Labshake search
Citations for Millipore Sigma :
351 - 400 of 4041 citations for Interleukin 35 IL 35 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2023Quote: THP1 cells (0.5 million cells in 1ml) were seeded in a 35 mm dish and treated with PMA (10 ng/ml) (Sigma-Aldrich) for 24 hrs in RPMI complete media (+ 10% FBS ...
-
bioRxiv - Cell Biology 2024Quote: Cells cultured in each 35 mm dish were dissolved in 200 – 300 μL RIPA buffer (Thermo 89900) + 2 mM PMSF (Sigma P7626) + 3 μL Protease Inhibitor Cocktail (PIC ...
-
bioRxiv - Neuroscience 2021Quote: Human embryonic kidney (HEK293) cells were maintained in a 37°C/5% CO2 atmosphere in DMEM:F12 (Sigma, D6421) supplemented with 10% foetal bovine serum (ClonTech ...
-
bioRxiv - Neuroscience 2021Quote: ... MaxiSorp plates (Thermo) were coated with 100µl of goat anti-human Fc antibody (Sigma) diluted 1:500 in PBS overnight at 4°C ...
-
bioRxiv - Neuroscience 2021Quote: ... Plates were washed before detection and goat anti-human Fc-HRP conjugated antibody (Sigma) (1:5000 dilution in PBS ...
-
bioRxiv - Neuroscience 2020Quote: MaxiSorp plates were coated with 100 µl of goat anti-human Fc antibody (Sigma) diluted 1:500 in PBS or 3 mg/ml of an anti-VNAR antibody (courtesy of Martin Flajnik ...
-
bioRxiv - Microbiology 2023Quote: ... 10 μg/mL goat anti-human IgG1 Fc F(ab’)2 (Sigma-Aldrich, I3391) was coated in 96-well NUNC Immunoplate (Thermo Fisher ...
-
bioRxiv - Physiology 2023Quote: Rm-EphrinA4/Fc chimera (R&D) was clustered with anti-human Cy3 antibody (Sigma) diluted 1 ...
-
bioRxiv - Immunology 2022Quote: ... and further purified via size exclusion chromatography (SEC) on a HiLoad 16/600 Superdex 200 pg column (Millipore Sigma, 28-9893-35) with 10 mM Tris ...
-
bioRxiv - Neuroscience 2020Quote: ... and FoxP2-Cre mice using glass capillary (tip diameter 35 μm; 1-5 μL Hirschmann® microcapillary pipette, CAT#Z611239, Sigma-Aldrich) connected to a Picospritzer® pressure system (Parker Hannifin) ...
-
bioRxiv - Neuroscience 2019Quote: ... At this point we modified the protocol to incubate the samples in tert-Butanol incubation for 12 hrs at 35 °C followed by immersion in dichloromethane (Sigma-Aldrich, 270997) for 12 hrs at room temperature and finally incubation with the refractive index matching solution BABB (benzyl alcohol + benzyl benzoate 1:2 ...
-
bioRxiv - Biophysics 2020Quote: ... Concentrated cells in 5 μL were mounted on a thin layer formed from 35 μL of 25% gelatin (Sigma-Aldrich; G-2500) in EMM5S or other buffers (without the DTT) ...
-
bioRxiv - Bioengineering 2021Quote: ... EVs were collected by a size-based EV isolation method [35, 36] using 50 nm membrane filter (Whatman, WHA110603) with holders (EMD Millipore, SX0002500). Holders with 50 nm membrane filters were connected to a vacuum manifold (Qiagen ...
-
bioRxiv - Immunology 2020Quote: IgG mediated antibody titer of serum samples collected from immunized animals and convalescent patients’ sera were determined by enzyme-linked immunosorbent assay (ELISA)35 using 1:5000 dilution of HRP conjugated anti mouse (A4416, Sigma-Aldrich, USA) and 1:10000 dilution of HRP conjugated anti human secondary antibody (A0170 ...
-
bioRxiv - Immunology 2022Quote: ... we used a synthetic cathepsin C inhibitor (IcatCXPZ-01; (37) diluted in 10 % (2-Hydroxypropyl)-β-cyclodextrin (CAS: 128446-35-5; C0926; Sigma-Aldrich) suspended in citrate buffer 50 mM at pH=5 (vehicle ...
-
bioRxiv - Neuroscience 2022Quote: ... −1.8 mm from cortical surface) using glass microcapillary (tip diameter ≃35 μm; 1–5 μL Hirschmann® microcapillary pipette, Z611239, Sigma-Aldrich) connected to a custom Openspritzer pressure system (Forman et al. ...
-
bioRxiv - Microbiology 2023Quote: ... low phosphorus concentration of 35 mM (4.8 mg l1 KH2PO4) and 0.3% PhytagelTM for stabilisation (Sigma Chemical Co., St. Louis, MO, USA).
-
bioRxiv - Cell Biology 2023Quote: ... RNA was introduced by electroporation at stages 26-28 and RGC explants were taken at stages 35-36 and cultured for 19-24 h in 60% L-15 (Sigma-Aldrich, L1518) in water ...
-
bioRxiv - Cell Biology 2023Quote: ... were transferred to 35 mm diameter petri dishes containing 3 ml of liquid GM supplemented with 10 μM estradiol (β-Estradiol, E8875, Sigma Aldrich, freshly prepared by diluting a 1 mM stock solution in DMSO ...
-
bioRxiv - Biochemistry 2023Quote: Tardigrade proteins were extracted using a modified acid extraction.35 Tardigrades were incubated in concentrated trifluoroacetic acid (Sigma Aldrich, St. Louis, MO) for 40 min before neutralizing to approximately pH 7 with 2 M Tris (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2023Quote: ... supplemented with 2% charcoal-stripped FBS and EPC cocktail (1 µM MPA, Sigma Cat#1378001- 200MG, 0.05 mM cAMP, Axxora Cat #JBS-NU-1502-50, and 35 nM E2, Sigma Cat #E1024-1G).
-
bioRxiv - Cell Biology 2024Quote: ... the cells were cultured for a period of 48 hours on 35-mm-diameter culture dishes coated with 0.1 mg/ml poly-D-lysine (Sigma Aldrich, P0899-50MG). Subsequently ...
-
bioRxiv - Cell Biology 2023Quote: ... HeLa cells were plated at 10% confluency on an Ibidi Glass Bottom Dish 35 mm (Martinsried, Germany) and treated with 2 mM thymidine (Sigma, T1895-1G) for 18 hours to induce the first block ...
-
bioRxiv - Biophysics 2024Quote: ... 20 µL volumes of protein were plated on 35-mm dishes precoated with 1% Pluronic F-127 (Sigma Aldrich, St. Louis, Missouri). Subsequently ...
-
bioRxiv - Biochemistry 2021Quote: HEK293 and HEK293T cells (Sigma) were cultured at 37 °C ...
-
bioRxiv - Microbiology 2020Quote: The human embryonic kidney cell lines HEK293 and HEK293T were cultured in Dulbecco’s modified Eagle’s medium (D1152, Sigma-Aldrich) supplemented with 10% FBS ...
-
bioRxiv - Cell Biology 2023Quote: U2OS osteosarcoma cells and HEK293 (human embryonic kidney) cells were grown in DMEM (Dulbecco’s Modified Eagle’s Medium; Sigma, D1152) supplemented with 10% fetal bovine serum (FCS ...
-
bioRxiv - Molecular Biology 2020Quote: ... and anti-Human IgG (Fc specific) −Peroxidase antibody produced in goat was from Sigma-Aldrich. Two TNFα-antagonists were purchased ...
-
bioRxiv - Cell Biology 2022Quote: ... 100 μL of goat anti-human IgG (Fc specific)-Peroxidase antibody (1 : 5000 dilution, Sigma) was added and incubated for another 1 h at 37 °C ...
-
bioRxiv - Biophysics 2023Quote: ... The anti-human Fc capturing mAb (GG-7) was from Sigma-Aldrich (St. Louis, MO). LIBS-2 was purchased from EMD Millipore (Billerica ...
-
bioRxiv - Bioengineering 2023Quote: ... 100 µL of anti-human IgG (Fc specific)-peroxidase antibody produced in goat (Sigma, A0170) was added to each sample in a 1:40,000 dilution of PBS and incubated for 1 h ...
-
bioRxiv - Genetics 2021Quote: ... The probes (50 ng of Rsp and 35 ng of 359 bp probes per slide) were diluted in hybridization buffer (1mg/mL sonicated salmon sperm DNA (Sigma-Aldrich ref# D9156), 50% formamide ...
-
bioRxiv - Immunology 2020Quote: ... The Melgisorb wound dressing was submerged in 35 μL of Alginate Lyase in H2O (1 unit/mL concentration; derived from Flavobacterium, Sigma-Aldrich A1603-100MG), vortexed ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were incubated for 24h before monitoring cell viability using the MTT assay: cells were incubated at 35°C for 1 h with MTT (Sigma, Cat #M2128-10G). For that purpose ...
-
bioRxiv - Biophysics 2022Quote: ... were cultivated to 85% confluency in 35 mm culture dishes and transfected with 1.0 μg plasmid DNA using polyethyleneimine (Sigma, St. Louis, MO, USA). After 12 h at 37 °C in 5% CO2 ...
-
bioRxiv - Cell Biology 2023Quote: ... mice were fasted for 4 to 5 hours prior to an intravenous injection of a mixture of 200 μCi 35S-methionine and 500 mg/kg tyloxapol (Sigma-Aldrich, T8761-50G) in 0.9% NaCl ...
-
bioRxiv - Plant Biology 2023Quote: ... The seeds were placed in a 50 ml tube containing 35 ml of H2O and 30 mM EMS (Sigma-Aldrich, St. Louis, USA). The EMS was dissolved before by shaking for 5 mins ...
-
bioRxiv - Neuroscience 2023Quote: ... mice were administered eight sub-convulsive doses of the GABAAR antagonist PTZ (i.p., 35 mg/kg of PTZ, Sigma-Aldrich, St Louis, MO) or vehicle (NaCl 0.9% ...
-
bioRxiv - Microbiology 2024Quote: ... P3HR-1 ZHT/RHT or Akata cells lytic induction was triggered by addition of 400 nM 4-hydroxy tamoxifen (4-HT) (Sigma #68392-35-8) for 24 h or by 15 µg/ml α-human IgG (Agilent #A042301-2 ...
-
bioRxiv - Microbiology 2023Quote: ... Binding of VHH-Fcs or conventional human monoclonal antibodies was detected by HRP-conjugated rabbit anti-human IgG (Sigma, A8792, 1/2000). After washing 50 μL of TMB substrate (Tetramethylbenzidine ...
-
bioRxiv - Immunology 2022Quote: ... Antibody production was conducted by co-transfection of the heavy and light chain expression vectors into Human HEK293 suspension culture cells (Expi293F) using polyethylene mine (Sigma). Cells were subsequently cultured for 4 days before being pelleted by centrifugation ...
-
bioRxiv - Microbiology 2021Quote: ... and 20 ng/mL of recombinant human IL-13 (Sigma-Aldrich, St. Louis, MO) to promote differentiation into M2 macrophages ...
-
bioRxiv - Neuroscience 2021Quote: Quantification of IL-6 in media was assessed via Human IL-6 ELISA kit according to manufacturer instructions (Sigma Aldrich; St. Louis, MO).
-
bioRxiv - Microbiology 2022Quote: ... HEK293 WT (Sigma Cat. Nr. 85120602), HEK293 GALNT1 KO (Narimatsu et al. ...
-
bioRxiv - Immunology 2021Quote: ... HEK293 cells (Sigma Aldrich 12022001-1VL) were maintained in DMEM supplemented with L-glutamine and 10% FBS ...
-
bioRxiv - Developmental Biology 2021Quote: ... the cell is immersed in culture media that has been made denser by the addition of 35% OptiPrep (Sigma-Aldrich cat. no. D1556-250ML). The cell is then flow back through the cantilever in the high-density media to obtain a second set of buoyant mass and media density measurements for the cell ...
-
bioRxiv - Neuroscience 2021Quote: ... at a density of 40,000 cells/well in 24-well plates for EdU incorporation assays or on 35-mm tissue culture dishes coated with poly-L-lysine (Sigma, P1524; 30 μg/mL) at a density 10×106 cells/dish for immunoblotting ...
-
bioRxiv - Physiology 2019Quote: ... 6 dpf larvae in 35 mm Petri dishes were first incubated in 5 mL of steady state E2 medium + 100 µL of Tricaine (Sigma-Aldrich #E10521, Schnelldorf, Germany); they were considered to be anesthetized when they failed to respond to tactile stimulation ...
-
bioRxiv - Biochemistry 2020Quote: ... Taurocholic acid sodium salt (97% pure) and estrone-3-sulfate sodium salt (containing 35% Tris stabilizer) were purchased from Sigma Aldrich (St. Louis, MO). Rosuvastatin (98% pure ...
-
bioRxiv - Cell Biology 2023Quote: ... Neurons were isolated and maintained for 5-12 DIV on 35 mm coverslips (3.5×105 cells/plate) coated with poly-L-lysine/laminin matrix (Sigma-Aldrich, St. Louis, MO, USA). Primary neurons were grown on Neurobasal medium (Gibco BRL ...