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Citations for Millipore Sigma :
301 - 350 of 4041 citations for Interleukin 35 IL 35 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: Cells were fixed in 70% ethanol for at least 2 hrs at −20°C and resuspended in a PBS solution containing 35 ug/ml propidium iodide (Sigma) and 100 ug/ml RNAse A (Roche) ...
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bioRxiv - Biochemistry 2022Quote: ... 50 μg ml-1 spectinomycin (Sp) or/and 35 μg ml-1 chloramphenicol (Cm) or 100 μg·ml-1 ampicillin (Sigma, Merk).
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bioRxiv - Biochemistry 2022Quote: ... a total of 0.4 μg of plasmid and dsRNAs were transfected into BmN4 cells (4–6 × 104 cells per glass bottom 35 mm dish) with X-tremeGENE HP DNA Transfection Reagent (Sigma) and cells were fixed 4 days later ...
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bioRxiv - Physiology 2023Quote: ... animals from each group were labeled with an intraperitoneal injection of 100% 2H2O saline (35 µl/g body weight ∼3.5 % of body weight, deuterium oxide, Sigma 151890) and provided with 8% 2H2O drinking water for the remainder of the study to maintain body 2H2O enrichments of approximately 5% ...
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bioRxiv - Molecular Biology 2022Quote: ... The beads-antibody-protein mix was incubated overnight at 4°C and bounds protein were eluted with 35 μg HA peptides diluted in PBS (Sigma); then Laemmli buffer was added and the eluate heated at 95°C 2 min.
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bioRxiv - Evolutionary Biology 2022Quote: ... LC-ESI-MS analysis was carried out at 35 °C by loading 1 μg of the sample in 0.1% formic acid (Sigma-Aldrich) onto a 50-cm μPAC C18 column (Pharma Fluidics ...
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bioRxiv - Biochemistry 2023Quote: ... Cytiva) was used for affinity chromatography and a HiLoadTM 16/600 SuperdexTM 200 pg (cat# GE28-9893-35, Sigma Aldrich) for size exclusion chromatography (SEC) ...
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bioRxiv - Neuroscience 2023Quote: ... Adult male mice of the NOD-SCID strain (∼30–35 g) were administered 2 µl of 6-hydroxydopamine (6-OHDA, Sigma) dissolved in PBS at a concentration of 3.75 µg/µl with the addition of 0.01% ascorbic acid at the rate of 0.5 µl/min during a stereotaxic surgery ...
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bioRxiv - Systems Biology 2023Quote: ... Metabolite identification was verified by exact mass (to 35 ppm) and co-elution with authentic standards purchased from Millipore Sigma. Batch feature extraction and chromatographic alignment across multiple data files was performed using the Agilent MassHunter Profinder software and extracted metabolite data was exported for further statistical analysis using R ...
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bioRxiv - Neuroscience 2023Quote: ... the cell suspension was diluted in 2.5 ml of culture medium and plated in 35 mm culture dish previously coated for at least 2 hours with 0.1 mg/ml poly-D-lysine (Sigma-Aldrich) in the incubator ...
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bioRxiv - Microbiology 2023Quote: The pneumococcal capsule thickness was determined by measuring the zone of exclusion of FITC-dextran based on a modification of the method of Gates et al (35) using FITC-dextran (2000 kDa, Sigma).
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bioRxiv - Physiology 2023Quote: HEK293T cells were seeded on day one into 35 mm glass-bottom dishes (Matek) pre-coated with Poly-L-lysine (Sigma). On day three ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Seeding solutions were prepared by the addition of the appropriate volumes of sterile 35% BSA in DPBS (Sigma, cat # A7979) or DPBS to generate seeding solutions containing 0 ...
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bioRxiv - Microbiology 2023Quote: ... After a 5 h incubation, the medium was changed to infection medium (DMEM containing Pen/Strep, 35% Bovine Serum Albumin (BSA) (Sigma), 5% NaHCO3 ...
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bioRxiv - Neuroscience 2024Quote: ... using a subcutaneous injection of 200mg of MOG peptide (Myelin Oligodendrocyte Glycoprotein Peptide Fragment 35 to 55; from Charité) emulsified in complete Freund’s adjuvant (from Sigma-Aldrich) that contained 200mg of Mycobacterium tuberculosis H37RA (from Difco) ...
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bioRxiv - Cell Biology 2024Quote: ... ShOpa1 transduced cells were lysed and 35 μg of protein was electroblotted to PVDF membranes (EMD Millipore, Burlington, MA, USA). The membranes were blocked and incubated overnight at 4°C with antibodies against anti-BNIP3 (3769) ...
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bioRxiv - Genetics 2024Quote: ... Approximately 40 L4 stage mutant animals were then picked and placed on 35 mm OP50-seeded NGM plates containing 0.5 mM aldicarb (Sigma Aldrich, 33386 ...
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bioRxiv - Immunology 2024Quote: ... 8-Cyclopentyl-1,3-dipropylxanthine (DPCPX)(28, 33, 34) or A1 receptor agonist 2-Chloro-N6-cyclopentyladenosine (35) were purchased from Sigma Aldrich, dissolved in DMSO and filter sterilized by passing through a 0.22μm filter prior to use ...
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bioRxiv - Biochemistry 2021Quote: HEK293 cells (Sigma:85120602) were cultured using DMEM (Gibco™ ...
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bioRxiv - Biophysics 2023Quote: HEK293 cells (Sigma Aldrich) and were cultured in DMEM (Gibco ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... in which the plate was coated with anti–human Fc (Sigma, Catalog # FAB3700259), and after overnight incubation at 4°C ...
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bioRxiv - Bioengineering 2021Quote: ... The binding of VHH-Fcs/ACE2-Fc to S1 was detected using 100 µL 1 µg/mL HRP-conjugated goat anti-human IgG (Sigma, Cat#A0170). Finally ...
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bioRxiv - Bioengineering 2021Quote: ... plates were washed 10 times with PBST and the ACE2-Fc binding was detected using 1 µg/mL goat anti-human IgG (Fc specific) HRP conjugate antibody (Sigma, Cat#A0170) in 100 µL PBSCT ...
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bioRxiv - Neuroscience 2021Quote: ... a small hole was made on the flat end of the egg and either VPA (Sodium Valproate, Sigma Aldrich, 35 μmoles) or vehicle (double distilled injectable water ...
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bioRxiv - Cancer Biology 2019Quote: ... The resulting supernatant was collected and slowly dripped over 9 ml of a 35-30-25% sucrose gradient (Sigma, Milan, Italy) and centrifuged at 10,000 rpm for 2 h in a 15 ml Polyclear Centrifuge tubes (Seton USA) ...
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bioRxiv - Developmental Biology 2021Quote: Dissected wild-type and Smad4Δ/Δ forelimb buds from E10.0 embryos (30 somites) and 10.5 embryos (35 somites) were collected in RNAlater® (Sigma R0901), incubated overnight at 4°C and then stored at -80°C ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and washed in 0.05% v Brij-35 in PBS (pH 7.4) and immunostained using antibodies to MUC5AC (Millipore Inc., Burlington, MA), SCGB1A1 or secretoglobulin 1A1 (Santa Cruz Biotechnology ...
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Long-term cellular cargo tracking reveals intricate trafficking through active cytoskeletal networksbioRxiv - Biophysics 2022Quote: ... were cultured in a 35-mm confocal dish (SPL, Korea) pre-coated with 0.1 mg/ml poly-D-lysine (P7886, Sigma-Aldrich, USA) for good cell attachment ...
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bioRxiv - Molecular Biology 2022Quote: MHC Class I peptides previously identified to bind HLA-A*03:01 (TIAPALVSK) and HLA-B*35:03 (YPTTTISYL) in HCC38BL cells were purchased as ≥95% pure synthetic peptides from Sigma-Aldrich. These peptides were solubilized in DMSO and used for cell treatments at 20 ug/ml concentration ...
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bioRxiv - Microbiology 2019Quote: Proteins derived from rRVs-infected cellular extract were separated on an SDS-PAGE for 2 hours at 35 mA and transferred to polyvinylidene difluoride membranes (Millipore; IPVH00010) for 1 hour and 30 minutes at 300 mA (24) ...
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bioRxiv - Molecular Biology 2019Quote: ... Dissociated neurons were immediately plated onto 35 mm dishes coated with 50 μg/mL poly-D-lysine (P6407, Sigma Aldrich, Germany), at a density of 5×105 live cells with 2 mL plating medium per dish ...
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bioRxiv - Cancer Biology 2020Quote: ... The resulting supernatant was collected and slowly dripped over 9 ml of a 35-30-25% sucrose gradient (Sigma, Milan, Italy) and centrifuged at 10,000 rpm for 2 h in a 15 ml Polyclear Centrifuge tubes (Seton USA) ...
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bioRxiv - Plant Biology 2020Quote: ... and placed in Petri dishes filled with 0.5% agarose containing 35 mM β-mercaptoethanol (v/v) and 1.5 mg/mL of 5-bromo-4-chloro-3-indolyl-β-D-galactoside (Sigma). For X-gal staining ...
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bioRxiv - Cancer Biology 2020Quote: ... using a 20 min linear gradient from 5% B to 35% B (A: 20 mM ammonium formate (cat No. 70221, Sigma, USA) in water ...
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bioRxiv - Evolutionary Biology 2021Quote: ... was polymerized for 10-20 minutes at 37 °C and 80 °C using an adapted protocol for eukaryotic tubulin (35) by adding temperature equilibrated K-PIPES buffer and 2 mM GTP (Sigma-Aldrich). 2.5 μL of the polymerized sample was applied to the glow- discharged grids ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Thawed OdinTubulin protein was diluted to final concentration of 40-60 μM in pre-warmed polymerization K-PIPES buffer as previously described for eukaryotic tubulin (35) and polymerized for 10-20 min using 2 mM GTP (Sigma-Aldrich). 2.5 μl of the mixture was applied onto glow discharged grid STEM100Cu elastic carbon grids (Ohkenshoji co. ...
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bioRxiv - Neuroscience 2021Quote: ... Primary hippocampal neurons were plated at density of 120 cells/mm2 on 3.5-cm diameter Petri dishes (Falcon® 35 mm, 353001) treated for 24 h with poly-L-lysine (0.1 mg/ml; Sigma-Aldrich) in borate buffer (0.1 M) ...
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bioRxiv - Pathology 2021Quote: ... the Melgisorb was placed into a 1.5 mL microcentrifuge tube containing 35 μL of alginate lyase (Flavobacterium, Sigma-Aldrich A1603-100MG) in High Performance Liquid Chromatography (HPLC ...
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bioRxiv - Microbiology 2022Quote: 1 μg of un-labelled purified CVA9 was mixed with 1 - 3 μL of 35S labelled CVA9 (showing approximately 1000 CPM) and faf-BSA (Sigma-Aldrich) or buffer giving final concentrations as indicated in Table 1 was added to obtain final volume of 100 μL ...
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bioRxiv - Neuroscience 2023Quote: ... The following commercial primary antibodies were used: rabbit anti-Olig2 antibody and mouse anti-panNav mAb (clone K58/35) purchased from Sigma-Aldrich Chimie ...
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bioRxiv - Neuroscience 2022Quote: 50,000 NPCs were plated onto a 35 mm dish coated with 0.1 mg/mL PDL and 5ug/mL Fibronectin (Sigma Aldrich F1141) containing 1 mL of 30% Expansion media without or with drugs/growth factors ...
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bioRxiv - Bioengineering 2022Quote: ... Hydroxyproline content as a measure for collagen was quantified in scaffolds using a chloramine-T assay [35] with trans-4-hydroxyproline (H54409, Sigma-Aldrich) as reference ...
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bioRxiv - Cell Biology 2023Quote: ... which was previously shown to promote high phagocytic activity and MerTK expression.35 Fetal hMGL were cultured in DMEM (Sigma-Aldrich) supplemented with 1% GlutaMAXTM ...
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bioRxiv - Microbiology 2023Quote: ... Eggs were then maintained in 50 mL Falcon tubes (100 eggs/tube) overnight in 35 mL of sterile mineral water supplemented with 0.4 µg/mL methylene blue solution (Sigma Aldrich 50484). At 1 dpf ...
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bioRxiv - Cell Biology 2022Quote: ... promotes esophageal eosinophilic infiltrates and food impactions (28, 35) and oral administration of the carcinogen 4-nitroquinoline 1-oxide (4NQO; N8141, Sigma-Aldrich) induces esophageal tumors that recapitulate histological and molecular features of human ESCC (36 ...
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bioRxiv - Biophysics 2023Quote: ... The glass nanopore was then immersed in a similar buffer with the addition of 50% (w/v) Polyethylene Glycol (PEG) 35 kDa (ultrapure grade, Sigma Aldrich). The notation of the 50% PEG in the text refers to the utilization of 50% (w/v ...
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bioRxiv - Developmental Biology 2023Quote: ... bolting ap1 cal 35S::AP1-GR and drmy1 ap1 cal 35S::AP1-GR plants were induced daily with an aqueous solution containing 10 µM dexamethasone (Sigma-Aldrich), 0.01% (v/v ...
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bioRxiv - Synthetic Biology 2024Quote: ... The nanopipette was then immersed in a 100 mM KCl bath with the addition of 50% (w/v) polyethylene glycol (PEG) 35 kDa (ultrapure grade, Sigma Aldrich). A working electrode consisting of Ag/AgCl wire (0.25 mm diameter ...
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bioRxiv - Molecular Biology 2023Quote: ... a total of 0.4–0.5 μg of plasmid and dsRNAs were transfected into BmN4 cells (4–6 × 104 cells per glass bottom 35 mm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche) and cells were fixed 5–6 days later ...
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bioRxiv - Molecular Biology 2023Quote: ... Cleared lysates (13,000 rpm, 10 min, 4°C) were incubated with 35 µl anti-FLAG antibody (Sigma-Aldrich, #F1804-1MG, RRID:AB_262044) for 30 min at 4°C with rotation ...