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Citations for Millipore Sigma :
4001 - 4050 of 10000+ citations for Recombinant Human MSLN Protein Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... Total protein concentration was assessed using Bradford assay (Sigma) and spectrophotometrically (considering ε515 = 83,000 M-1 cm-1 for Ca2+-free form of cpVenus) ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were resuspended in 5μL of 8M urea (Sigma) in 100mM ammonium bicarbonate for 10min at 30°C ...
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bioRxiv - Biochemistry 2020Quote: ... protein translation was inhibited with cycloheximide (CHX, Sigma-Aldrich) dissolved in phosphate buffer saline (PBS ...
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bioRxiv - Biochemistry 2020Quote: ... Protein melting was monitored using 1000X SYPRO Orange (Sigma) binding dye ...
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bioRxiv - Biochemistry 2020Quote: ... pH 7.0 and SYPRO Orange protein stain dye (Sigma). Melting curves were recorded with a temperature range of 20 to 95 °C ...
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bioRxiv - Neuroscience 2019Quote: The protein synthesis inhibitor cycloheximide (CHX, Sigma-Aldrich C7698) (Pedreira ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were electroblotted onto PVDF membranes (IPVH00010, Merck Millipore) via a semi- dry cassette (1703940 ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were reduced with dithiothreitol (DTT, 5 mM; Sigma) at 55°C for 30 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein and RNA were degraded by Proteinase K (Sigma) and RNase A (5prime) ...
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bioRxiv - Cancer Biology 2020Quote: ... 40 μl of protein A/G Sepharose beads (Millipore) were added and the lysate was pre-cleared for 1 hr at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... proteins were transferred to Immobilon-FL PVDF membranes (Millipore) using the NuPage transfer buffer transfer system (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... Npas4 protein was affinity purified on M2 agarose (Sigma) as described previously54 ...
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bioRxiv - Biochemistry 2020Quote: ... For the protein used for electron microscopy thrombin (Sigma) 1:1 w/w was added during dialysis to remove the affinity label) ...
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bioRxiv - Cell Biology 2021Quote: ... proteins were transferred onto a PVDF membrane (Millipore #ISEQ20200) and incubated 1 hour at room temperature with 2% milk powder in PBS ...
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bioRxiv - Plant Biology 2021Quote: All tested proteins and citrate synthase (CS, Sigma, C3260) was dialyzed in 20 mM HEPES-KOH ...
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bioRxiv - Microbiology 2020Quote: ... filtered through a 0.45μm low protein-binding filter (Millipore), and used to transduce Vero cells ...
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bioRxiv - Plant Biology 2021Quote: ... coli BL21 (DE3) for protein production (Novagen, Madison, USA).
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bioRxiv - Plant Biology 2021Quote: ... Retained proteins were alkylated with 50 mM acrylamide (Sigma) in UT buffer for 30 minutes at 20◦C while gently shaking ...
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bioRxiv - Plant Biology 2021Quote: ... proteins were wet-transferred to a nitrocellulose membrane (Millipore). Membranes were blocked in 5% skimmed milk and probed with anti-HA (Roche anti-HA-HRP 3F10) ...
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bioRxiv - Plant Biology 2021Quote: ... The total proteins were quantified by Bradford reagent (Sigma) and purity was analysed by SDS-PAGE ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were transferred onto an immobilon-FL membrane (Millipore) at 75v for 1hr ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-protein kinase C-α (PKCα) (Sigma, P4334); rabbit anti-VSX2 (Chx10 ...
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bioRxiv - Immunology 2021Quote: ... Proteins were then transferred to PVDF membrane (Millipore, 0.45μm). The membranes were first incubated with anti-phospho-Src (Tyr416 ...
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bioRxiv - Immunology 2021Quote: ... Proteins were transferred onto Immobilon-FL PVDF membranes (Millipore), and nonspecific binding was blocked with 3% BSA in Tris-buffered saline for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... proteins were precipitated with trichloroacetic acid (TCA; Sigma-Aldrich) at a final concentration of 5% and washed twice with cold acetone ...
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bioRxiv - Neuroscience 2020Quote: ... or mHTT protein (1-82aa, Millipore, MAB5492, 1:500), and the appropriate biotinylated goat anti rabbit or goat anti mouse or secondary antibody (Vector Laboratories ...
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bioRxiv - Plant Biology 2021Quote: ... Protein A Mag Sepharose (Sigma-Aldrich #28-9440-06) beads were washed with RIPA buffer (10 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Plant Biology 2020Quote: ... subsequently the proteins were reduced with dithiothreitol (DTT, Sigma), alkylated with iodo-acetamide (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... Purified proteins were concentrated in Amicon Filter Units (Millipore) before purification by size exclusion chromatography using a HiLoad 26/600 Superdex 75 column (GE Healthcare).
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bioRxiv - Microbiology 2020Quote: ... separated proteins were further transferred onto PVDF membranes (Millipore). The membrane was blocked with 5% fat-free milk in TBST for 2 h at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... proteins were blotted onto polyvinylidene difluoride membranes (PVDF; Millipore) and blocked in blocking buffer (1:1 ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies to basement membrane proteins collagen-IV (Merck-Millipore) or perlecan (Abcam) ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were visualized using chemiluminescence detection reagent (ECL, Millipore). Protein levels in each solubility fraction were quantified densitometrically and expressed as percentage of the total level (i.e ...
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bioRxiv - Cancer Biology 2020Quote: ... IF and IHC: Prosurfactant protein (SPC) pAb (Millipore, AB3786), Uteroglobin (CC10 ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were then transferred onto a nitrocellulose membrane (Sigma). The membrane was blocked with 3% milk in PBS ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred onto PVDF membranes (0.45µm, Merck Millipore) at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... the target proteins were detected using ImmobilonTM Western (Millipore). Information regarding the primary antibodies and secondary antibodies is described in Supplementary Table S1.
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bioRxiv - Cell Biology 2020Quote: ... Anti-Glial Fibrillary Acidic Protein (1/1000, Millipore, MAB360). The next day ...
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bioRxiv - Cell Biology 2021Quote: Biotinylated hyaluronan-binding protein (HABP) was supplied by Millipore-Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred on to Immobilon-FL PVDF (Sigma) membrane using a wet transfer XCell IITM Blot Module system (120 mins ...
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bioRxiv - Biophysics 2022Quote: Proteins were assembled with 1.6 mM IP6 (Sigma-Aldrich) and 1.4 mM BVM (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2022Quote: ... Protein concentration was quantified with a Bradford assay (Sigma). The following primary antibodies were used ...
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bioRxiv - Cell Biology 2022Quote: ... The protein was transferred to PVDF membranes (Millipore, USA) after electrophoresis and blocked for 1 h with blocking buffer (Beyotime ...
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bioRxiv - Biochemistry 2022Quote: Protein lysates were harvested in RIPA buffer (Sigma, R0278) supplemented with protease inhibitors (Fisher ...
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bioRxiv - Genomics 2022Quote: ... Magna ChIP Protein A and G magnetic beads (Millipore) were washed twice with TE 0.1% SDS and 1% TritonX and added to the lysate to preclear for 1h at 4°C rotating ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein was wet transferred to nitrocellulose membranes (Sigma, GE10600001) in transfer buffer (25mM Tris base ...
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bioRxiv - Cancer Biology 2022Quote: ... then incubated with magnetic Protein A/G beads (Millipore) for 2 h at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein was purified using anti-Flag M2 beads (Sigma) and resuspended in Buffer D (200 mM KCl ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were precipitated with trichloroacetic acid (TCA; Sigma-Aldrich) at a final concentration of 5% and washed twice with ice-cold acetone ...
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bioRxiv - Biochemistry 2022Quote: ... and CAHS12 proteins were expressed using pET system (Novagen) essentially as described previously [15] ...