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Citations for Millipore Sigma :
3751 - 3800 of 10000+ citations for 6 fluoronaphthalene 1 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed with staining medium and re-suspended in 4’,6-diamidino-2-phenylindole (DAPI; 1 μg/ml; Sigma) to eliminate dead cells from sorts and analyses ...
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bioRxiv - Cancer Biology 2023Quote: Cells were seeded in 6 cm dishes one day prior to treatment with 1 μg/ml actinomycin D (Sigma-Aldrich) for the indicated time ...
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bioRxiv - Neuroscience 2023Quote: ... and plated at a density of 1-1.2x106 cells/ml onto 6-well plates treated with poly-D-lysine (EMD Millipore). Cells were cultured in Neurobasal A medium supplemented with 2% B27 ...
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bioRxiv - Neuroscience 2024Quote: ... The samples were then blocked in 6% donkey serum and immunolabeled for protein gene product 9.5 (PGP9.5, 1:500, Millipore-Sigma, SAB4503057-100UG) as a pan-neuronal marker or rabbit-IgG (Abcam ...
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bioRxiv - Molecular Biology 2024Quote: ... were treated with 50 µL of a 1mg/ml Concanavalin A (prepared in 10 mM sodium phosphate buffer pH 6, 10 mM CaCl2 ,1 mM MnCl2, 0.01% NaN3, Catalog C2010, Sigma Aldrich) solution ...
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bioRxiv - Cell Biology 2024Quote: ... samples were washed in 1X PBS and incubated with DAPI (4-6-diamidino-2-phenylindole, Sigma-Aldrich, 1 µg/mL) for 10 minutes at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... 125 µM amino acid standard solution was prepared by mixing physiological basis with acids and neutrals and internal standard solution (Sigma Aldrich Corp.). Data analysis was performed using the EZChrome software (Agilent Technologies) ...
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bioRxiv - Cell Biology 2021Quote: 1.5 µM ethylene glycol-bis(β-aminoethyl ether)-N,N,N’,N’-tetraacetic acid (EGTA) or 5 µM cyclopiazonic acid (CPA, CalBiochem-EMD Millipore). For Ca2+ replenishment ...
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bioRxiv - Cell Biology 2020Quote: ... in serum was analyzed by measuring 3-hydroxybutyric acid (BOH) and acetoacetic acid (AcAc) using Ketone body assay kit (Sigma, No. MAK134) according to manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2021Quote: The fatty acid- and retinol- binding preferences of Sc-FAR in vitro was measured by utilizing the saturated fatty acid fluorescent probe 11-(Dansylamino) undecanoic acid (DAUDA) (Sigma-Aldrich, USA) and retinol as previously described (Fairfax et al. ...
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bioRxiv - Biochemistry 2021Quote: ... The same procedure was conducted with gallic acid solutions with different concentrations prepared by diluting gallic acid monohydrate (Sigma-Aldrich, MO, USA) in distilled water ...
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bioRxiv - Immunology 2021Quote: ... One mL of supernatant was then transferred into a 1.5 mL centrifuge tube and mixed with 0.2 mL of metaphosphoric acid for deproteinizing and 0.1 mL of isocaproic acid as an internal standard (48.3 mM; Sigma-Aldrich, Saint Louis, MO). A standard curve was generated using five concentrations of acetate ...
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bioRxiv - Physiology 2024Quote: ... including chloride channel blockers DIDS (4,4’-Diisothiocyanato-2,2’-stilbenedisulfonic acid) and niflumic acid were purchased from Sigma-Aldrich (St. Louis, MO, USA). GSK1016790A (GSK101 ...
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bioRxiv - Microbiology 2022Quote: ... screw cap vials and 10 μL of a mixture of internal standards (20 mM of acetic acid-D4, Sigma-Aldrich #233315; propionic acid-D6, Sigma-Aldrich #490644 ...
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bioRxiv - Immunology 2023Quote: ... The combined ethyl acetate extracts containing free fatty acids or their derivatives were concentrated to ∼500 μL under a gentle nitrogen stream and applied to a small silicic acid column (2 × 90 mm containing 30 mg of Type 60A, 100–200 mesh silicic acid, Sigma-Aldrich Korea). Extracts were sequentially eluted with 250 μL of increasingly polar solvents starting with 100% ethyl acetate ...
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bioRxiv - Biochemistry 2023Quote: Tardigrade proteins were extracted using a modified acid extraction.35 Tardigrades were incubated in concentrated trifluoroacetic acid (Sigma Aldrich, St. Louis, MO) for 40 min before neutralizing to approximately pH 7 with 2 M Tris (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... without L-glutamine and without phenol red (Biochrom) supplemented with 30 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) (Sigma-Aldrich) with a pH of 7.4 ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were incubated in a modified reaction cocktail (209 µL PBS, 5 µL CuSO4, 25 µL 1 M L-ascorbic acid (Sigma, A2174), 1.25 µL AlexaFluor 488 dye ...
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bioRxiv - Cancer Biology 2020Quote: ... Reactions were terminated by spotting 15 µl onto P81 phosphocellulose paper (GE Lifesciences) and washing extensively in 1% phosphoric acid (Sigma-Aldrich). Radioactivity was quantified by liquid scintillation counting.
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bioRxiv - Microbiology 2019Quote: ... Each sample was then treated with 1% 3-(N,N-dimethyltetradecylammonio)propanesulphonate (Sigma-Aldrich; in 100 mM citric acid, pH 2.0) at 50°C for 20 min ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells equivalent to those present in 15 mL of the culture were added to 50 μL of the lysis buffer (PBST supplemented with complete and 1 mM PMSF) and 100 μL of acid-washed glass beads (150-212 μm; G1145; Sigma Aldrich). The cell lysates were bead-beaten using ShakeMaster® NEO (Bio Medical Science ...
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bioRxiv - Cell Biology 2020Quote: ... Osteogenic induction was performed in low-glucose DMEM medium supplemented with 50 μg mL−1 L-ascorbic acid 2-phosphate (Sigma-Aldrich), 10 mM glycerol 2-phosphate (Sigma-Aldrich) ...
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bioRxiv - Biophysics 2021Quote: ... spin labels were added to 200 µM final concentration into the same buffer containing 1- or 5 mM L-ascorbic acid (Sigma Aldrich).
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bioRxiv - Neuroscience 2021Quote: ... NTERA2 cells were trypsinized and plated at density 1×104 cells/cm2 in culture medium containing 10 μM all-trans retinoic acid (Sigma, R2625), the fresh medium was replaced every four days.
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bioRxiv - Neuroscience 2020Quote: ... All-trans retinoic acid (atRA) treatment was performed after recovering the acute slices by adding atRA (1 μM, Sigma Aldrich, #R2625) at a final concentration of 0.05% (v/v ...
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bioRxiv - Cell Biology 2022Quote: ... S-acylation labelling was then started by the addition of 50 μl of 600 μM C16:0-azide (final concentration 100 μM) diluted in serum-free DMEM supplemented with 1 mg/ml fatty-acid free BSA (Sigma, A7030). Cells were labelled for 4 h ...
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bioRxiv - Developmental Biology 2022Quote: ... and were cultured in 0.5× MBS +0.2% fatty acid free BSA (Fisher BP9704) + 50 μg/ml gent with the following concentrations of factors: 1 μM cycloheximide (CHX; Sigma C1988); 50 ng/ml BMP4 (R&D Systems 314-BP-010) ...
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bioRxiv - Developmental Biology 2021Quote: ... boiled in citric acid (pH 2.0) for antigen retrieval and stained with an α-MHC antibody (Sigma My-32, 1:1000) or α-GFP antibody (Abcam ab13970 ...
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bioRxiv - Neuroscience 2021Quote: 12 wells plates were coated o/n with poly-L-lysine (0.1 mg/ml [SigmaAldrich]) in 0.1 M borate buffer pH 8.5 (0.04 M Boric acid [Merck Millipore], 0.01 M Borax [SigmaAldrich]). 1 ml of plating medium (MEM Eagle’s with Earle’s BSS medium [Invitrogen] ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... is first coarsely emulsified in 22mL of a viscous aqueous buffer containing 10mM Sodium Dodecyl Sulfate (SDS) and 1 wt% alginate (Alginic acid sodium salt from brown algae, Sigma Aldrich). This crude emulsion is then injected into a narrow gap couette mixer ...
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bioRxiv - Plant Biology 2020Quote: ... For pipecolic acid treatment five-week-old plants were either watered with 10 ml water or 1 mM D/L-pipecolic acid (Pip; Sigma-Aldrich) solution as described before (Návarová et al. ...
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bioRxiv - Cancer Biology 2020Quote: One-dimensional aligned microfibers were created from a 21% (w/w) poly lactic-co-glycolic acid, PLGA (LACTEL) solution dissolved in hexafluoroisopropanol (HFIP, Krackeler) with 1% NaCl (Sigma Aldrich) and stirred overnight ...
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bioRxiv - Cell Biology 2021Quote: ... the 5’ sequence of the native promoter and amino acids 1-443 of HEH1 were amplified via PCR (KOD polymerase, EMD Millipore) from genomic DNA using primers encoding the restriction sites BamHI and HindIII ...
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bioRxiv - Immunology 2021Quote: ... TCA-fixed cells were stained by adding 50 μl of SRB solution 0.4% in 1% glacial acetic acid (Sigma, SRB #S1402) for 30 minutes at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... we treated 1 or 14 days primary cultures of hippocampal neurons O/N with 15 mM L-glutamic acid (LGA; Sigma-Aldrich). Total RNA concentration and purity (260/280 and 260/230 ratios ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... of finely ground plant material was extracted with 1ml of 70% MeOH with 1% formic acid (Fisher Chemical) as extraction buffer supplemented with 10ng/ml D4-Imidacloprid (Sigma-Aldrich) as an internal standard ...
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bioRxiv - Plant Biology 2022Quote: ... Over the course of 12 hours they were gradually dehydrated to 95% ethanol and then stained overnight in 1% Acid Fuchsin (Sigma-Aldrich) in 95% ethanol ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were washed twice with cold phosphate-buffer saline (PBS) and total proteins were precipitated in 5% trichloroacetic acid (TCA) with 1 mM Methionine (Met) (Sigma-Aldrich) for 15 minutes on ice ...
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bioRxiv - Molecular Biology 2023Quote: ... Slides were then incubated in AFOG staining solution (0.5% aniline blue, 1% orange-G, and 1.5% acid fuschin, pH 1.09; Sigma-Aldrich, 415049, O3756, F8129) for 7min at RT and then rinsed 1x for 2min with water ...
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bioRxiv - Molecular Biology 2023Quote: ... Slides were then washed 5-6x for 5 min with water and then rinsed in 1% Phosphomolybdic acid (Sigma-Aldrich, HT153) for 10 min and rinsed in water for 5 min ...
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bioRxiv - Plant Biology 2022Quote: ... 10 mg of freeze-dried pulverized leaves in liquid nitrogen were mixed with 1 mL of chilled solution containing 10% (w/v) trichloroacetic acid (TCA; Sigma-Aldrich) in acetone (Merck ...
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bioRxiv - Plant Biology 2023Quote: For phenotypic analyses single gemmae were placed on standard solid medium (control conditions) or medium supplemented with 10 μM 1-naphthylacetic acid (NAA) (1mg/ml, Sigma-Aldrich) or 10 μM 2-cis,4-trans-Abscisic acid (ABA ...
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bioRxiv - Immunology 2022Quote: ... sections were rinsed in 1% acetic acid followed by dehydration in increasing concentrations of ethanol and xylene and mounting using Entellan (Sigma-Aldrich). Images were acquired using a Leica DM750 microscope (Leica Microsystems ...
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Mitochondrial bioenergetics stimulates autophagy for pathological tau clearance in tauopathy neuronsbioRxiv - Neuroscience 2024Quote: ... isolated mitochondria equivalent to 1 mg protein were mixed with 250 μl methanol before adding 5 mL 0.1M Hydrochloric acid (Sigma, Cat# 1090601000) and 100 µL SPLASH (Avanti Polar Lipids ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were washed with A-RPMI and cultured from day 4 to day 8 in A-RPMI supplemented with 1 μM retinoic acid (Sigma R2625) and 100 ng/ml FGF2 ...
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bioRxiv - Immunology 2024Quote: ... U-937 cells were maintained in RPMI-1640 medium supplemented with 4.5 g/L glucose, 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, 1.0 mM sodium pyruvate (Sigma-Aldrich; Merck KGaA), 10% fetal bovine serum (Hyclone ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Pellet was resuspended into fresh buffer B and DNA was extracted using phenol-chloroform-isoamyl acid 25/24/1 (#77617; Sigma-Aldrich). After quantification with NanodropOne (Thermo-Fischer Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... cell pellets and synthetically prepared acyl-CoA standards were resuspended in 1 mL of ice-cold 10% trichloroacetic acid (Sigma-Aldrich). Samples and calibrators were spiked before extraction with 13C315N1-acyl-CoAs made biosynthetically in HepG2 cells serially passaged in stable isotope labeling from essential nutrients in cell culture using 13C315N1-pantothenate ...
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bioRxiv - Cancer Biology 2023Quote: ... The HLA complexes bound to the beads were then directly eluted into preconditioned Sep-Pak tC18 100 mg Sorbent 96-well plates (Waters, 186002321) using 1% trifluoroacetic acid (TFA; Sigma Aldrich). After two washes of 1mL 0.1% TFA ...
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bioRxiv - Microbiology 2023Quote: ... α- chymotrysin from bovine pancreas type II (C4129), 8-Anilino-1-naphthalenesulfonic acid (ANS, A1028) and concanamycin A (ConcA, C9705) were purchased from Sigma Aldrich. HBSS ...