombinant protocatechuate-3,4-dioxygenase (Sigma Aldrich and Oriental Yeast Co., respectfully) (PCA/rPCD ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were transferred onto an immobilon-FL membrane (Millipore) at 75 V for 1hr ...
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bioRxiv - Physiology 2021Quote: ... Proteins were transferred to a PVDF membrane (Merk Millipore), which was blocked with 5% BSA in PBS containing 0.1% Tween-20 (0.1% PBST ...
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bioRxiv - Biophysics 2021Quote: ... FnCas9 protein was purchased from Millipore Sigma (FNCAS9PROT-250UG).
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bioRxiv - Biophysics 2020Quote: Proteins were concentrated for structural studies using ultrafiltration (Sigma-Millipore ...
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bioRxiv - Molecular Biology 2020Quote: Proteins were expressed in Rosetta 2 cells (EMD Millipore) grown in LB at 37°C and induced at an OD600 of 0.7 with 0.5 mM IPTG for 16 hours at 18°C ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were wet transferred onto PVDF membrane (Millipore, IPFL00010), blocked with 5% skimmed milk and probed successively with anti-Flag (Agilent ...
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bioRxiv - Microbiology 2021Quote: ... beads containing bound proteins were incubated with thrombin (Novagen) overnight at 20°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were transferred to Immobilon-FL PVDF membrane (Millipore), and membranes were incubated in Odyssey blocking buffer (LI-COR Biosciences ...
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bioRxiv - Plant Biology 2022Quote: ... Protein concentration in S3 was normalized via Bradford (SIGMA) to ensure that equal amount of protein was loaded before ultracentrifugation step ...
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bioRxiv - Molecular Biology 2022Quote: ... protein complex were eluted with 10mM Maltose (Sigma Aldrich). Resolve elutes by SDS-PAGE and transfer to PVDF membrane for western blots ...
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bioRxiv - Synthetic Biology 2022Quote: ... The protein sample was transferred to PVDF membranes (Millipore) using Mini Trans-Blot (Bio-Rad) ...
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bioRxiv - Biochemistry 2022Quote: ... Protein estimation was performed using Bradford assay reagent (Sigma).
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bioRxiv - Neuroscience 2021Quote: ... Antibodies were bound to protein G-Sepharose (Sigma-Aldrich) in RSB-100 buffer containing 0.1% NP40 ...
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bioRxiv - Molecular Biology 2021Quote: Protein mixtures were Trichloroacetic acid (Sigma Aldrich, Cat# T0699) - precipitated overnight at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... protein bands were visualized with Ponceau stain (Sigma, P7170) to confirm protein transfer and even loading.
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bioRxiv - Molecular Biology 2021Quote: ... and proteins were transferred to nitrocellulose membranes (Sigma #GE10600002). Western blot analysis was performed under standard conditions with antibodies against Tubulin (Sigma #T9026 ...
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bioRxiv - Molecular Biology 2020Quote: ... proteins were transferred onto Immobilon-FL PVDF membranes (Millipore) and membranes were blotted with Anti-Flag M2 antibody (Sigma ...
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bioRxiv - Biophysics 2022Quote: ... proteins were transferred to Nitrocellulose membranes (GE10600012, Sigma-Aldrich). Nitrocellulose membranes were blocked with 5% (w/v ...
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bioRxiv - Microbiology 2020Quote: ... 75 μl pre-washed protein A sepharose (Sigma-Aldrich) was added for recovery of FLAG-antibody and incubated for 30 min ...
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bioRxiv - Genomics 2020Quote: ... Proteins were transferred to a nitrocellulose membrane (Merck Millipore) for 1h at 20V ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein G Sepharose (Millipore Sigma Cat. GE17-0618-01) preblocked in 5% BSA was added and incubated for 1 additional hour rotating at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Protein concentration was determined using Bradford reagent (Sigma-Aldrich). Total protein extracts (30 μg ...
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bioRxiv - Cancer Biology 2019Quote: ... phosphorylated SR proteins (clone 1H4, 1:500, Millipore, #MABE50), total SR proteins (1;1000 ...
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bioRxiv - Developmental Biology 2019Quote: ... Protein concentration was determined using Direct Detect spectrometer (Millipore). 20 µg of protein per sample was denatured at 70°C for 10 mins in 1× LDS loading buffer ...
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bioRxiv - Bioengineering 2020Quote: ... After concentrating the protein solution with Centriprep concentrators (Millipore), the final working concentration of protein was found to be 100 μg/ml using a BCA Protein Assay kit (Thermo Scientific) ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were eluted off by stripping buffer (Millipore 2504) and re-probed for total Cx43 using the Sigma anti-rabbit antibody ...
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bioRxiv - Plant Biology 2019Quote: ... the protein concentration was estimated by Bradford (Sigma-Aldrich) and SDS-PAGE analysis (Supplementary Figure S1).
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bioRxiv - Biochemistry 2019Quote: ... Protein was then estimated using Bradford’s reagent (Sigma, USA).
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bioRxiv - Molecular Biology 2019Quote: EZ-Magna RNA-binding protein immunoprecipitation kit (Millipore, USA) was performed according to the manufacture’s protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein emulsion was made in complete Freund’s adjuvant (Sigma) for subcutaneous immunization in two rabbits followed by three successive boosters in incomplete adjuvant at four week intervals.
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bioRxiv - Biophysics 2019Quote: ... Protein estimation was done using Bradford reagent (Sigma Inc.) and stored in −80°C after flash freezing in liquid nitrogen.
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bioRxiv - Biochemistry 2019Quote: ... The supernatants were passed through a Protein A (Sigma) affinity column ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... Proteins were transferred to Immobilon-FL PVDF membranes (Millipore) followed by incubation in Odyssey blocking buffer (LI-COR ...
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bioRxiv - Plant Biology 2019Quote: ... After incubation with Protein A agarose beads (Millipore, USA), enriched DNA fragments used as the templates for PCR were eluted out and purified with phenol/chloroform (1:1 ...
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bioRxiv - Cell Biology 2019Quote: ... Protein G Sepharose 4 Fast Flow (P3296 Sigma-Aldrich) were incubated with 2 µg of GFP antibody raised in Goat for 2h at 4°C and were then washed with lysis buffer ...
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bioRxiv - Plant Biology 2019Quote: ... and Protein G magnetic beads (EMD Millipore, Burlington MA) pre-treated with 0.1% (w/v ...
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bioRxiv - Immunology 2019Quote: ... and 20 μl of Magna protein A beads (Millipore). Bound Saa3 promoter sequences were quantified using SYBR Green-based real-time PCR (target sequence and primers listed in Table S4) ...
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bioRxiv - Bioengineering 2019Quote: ... microtubule associated protein 2 (MAP2) (1:500, Sigma-Aldrich). The cells were subsequently washed 6 x 5 min each with PBS/0,1% Triton-X 100 ...
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bioRxiv - Biochemistry 2019Quote: ... Protein was eluted with 10 mM reduced GSH (Sigma) dissolved in 10 mM Tris-HCl ...
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bioRxiv - Plant Biology 2021Quote: ... The eluted protein was concentrated using amicon filters (Millipore). The concentrated protein was then passed through a Q-sepharose (Ion-exchange column ...
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bioRxiv - Genetics 2020Quote: Cellular protein was extracted using RIPA buffer (Sigma, #R0278) with protease inhibitors (Roche ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein concentration was quantified by Bradford (Sigma B6916-500mL) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... Biotinylated hyaluronan-binding protein (HABP) was supplied by Millipore-Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... Protein concentration was determined using Bradford Reagent (Sigma-Aldrich). At experimental endpoints ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred to an Immobilon PVDF membrane (Millipore) overnight at 50 milliamps ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were then transferred onto PVDF membranes (Immobilon, Millipore) and incubated overnight with primary antibodies at 4°C ...