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Citations for Millipore Sigma :
3801 - 3850 of 10000+ citations for NKX2 4 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... The supernatants were collected and incubated for 4 h at 4 °C on a rotating wheel with 40 μl of packed anti-FLAG M2 affinity gel (Sigma, #A2220), which had been washed three times with lysis buffer prior to use ...
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bioRxiv - Microbiology 2022Quote: ... were place on the plate and 10 μL of (1, 2, 4 and 9.8 M) H2O2 and (2, 4, 6 M) methyl viologen (Sigma-Aldrich, UK) was dispensed onto each separate disk ...
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bioRxiv - Evolutionary Biology 2022Quote: ... erythrogramma embryos were fixed for in-situ hybridization (ISH) for ∼16 hrs overnight at 4 °C in 4% paraformaldehyde (Sigma 158127) + 20 mM EPPS (Sigma E1894 ...
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bioRxiv - Neuroscience 2024Quote: ... The membranes were then blocked in 5% milk in Tris Buffered Saline with 1% Tween (TBS-T) overnight at 4° C before being probed overnight at 4° C with Rabbit anti Magi-2 (1:1000, Sigma M4221); Mouse anti-NR1 (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were fixed with 4% paraformaldehyde for 10 min and treated with 4’,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich) diluted to a 1/10,000 concentration from the stock solution ...
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bioRxiv - Cancer Biology 2023Quote: Organoids were seeded in 15-well Ibidi chambers after passaging and fixed 3-4 days later in 4% Paraformaldehyde (Sigma-Aldrich) for 60 minutes at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... centrifuged (500g at 4°C for 4 minutes) and resuspended in PBS containing 1 μg/mL DAPI (Sigma Aldrich, D9542-100MG). Analysis of flow cytometric data was preformed using the BD Fortessa X20 and FlowJo v10 software (Ashland) ...
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bioRxiv - Cell Biology 2023Quote: Cells were harvested and pelleted by centrifugation for 4 minutes at 800 x g and washed twice in vPBS before being resuspended in fixation solution consisting of 4% formaldehyde and 4% acrylamide and transferred to a 12 mm round coverslip (Sigma, P4707) coated in poly-L-lysine and placed in a 24 well plate ...
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bioRxiv - Molecular Biology 2023Quote: ... Newly synthesized RNA was then labelled by incubating the cells for 1h with 100 µM 4-thiouridine (4-sU, Sigma-Aldrich). RNA extraction was performed with peqGOLD TriFast Reagent (VWR ...
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bioRxiv - Immunology 2023Quote: ... The FLAG-tagged WT sCTLA-4 or Y139A sCTLA-4 in the culture supernatant was purified with the anti-FLAG M2 affinity gel (Sigma-Aldrich), washed with wash buffer (Phosphate buffered saline ...
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bioRxiv - Plant Biology 2023Quote: ... Supernatants were incubated for 3-4 h at 4 °C with 15-20 μL of ANTI-FLAG® M2 Affinity Gel (Sigma) and washed 3-4 times with Co-IP wash buffer containing 0.1% Triton-100 but without Phosphatase Inhibitor Cocktails and Protease Inhibitor Cocktail ...
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bioRxiv - Cell Biology 2023Quote: ... in combination with 150 μg/ml heparin (Gilvasan, 1000 IE/ml) or 40-μM 4-aminobenzoic acid hydrazide (4-ABAH) (Sigma-Aldrich) where applicable.
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bioRxiv - Cancer Biology 2022Quote: Primary tumor samples and lungs were fixed in 4% paraformaldehyde (PFA) at 4°C for 24 h and decalcified in 14% EDTA (Sigma-Aldrich) for up to 28 d at 4°C ...
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bioRxiv - Immunology 2023Quote: ... melanoma was induced by injecting B6.Cg-Tg(Tyr-cre/ERT2)13Bos BRAFtm1Mmcm Ptentm1Hwu/BosJ (BRAFV600E PTEN-/- Tyr::CreERT2, Strain #013590) mice subdermally with 4-hydroxytamoxifen (4-HT)(Millipore Sigma, H6278 ...
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bioRxiv - Bioengineering 2023Quote: ... or 5 µg/mL NETs for 4 hr prior to fixation with 4% paraformaldehyde (Electron Microscopy Services, Hatfield, PA) in phosphate-buffered saline (PBS, Sigma-Aldrich) for 15 min at room temperature and staining with 0.005% Crystal Violet in PBS (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... and fixed overnight at 4°C in phosphate buffer saline solution (PBS) containing 4% (vol/vol) paraformaldehyde (cat# P6148, Sigma-Aldrich). Layers of tissue containing the myenteric plexus were separated by microdissection ...
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bioRxiv - Molecular Biology 2024Quote: ... at 6-8 weeks of age via intraperitoneal injections (100 μL of 4 mg/mL solution per day for 4 consecutive days) dissolved in corn oil (Sigma, C8267). All mice were used in mixed genetic backgrounds ...
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bioRxiv - Bioengineering 2024Quote: ... the density of amines on the surface of the coverslips was quantified using UV absorbance of 4-nitrobenzaldehyde (4-NB, Sigma Aldrich) attached to amines on functionalized coverslips as we have reported elsewhere.[31] Calibration curves of absorbance of 4-NB were generated reading spectra from a UV-Vis Spectrophotometer (Varian Cary 50 Bio) ...
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bioRxiv - Neuroscience 2024Quote: ... In Aldh1l1-CreER;mGluR5fl/fl;GCaMP6s and Aldh1l1-CreER;mGluR3fl/fl;GCaMP6s mice (Z)-4-Hydroxytamoxifen (4-OHT; H7904; Sigma-Aldrich) was used ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were extracted and post-fixed for 1 day in 4% PFA at 4°C before transferring to 30% sucrose (Millipore Sigma) in PBS at 4°C ...
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bioRxiv - Immunology 2024Quote: ... The filtered supernatant was then concentrated using a centrifugal filter unit with a 3 kDA cutoff at 4°C (Amicon Ultra-4 Centrifugal filters, Millipore, USA). Concentrated supernatant was stored in 200 µL aliquots at −80°C until further analysis.
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bioRxiv - Cell Biology 2024Quote: ... The lysates were centrifuged at 15,000 × g for 15 min at 4 °C and were incubated for overnight at 4 °C with anti-FLAG M2 antibody (F1804, Sigma-Aldrich). Subsequently ...
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bioRxiv - Developmental Biology 2024Quote: ... On the 7th day 1 µM Senexin B (SenB, Senex Biotechnology, Columbia, SC) or 1 µM 4-hydroxytamoxifen (4-OHT, Sigma-Aldrich) was added to the cells ...
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bioRxiv - Biophysics 2021Quote: A 3 μL droplet of the 500 μM peptide solution was applied to glass slides and stained with a 10 mM Congo Red (Sigma©) solution in PBS (137 mM NaCl ...
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bioRxiv - Microbiology 2019Quote: ... The eluted tryptic peptides were dried by speed-vacuum centrifugation and then desalted through ZipTip C18 Pipette tips (Millipore/Sigma-Aldrich). Samples were reconstituted in 10 µL of 0.1% formic acid before their analysis by nLC–MS/MS ...
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bioRxiv - Microbiology 2019Quote: ... The eluted tryptic peptides were dried by speed-vacuum centrifugation and then desalted through ZipTip C18 Pipette tips (Millipore/Sigma-Aldrich). Samples were reconstituted in 10 µL of 0.1% formic acid before their analysis by nLC–MS/MS ...
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bioRxiv - Developmental Biology 2021Quote: ... The FLAG tagged proteins were then eluted in the FLAG wash buffer containing a final concentration of 500 ng/μl of FLAG peptide (Sigma, F4799).
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bioRxiv - Biochemistry 2020Quote: Peptides were desalted and concentrated with C18 ZipTips (10 μL pipette tip with a 0.6 μL resin bed; Millipore, MA, USA). Samples were dried and reconstituted in 25 or 50 μL 0.1% formic acid depending on the specific analysis ...
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bioRxiv - Immunology 2022Quote: PBMC or expanded T cell lines were stimulated for 5h at 37°C with or without SARS-CoV-2 peptides (2 μg/ml) in the presence of 10 μg/ml brefeldin A (Sigma-Aldrich). Cells were stained with the yellow LIVE/DEAD fixable dead cell stain kit (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... Two elution steps of the FLAG-tagged protein-DNA complexes were performed with 200 μL of FLAG buffer (TBS buffer plus 100 μg/ml of 3XFLAG peptide from Sigma-Aldrich) and the samples were incubated 30 min at RT on a rotating wheel (20 rpm) ...
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bioRxiv - Genetics 2019Quote: ... We used the anti-HA high affinity monoclonal antibody from rat to recognize the HA-peptide (clone 3F10, ref 11 867 423 001, Sigma-Aldrich).
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bioRxiv - Microbiology 2019Quote: ... Beads were washed 5 times in NP40 buffer and precipitated proteins were eluted by boiling with SDS sample loading buffer or elution with 3X FLAG peptide (Sigma-Aldrich). Whole cell lysates and immunoprecipitated samples were analyzed by western blot.
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bioRxiv - Biochemistry 2019Quote: ... and eluted by competitive elution in Flag Wash Buffer supplemented to 200 ug/mL Flag peptide and 250 ug/mL human insulin as a carrier (Sigma-Aldrich). The protein was then passed over a 2 mL amylose column (NEB ...
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bioRxiv - Immunology 2021Quote: ... were coated with 50 µL/well of 1 ng/µL solution of fragments 308-331 or 421-438 from HIV gp120 peptides or fragment 579-601 of HIV gp41 (Sigma-Aldrich) in carbonate-bicarbonate buffer (pH 9.6 ...
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bioRxiv - Cell Biology 2020Quote: ... and eluted with FLAG elution buffer [identical to FLAG wash buffer but also containing 3x FLAG peptide (100 µg/ml, Sigma Aldrich)] ...
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bioRxiv - Bioengineering 2020Quote: ... Bound proteins were eluted by competition with four 2 x CV of DPBS containing 100 µg/mL FLAG peptide (Sigma Aldrich). Purified proteins were formulated in DPBS by buffer exchange through desalting columns (GE Healthcare) ...
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bioRxiv - Biophysics 2021Quote: ... All experiments were prepared in PBS and contained either 0.2 μM of the respective DegP enzyme (monomer concentration) and 7.5 μM of p23 peptide or 10 μM of the enzyme with 50 μM β-Casein (Sigma-Aldrich) and 100 μM of the Abz-KASPVSLGYNO2D reporter peptide ...
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bioRxiv - Neuroscience 2021Quote: ... Arc-Myc-FLAG was eluted by incubating beads in 50 µL of RIPA buffer containing 25 µg of FLAG peptide (Sigma-Aldrich) for 2 h at 25°C with gentle agitation ...
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bioRxiv - Plant Biology 2021Quote: ... Samples were washed three times with the protein elution buffer (20 mM Tris-HCl, 150 mM NaCl, 10% glycerol) and eluted by adding 3× FLAG peptide (Sigma-Aldrich) in 60 µL of the protein elution buffer following the manufacturer’s instruction.
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bioRxiv - Microbiology 2021Quote: ... The beads were washed 5 times in RIPA buffer and the immunoprecipitated proteins eluted using 150 μg/mL 3x Flag peptide (Sigma-Aldrich) in elution buffer (50 mM Tris/HCl pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... subtilis was achieved by 5 min incubation with small cation specific channel-forming antimicrobial peptide Gramicidin ABC (10 µM; gABC; Sigma Aldrich), and membrane permeabilisation by 5 min incubation with pore-forming lantibiotic Nisin (10 μM ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated with increasing concentrations (0 to 120 μg/ml) of a reverse sequence control peptide (Ser-Asp-Gly-Arg-Gly/SDGRG, Sigma-Aldrich) and an RGD peptide (Gly-Arg-Gly-Asp-Ser/GRGDS ...
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bioRxiv - Molecular Biology 2022Quote: ... Each recombinant protein was eluted twice in 250 µl of EB containing 1 mM DTT and 250 µg/ml 3xFLAG peptide (Sigma-Aldrich). Eluates were pooled and concentrated using a Vivaspin 500 (GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: ... in-house identifier: AfR364-3P2) and hCAP- D3 pS1474 peptide (C-QQWNVRpSPARNK; in-house identifier: AfR358-3P2) were generated commercially (SIGMA Genosys). Secondary antibodies used in the current study were as follows ...
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bioRxiv - Microbiology 2021Quote: ... were mixed with 100 μl of individual peptide pools in 96-well plates and incubated at 37°C for 1 h after which brefeldin A (Sigma Aldrich) was added and incubation continued for 4-5 h ...
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bioRxiv - Immunology 2020Quote: ... C57BL/6J mice were injected subcutaneously in the flanks with 100 μg of MOG35-55 peptide (Polypeptide) emulsified in 100 μl of CFA (Sigma-Aldrich) supplemented with 50 μg of heat-killed Mycobacterium tuberculosis H37Ra (BD Biosciences) ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated with 500 μg Flag-GST-T6B peptide [46] and 80 μl of anti-Flag M2 Magnetic beads (Sigma, #M8823) for at least 3 hours rotating at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... and sDCs were stimulated for 18 h with 1µg/ml of SIINFEKL peptide (OVA 257-264, Proimmune) or with 1mg/ml of low endotoxin soluble ovalbumin protein (sOVA; Sigma-Aldrich) in combination with NaCl or 5 ng/ml of IFNα ...
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bioRxiv - Cell Biology 2022Quote: ... 2% glycerol) and then eluting by shaking with 15 μL of 100 μg/μL of 3X FLAG peptide (Sigma-Aldrich, F4799) in the original lysis buffer for 30 min at 4°C.
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bioRxiv - Molecular Biology 2022Quote: MHC Class I peptides previously identified to bind HLA-A*03:01 (TIAPALVSK) and HLA-B*35:03 (YPTTTISYL) in HCC38BL cells were purchased as ≥95% pure synthetic peptides from Sigma-Aldrich. These peptides were solubilized in DMSO and used for cell treatments at 20 ug/ml concentration ...