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Citations for Millipore Sigma :
251 - 300 of 10000+ citations for High mobility group protein B1 HMGB1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: His-select Cobalt affinity beads (Sigma) were equilibrated in binding buffer (40 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... anti-His (Sigma-Aldrich, H1029, RRID:AB_260015), anti-Strep (IBA GmbH ...
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bioRxiv - Developmental Biology 2024Quote: ... anti-HIS (Sigma H1029 1:2,500) or anti-MBP (New England Biolabs E8032 ...
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bioRxiv - Neuroscience 2023Quote: ... His•Tag® (Millipore, 70796-3), anti-APP C-Terminal Fragment (BioLegend ...
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bioRxiv - Biochemistry 2023Quote: ... or anti-His antibody (EMD Millipore) as described previously [39] ...
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bioRxiv - Biochemistry 2023Quote: ... or anti-His antibody (EMD Millipore) as described previously [39] ...
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bioRxiv - Cell Biology 2020Quote: ... Lamin B1 shRNA was expressed from pLKO.1 shRNA-LMNB1.71 puro (SHCLND-NM_005573, Sigma-Aldrich) containing the sequence ...
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bioRxiv - Biochemistry 2023Quote: ... the SEC-purified CerS6-Nb22 complex was incubated with 120 µM fumonisin B1 (Sigma-Aldrich) for 10 minutes on ice prior to protein concentration and subsequently for an additional 2h on ice prior to grid preparation ...
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bioRxiv - Neuroscience 2022Quote: ... group was fed 5 mg artemia and 30 mg egg yolks from chicken (59% fat, 32% proteins, 2% carbohydrates; Sigma-Aldrich, MO, USA) for one or two months (Landgraf et al. ...
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bioRxiv - Immunology 2023Quote: ... OVOL1 protein expression were determined by rabbit anti-human OVOL1 antibody (Sigma Aldrich).
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bioRxiv - Microbiology 2023Quote: ... PhoQ-His and Flag-MgrB were detected with the anti-His and anti-Flag primary antibody (Sigma), respectively ...
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bioRxiv - Neuroscience 2021Quote: ... A 100x high purity N2 solution was made with 10 mg/ml human holo-transferrin (Sigma T4132), 0.5mg/ml human recombinant insulin solution (Sigma I9278) ...
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bioRxiv - Molecular Biology 2020Quote: Human HepG2 cell line was grown in Dulbecco’s Modified Eagle’s medium – high glucose (Sigma-Aldrich®, D6429) supplemented with 10% fetal bovine serum (PAN Biotech ...
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LIR-dependent LMX1A/LMX1B autophagy crosstalk shapes human midbrain dopaminergic neuronal resiliencebioRxiv - Cell Biology 2019Quote: HEK293T human embryonic kidney cells were maintained in high-glucose DMEM medium supplemented with 10% FBS (Sigma) at 37°C in 5% CO2 ...
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bioRxiv - Neuroscience 2021Quote: ... Both groups were treated with 10 mM metronidazole (Sigma) in the absence of light for 24-48 hrs ...
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bioRxiv - Bioengineering 2021Quote: ... Z-group removing agents lauroyl peroxide (LPO; Sigma, 97%) and hydrogen peroxide (H2O2 ...
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bioRxiv - Neuroscience 2022Quote: ... A control group received vehicle solution (0.9% NaCl; Sigma). Mice treated with MPTP ...
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bioRxiv - Cancer Biology 2024Quote: ... Treated group contained Aphidicolin (1ug/ml; # A4487 Sigma-Aldrich) and CB103 (100μM ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-CA125 epitope group A (Sigma, OC125, 1:200), anti-CA125 epitope group A (Fitzgerald ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The control group was set with 0.1% DMSO (Sigma), and the blank control was set with water ...
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bioRxiv - Molecular Biology 2019Quote: The recombinant IFNε protein was purified using a single-step High-Select High Flow (HF) nickel affinity chromatographic gel (Sigma-Aldrich, Cat. # H0537). The nickel affinity column (1.0 cm × 1.0 cm ...
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bioRxiv - Microbiology 2020Quote: ... Purified proteins were mixed as indicated in the figure and rotated with either HIS-Select HF Nickel Affinity Gel (Sigma-Aldrich) or Glutatione Sepharose 4B (GE healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... Unbound protein was washed off in PBST (3x 5 min) and bound protein was detected using HRP-conjugated anti-His antibody (1:2000 in 5% milk PBST; Novagen #71841). After 1 hour incubation at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... The unbound portion of this capture step was kept as HIS-free eGFP protein and conjugates and concentrated once on a 10K Amicon column (Millipore Sigma) by spinning for 6-8 minutes at 6000x g ...
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bioRxiv - Biochemistry 2024Quote: ... of GST and His tagged proteins were subjected to buffer exchange using Amicon Ultra 0.5 ml 10 kDa cutoff columns (Millipore Sigma, UFC501024) with five sequential rounds of concentration performed by centrifugation at 14000 g and 4 °C for approximately 10 minutes and dilution with “EB base” (5x ...
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bioRxiv - Neuroscience 2020Quote: HEK293 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Sigma) supplemented with 10% (v/v ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... HEK293 cells were maintained in Dulbecco’s Modified Eagle’s Medium (Sigma Aldrich) supplemented with 10 % fetal calf serum at 37°C/5% CO2 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... HEK293 cells were maintained in Dulbecco’s Modified Eagle’s Medium (Sigma Aldrich) supplemented with 10% fetal calf serum at 37°C/5% CO2 ...
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bioRxiv - Cancer Biology 2020Quote: ... HEK293 suspension cells were transfected with constructs using polyethylenimine (PEI, Sigma). HEK293 suspension cells were cultured at 37°C and 8% CO2 and 120 rpm ...
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bioRxiv - Molecular Biology 2022Quote: ... HEK293 and HeLa cells were cultured in DMEM media (Sigma, RNBK7590) supplemented with 10% free fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: HEK293 (CRL-1573-ATCC) were grown in DMEM (Sigma-Aldrich, USA) supplemented with 10 % FBS (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: HEK293 cells were seeded on poly-L-lysine (Sigma, Cat. #P8920) coated coverslips in 12-well plates ...
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bioRxiv - Biochemistry 2023Quote: HEK293 cells were maintained in DMEM F-12 Ham medium (Sigma) supplemented with 10% fetal bovine serum (GIBCO ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 and HeLa cells were transfected with Polyethyleneimine (PEI, Sigma Aldrich) as previously described (Braun et al. ...
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bioRxiv - Bioengineering 2020Quote: The effect of HMGB1 on inducing the migration of rat BMSCs were examined using a Trans-well plate (Millipo Sigma, St Louise, USA). The rat BMSCs in DMEM at passage 1 were seeded into the upper layer of insert well of the trans-well plate ...
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bioRxiv - Bioengineering 2020Quote: ... The lower layer of the trans-well plate was filled with 100 ng/ml of HMGB1-containing DMEM with various concentrations of FSP-ZM1 or AMD3100 (Sigma, St Louise, USA). After 24 hours ...
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bioRxiv - Bioengineering 2020Quote: ... finally various concentrations of HMGB1 (1-100 ng/ml) and a HMGB1 (1000 ng)-containing AS scaffold with 10 μM of 5-bromo-2’-deoxyuridine (BrdU; Sigma, St Louise, USA) were added into 1 ml of serum-free medium and cultured for additional 24 hours ...
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bioRxiv - Genetics 2019Quote: ... anti-acetylated tubulin antibody (mouse, clone 6-11-B1, Sigma-Aldrich, cat. no. T6793; 1:1,000), and anti-GT335 (mouse ...
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bioRxiv - Molecular Biology 2021Quote: ... SC-His+3AT is minimal medium of SC-His supplemented with 3-aminotriazole (3-AT, 0.5mM, Sigma-Aldrich).
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bioRxiv - Microbiology 2019Quote: ... Removal of His-tag was verified by western blot with monoclonal α-His –HRP (1:10000) antibody (Sigma).
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bioRxiv - Plant Biology 2020Quote: ... coli using a Nickel-His column (Sigma). A 5 mL culture was incubated at 37°C for 16 hours ...
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bioRxiv - Cell Biology 2020Quote: ... anti-His tag (HIS.H8, 1:1,000, Millipore). The HRP-conjugated anti-mouse (111–035-144 ...
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bioRxiv - Plant Biology 2020Quote: ... -His with or without 3AT (Sigma-Aldrich). Successful interactions were further screened on –Leu ...
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bioRxiv - Microbiology 2020Quote: ... The anti-his antibody (monoclonal #8722 Sigma) was used at 1/2000 concentration for immunoblot.
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bioRxiv - Biochemistry 2022Quote: ... and anti-His (1:3000) antibody (Sigma). HRP conjugated mouse IgG was used at 1:5000 dilution (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... and anti-His HRP conjugated (Sigma-Aldrich) antibodies ...
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bioRxiv - Microbiology 2021Quote: ... HIS-Select® Nickel Affinity resin (Sigma) and Streptactin column material (IBA GmbH ...
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bioRxiv - Immunology 2020Quote: ... cOmplete His-Tag Purification Resin (Sigma Millipore) was applied for affinity purification ...
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bioRxiv - Immunology 2020Quote: ... cOmplete His-Tag Purification Resin (Sigma Millipore) was applied for affinity purification ...
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bioRxiv - Plant Biology 2020Quote: ... and purified on His•Bind resin (Novagen) in the presence of 8 M urea according to manufacturer’s instructions ...