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Citations for Millipore Sigma :
3751 - 3800 of 10000+ citations for Cyclohexyl 2 3 4 5 trifluorophenyl ethyl ketone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... the slides were washed with TBS and incubated with 1 mg/ml 4’,6-diamidino-2-phenylindole (DAPI) (Sigma) for 5 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... Nuclei were labeled by incubation in PBS containing 4′,6-diamidino-2-phenylindole (DAPI) (0.1 μg/ml) (Sigma-Aldrich), and samples were mounted in ProLong Gold Antifade Mountant (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2023Quote: ... in absence or in presence of 50 µM pyridoxal-phosphate-6-azopheny-2’,4’-disulfonate (PPADS) (#P178, Sigma-Aldrich), 100 µM oxidized ATP (OxATP ...
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bioRxiv - Cell Biology 2023Quote: ... Medium samples from day 2 and day 4 had to be diluted 1:1 in PBS (P4417, Sigma-Aldrich) because of the higher levels of cell death in the first days after harvesting caused by the isolation procedure ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were incubated at 37 ℃ and 5% CO2 for 2 weeks and were stained with 500 µl of 4% formaldehyde and 0.005% crystal violet (C3886, Sigma) for 4 hours ...
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Beclin1-Deficient Adipocytes Promote Tumor Progression by YAP/TAZ-dependent Adipocyte TransformationbioRxiv - Biochemistry 2023Quote: ... the cells were mounted on glass slides using Fluoroshield with 4’,6-diamidino-2-phenylindole (DAPI) (F6057; Sigma-Aldrich). Conventional fluorescent imaging was performed using the Axio Oberver.Z1/7 (Carl Zeiss) ...
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bioRxiv - Immunology 2023Quote: ... Serum and supernatant from homogenized tissues were incubated overnight at 4°C with 2% Trichloroacetic acid solution (Sigma, #T0699) at a 1:1 dilution ...
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bioRxiv - Biochemistry 2022Quote: Phagosomal proteins from RAW264.7 cells were extracted in 1% sodium 3-[(2-methyl-2-undecyl-1,3-dioxolan-4-yl)methoxy]-1-propanesulfonate (commercially available as RapiGest, Waters) in 50 mM Tris (Sigma) pH 8.0 ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 mM Glucose) containing 1µg/mL of 4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI) (Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Immunology 2024Quote: ... cell nuclei were stained with 1 μg/ml 4′,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich, Cat. No. D9542) diluted in PBS ...
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bioRxiv - Developmental Biology 2023Quote: ... Sections were washed with PBS and incubated with secondary antibodies for 2 h at RT in 2.5% BSA and 0.1% triton-X in PBS followed by counterstaining with 4’,6-diamidino-2-fenylindool (DAPI) (1µg/µl, Sigma, D9564) for 15 minutes at RT ...
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bioRxiv - Genetics 2023Quote: ... The seminiferous tubules were incubated for 60 min at RT in fixation buffer (100 mM HEPES pH7.4, 4 mM CaCl2, 2.5% glutaraldehyde, 2% PFA, all from Sigma Aldrich) and then the buffer was exchanged with fresh fixation buffer and the samples left overnight fixed at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... 4°C) and resuspended in 1-1.5 ml chilled wash and resuspension buffer containing 2% Bovine Serum Albumin (Sigma) and 0.2 U/μl RNase Inhibitor (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... and the supernatant was incubated for 2 h at 4 °C with 150 μL streptavidin-agarose beads (Sigma-Aldrich) under gentle agitation ...
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bioRxiv - Microbiology 2022Quote: ... 20 adult female flies (2–4 days old) per vial were fed with 50ug/mL of RU486 (M8046, Sigma; stock solution is 10mg/mL in DMSO ...
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bioRxiv - Neuroscience 2024Quote: ... Mice were then perfused through the left ventricle with ice-cold 0.1 M phosphate buffer saline (PBS; pH 7.4, 4 °C) supplemented with phosphatase inhibitors (Phosphatase Inhibitor Cocktail 2; Sigma), protease inhibitors (Complete EDTA-free Tablets ...
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bioRxiv - Immunology 2024Quote: ... and the bronchoalveolar lavage (BAL) fluid was collected in 0.5+0.6×4 ml 2% BSA (Sigma, St. Louis, MO). The supernatant of the initial 0.5 ml was stored for cytokine detection ...
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bioRxiv - Microbiology 2024Quote: ... the cells were washed four times in PBS and incubated with 4’,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich) in 200 ng/mL PBS for 15 minutes at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were washed with PBS and mounted using FluoroshieldTM with DAPI (4’,6-diamidino-2-phenylindole) (Sigma, F6057-20ML) (Pinto et al. ...
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bioRxiv - Cancer Biology 2024Quote: ... for 15 min at 4 °C and processed for immunoprecipitation as described previously13,15 using 2 μg PROX1 or control IgG (Sigma) antibody per reaction (Supplementary Table 11) ...
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bioRxiv - Cell Biology 2024Quote: ... Deletion of dynamin proteins in dynamin tKO MEFs were achieved by supplement of 2 μM 4-hydroxytamoxifen (Sigma; H6278) in the GM for 72 days ...
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bioRxiv - Genetics 2024Quote: ... Coverslips were washed twice for 30 min at 37°C with 2x SSC/10% formamide with 0.2 mg/ml DAPI (4’,6-Diamidin-2-phenylindol, Sigma) being added to the second wash ...
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bioRxiv - Biochemistry 2021Quote: ... with 10% ADC supplement (5% BSA, 0.85% NaCl, 2% dextrose) and 0.05% tyloxapol or 7H10-ADC agar medium (Sigma) at 37 °C ...
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bioRxiv - Neuroscience 2021Quote: ... rats were injected intraperitoneally with 100 mg/kg 5-Bromo-2′-deoxyuridine (BrdU, Sigma-Aldrich, St. Louis, MI) dissolved in warm 0.9% saline immediately after release from Decapicone bags ...
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bioRxiv - Neuroscience 2020Quote: ... (+)-Bicuculline (No. 14340) and DL-2-amino-5-phosphonopentanoinc acid (APV; No. A5282) were obtained from Sigma-Aldrich; 6,7-dinitroquinoxaline-2,3(1H,4H)-dione disodium salt (DNQX ...
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bioRxiv - Cancer Biology 2020Quote: ... Tissue was then incubated in CUBIC-1A solution (10% Triton, 5% NNNN-tetrakis (2-HP) ethylenediamine (Sigma, 122262), 10% Urea ...
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bioRxiv - Microbiology 2020Quote: ... PBMCs were stimulated with 50 U/ml IL-2 and 5◻μg/ml phytohemagglutinin (PHA; Sigma-Aldrich) for 72 h prior to infection or transduction ...
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bioRxiv - Microbiology 2021Quote: ... Cells were incubated with 0.1 mL serially diluted supernatant containing SARS-CoV-2 for 30 minutes at 37°C and 5% CO2 before being left in 1.5mL overlay (DMEM, 2% FBS, 1.2% cellulose microcrystalline (Sigma)) at 37°C and 5% CO2 for 72 hours ...
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bioRxiv - Neuroscience 2020Quote: ... After a 2 h blocking step in blocking buffer (PBX with 5% normal goat serum, Sigma-Aldrich, G9023) at RT ...
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bioRxiv - Immunology 2022Quote: ... in PBS 30 minutes at RT and blocked with 2% BSA and 5% Donkey Serum (Sigma-Aldrich, D9663) in PBS during 1 h at RT ...
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bioRxiv - Biochemistry 2022Quote: ... 5 mM MgCl2) for 2 min and then the DNA digested with 20U DNaseI (cat #: D4527, Sigma-Aldrich) in 1 mL of digestion buffer for 40 sec at RT ...
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bioRxiv - Genetics 2022Quote: ... Experiments for Figure 5 were as Figure 2 with the following changes: 10 μg/mL tunicamycin (InSolution, Sigma) was used ...
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bioRxiv - Cancer Biology 2022Quote: Cells were lysed using 5% SDS supplemented with Protease Inhibitor Cocktail and Phosphatase Inhibitor Cocktail 2 (Sigma-Aldrich). The following antibodies were purchased from Cell Signaling Technology (Danvers) ...
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bioRxiv - Immunology 2020Quote: ... Primary antibody in 250 μl of cold antibody buffer (20 mM HEPES-NaOH pH 7.5, 150 mM NaCl, 0.5 mM Spermidine, 2 mM EDTA, 0.1% digitonin, and protease inhibitor cocktails from Sigma) was added to the tubes and rotated at 4°C overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... slides were washed in PBS and incubated in 5 μg/ml 4’6-diamidino-2-phenylindole (DAPI) (Sigma Aldrich) in PBS (Sigma Aldrich ...
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Nanoscale molecular architecture controls calcium diffusion and ER replenishment in dendritic spinesbioRxiv - Neuroscience 2021Quote: ... and in addition of 5′fluoro-2-deoxyuridine + uridine (FUDR) (Sigma; 20 μg and 50 μg/ml, respectively), to block glial proliferation ...
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bioRxiv - Physiology 2022Quote: ... L4 staged worms were picked onto NGM plates containing 49.5 μM 5-fluoro-2’-deoxyuridine (FUDR, Sigma F0503), 50 μg/ml carbenicillin ...
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bioRxiv - Cell Biology 2020Quote: ... and Click-iT® EdU (5-ethynyl-2’deoxyuridine) Assay (BCK-EDU488, baseclick GmbH, Merck/Sigma-Aldrich, UK), respectively ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells in Accutase were added 1:2 to mTeSR™1 plus 5 μM Blebbistatin (Bleb, B0560, Sigma), pelleted at 150g for 5 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... Tissues were hybridized overnight at room temperature with the B-5-1-2 monoclonal anti-α-tubulin (Sigma) and the anti-KNOLLE antibody (kind gift of G ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoblots were carried out using the indicated antibodies: α-Tubulin (Sigma, B-5-1-2; T5168, 1:100,000), BLM (Bethyl ...
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bioRxiv - Cancer Biology 2023Quote: ... asynchronously growing subconfluent KB2P1.21 or KB2P3.4 cells were labeled with 30μM thymidine analogue 5-chloro-2’-deoxyuridine (CIdU) (#C6891, Sigma-Aldrich) for 20’ ...
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bioRxiv - Neuroscience 2023Quote: ... the larvae were anaesthetized with 0.02% Tricaine for 5-10 minutes and immobilized in 1.5-2% low-melting point agarose (Sigma) in fish water ...
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bioRxiv - Cell Biology 2023Quote: ... Tissues were incubated overnight at room temperature with the B-5-1-2 monoclonal anti-α-tubulin (Sigma) and the anti-KNOLLE antibody (kind gift of G ...
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bioRxiv - Microbiology 2022Quote: ... zebrafish embryos were treated with 180 µM of 1-phenyl 2-thiourea (PTU) (Sigma-Aldrich, 103-65-5), to prevent pigmentation [25] ...
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bioRxiv - Cancer Biology 2024Quote: ... β-estradiol was removed at day 5 from Type 2 medium and DMSO (Sigma, #D2650; final concentration = 0.1%) or 4-hydroxytamoxifen (Sigma ...
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bioRxiv - Developmental Biology 2023Quote: ... Tissues were hybridized overnight at room temperature with the B-5-1-2 monoclonal anti-α-tubulin (Sigma) and the anti-KNOLLE antibody65 (kind gift of G ...
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bioRxiv - Neuroscience 2023Quote: ... diluted in PBS + 5% NDS and in the second staining (2) 2B4 (1:200, Sigma Eldrich, Cat. # MAB5492), GFAP (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... Stock solutions of the NMDA receptor antagonist DL-2-amino-5-phosphonovaleric acid (DL-APV; #5282; Millipore Sigma), GABAA receptor antagonist bicuculline methiodide (#14343 ...
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bioRxiv - Cell Biology 2022Quote: ... reactions were incubated for 2 h on ice before quenching with 5 mM L-Cysteine (Millipore-Sigma, USA). The reactions were then adjusted to 1% (w/v ...