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Citations for Millipore Sigma :
1 - 50 of 536 citations for Nuclease, Micrococcal since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... Micrococcal Nuclease digestion was carried out by adding 0.1U Micrococcal Nuclease (Sigma) per 100ul buffer and incubating for 1 min 40 sec in a 37°C water bath ...
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bioRxiv - Biochemistry 2020Quote: ... Micrococcal nuclease (Sigma, #N3755) (0.5 U/µl in 50 mM Tris ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Micrococcal Nuclease (MNase, Sigma N3755) was reconstituted to a concentration of 1 unit per 5 μl with nuclease-free water ...
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bioRxiv - Biochemistry 2023Quote: ... Micrococcal Nuclease (MNase) (Sigma N5386) was diluted to 0.5 U/µl in MNase dilution Buffer (50% Glycerol ...
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bioRxiv - Genomics 2020Quote: ... micrococcal nuclease from Staphylococcus aureus (Sigma, reconstituted in ddH2O ...
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bioRxiv - Cancer Biology 2020Quote: ... PBS supplemented with 1U/ml micrococcal nuclease (Sigma-Aldrich) was then added to the neutrophils for 10min at 37°C and 5% CO2 to detach the NETs ...
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bioRxiv - Cancer Biology 2020Quote: ... neutrophils were treated with 1U/ml micrococcal nuclease (Sigma-Aldrich) for 10min (found to be the optimum time for detaching NETs from neutrophils without digesting the NETting DNA ...
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bioRxiv - Molecular Biology 2023Quote: ... After adding 30 units of micrococcal nuclease (Sigma-Aldrich N3755), the lysate was incubated at 24°C ...
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bioRxiv - Developmental Biology 2023Quote: ... nuclei were extracted and digested with micrococcal nuclease (MNase, Sigma). A population of mono-and di-nucleosomes were used in chromatin immunoprecipitation assays ...
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bioRxiv - Biochemistry 2021Quote: ... and treated with 0.2 U Nuclease S7 Micrococcal nuclease from Staphylococcus aureus (Sigma-Aldrich, 10107921001) as previously described (O’Keefe et al. ...
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bioRxiv - Microbiology 2022Quote: ... RNase I was replaced with 7 µg of micrococcal nuclease (Sigma) as described above.
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bioRxiv - Biochemistry 2022Quote: ... Nucleic acids were digested with 15 units micrococcal nuclease (Sigma Aldrich) for 20 min at 37°C ...
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bioRxiv - Plant Biology 2019Quote: ... The nuclei were then digested with micrococcal nuclease (MNase, Sigma #N5386-500UN). Following digestion a portion of the chromatin was set aside as the input control sample ...
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bioRxiv - Molecular Biology 2019Quote: ... Chromatin was solubilized by incubation with 100 units of Micrococcal nuclease (Sigma, N3755) for 20 minutes at 37° C ...
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bioRxiv - Plant Biology 2021Quote: ... nuclei were extracted and mononucleosomes were generated with micrococcal nuclease (MNase) (Sigma, N5386) digestion as previously described (Jiang and Berger ...
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bioRxiv - Molecular Biology 2023Quote: ... Chromatin digestion was performed by adding 0.5 mM CaCl2 and micrococcal nuclease (Sigma) at a concentration of 1U/50 µg of DNA at 37°C ...
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bioRxiv - Pathology 2023Quote: ... Filtrates were treated with a cocktail of 2.0 μL of micrococcal nuclease and 1.0 μL benzonase (2,000,000 gel units/mL Micrococcal nuclease, NEB Inc, and >250 units/μL Benzonase™nuclease, Millipore Sigma) in 7.0 μL of resolving enzyme buffer to remove any free nucleic acids before performing the nucleic acid extraction (41) ...
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bioRxiv - Molecular Biology 2019Quote: ... Chromatin was solubilized by incubation with 4-20 units of Micrococcal nuclease (N3755, Sigma) for 10 minutes at 37° C ...
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bioRxiv - Biochemistry 2021Quote: ... resuspended in 100 ul KHM buffer and endogenous mRNA removed by treatment with 0.2 U Nuclease S7 Micrococcal nuclease from Staphylococcus aureus (Sigma-Aldrich, 10107921001) in the presence of 1 mM CaCl2 at 22°C for 12 min ...
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bioRxiv - Molecular Biology 2022Quote: ... Spheroplasted cells were treated with 0.01171875 or 0.1875 U micrococcal nuclease (Sigma-Aldrich, N5386-200UN) in digestion buffer (1 M sorbitol ...
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bioRxiv - Molecular Biology 2024Quote: Nuclear chromatin was digested with either 8 or 16 units of micrococcal nuclease (MNase) (Sigma-Aldrich) in 150 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... The insoluble chromatin pellets were then solubilized in EBC2 buffer supplemented with micrococcal nuclease (Sigma, cat # N3755) by digesting for 45 min at 30°C ...
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bioRxiv - Bioengineering 2020Quote: ... and 300 µL aliquots of suspension were treated with 0.4 U micrococcal nuclease (MNase, Sigma ; Art. No.: N3755) at 37 °C for 6 min ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were digested with 1.25 μl of micrococcal nuclease (Mnase) (0.5 U/μl; Sigma-Aldrich; Cat#: N3755-500UN) for 5 min at 37 °C with 600 rpm ...
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bioRxiv - Biophysics 2021Quote: ... was generated from 1.2 ml of extract that was incubated with 0.5 U/ml of micrococcal nuclease (Sigma) at 25 °C for 5 min ...
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bioRxiv - Molecular Biology 2022Quote: ... the pellet was resuspended in 900 μl of MNB buffer and 180 μl aliquots were digested with different amounts of Micrococcal Nuclease (N3755, Sigma) (0 ...
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bioRxiv - Microbiology 2022Quote: ... 5 A260 units of the cleared cell lysates were incubated either for 30 min at RT either with 3 µg of micrococcal nuclease (Sigma), or 300 units of RNase T1 (Fermentas ...
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bioRxiv - Microbiology 2021Quote: ... was set aside for RNA-seq analysis while 200 μL was digested with 300 U/mL micrococcal nuclease S7 (MNase; Sigma Aldrich) for 30 mins at 37°C to generate ribosome-protected fragments (RPFs) ...
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bioRxiv - Microbiology 2023Quote: ... Samples were clarified by centrifugation and 200 µL of supernatant was treated with 1000 U of micrococcal nuclease (MNase) (Sigma-Aldrich) supplemented with a final concentration of 10 µM CaCl2 and incubated at 25°C shaking at 250 rpm for 1 h ...
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bioRxiv - Cancer Biology 2023Quote: The cytosol and CMX from control and treated cells were obtained as previously described and digested with micrococcal nuclease (MNase, Sigma-Aldrich, St Louis, MO) at 37°C for 30 min to obtain ribosome footprints ...
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bioRxiv - Molecular Biology 2020Quote: ... Benzonase nuclease (Sigma), and 10 nM iodoacetamide to prevent disulphide-bond formation during lysis ...
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bioRxiv - Cell Biology 2021Quote: ... Benzonase nuclease (Sigma) was added at the amount of 3 units per ml of original culture volume processed ...
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bioRxiv - Biochemistry 2020Quote: ... and Benzonase Nuclease (Sigma). Cells were lysed on ice by extrusion in a tissue homogenizer and lysates were cleared by ultracentrifugation at 25000 rpm for 45 minutes at 4°C using a Beckman Ti45 rotor ...
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bioRxiv - Biochemistry 2020Quote: ... and Benzonase Nuclease (Sigma). Cells were lysed on ice by extrusion in a tissue homogenizer and lysates were cleared by ultracentrifugation at 25000 rpm for 45 minutes at 4°C in a Beckman Ti45 rotor ...
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bioRxiv - Microbiology 2019Quote: ... Benzonase nuclease (Sigma-Aldrich) was added to lysis buffer to a concentration of 5 μl/ml to prevent nonspecific interactions mediated by RNA or DNA.
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bioRxiv - Cell Biology 2021Quote: ... Benzonase nuclease (Millipore E1014) was added to samples and incubated at room temperature for five minutes ...
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bioRxiv - Bioengineering 2021Quote: ... Nuclease P1 (Sigma Aldrich), Antarctic phosphatase (5,000 units/mL ...
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bioRxiv - Systems Biology 2022Quote: ... Benzonase Nuclease (Millipore, E1014) was added to 0.3 units/µL ...
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bioRxiv - Microbiology 2022Quote: ... and Benzonase nuclease (Millipore) for 1 hour at room temperature on a rocking platform ...
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bioRxiv - Microbiology 2019Quote: ... Salt-Active Nuclease (Sigma) was used to remove the gDNA according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2020Quote: ... Benzonase nuclease (EMD Millipore) was added (2μl) ...
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bioRxiv - Biochemistry 2020Quote: ... Benzonase nuclease (Novagen #70746) and EDTA-free protease inhibitor cocktail (Roche #04693159001).
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bioRxiv - Cell Biology 2019Quote: ... Microccocal nuclease (Sigma-Aldrich) was added to samples to the final concentration of 5 U/ml and ...
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bioRxiv - Immunology 2019Quote: ... and Benzonase Nuclease (Sigma). Primary antibodies for IFITM3 (Abcam ...
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bioRxiv - Plant Biology 2020Quote: Nuclease-free water (Sigma) was used for blank control treatment and dilution of all dsRNAs ...
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bioRxiv - Molecular Biology 2022Quote: ... and Benzonase nuclease (Millipore). Cell lysis was performed for 30 minutes at 4°C under gentle rocking.
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bioRxiv - Genomics 2023Quote: ... Benzonase Nuclease (Millipore E1014) was added to Papain (Millipore P4762 ...
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bioRxiv - Immunology 2023Quote: ... and benzonase nuclease (Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... with Benzonase nuclease (Millipore) and incubated at 37 °C for 30 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... and Benzonase Nuclease (Sigma) for 30 min on ice and clarified by centrifugation at maximum speed.