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Citations for Millipore Sigma :
351 - 400 of 536 citations for Nuclease, Micrococcal since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Beads were resuspended in benzonase mix [500 units benzonase nuclease (E1014, Sigma-Aldrich) in NP-T buffer with 1 mM MgCl2] and incubated for 10 min at 37°C with shaking at 900 rpm ...
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bioRxiv - Biochemistry 2023Quote: ... Free genomic and plasmid DNA was digested with a Benzonase Nuclease (Sigma-Aldrich). Cell debris was removed by centrifugation and ...
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bioRxiv - Microbiology 2023Quote: ... The collected supernatants were incubated with 3 µl benzonase nuclease (Sigma-Aldrich, USA) overnight at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... 3 s on/17 s off) and treated with 1% Benzonase nuclease (Millipore). Lysates were clarified by centrifugation at 21,000 x g for 10 minutes at 4°C and quantified by Pierce 660 assay according to the manufacturer’s cuvette protocol ...
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bioRxiv - Microbiology 2024Quote: ... Cells were sonicated and Benzonase® Nuclease (Merck, Millipore Corp, Burlington, Massachusetts, USA) was added at a concentration of 1 U/ml ...
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bioRxiv - Biochemistry 2023Quote: ... the resulting tRNA was digested to single nucleotides using nuclease P1 (Sigma-Aldrich). Resulting 5’-32P labelled p*G or p*m1G were resolved by thin layer chromatography on cellulose plates in an isobutyric acid ...
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bioRxiv - Molecular Biology 2021Quote: ... The mRNAs samples were digested overnight with 0.1 U Nuclease P1 (Sigma-Aldrich, N8630) and 1.0 U calf intestinal phosphatase (NEB ...
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bioRxiv - Developmental Biology 2019Quote: ... fractions or total cell lysates in RIPA were treated with Benzonase nuclease (Sigma-Aldrich) to remove RNA and DNA and then incubated overnight with the appropriate antibody and then incubated overnight with a 50:50 mixture of protein A and protein G Dynabeads (Thermo Fisher Scientific) ...
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bioRxiv - Systems Biology 2021Quote: ... RM 13 KO cell line was generated by pre-designed zinc finger nucleases (Sigma) as described previously (50) ...
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bioRxiv - Immunology 2022Quote: ... 1 μL of virus lysate was treated with 250 U of benzonase nuclease (Sigma) at 37°C for 1 h ...
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bioRxiv - Molecular Biology 2020Quote: ... 5–10 ng of DNA template and nuclease-free water (Sigma, St. Louis, USA). We included both positive controls (DNA from L ...
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bioRxiv - Bioengineering 2021Quote: ... DNA-coated beads were diluted in nuclease-free water with 18 % OptiPrep (Sigma-Aldrich) to a final concentration of 1 to 2 beads per 14 pL droplet ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... the cell lysate was treated with 100 μg/mL DNA Nuclease I (Sigma Aldrich) and rotated at 4 °C or until no longer viscous ...
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bioRxiv - Developmental Biology 2021Quote: Zinc finger nuclease (ZFN) plasmids targeting the LEPTIN gene were designed by Sigma-Aldrich. Fetal fibroblasts were isolated from 30-day old male pig embryo ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 mM MgCl2) with 1% SDS and 1:2,000 Benzonase® nuclease (Sigma E1014). For tissue samples ...
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bioRxiv - Plant Biology 2022Quote: ... the plugs were incubated with 22µl of S1 nuclease (Sigma-Aldrich, catalog No. M5761) for 30min at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with Halt protease inhibitors and Benzonase nuclease (Sigma, final concentration 25 U/ml). GFP-tagged proteins were immunopurified with GFP-Trap magnetic beads (Chromotek ...
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bioRxiv - Biochemistry 2022Quote: ... followed by incubation for 30 min on ice with 5 µl benzonase nuclease (Sigma). The lysates were then centrifuged for 20 min at 45,000 g and 4 °C and the supernatants were filtered (0.45 μM ...
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bioRxiv - Immunology 2022Quote: ... supplemented with 10% fetal bovine serum (FBS) and 0.05 U/mL benzonase nuclease (Millipore). The cells were initially stained and incubated with LIVE/DEAD™ Fixable Blue Dead Cell Stain Kit (ThermoFisher ...
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bioRxiv - Cell Biology 2023Quote: ... and 0.1 µl/ml Benzonase nuclease (cat#70746-10KUN, Merck Millipore, Burlington, MA, USA) by passing the cell suspension through a G25 needle ten times ...
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bioRxiv - Pathology 2023Quote: ... samples were homogenized and then incubated with 25 units/ml nuclease (benzonaseTM, Sigma-Aldrich) and 50 mM MgCl2 for 30 minutes at 37 °C at 120 x g followed by a digestion with 1 mg/ml PK (final concentration ...
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bioRxiv - Molecular Biology 2023Quote: ... 1mM MgCl2 and Benzonase Nuclease 250 units/ 10 millions of cells (E1014-25KU, Sigma). Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 mM MgCl2) with 1% SDS and 1:2,000 Benzonase® nuclease (Sigma E1014). Cell debris was removed by centrifugation at 16,000x g for 5 minutes at room temperature and protein concentration was measured using A280 values determined by Cytation 5 (BioTek) ...
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bioRxiv - Molecular Biology 2023Quote: ... The solution of siRNA (20 pMol) in 2.5 µL nuclease-free water (Sigma, USA) was mixed with 2.5 µL of peptide’s solutions in nuclease-free water ...
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bioRxiv - Synthetic Biology 2021Quote: ... The final purified ssDNA sample was eluted with nuclease-free water (Sigma Aldrich, Cat. # W4502) from the silica column of an EndoFree® Plasmid Maxi Kit ...
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bioRxiv - Cell Biology 2020Quote: ... Pellet fraction was incubated with 1.25 U/μl of benzonase nuclease (Merck, Millipore, Burlington, MA) on ice for 10 min prior to denaturation in 4X loading buffer (Li-Cor).
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bioRxiv - Developmental Biology 2020Quote: ... Protein lysis was incubated on ice with 50 U of Benzonase Nuclease (Millipore, #E1014-5KU) for 20 min and at 37 °C for 5 min ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 μg of total RNA was digested with 2 U of Nuclease P1 (Sigma-Aldrich) in 20 μL of 10 mM Tris (pH 7.0) ...
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bioRxiv - Synthetic Biology 2019Quote: ... After addition of 10 mM MgCl2 and treatment with 125 U Benzonase Nuclease (Sigma-Aldrich) for 10 min ...
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bioRxiv - Immunology 2021Quote: ... were treated with 250 units (U) of Benzonase Nuclease (Sigma-Aldrich Chemie GmbH, Munich Germany) for 1 h at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... The pellets were resuspended and lysed in RIPA buffer supplemented with benzonase nuclease (Merck Millipore). Lysates were centrifuged again ...
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bioRxiv - Cell Biology 2020Quote: ... total cell lysates were collected in Laemmli buffer and treated with benzonase nuclease (Millipore-Sigma). Proteins were separated on SDS–PAGE gels and transferred to PVDF membrane (Amersham Hybond P ...
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bioRxiv - Cell Biology 2020Quote: ... total cell lysates were collected in Laemmli buffer and treated with benzonase nuclease (Millipore-Sigma). Proteins were separated on SDS–PAGE gels and transferred to PVDF membrane (Amersham Hybond P ...
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bioRxiv - Microbiology 2021Quote: ... protein extracts were incubated for 45 min with 1%v/v Benzonase Nuclease (SIGMA E1014). For the FASP digestion ...
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bioRxiv - Cell Biology 2019Quote: ... Reactions were stopped by treatment with SDS-PAGE sample buffer containing benzonase nuclease (Sigma, E1014) and boiling for 5 min ...
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bioRxiv - Bioengineering 2020Quote: ... the glass fiber capture membrane was washed three times in nuclease free water (Sigma-Aldrich) and dried overnight at room temperature ...
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bioRxiv - Immunology 2020Quote: 50 μL of viral lysate was treated with 250 U of Benzonase® Nuclease (Sigma) in 250 μM MgCl2 at 37°C for 1 h ...
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bioRxiv - Microbiology 2020Quote: ... The remaining 8/10th of beads were digested with 100 units of Benzonase nuclease (Millipore) at 37°C for 1 hour ...
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bioRxiv - Cell Biology 2019Quote: ... The pellet was subsequently lysed in lysis buffer containing 5 units/uL benzonase nuclease (Sigma) for 1 h and centrifuged at maximum speed for 5 min ...
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bioRxiv - Cancer Biology 2019Quote: Single cell suspensions prepared as above were treated with benzonase nuclease (Sigma #E1014, 1:10,000), stained with cisplatin ...
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bioRxiv - Plant Biology 2022Quote: ... followed by three washes with 250µl of S1 nuclease buffer (Sigma-Aldrich, catalog No. M5761), for 45min each wash ...
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bioRxiv - Molecular Biology 2023Quote: ... A final solution of 0.5% sodium deoxycholate and 50 U/mL of benzonase nuclease (Sigma) was added to the solution ...
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bioRxiv - Neuroscience 2023Quote: ... The lysate and the PEG-precipitate was treated with benzonase nuclease HC (#71206-3, Millipore) for 45 minutes at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... Then each cell lysate was treated with 100 units of benzonase nuclease (Millipore Sigma, E1014) and incubated at 4°C with shaking for an hour ...
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bioRxiv - Neuroscience 2024Quote: ... and 0.5% Sodium deoxycholate and incubated with 100 U/mL Benzonase nuclease (Sigma #E1014 25KU) for 1 h to dissociate particles from membranes ...
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Somatostatin interneurons control the timing of developmental desynchronization in cortical networksbioRxiv - Neuroscience 2023Quote: ... and 0.5% Sodium deoxycholate and incubated with 100 U/ml Benzonase nuclease (Sigma, E1014 25KU) for 1 hr ...
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bioRxiv - Immunology 2021Quote: ... After thawing in RPMI supplemented with 10% FBS and benzonase nuclease (Millipore EMD 0.05 U/ml), the samples were incubated with LIVE/DEAD™ Fixable Blue Dead Cell Stain Kit and the Human BD Fc Block for 30 min at room temperature before being simultaneously stained with four phenotyping panels that have been previously described in OMIP-044 and OMIP-064 and further described in Table S2 (Hertoghs et al. ...
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bioRxiv - Bioengineering 2022Quote: ... and 4 μL of nuclease-free water in each well on the 1.2% agarose gel (Sigma) stained with Ethidium Bromide (1 mg/μL ...
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bioRxiv - Bioengineering 2021Quote: ... One to four μg of tRNA was digested with 1 unit of nuclease P1 (Sigma Aldrich) in a 10 μL solution of 10 mM NH4OAc at 45°C for 2 hrs ...
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bioRxiv - Microbiology 2021Quote: ... the cell suspension obtained 2.5h post-nuclease treatment were washed thrice in PBS,pH 7.2 (Sigma) and centrifuged each time at 4000 × g for 15 min (Microfuge 20R ...