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Citations for Millipore Sigma :
3701 - 3750 of 10000+ citations for 6 fluoronaphthalene 1 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... nuclear labelling was achieved by incubating the sections for 5 min in DAPI (4′,6-diamidino-2-phenylindole; 1:5000; Sigma). Two final washes in PBS were performed before the sections were mounted on glass slides using Vectashield (Vector Laboratories ...
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bioRxiv - Molecular Biology 2020Quote: ... the MILLIPLEX® MAP 6-plex Bcl-2 Family Apoptosis Panel 1 Magnetic Bead Kit (Millipore: Cat. No. 48-682MAG) was utilized ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were washed three times in PBS and mounted in 70% glycerol with with 5 μgml-1 concentration of 4-6-diamidino-2-phenylindole (DAPI, Sigma) and cells were mounted on cover slips ...
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bioRxiv - Microbiology 2021Quote: Plaque size analysis were performed in HEK293T Spir-1 WT and KO cells seeded in 6-well plates coated with poly-D-lysine (Sigma). Once confluent ...
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bioRxiv - Biochemistry 2021Quote: ... Protein extracts were incubated overnight with 1 μg of anti-PDGFRβ (D-6) antibody and immunocomplexes recovered using 25 µL Protein G Sepharose beads (Sigma) for 1 h 30 min in the wheel at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... diluted in 1% normal donkey serum in PBS with 2 µg/mL 4’,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich) for 1 h ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were released from the second thymidine block for 6 hours prior to the addition of Flavopiridol (1 μM; Sigma). After 1 hour ...
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bioRxiv - Cancer Biology 2020Quote: ... Lung tumors were induced in 6-8 weeks old mice by intraperitoneal injection of 8 doses of 1 g/kg of urethane (ethyl carbamate; Sigma); second dose was given 48h after the initial one and then once a week to reach a total of 8 doses ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and antigen retrieval was performed in 37 °C trypsin (pH 7.8) for 1 h or 10 mM sodium citrate buffer (pH 6) (Sigma-Aldrich) at 95 °C for 10 min ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were fixed using 6% formaldehyde/PBS solution for 10 minutes and stained with 1% crystal violet (Sigma Chemical Corp) in 70% methanol for 1 hour ...
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bioRxiv - Molecular Biology 2021Quote: ... The ionic liquid solution was dialyzed into lysis buffer (6 M urea, 2 M thiourea, 2% CHAPS, 1% DTT) using a 3k MWCO cellulose membrane (Amicon Ultra, Millipore).
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bioRxiv - Cell Biology 2022Quote: ... 0.005 % Tween20, 1 mg/mL BSA and the substrates NADP+ (20 μM, Cayman) and glucose 6-phosphate (G6P, 25 μM, Sigma) close to the KM ...
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bioRxiv - Neuroscience 2022Quote: ... the tissue and cells were incubated in 2-(4-amidinophenyl)-1H -indole-6-carboxamidine (DAPI, D-6564, Sigma, 1:1000). Biotinylated-secondary antibodies were from Vector Labs ...
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bioRxiv - Immunology 2022Quote: ... For cell sorting cell suspensions were stained for viability with 1:3000 4′,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich) immediately before sorting.
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bioRxiv - Neuroscience 2022Quote: Demyelinating lesions were induced in the spinal cord of 10-week-old female C57BL/6 mice by a stereotaxic injection of 0.5 μl of 1% LPC (Sigma-Aldrich) in sterile 0.9% NaCl solution ...
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bioRxiv - Developmental Biology 2022Quote: ... sections were washed in PBS and then incubated for 10 minutes in 1:10000 4’,6-diamidino-2-fenilindol (DAPI; Sigma) for nuclei counterstaining ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were cleared by centrifugation at 17,000 g 12 min and input aliquot was incubated with 6 mM MgCl2 and Benzonase (1:75, EMD Millipore). The remaining lysate was diluted with 300 μl of dilution buffer + supplements and incubated with 25 μl of GFP- or Myc-Trap magnetic agarose beads for 2 h at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Cortical neurons were plated in 6-well plates on coverslips coated with 100μg ml-1 poly-D-lysine (Sigma- Aldrich) and incubated at 37°C with 5% CO2 ...
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bioRxiv - Plant Biology 2021Quote: ... and the detection of the total MAPK3 and 6 protein levels were by using polyclonal anti-MAPK3 (1:1000, Sigma) and anti-MAPK6 (1:1000 ...
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bioRxiv - Immunology 2022Quote: ... The sections were washed in PBS and incubated 10 min with 4’,6-diamidino-2-phenylindole (DAPI, 1/10,000, Sigma-Aldrich) to visualize the cell nuclei ...
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bioRxiv - Cell Biology 2022Quote: ... Slides were moved to a humidified chamber with primary antibody (anti-acetyl-alpha tubulin, clone 6-11B-1, Sigma, MABT868) diluted in PBX (1:250 ...
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bioRxiv - Biochemistry 2019Quote: ... or doxycycline-treated (Dox) (1 µg/ml for 6 days) AW13516-shNPM1-luc+ cells using the GenElute Mammalian Total RNA Miniprep Kit (Sigma) followed by On-Column DNase I Digestion as per manufacturer’s protocol ...
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bioRxiv - Neuroscience 2019Quote: ... Picked spheres were plated (20 spheres per well of a 6-well plate) onto Nunclon-Δ plates coated with 0.1 mg mL−1 poly-L-ornithine (Sigma) followed by 10 µg mL−1 laminin (PO/Lam coating ...
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bioRxiv - Molecular Biology 2019Quote: ... All RNAs were then selectively modified with 10mM 1-methyl-6-nitroisatoic anhydride (1M6) (Sigma-Aldrich cat no. S888079-250MG) for 5 minutes at 37°C ...
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bioRxiv - Developmental Biology 2019Quote: ... Sections were further incubated for 10 min at room temperature with DAPI (4’,6-diamidino-2-phenylindole, 1:5000, Sigma). Incubation with the secondary antibodies lasted for 3h at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... To identify the total number of cells the nuclei were stained with DAPI (4’, 6-Diamino-2-phenylindole dihydrochloride) (1:10000, Millipore-Sigma). Images were acquired using Olympus BX61 microscope and analyzed using Fiji software (ImageJ).
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bioRxiv - Immunology 2019Quote: PBMCs (freshly isolated and previously frozen) and fresh whole blood were each stimulated for either 2 or 6 h with (1) PMA/ionomycin (Sigma) (PBMC ...
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bioRxiv - Physiology 2020Quote: ... and probed overnight (4 °C) with one of the following primary antibodies: Anti-TSG-6 mouse monoclonal (1 μg/ml; Millipore); rat monoclonal TNFα (1 μg/ml ...
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bioRxiv - Bioengineering 2021Quote: ... at −20°C for 6 minutes followed by a 1-hour incubation in blocking buffer containing 10 wt% BSA (Sigma) in PBS as previously described7 ...
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bioRxiv - Developmental Biology 2020Quote: ... The pregnant mice were serially scanned at E6.5 either after intra-peritoneal administration of 1µg/g BW of hyaluronan synthesis inhibitor 6-diazo-5-oxo-1-norleucine (DON, Sigma-Aldrich) at E3.5-E5.5 ...
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bioRxiv - Cell Biology 2020Quote: ... fixed accessory glands were permeabilised for 6 x 10 min in PBST (1 X PBS, 0.3% Triton X-100 [Sigma-Aldrich]), blocked for 30 min in PBSTG (PBST ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were washed with staining medium and re-suspended in 4’,6-diamidino-2-phenylindole (DAPI; 1 μg/ml; Sigma) to eliminate dead cells from sorts and analyses ...
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bioRxiv - Cell Biology 2020Quote: Total protein from confluent passage 1 Sirt5−/− and WT chondrocytes cultured in 6 cm dishes were collected in RIPA buffer containing 20 mM nicotinamide (Sigma) and protease inhibitors cocktail (ThermoFisher) ...
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bioRxiv - Microbiology 2021Quote: ... before cells were fixed with 1% formalin for 30 minutes and stained using Giemsa stain (SIGMA-ALDRICH®, 6% diluted) for 1 hour ...
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bioRxiv - Immunology 2020Quote: ... cells were resuspended in medium with 10% fetal calf serum and 1 × 105 cells were cultured for 4 to 6 days with different concentrations of ConA (Sigma) or co-cultured with (2.5 × 104 ...
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bioRxiv - Immunology 2020Quote: ... grown at the log-phase in 6-well plates in the presence of DMEM supplemented with 1% FBS and 0.5% (w/v) methylcellulose (Sigma-Aldrich). After 2 d of incubation at 37°C ...
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bioRxiv - Developmental Biology 2022Quote: ... 2/3 mTeSR1 + 1/3 N2 medium + LSX + PSD Day 6,7: 1/3 mTeSR1 + 2/3 N2 medium + PSD At day 6 cells were detached by Accutase solution (Sigma-Aldrich) and incubated at 37°C for 20 minutes to obtain a single cell suspension ...
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bioRxiv - Plant Biology 2022Quote: ... total glutathione in 0.1 ml of the supernatant was measured by spectrophotometry in a 1 ml mixture containing 6 mM DTNB (Sigma-Aldrich), 3 mM NADPH ...
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bioRxiv - Molecular Biology 2022Quote: ... the cells were washed several times in PBS and incubated with 1 µg/mL of 4’,6-diamidino-2-phenylindole (DAPI) (Sigma) to visualize the nuclei.
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bioRxiv - Molecular Biology 2022Quote: ... 500 mM NaCl and 6 mM β-mercaptoethanol) containing 1 mM phenylmethylsulfonyl fluoride (PMSF) and Protease inhibitor cocktail (Sigma-Aldrich) (20 mg/g of cell pellet) ...
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Neuroprotective Effects of VEGF-B in a Murine Model of Aggressive Neuronal Loss With Childhood OnsetbioRxiv - Neuroscience 2023Quote: ... in PBS for 2 h at room temperature and counterstained with 4’,6-diamidino-2-phenylindole (DAPI; 1:10,000; Sigma-Aldrich) to identify cell nuclei.
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bioRxiv - Immunology 2024Quote: ... slides were washed in PBS and incubated in 5 μg mL-1 4’,6-diamidino-2-phenylindole (DAPI) (Sigma Aldrich) in PBS (Sigma Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... The HeLa cells were transduced in 6-well plates with 1 mL of unconcentrated virus + 10 µg/mL polybrene (TR-1003-G, Sigma Millipore) per well for 24 h ...
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bioRxiv - Bioengineering 2024Quote: ... The slides were then washed with 0.1% Tris-buffered saline and Tween-20 (TBST) prior to 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI) staining (D9542 Sigma) for nuclei visualization ...
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bioRxiv - Cancer Biology 2023Quote: ... 3533-005-02) and overlaid with Organoid Media (Supplementary file 1) in 6-well ultra-low adhesion plates (Sigma, CLS3471). H2B:GFP retrovirus (91 ...
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bioRxiv - Neuroscience 2023Quote: ... HEK cells were washed three times with ice-cold PBSx1 and lysed using Maltoside lysis buffer [1% N-dodecyl maltoside (cat no. 69227-93-6 – Sigma) dissolved in PBSx1 with freshly added 0.1 mg/ml PMSF and protease inhibitors] ...
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Transport of metformin metabolites by guanidinium exporters of the Small Multidrug Resistance familybioRxiv - Biophysics 2023Quote: ... 100 mM KCl and pre-loaded with 0.4 mM substrate (Gdm+ or guanylurea) and 1mM pyranine (trisodium 8-hydroxypyrene-1, 3, 6-trisulfonate; Sigma-Aldrich) using three freeze/thaw cycles ...
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bioRxiv - Genomics 2023Quote: C57BL/6 mouse ESC were purchased from ATCC (SCRC1002) and maintained onto 1% gelatin-coated (Sigma-Aldrich, Cat. No G1393) dishes in DMEM knockout medium (Life Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... Monocytes were then isolated with CD14+ microbeads (MACS Miltenyi) and incubated for 6 days in RPMI/10%FBS and 1% penicillin/streptomycin solution (Sigma) supplemented with 50 ng/mL human GM-CSF (R&D Systems ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed with staining medium and re-suspended in staining media supplemented with 4’,6-diamidino-2-phenylindole (DAPI; 1 μg/ml; Sigma) to eliminate dead cells from analyses ...