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Citations for Millipore Sigma :
3851 - 3900 of 10000+ citations for NKX2 4 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Each recombinant protein was eluted twice in 250 μL EB containing 1 mM DTT and 250 μg/mL 3×FLAG peptide (Sigma-Aldrich). Eluates were pooled and concentrated using a Vivaspin 500 (GE Healthcare).
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bioRxiv - Biochemistry 2022Quote: ... the digested tryptic peptides from the pull-down samples and from the control group were desalted using C18 ziptips (EMD Millipore). Samples were further reconstituted in liquid chromatography-mass spectrometry (LC-MS ...
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bioRxiv - Bioengineering 2019Quote: ... a machined 48-vial rack holding 5 mL fritted peptide synthesis vessels (Torviq SF-0500) with 500 µL dimethylformamide (DMF; Sigma-Aldrich) in each was placed on the deck’s plate slot ...
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bioRxiv - Genetics 2020Quote: ... Protein-DNA complexes were eluted from the beads by adding 200 μL of ice-cold M2 buffer without detergent containing 3xFLAG peptides (Sigma-Aldrich) (final concentration of 150 ng/μL ...
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bioRxiv - Cancer Biology 2021Quote: ... and Ago1-4 were pulled down using 500 μg of Flag-GST-T6B peptide (50) and with 80 μl anti-Flag M2 Magnetic beads (Sigma #M8823) for 3 hrs on a rotor at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Beads were washed 5 times with RNase-free lysis buffer followed by elution with 50 µl RNase-free lysis buffer supplemented with 100 µg/ml 3xFLAG peptide (Sigma #F4799) for 30 min at 4°C on a rotating wheel ...
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bioRxiv - Neuroscience 2019Quote: ... Analysis by Western blot shows a band at 19 kDA from rat olfactory bulb which is blocked by inhibition with specific OMP immunizing peptide (Sigma datasheet).
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bioRxiv - Cell Biology 2020Quote: ... The EGFP-FLAG-tagged proteins were eluted from the beads with 0.1 mg/mL 3x FLAG peptide (Sigma-Aldrich, Cat. #F4799) in TBS at 4°C for more than 1 hr ...
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bioRxiv - Biochemistry 2021Quote: ... Rabbit anti-SNAP-25 polyclonal antibody raised against a synthetic peptide corresponding to the N-terminus of human SNAP-25 (Sigma S9684), was used as a positive control to detect both cleaved and intact SNAP-25 ...
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bioRxiv - Biochemistry 2021Quote: ... The proteins were then eluted from the beads with 100 μL of lysis buffer containing 100 μg/mL 3 × FLAG peptide (F4799, Sigma Aldrich), mixed with sample buffer and incubated on ice for 30 min ...
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bioRxiv - Biochemistry 2021Quote: ... PAR chains from a purified tetra-ADP-ribosylated H2B (1-16) peptide were subjected to treatment with Alkaline Phosphatase (Sigma-Aldrich) and Phosphodiesterase I (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... FITC-labelled peptide samples (Li, i+4L, Li, i+4D or the RCM control) were dissolved in 100% rat serum (Sigma Aldrich) at an initial concentration of 4 μM ...
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bioRxiv - Microbiology 2019Quote: Peptidoglycan-derived stem peptide fragments including ‘A-E-K-A-A’ pentapeptide (L-Ala-D-Glu-L-Lys-D-Ala-D-Ala (Sigma-Aldrich), ‘K-A-A’ tripeptide (acetyl-L-Lys-D-Ala-D-Ala ...
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bioRxiv - Microbiology 2020Quote: ... The resin was washed 5 times and transferred to a spin column with 40 μl of 3 X Flag peptide (Sigma, #F4799) for 1 h at 4°C on a rotator ...
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bioRxiv - Immunology 2020Quote: PBMC or Expanded T cell lines were stimulated for 5h at 37°C with or without SARS-CoV-1/2 peptide pools (2 μg/ml) in the presence of 10 μg/ml brefeldin A (Sigma-Aldrich). Cells were stained with the yellow LIVE/DEAD fixable dead cell stain kit (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... the specificity of the CRF antibody in these tissues was demonstrated by preadsorption with 10X and 100X CRF peptide (Sigma-Aldrich), both of which eliminated CRF immunoreactivity [15].
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bioRxiv - Molecular Biology 2020Quote: ... The recombinant xLIG1 was eluted twice in 250 μl EB containing 1 mM DTT and 250 μg/ml 3×FLAG peptide (Sigma-Aldrich).
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bioRxiv - Immunology 2020Quote: Expanded T cell lines were stimulated for 5h at 37°C with or without SARS-CoV-2 peptide pools (2 μg/ml) in the presence of 10 μg/ml brefeldin A (Sigma-Aldrich). Cells were stained with the yellow LIVE/DEAD fixable dead cell stain kit (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... The emulsion was prepared by mixing MOG35-55 peptide (United Biosystems, Cat# U104628) and complete Freund’s adjuvant (Sigma-Aldrich, Cat# F5881) with additional Mtb (BD Difco ...
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bioRxiv - Biochemistry 2022Quote: ... The recombinant xDnmt1 was eluted twice in 250 µL EB containing 1 mM DTT and 250 µg/mL 3×FLAG peptide (Sigma-Aldrich). Eluates were pooled and concentrated using a Vivaspin 500 (GE Healthcare Biosciences).
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bioRxiv - Immunology 2022Quote: ... CD8 T cells were stimulated with 1 µg/ml of gp33 peptide in the presence of 10 µg/ml Brefeldin A (Sigma-Aldrich) at 37°C for 6 h ...
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bioRxiv - Immunology 2022Quote: ... The proteins captured on the anti-FLAG resin were eluted by incubation with 46 μl of TBS containing 150 ng/µL of 3X FLAG peptide (Sigma, #F4799). For mass spectrometry (MS ...
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bioRxiv - Neuroscience 2022Quote: ... Peptides were washed two times with 200 µl 1% TFA 99% ethyl acetate (ThermoFisher Scientific; Cat#: 28904; Millipore Sigma; Cat#: 270989) followed 200 µl 0.2% TFA/5%ACN (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... The desalted peptides in the cartridge were eluted using a buffer containing 0.5% acetic acid and 80% acetonitrile (Sigma-Aldrich-Aldrich). The samples were subsequently dried using a speedvac for mass spectrometry analysis.
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bioRxiv - Biochemistry 2023Quote: ... Beads were then washed three times with lysis buffer and eluted in TB buffer supplemented with 0.5mg/mL 3xFlag-peptide (SIGMA F4799-4MG). Eluted proteins were precipitated by adding 20% TCA followed by overnight incubation on ice ...
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bioRxiv - Biochemistry 2024Quote: ... numbering includes the signal peptide) with C-terminal Flag and 6x-Histidine tags and subsequently cloned into a p32A vector (Millipore Sigma). The TrxA-3C-ligand-Flag-6xHis construct was transformed into Origami E ...
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bioRxiv - Cancer Biology 2023Quote: ... 50 μg of biotinylated peptides were bound to 25-50 μl of a 50:50 slurry of streptavidin agarose (Sigma-Aldrich), mixed with recombinant proteins or cell lysates ...
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The tetrapeptide sequence of IL-1β regulates its recruitment and activation by inflammatory caspasesbioRxiv - Immunology 2023Quote: ... Proteins were eluted by rotating the resin at room temperature for 1 hour in 100 μL of PBS containing 150 ng/ μL 3×-FLAG peptide (Sigma Aldrich). 100 μL of 2x sample loading buffer was added to the eluate and samples were boiled at 95 °C for 10 minutes ...
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bioRxiv - Neuroscience 2022Quote: Seven-week isogenic control- and healthy control hiPSC derived neurons were treated with 2.5 μM Aβ peptide 1-42 (Sigma, PP69-0.25MG) for 24 hours.
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bioRxiv - Immunology 2023Quote: ... 18 h after cell transfer recipient mice were immunized into the right hind paw with 50 μl of a 1:1 emulsion of 1 mg/ml MOG35-55 peptide (M4939, Sigma Aldrich) in distilled water and complete Freund’s adjuvant (incomplete Freund’s adjuvant (Sigma ...
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bioRxiv - Immunology 2023Quote: ... injected into the left and right flank with 100 μl of an emulsion of MOG35-55 peptide (500 μg/ml) (M4939, Sigma Aldrich) in distilled water and complete Freund’s adjuvant (made from incomplete Freund’s adjuvant (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... and the bound proteins were eluted with buffer H containing 0.1 M NaCl and 100 μg/mL of FLAG peptide (Sigma Aldrich, #F3290). Peak fractions were pooled and loaded onto a MonoQ 5/50GL column (Cytiva ...
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bioRxiv - Biochemistry 2023Quote: ... the affinity columns were eluted with 500ul of 0.5ug/ul FLAG peptide prepared according to the manufacturer instruction (Sigma-Aldrich #F3290).
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bioRxiv - Genetics 2023Quote: Liver protein isolation and western blots on liver extracts and plasma samples were performed as previously described.17 Primary antibodies to the 2A peptide (1:5000, rabbit, ABS31, Sigma-Aldrich), GFP (1:3000 ...
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bioRxiv - Genetics 2023Quote: ... as previously described.17 The following primary antibodies were used for immunohistochemistry: rabbit anti 2A peptide (1:7500, ABS31, Sigma-Aldrich), rabbit anti Ki67 (1:60 ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoprecipitates were washed three times with RIPA buffer and Flag-tagged proteins were eluted by two consecutive elution steps with 400 µg/ml Flag-peptide (Sigma-Aldrich) for 30 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... cells were stimulated with 10nM SIINFEKL (OVA) peptide for 5h at 37°C in the presence of Brefeldin A (Sigma–Aldrich) for the last 2h before staining ...
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bioRxiv - Immunology 2023Quote: ... Each group (n = 6) was immunized intraperitoneally with peptides-KLH (10 μg/mouse) emulsified in Incomplete Freund’s Adjuvant (Sigma, Cat.#F5506), and a control group (n = 6 ...
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bioRxiv - Biochemistry 2023Quote: ... HEK293T cells used for proteome-derived peptide library generation were tested every six months for mycoplasma contamination using the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich) according to the manufacturer’s instructions (Fig ...
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bioRxiv - Cell Biology 2023Quote: ... and SKR-2-containing protein complexes were eluted overnight in 100 µl of the lysis buffer containing 2 mg/mL of 3xFlag peptide (Sigma F4799). Eluted protein samples were digested with trypsin and analyzed by MudPIT in the Mass Spectrometry and Proteomics Facility at Johns Hopkins School of Medicine.
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Arabidopsis actin-binding protein WLIM2A links PAMP-triggered immunity and cytoskeletal organizationbioRxiv - Plant Biology 2023Quote: ... The desalted peptide solution was analyzed by LC-MS/MS using a C18 ZipTip® (Millipore, Burlington, MA, Cat. No. ZTC18S096).
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bioRxiv - Cancer Biology 2023Quote: ... Protein complexes were eluted by two rounds of thirty minute incubations on ice with 200 ng per μL 3X FLAG Peptide (SIGMA, F4799) in TBS.
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bioRxiv - Cancer Biology 2023Quote: ... Protein complexes were eluted by two rounds of thirty minute incubations on ice with 200 ng per μL 3X FLAG Peptide (SIGMA, F4799) in Native Elution Buffer.
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bioRxiv - Molecular Biology 2023Quote: ... Each recombinant protein was eluted twice in 250 µl of EB containing 1 mM DTT and 250 µg/ml 3×FLAG peptide (Sigma-Aldrich). Eluates were pooled and concentrated using a Vivaspin 500 (GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... before incubation in 60 µL 3xFLAG elution solution (146 ng/µL of 3xFLAG peptide [Sigma-Aldrich, F4799] in 0.97X equilibration buffer) at 25°C with shaking at 1,100 rpm for 1 h ...
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bioRxiv - Biophysics 2024Quote: ... NEDD4-2 HECT domain was subsequently isolated from the supernatant with anti-FLAG antibody (M2) affinity chromatography and eluted with 100 μg/mL FLAG peptide (Sigma Millipore) in 50 mM TrisHCl ...
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bioRxiv - Cancer Biology 2024Quote: ... Peptide clean-up was performed on mixed-phase columns (PreOmics®) according to the manufacturer’s protocol using triethylamine (TEA, Sigma-Aldrich) instead of NH4OH in the elution buffer due to subsequent TMT labelling ...
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bioRxiv - Biochemistry 2024Quote: ... and Cdc13 was eluted by incubating the resin with 2 mL of K buffer supplemented with 200 µg/mL of 3x FLAG peptide (Sigma-Aldrich). Eluates were pooled and concentrated using Amicon Ultra 0.5 mL 100 k MW cutoff centrifuge filters (Millipore) ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were treated with 50-70 µL blocking solution (5% normal donkey serum [NDS, ImmunoReagents, SP-072-V×10], 1% Bovine Serum Albumin (BSA, Sigma-Aldrich, A1595-50ML), 8.5 mL PBS ...
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bioRxiv - Cell Biology 2020Quote: ... cells were permeabilized for 1 min with 0.1% Triton X-100 in PBS and saturated with blocking buffer (1% BSA in PBS) before incubation for one hour with anti-α-Tubulin (T5168. SIGMA; 1:2.000) in PBS containing 1% BSA ...