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Citations for Millipore Sigma :
351 - 400 of 10000+ citations for High mobility group protein B1 HMGB1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: ... MCF7 in DMEM-Hi Glucose medium (Sigma-Aldrich) HCC1937 and ZR-75-1 in RPMI 1640 media (Gibco) ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% HI-FBS (Sigma-Aldrich, #12306C), 1% GlutaMAX (200 mM ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% HI-FBS (Sigma-Aldrich, #12306C), 1% GlutaMAX (200 mM ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% HI-FBS (Sigma-Aldrich, #12306C), 2% GlutaMAX (200 mM ...
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bioRxiv - Biochemistry 2024Quote: ... An anti-His-primary antibody (Sigma, 1:2,000) was incubated with the protein-treated lipid strips to detect Jps1 bound via its C-terminal His-tag ...
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bioRxiv - Cell Biology 2024Quote: ... +10% HI FBS(Sigma Aldrich, cat#F4135-500ML) + penicillin/streptomycin (Sigma ...
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bioRxiv - Neuroscience 2022Quote: All animals were randomly divided into four groups (15 rats in each group) with different anesthetic protocols as follows: 1- Anesthetized with an intraperitoneal injection of chloral hydrate (#C8383, Sigma-Aldrich, 320 mg/kg; CH group); 2- Anesthetized with isoflurane inhalation (Terrell ...
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bioRxiv - Genetics 2019Quote: ... The control group fed a basal diet and betaine group fed a diet supplemented with betaine (98% purity, B2629, Sigma Chemical Co., St. Louis, MO, USA) at the level of 10 g/kg according to previous publications (38 ...
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bioRxiv - Neuroscience 2021Quote: HEK293 cells were transfected at 80–90% confluency using PEI transfection reagent (Sigma) with 2 mg/ml PEI per mg of DNA ...
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bioRxiv - Cell Biology 2019Quote: HEK293 cells were transfected with plasmids using polyethylenimine according to manufacturer’s instructions (Sigma). Neurons were transfected by the Ca2+-phosphate method (53) ...
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bioRxiv - Molecular Biology 2021Quote: HEK293 cells were seeded on glass coverslips coated with poly-L-ornithine (Sigma) in clear 12-well plates (3 × 105 cells/well ...
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bioRxiv - Cell Biology 2022Quote: HEK293 Flp-In T-REx stable lines were cultured in DMEM (Sigma, D5671) supplemented with 10% FBS ...
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bioRxiv - Molecular Biology 2022Quote: ... HEK293 cells were transfected with lentiviruses particles containing Pin1 shRNA (Sigma-Aldrich, TRCN0000010577) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... HEK293 cells were transfected using Polyethylenimine (PEI,Sigma Aldrich; St. Louis, Missouri, USA). PEI and vector media were combined in a ratio of 5µL PEI and 0.5µg vector in 250µL of serum-free DMEM and incubated for 10 min at room temperature ...
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bioRxiv - Microbiology 2020Quote: HEK293 or 293T cells were cultured in Dulbecco’s modified Eagle’s medium (Sigma-Aldrich) supplemented with 10% fetal calf serum ...
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bioRxiv - Microbiology 2022Quote: ... and HEK293 cells and were grown in Dulbecco’s Modified Eagle Medium (DMEM) (Sigma) supplemented with 10% v/v foetal calf serum (FCS ...
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bioRxiv - Microbiology 2023Quote: HEK293 T cells were maintained in Dulbecco’s modified Eagle medium (DMEM; Sigma, D6429) supplemented with 10% heat-inactivated FBS (Atlanta Biologicals ...
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bioRxiv - Developmental Biology 2024Quote: HEK293 cells were seeded onto glass coverslips coated with Poly-L-Lysine (Sigma) and left to adhere overnight prior to transfection with plasmid DNA ...
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bioRxiv - Molecular Biology 2020Quote: ... mouse-anti-flavivirus group antigen (Millipore: MAB10216; RRID: AB_827205; 1:500), pan-flavivirus hyperimmune mouse acites fluid (HMAF ...
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bioRxiv - Bioengineering 2021Quote: ... Aldehyde groups were blocked with 50 mM ammonium chloride (Sigma, A9434) in PBS for 20 min ...
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bioRxiv - Microbiology 2020Quote: ... The treated group were given 50 mg/kg disulfiram (Sigma Aldrich) and 1 mg/kg zinc gluconate (Alfa Aesar ...
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bioRxiv - Microbiology 2019Quote: ... Free SH-groups were blocked by adding methyl methanethiosulfonate (MMTS, Sigma, 208795 ...
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bioRxiv - Genetics 2021Quote: ... Unreacted NHS groups were subsequently blocked using 1M Tris pH9 (Sigma) with 2 hours room temp incubation on a roller mixer ...
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bioRxiv - Immunology 2022Quote: ... another group was cultured in 1% Triton X-100 (Sigma-Aldrich). Percentage of the specific lysis was calculated according to the following formula ...
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bioRxiv - Cancer Biology 2020Quote: ... The negative control group was incubated with normal mouse IgG (Millipore). Samples were incubated with proteinase K buffer to isolate immunoprecipitated RNA ...
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bioRxiv - Cell Biology 2020Quote: ... group 2 with 100 μg LPS + 500 ng AdipoRon (Sigma-Aldrich) and experimental group 3 underwent vehicle (PBS ...
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bioRxiv - Cell Biology 2022Quote: ... and 3 groups treated with 40 μM CCCP (C2759, Sigma-Aldrich). The untreated groups ...
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bioRxiv - Microbiology 2022Quote: ... and free sulfhydryl groups carbamidomethylated using 5.5 mM chloroacetamide (Sigma-Aldrich). Proteins were digested with 0.5 µg sequencing grade endopeptidase LysC (Wako ...
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bioRxiv - Neuroscience 2023Quote: ... randomly assigned to either control or ouabain group (2 mM, Sigma, O3125 ...
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bioRxiv - Neuroscience 2023Quote: ... randomly assigned to either control or bumetanide group (20 μM, Sigma, B3023 ...
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bioRxiv - Immunology 2023Quote: ... Amine groups were activated by 0.5 M CDI (#115533; Sigma-Aldrich) in DMSO and the beads were incubated on a bench-top rotor for 1h at RT ...
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bioRxiv - Biochemistry 2023Quote: All chemicals were acquired from Sigma-Aldrich (Merck Group; Massachusetts, US) and all other materials from Thermo Fisher Scientific (Massachusetts ...
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bioRxiv - Neuroscience 2024Quote: ... Treatment groups received either 6.5 mg/kg of benserazide (Sigma, B7283) combined with 25 mg/kg of l-DOPA (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... Primary antibodies used were acetylated α−tubulin (1:500, clone 6-11-B1, Sigma-Aldrich, St. Louis, MO), rabbit polyclonal DNALI1 (1:100 ...
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bioRxiv - Microbiology 2021Quote: ... and extensively dialysed against HEPES-buffered saline and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag mAb, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (Bethyl).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... thereafter referred to as Mpp75Aa1.1-His) or without the C-terminal His tag (thereafter referred as Mpp75Aa1.1) was cloned into a pET24a vector (Novagen, Madison, WI) and expressed in Rosetta 2 (DE3 ...
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bioRxiv - Cell Biology 2022Quote: ... and extensively dialysed against HBS and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag monoclonal antibody, 70796, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (A3562 ...
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bioRxiv - Cell Biology 2020Quote: Caco-2 (human epithelial colorectal adenocarcinoma cell line) cells were purchased from ATCC and cultured in DMEM high glucose media (Sigma). The cells were trypsinized using 0.25% trypsin-EDTA and resuspended in the fresh media ...
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bioRxiv - Microbiology 2020Quote: All laboratory-adapted strains were maintained in human foreskin fibroblasts (HFF) cells in high glucose DMEM with sodium bicarbonate (Sigma) supplemented with 5% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2021Quote: ... Non-adherent cells were washed and the remaining monocytes were maintained in DMEM high glucose with 5% human serum (HS; Millipore) and P/S ...
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bioRxiv - Systems Biology 2020Quote: Human embyronic kidney cells (HEK-293 cells) were grown as adherent cultures in DMEM with high glucose (Sigma-Aldrich D5796), supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2023Quote: ... and HOS-ACE2/TMPRSS2 cells (HOS cells stably expressing human ACE2 and TMPRSS2)31,32 were maintained in DMEM (high glucose) (Sigma-Aldrich, Cat# 6429-500M ...
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bioRxiv - Plant Biology 2019Quote: ... and axr3-3 were expressed as 6X His-tag (N-terminal) fusion proteins in Escherichia coli strain Rosetta™ DE3 competent cells (Supplementary Table 4; Novagen, product code: 70954). These proteins were expressed in minimal media with 13C D-glucose and 15N ammonium chloride ...
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bioRxiv - Molecular Biology 2019Quote: ... and zinc binding proteins were eluted according to the supplied protocol using HIS-Select wash and elution buffers purchased from Sigma-Aldrich (Saint Louis, MO).
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bioRxiv - Microbiology 2020Quote: ... membrane-extracted proteins were incubated for 1.5 hours at 4°C with purified human IgG (Sigma) that had been previously coupled to CNBr-activated Sepharose beads (GE Healthcare) ...
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bioRxiv - Immunology 2023Quote: ... Recombinant lyophilized proteins were resuspended using 0.1% (w/v) human serum albumin (HSA) (A9731, Sigma-Aldrich) in sterile water ...
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bioRxiv - Molecular Biology 2023Quote: Antibodies to human proteins used in this study included: Actin (1:20,000 dilution (MAB1501, Millipore, RRID:AB_2223041)) ...
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bioRxiv - Plant Biology 2020Quote: Proteins were resolved on SDS-PAGE followed by immunoblotting using α-HA high affinity antibody 3F10 (Sigma) and α-c-Myc Antibody (9E10) ...
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bioRxiv - Biophysics 2020Quote: ... The (His)8-tag was cleaved by thrombin (Sigma) using 20 U per 1 mg GltPh in the presence of 5 mM CaCl2 at room temperature overnight ...
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bioRxiv - Biophysics 2021Quote: ... 6×His-tagged recombinant MSP1E3D1 (Sigma-Aldrich, Merck, USA) and FLAG-tagged recombinant mCardinal (kindly provided by Jakub Czapiński ...