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Citations for Millipore Sigma :
3951 - 4000 of 10000+ citations for Biotin Z Antibody Internalization Kit mouse since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... The ratio of protein level or phosphorylation levele against control β-actin protein level (detected with a mouse monoclonal Anti-β-Actin-Peroxidase antibody (A3854 Sigma)) was normalized to untreated wt sample mean.
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bioRxiv - Microbiology 2020Quote: ... was used for Western blotting at a dilution of 1:4,000 and mouse monoclonal antibody against β–actin (Sigma, St. Louis, MO) was used for Western blotting at a dilution of 1:2,000 ...
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were blocked with Odyssey blocking buffer (Li-COR) followed by incubation with antibodies specific for GFP (mouse monoclonal anti-GFP [7.1 and 13.1] (Sigma-Aldrich Cat# 11814460001, RRID:AB_390913)) and Tub1 (rat monoclonal anti-tubulin alpha ...
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bioRxiv - Cancer Biology 2020Quote: ... 50 μL of empty Dynabeads® Protein G was washed by Citrate-Phosphate Buffer (pH 5.0) and then incubated with 5ug Mouse Monoclonal anti-PIWIL1 antibody (Millipore SIGMA, Cat# SAB4200365) for 4 hours at 4°C with rotation ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were blocked with 5% bovine serum albumin in tris-buffered saline/0.1% [vol/vol] tween-20 (TBS-T) before probing overnight at 4°C with antibodies raised to the following targets: mouse anti-puromycin (Millipore-Sigma, MABE343), mouse anti-OC43 N (CoV antibody ...
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bioRxiv - Bioengineering 2019Quote: ... MA) and the secondary antibody used was HRP-conjugated goat polyclonal anti-mouse IgA (1:500; A4789; Sigma Aldrich; St. Louis, MO). HRP was detected with 1-Step Ultra TMB-ELISA Substrate Solution (Thermo Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies were used at 1:1000 and included: mouse monoclonal anti-β1/β2-adaptins (clone 100/1; Sigma, St. Louis, MO); mouse monoclonal anti-clathrin heavy chain (clone 23 ...
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bioRxiv - Pathology 2019Quote: ... membranes were incubated for 30 min at room temperature in PBS-T containing 5% milk powder and secondary antibody: peroxidase-conjugated anti-mouse IgG (1:5000) (Sigma-Aldrich, A2304). The membranes were then washed in PBS-T ...
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bioRxiv - Microbiology 2019Quote: ... plates were incubated with horseradish peroxidase-labelled goat anti-mouse IgG antibody (Sigma-Aldrich, cat # AP124P, diluted 1:3000 in PBS-T) for 1 h ...
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bioRxiv - Microbiology 2020Quote: ... The primary antibody used for Western blot was a horseradish peroxidase conjugated goat anti-mouse IgG (Sigma-Aldrich, St. Louis, MO, USA).
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bioRxiv - Pathology 2020Quote: ... Proteins were immunoblotted with antibodies against FECH (rabbit, 1:400, LS Bio, #LSC409953, and β-actin (mouse, 1:5000, Sigma-Aldrich, AC40). Secondary antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... The following secondary antibodies (1:100) were applied for 2 h at room temperature on a rotating platform: Atto 594 (anti-mouse, Sigma-Aldrich, 76085) and Abberior STAR 635 P (anti-rabbit ...
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bioRxiv - Systems Biology 2020Quote: ... Blots were incubated with appropriate secondary antibodies (Anti-Rabbit IgG-Peroxidase Sigma-Aldrich A0545 and Anti-Mouse IgG-Peroxidase Sigma-Aldrich A9044) and imaged using GE Amersham Imager 600.
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bioRxiv - Microbiology 2021Quote: ... Wells were washed again and probed with 100 μl/well of a α-mouse alkaline phosphatase-conjugated secondary antibody (Sigma Aldrich, A4187) diluted 1:2,000 and incubated for one hour at room temperature ...
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bioRxiv - Systems Biology 2020Quote: ... Resolved proteins were transferred to nitrocellulose membranes and detected by standard Western blotting using monoclonal anti-FLAG M2-mouse primary antibody (Sigma Aldrich, F1804) and HRP labelled anti-mouse IgG secondary antibody (Cell Signalling technology ...
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bioRxiv - Plant Biology 2021Quote: ... The glutathionylated VirE2 was detected by the anti-glutathione monoclonal primary antibody (My biosource, 1:1000) and anti-mouse (Sigma, 1:1000) secondary antibody (Klaus et al ...
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bioRxiv - Genomics 2021Quote: ... Cells were immunostained with rabbit antibody against Histone H3 (phospho S10) (1: 5000) to identify mitotic cells and mouse anti-Tubulin (Sigma; 1:500) to assay cell viability and mitotic stage as previously described55 ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were rinsed 3 times with PBS for 10 min each and blocked with 5% goat serum and 0.3% Triton X-100 for 2 hours at room temperature and incubated for overnight at 4°C with following primary antibodies: anti-calbindin (1:1000, mouse, Sigma Aldrich, #C9848). After 3 rinses with PBS for 10 min ...
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bioRxiv - Genomics 2021Quote: ... Eluted proteins were then analyzed by western blotting with anti-U1-70k mouse monoclonal antibody (9C4.1) (05-1588, Merk Millipore, Burlington, MA, USA) at a dilution of 1:500 for the detection of U1-70k ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein detection was achieved using the following antibodies: anti-Toddler (rabbit, 1:500, provided by the Reversade Lab11) and anti-tubulin (mouse, 1:10000, Sigma-Aldrich T6074).
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bioRxiv - Neuroscience 2021Quote: ... Cells were blocked for 4 hrs at 4°C, incubated with primary antibodies for Isl1/2 (mouse, DSHB 39.4D5, 1:500) and Peripherin (rabbit, Millipore AB1530, 1:200) overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies (diluted in 5% BSA in 1X PBS-T) were as follows: Mouse anti-β-Actin (1:10,000, Sigma-Aldrich, A5441), Rat anti-α Tubulin (1:2000 ...
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bioRxiv - Cell Biology 2021Quote: ... then washed twice in PBS and incubated 1 hour with the tertiary antibody goat anti-mouse whole molecule conjugated to FITC (Sigma, 1:400). The intrabody anti-GFP nanobody 3 x HA-tagged (INbGFP::3HA ...
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bioRxiv - Cell Biology 2021Quote: ... membranes were then cut based on size and incubated overnight with the following primary antibodies: 1 μg/mL mouse α-alpha-tubulin (EMD Millipore), 1 μg/mL mouse mAb414 (Biolegend) ...
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bioRxiv - Cancer Biology 2020Quote: ... 50 μL of empty Dynabeads® Protein G was washed by Citrate-Phosphate Buffer (pH 5.0) and then incubated with 5ug Mouse Monoclonal anti-PIWIL1 antibody (Millipore SIGMA, Cat# SAB4200365) or 5ug Normal Mouse IgG Polyclonal Antibody (MERCK ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were analysed using 4-12% Bis-Tris polyacrylamide gels in MOPS buffer followed by Coomassie staining or western blot using the following antibodies: Mouse anti-FLAG M2 (Sigma, 1:10,000). RNA was isolated from TriReagent according to the manufacturer’s protocol and then reverse transcribed using the high capacity RNA-to-cDNA kit (Applied Biosystems ...
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bioRxiv - Developmental Biology 2022Quote: ... and cultures were incubated overnight at 4°C with primary antibodies diluted in TBS/10% goat serum: mouse anti-β-tubulin-III (Sigma #T8660) or mouse anti-MAP2 (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... Then membranes were incubated with anti-mouse or anti-rabbit horseradish peroxidase conjugated secondary antibodies (1:3000, Sigma Aldrich, St. Louis, MO) for 1 hr ...
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bioRxiv - Neuroscience 2023Quote: ... The membrane was washed with 0.2% SDS and retained SDS-insoluble mHTT aggregates were detected with mouse anti-polyQ antibody (Millipore, MAB1574, 1:5,000).
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bioRxiv - Neuroscience 2022Quote: ... Larvae were washed three times in 0.1% PBST and incubated with secondary antibodies: anti-mouse Alexa Flour 647, 1:100 (Invitrogen, #28181. Stained larvae were mounted using DAPI Fluoroshield (Sigma-Aldrich, #F6182). Images were collected on a Nikon A1 eclipse Ti confocal microscope.
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bioRxiv - Molecular Biology 2022Quote: ... the membranes were incubated for 1 hour at room temperature with a goat anti-mouse alkaline phosphatase-conjugated secondary antibody (dilution 1: 5000; Sigma-Aldrich, USA) and developed in a solution of nitroblue tetrazolium chloride (NBT ...
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bioRxiv - Neuroscience 2022Quote: ... immunohistochemical examination of all surgical brain specimens was performed using the following panel of antibodies: mouse monoclonal anti-NeuN (clone A-60, Millipore Cat#MAB377), mouse monoclonal anti-GFAP (clone 6F2 ...
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bioRxiv - Neuroscience 2022Quote: ... mid-sagittal cerebellar vibratome sections were blocked and permeabilized with 10% normal goat serum in PBS containing 0.2% Triton X-100 and immunostained with a mouse anti-Calbindin D28k antibody (Sigma-Aldrich, 1:1000) followed by incubation with Alexa Fluor 555-labeled goat anti-mouse secondary antibody (Thermo Fisher ...
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bioRxiv - Microbiology 2024Quote: ... The V5 tagged proteins were detected in cell and virus lysates using anti-V5 mouse monoclonal antibody (Cat# V8012, Sigma, 1:1,000). Loading control for cell lysate was detected with anti-α-tubulin (rabbit polyclonal ...
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bioRxiv - Neuroscience 2024Quote: ... before being incubated overnight at 4°C with mouse anti-TH antibody (1:8000 dilution, T2928, Sigma-Aldrich, St. Louise, MO, USA), 1% NHS ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated o/n at 4°C with the primary antibodies (Rabbit-antiGFAP: MAB3402, Merck Millipore, 1:500; Mouse-antiNeuN: MAB377, Merck Millipore, 1:500) diluted in the blocking solution ...
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bioRxiv - Neuroscience 2024Quote: ... 75 μg of sample was incubated at 4°C overnight on rotator with 5 μg mouse anti-puromycin antibody (Millipore Sigma: MABE343), or rabbit anti-eIF4E (Abcam ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated with mouse monoclonal antibodies against NeuN protein (Anti-NeuN, clone A60; 1:20K dilution; Millipore, St. Louis, MO, USA) overnight at 4°C ...
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bioRxiv - Genetics 2024Quote: ... and secondary antibodies (anti-mouse HRP [1:2,000; Thermo Fisher Scientific] for TARS1 and anti-rabbit HRP [1:5,000; EMD Millipore] for actin) were applied in 2% milk solution ...
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bioRxiv - Immunology 2024Quote: ... The membranes that were loaded with fetal membrane and uterine tissue lysates were re-probed for 1 h at room temperature with a mouse anti-β-actin (ACTB) monoclonal antibody (Cat# A5441, Sigma-Aldrich). Then ...
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bioRxiv - Cell Biology 2023Quote: ... For immunolabeling of Cajal-Retzius like cells the following antibodies were used at a dilution of 1:200: Reelin (mouse, Sigma-Aldrich, MAB5366), and Mitochondria (mouse ...
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bioRxiv - Microbiology 2023Quote: ... Horseradish peroxidase-conjugated secondary antibodies (anti-mouse or anti-rabbit, at dilution 1:10000) and Immobilon® Western Chemiluminescent HRP substrate (Merk Millipore, Sigma Aldrich) were used to visualize FLAG-FlaA/FlaB and untagged FlaA/FlaB.
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bioRxiv - Molecular Biology 2023Quote: ... The cells on coverslips were blocked with blocking solution (2% BSA/PBST) for 30 min and incubated with the primary antibodies anti-Flag (mouse, Sigma, M2, F1804), anti-PABPN1 (rabbit ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies were diluted in 10% goat serum and incubated for 18 hours at 4°C with laminin rabbit anti-mouse antibody (1:250, Sigma-Aldrich, L9393). Sections were then incubated for 1 hour at room temperature in their corresponding goat anti-rabbit antibodies conjugated to Alexa Fluor 568 (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... The upper part of the membrane was incubated with a primary monoclonal mouse-anti-HA antibody (1:1000; H3663; Sigma-Aldrich, USA) to detect HA-tagged TRPM2 channel domain and the lower part of the membrane was incubated with rabbit-anti-FLAG (DYKDDDDK ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated overnight at 4 °C with BB containing a primary antibody cocktail (1:150 mouse anti-ionized calcium-binding adapter molecule 1 (Iba1; EMD-Millipore cat# MABN92+), 1:300 rabbit anti-transmembrane protein 119 (TMEM119 ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins of the Co-IP were analyzed by Western Blot with the Monoclonal ANTI-FALG® M2 antibody produced in mouse (Sigma Aldrich), proteins of inputs were analyzed by Western Blot with the Anti-GFP from mouse (Roche ...
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bioRxiv - Immunology 2023Quote: ... Sections were incubated for 48-72 hours with the following primary antibodies at 1:400: rabbit anti-Iba1 (Wako, 019-19741) and mouse anti-PSD-95 (Sigma-Aldrich, MAB1598). Sections were then washed 3x with PBS containing 0.05% Tween 20 and incubated with Alexa Fluorophore conjugated goat anti-rabbit IgG (H+L ...
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bioRxiv - Neuroscience 2023Quote: ... Protein was transferred to 0.45 µm nitrocellulose membranes prior to overnight incubation at 4°C with the following primary antibodies: mouse anti-ATXN3 (1H9; 1:1000, MAB5360; Millipore, Billerica, MA), mouse anti□GAPDH (1:10000 ...
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bioRxiv - Neuroscience 2023Quote: ... followed by a 16-h incubation in PBS with 0.1% Triton™ X-100 with the following antibodies: mouse monoclonal anti-NeuN (1:500; EMD Millipore, MAB377), rabbit polyclonal anti-KCC2 (1:1000 ...