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Citations for Millipore Sigma :
3801 - 3850 of 10000+ citations for Biotin Z Antibody Internalization Kit mouse since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... The grid was subsequently washed with MQ water followed by incubation for 20 min with 10 μl of anti-mouse 10 nm gold-labeled secondary antibody (1:10) (Sigma-Aldrich). Further ...
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bioRxiv - Microbiology 2023Quote: ... followed by incubation for 1 hour at room temperature with a horseradish peroxidase conjugated anti-mouse secondary antibody (Cat# NA931-1ML, Millipore Sigma) diluted 1:1000 in 3% milk ...
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bioRxiv - Neuroscience 2023Quote: ... every 4th section was immersed in 1% skim milk for 1 h at room temperature and incubated overnight at 4°C with mouse anti-FLAG monoclonal antibody (1:500; MAB3118, Sigma-Aldrich) or mouse anti-HA monoclonal antibody (1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... The fixed animals were washed sequentially in Borate-Triton buffer (with added βME) and then incubated at 4°C overnight in mouse anti-acetylated tubulin primary antibody (Ab) (Sigma T6793), at a dilution of 1:100 in 0.5% PBST ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were washed four times with TBST and incubated with secondary antibody (goat-anti-rabbit or donkey-anti-mouse HRP-conjugated,1:5000 in TBST + 10% dry milk, Sigma Aldrich) for 1 hour at RT ...
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bioRxiv - Bioengineering 2023Quote: ... The cells were then incubated overnight at 4°C in primary antibody solutions with anti-α-tubulin (mouse monoclonal, 1:1,000, T9026, Sigma-Aldrich) or anti-TOMM20 (Rabbit polyclonal ...
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bioRxiv - Cancer Biology 2023Quote: ... Immunoreactive bands were detected with horseradish peroxidase-coupled anti-mouse or anti-rabbit antibodies (CST) using the ECL System (Merck Millipore; WBKLS0500). Immunoblot analysis was performed using the LI-COR Odyssey Imaging System.
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bioRxiv - Cell Biology 2023Quote: ... and then incubation overnight at 4°C in blocking solution with primary antibodies: mouse anti-CRE (Sigma, F3165-2MG, 1:1000); mouse anti-Luciferase (Sigma ...
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bioRxiv - Genomics 2023Quote: ... and cells were resuspended in 100 μL dig-wash buffer containing 2 mM EDTA and a 1:50 dilution of rabbit anti-mouse antibody (Sigma, M7023) and incubated at RT for 1 hour with slow rotation ...
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bioRxiv - Microbiology 2023Quote: ... anti-p-PKC-δ-T505 (AP0776) followed by the respective secondary antibodies (horseradish peroxidase-conjugated anti-mouse or anti-rabbit IgG; Sigma). Mouse-monoclonal anti-β-actin-peroxidase antibody (A3854 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... This was followed by a 1 h incubation at room temperature with anti-mouse or anti-rabbit secondary antibodies conjugated with horseradish peroxidase (HRP; Sigma, A8924). Blots were developed using a Konica SRX101A X-Ray Film Processor.
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bioRxiv - Systems Biology 2023Quote: ... well-validated custom and commercial primary antibodies against proteins of interest were carefully selected and experimental parameters rigorously optimized to ensure high fidelity labeling: Connexin 43 (Cx43; mouse monoclonal antibody, Cat. # MAB3067, EMD Millipore Corp. ...
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bioRxiv - Molecular Biology 2023Quote: ... The FLAG-tagged protein was detected by incubating the milk powder-blocked membrane with mouse monoclonal anti-FLAG primary antibody (Sigma-Aldrich) diluted at 1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... we blocked membranes with 5% milk in PBS for 1 hour at room temperature and probed overnight with rocking at 4°C with the following primary antibodies: mouse anti-FLAG clone M2 (1/1000 dilution, Sigma # F1804), mouse anti-HA clone 16B12 (1/3000 dilution for HEK-293 cell lysates ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were washed with TTBS and incubated for 1 h at room temperature with isotype specific secondary antibodies anti-mouse IgG (1:10,000; Sigma-Aldrich, A4416) and anti-rabbit IgG (1:80,000 ...
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bioRxiv - Cancer Biology 2023Quote: ... The grid was subsequently washed with MQ water followed by incubation for 20 min with 10 μl of anti-mouse 10 nm gold-labelled secondary antibody (1:10) (Sigma-Aldrich) and 10 μl of anti-rabbit 5 nm gold-labelled secondary antibody (1:10 ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were then incubated for 48 hr at 4°C with mouse anti-TH antibody (1:2,000; Millipore, Burlington, MA) in 0.1 M PBS containing 2% normal donkey serum and 0.1% Triton X-100 ...
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bioRxiv - Genetics 2023Quote: ... or with a 1:4000 dilution of a mouse monoclonal anti-α-tubulin antibody (clone B 5-1-2) (Sigma Aldrich). The anti-Crym antibody is specific for an epitope that is located between amino acids 45 and 85 of the Crym protein ...
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bioRxiv - Biochemistry 2023Quote: ... Detection of 3x-FLAG tagged S was performed with 1:400 monoclonal ANTI-FLAG® M2 antibody [F3165] produced in mouse (Sigma). 1:50,000 Alexa Fluor® 680 AffiniPure Goat Anti-Mouse IgG [115-625-174] (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated with anti-calbindin-D28k mouse monoclonal antibody (1:2000 dilution by the blocking solution, C9848, Millipore Sigma) at 4 °C overnight ...
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bioRxiv - Neuroscience 2023Quote: ... Pelleted nuclei were resuspended in DPBS and incubated for 45 minutes with the mouse monoclonal antibody against neuronal nuclear marker NeuN conjugated to AlexaFluor 488 (1:1000, MAB377X; Millipore, MA). Before sorting ...
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bioRxiv - Biochemistry 2023Quote: ... After washing the membranes were probed with a 1:10,000 dilution of alkaline-phosphatase-conjugated goat anti-mouse IgG secondary antibody (Sigma-Aldrich, A2179) in PBST ...
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bioRxiv - Physiology 2023Quote: ... the secondary antibody was applied by incubating the slides for 1 hour at room temperature with blocking buffer containing Alexa Fluor 647-conjugated goat anti-mouse antibody (1:1000, Sigma, SAB4600354). The slides were then briefly rinsed in PBS-Tw before a 10-minute incubation in PBS-Tw containing 1:2000 SYTOX Orange (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... and 0.2% Triton X-100 diluted in PBS for 1 h before incubation with the following antibodies: mouse anti-GFAP (1:1,000; Millipore, Cat. MAB360), rabbit anti-LCN-2 (1:200 ...
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bioRxiv - Genetics 2023Quote: ... Primary antibody was anti-acetylated α-tubulin mouse monoclonal clone 6-11B-1 (1:500 dilution, Sigma, T7451-200, lot #077M4751V). Secondary antibody was Alexa Fluor 488 donkey anti-mouse IgG (1:1,000 dilution ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were electrophoresed on polyacrylamide gels transferred onto PVDF membranes and probed using the following antibodies: mouse anti-beta-Actin (Sigma #A1978), mouse anti-Vinculin (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... Tubulin labeling was carried out with a mouse monoclonal anti-acetylated tubulin antibody (T6793-6-11B-1, Sigma-Aldrich, 1:250) and followed by either a goat anti-mouse IgG Cy3 (115-165-166 ...
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bioRxiv - Cell Biology 2023Quote: ... The blot was blocked in 5% milk with TBS-0.1%Tween for 2 hours at room temperature on a rocker and then incubated with indicated primary antibodies overnight at 4°C (1:1000 mouse anti-degron (Sigma-Aldrich) or 1:5000 mouse anti-tubulin (ThermoFisher)) ...
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bioRxiv - Cell Biology 2023Quote: ... 2 % SDS and 100 mM β- mercaptoethanol] at 60 °C for 45 min and reprobed with mouse monoclonal anti-β-actin antibody (0.4 μg/ml; Sigma Aldrich) overnight at 4°C and HRP-conjugated goat anti-mouse IgG (0.2 μg/mL ...
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bioRxiv - Bioengineering 2023Quote: ... Hydrogels were then incubated overnight with primary antibodies against Yes-associated protein (YAP) (mouse monoclonal anti-YAP, 1:400, Sigma-Aldrich) at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... Membranes were washed three times with PBST for 5 minutes each before incubating with anti-mouse secondary antibodies (Sigma-Aldrich, F1804) for at least 60 minutes ...
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bioRxiv - Physiology 2024Quote: ... and proteins were detected by Western blot using monoclonal anti-Flag M2 antibody produced in mouse (1:5000 dilution; Sigma-Aldrich), rabbit anti-FKBP12 antibody (1:1000 dilution ...
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bioRxiv - Physiology 2024Quote: ... was incubated for 3 h at 4 °C with 1 μl of mouse anti-Flag M2 antibody or 3X Flag peptide (200 mg/ml; Sigma-Aldrich) on a rocking platform ...
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bioRxiv - Biophysics 2024Quote: ... isolated myofibrils were incubated for 1.5 hrs at 4°C on a rotator with 11 nM anti-α-actinin mouse antibody (A7811; Sigma-Aldrich), 5.5 nM Alexa-488 goat anti-mouse IgG (A11001 ...
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bioRxiv - Genomics 2024Quote: ... The following primary antibodies are used for the first target protein at the dilutions indicated: NeuN (1:500, A60, Mouse, Millipore MAB5374), pTDP43 (1:1000 ...
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bioRxiv - Developmental Biology 2024Quote: ... membranes were incubated for 2 hours at room temperature with monoclonal anti-β-actin HRP-conjugated mouse antibody (A3854, Sigma-Aldrich). Membranes were developed with Immobilon® Forte Western HRP Substrate (Cat ...
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bioRxiv - Biophysics 2024Quote: ... Immunostaining was performed as previously described [84] using the following antibodies: Mouse anti-Rac1 (Millipore Cat#05-389-25UG, 1:200), Rabbit anti-RhoA (Proteintech Cat#10749-1-AP ...
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bioRxiv - Developmental Biology 2024Quote: ... Primary antibodies used in this study were as follows: mouse anti-Actub (1:1,000 or 1:2,000, Sigma, 6-11B-1), guinea pig anti-Ick (1:100 ...
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bioRxiv - Cell Biology 2024Quote: ... with 200 μl of 15 μg/ml anti-CD63 antibody (Cosmo Bio, SHI-EXO-M02) or mouse IgG2a isotype control (Millipore, MABF1080Z) in PBS containing 0.02% Tween 20 ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated for 24 h at 4°C with a mixture of a monoclonal mouse anti-chondroitin sulfate antibody (CS-56, 1:500; Sigma-Aldrich) and a polyclonal rabbit anti-PV antibody (1:2000 ...
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bioRxiv - Immunology 2024Quote: ... After 3 x 5 min washes in deionized water unspecific binding sites were blocked with 0.5% fish skin gelatin (FSG) in PBS for 10 min before incubation with gold conjugated mouse IgM antibody (G5652, Sigma Aldrich) for 30 min ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were then rinsed 3 times for 10 minutes in PBS and incubated overnight at 4°C in a mix of the primary antibodies (mouse anti-S100β, Sigma Aldrich #S2532 ...
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bioRxiv - Microbiology 2024Quote: ... Organisms were stained with anti-MOMP (Meridian Biosciences) primary antibody for all the strains and primary mouse anti-Cpf1 (dCas12) (Sigma-Millipore) for knockdown or complementation samples ...
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bioRxiv - Cell Biology 2020Quote: ... 10 μl of a solution of BSA-biotin (1 mg/ml in ultra-pure water MQ, Sigma Aldrich, A8549) was incubated for 5 min after being washed with 50 μl washing buffer (1x PBS containing 10 mM MgCl2) ...
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bioRxiv - Cell Biology 2020Quote: ... 1 ml Fluorobrite containing 800 μM biotin and 1000 μg/ml L-ascorbic acid-2-phosphate (Sigma Aldrich, A92902) was added to the dish (final concentration 400 μM biotin and 500 μg/ml respectively ...
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bioRxiv - Molecular Biology 2020Quote: ... The beads were washed three times with NETN buffer and then eluted with 2 mg/ml biotin (Sigma-Aldrich) for 2 hr at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Unhooking of RUSH-TfR from the ER was allowed by the addition of D-biotin (Sigma-Aldrich, B4501-500MG) at 100 μM final concentration in DMEM for 30 min prior fixation ...
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bioRxiv - Cell Biology 2019Quote: ... and then immobilized on coverslips incubated sequentially with the following solutions: (1) 1 mg/ml BSA-biotin (A8549, Sigma), (2 ...
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bioRxiv - Neuroscience 2019Quote: ... Synchronous release of Nrxn1α or TfR was induced by application of 40 μM D-biotin (Sigma-Aldrich, Cat #B4501) 10 min after the beginning of the imaging session ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were incubated in one of three conditions for 24h: 1) 1μg/mL tetracycline (Bioshop) and 50 μM biotin (Sigma); (2 ...