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Citations for Millipore Sigma :
3701 - 3750 of 4506 citations for Recombinant Human CD274 Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: Overnight cultures were standardised to an OD600nm of 0.1 and incubated in 10% pooled human serum (Sigma Aldrich) diluted in PBS for 90 min at 37°C with shaking ...
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bioRxiv - Cell Biology 2021Quote: The digoxigenin-labeled antisense probe targeting bases 32-500 of human GNRH1 mRNA (NM_001083111.2) was transcribed in the presence of digoxigenin-11-UTP (Merck Millipore) in a reaction mixture containing linearized cDNA template (1 µg) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The antigen-antibody complexes were detected using peroxidase-conjugated goat anti-human or goat anti-mouse IgG (Sigma) and revealed using the enhanced chemiluminescence (ECL ...
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bioRxiv - Cell Biology 2021Quote: Human muscles were dissociated and digested with pronase enzyme (1.5mg/mL; protease from streptomyces griseus P5147-5G Sigma) in DMEM at 37°C for 20 min and passed through a 100 mm cell strainer (repeated 4 times) ...
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bioRxiv - Genomics 2021Quote: ... and VEGF) was measured in plasma using a non-human primate multiplexing bead immunoassay (Millipore-Sigma, Burlington, MA) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... and VEGF) was measured in plasma using a non-human primate multiplexing bead immunoassay (Millipore-Sigma, Burlington, MA) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: Human osteosarcoma U2OS cells (ATCC® HTB-96™) were cultivated in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma) with 10% fetal bovine serum (Lonza) ...
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bioRxiv - Developmental Biology 2021Quote: ... Human NTOs were permeabilized and blocked using .3% Triton X (PanREAC AppliChem) and 0.5% BSA solution (Sigma-Aldrich) for 30 m ...
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bioRxiv - Cell Biology 2020Quote: ... 25×106 cells were plated on 6-well culture dishes coated with human fibronectin (Sigma, St Louis, MO) and maintained in endothelial cell medium-2 (EGM-2 -Clonetics ...
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bioRxiv - Microbiology 2020Quote: ... Serum susceptibility was determined by incubation of late log-phase cells with 66 % normal human serum (Sigma-Aldrich) as described (Short et al ...
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bioRxiv - Cell Biology 2020Quote: ... rat anti-human RIPK3 (clone 1H2; produced in-house5 and available as Millipore MABC1640; 1:2000/1:400), rabbit anti-phospho-S227 human RIPK3 (Cell Signaling Technology ...
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bioRxiv - Molecular Biology 2022Quote: Human embryonic kidney (HEK) 293T cells (ATCC, CRL-3216) were cultured in DMEM (high glucose, Sigma-Aldrich, SH30022.01) with 10% FCS (Hyclone ...
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bioRxiv - Biochemistry 2022Quote: ... and human eEF1A1 (Horizon Discovery) was cloned into the NdeI and XhoI restriction sites of pET30a (Millipore Sigma). Quikchange mutagenesis (Agilent Technologies ...
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bioRxiv - Cancer Biology 2022Quote: ... The stability of the purified radioimmunoconjugate [225Ac]DOTA-YS5 (0.05 mL, 7.43 µCi) was verified by incubating with human serum (0.45 mL; Sigma Aldrich) and 0.9% Saline (0.45 mL ...
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bioRxiv - Microbiology 2022Quote: ... the apical channel was incubated with collagen IV (30 μg/mL) from human placenta (Sigma, cat. no. C7521) and collagen I (200 μg/mL ...
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bioRxiv - Microbiology 2022Quote: Pulmonary A549 adenocarcinoma human alveolar basal epithelial cell lines [13] were obtained from ECACC (#86012804, Sigma-Aldrich, Germany) and cultured in Roswell Park Memorial Institute medium (RPMI ...
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bioRxiv - Microbiology 2022Quote: ... 72) and diluted to OD600nm 0.1 in defined medium supplemented with 5% v/v human serum (Sigma-Aldrich). OD600nm was monitored at 3 ...
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bioRxiv - Microbiology 2022Quote: ... ubiquitin carboxyl-terminal esterase L1 (UCHL1) (29) using rabbit anti-human UCHL1 (Sigma, HPA005993 at 1:1,000 dilution). The secondary antibody was Alexa Fluor 594-conjugated goat anti-rabbit (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: Human embryonic kidney cells (HEK293T) were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Catalog no. D1152; Sigma-Aldrich) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2022Quote: ... 1 g/kg body weight) or human regular insulin solution (1 units/kg, catalog no. 19278; Sigma-Aldrich) was administrated intraperitoneally and blood glucose was measured from the tail vein at 0 ...
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bioRxiv - Molecular Biology 2022Quote: HeLa and human embryonic kidney (HEK) 293 cells were cultured in Dulbecco’s Modified Eagle’s medium (high glucose, Sigma) supplemented with 10% (v/v ...
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bioRxiv - Neuroscience 2022Quote: Control siRNA (siRNA scr) (siRNA Universal Negative Control #1 SIC001) and human GPC3 siRNA (NM_004484; SASI_Hs01_00205845) were purchased from Sigma-Aldrich and used at a concentration of 50 nM ...
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bioRxiv - Cell Biology 2024Quote: ... cover slips were incubated with the primary antibody (SV40 T antigen mouse-anti-human, Merck-Millipore, 1:50) overnight at 4 °C in a humidifier chamber ...
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bioRxiv - Cell Biology 2024Quote: ... the media was removed and monolayers fixed with methanol prior to antibody addition targeting human collagen I (Sigma) followed by AlexaFluor 488 secondary antibody (Life Technologies ...
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bioRxiv - Developmental Biology 2024Quote: ... Mouse embryos and human embryos were cultured in droplets of pre-conditioned KSOM (Sigma-Aldrich, MR-121-D) and GTL (Vitrolife ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were cultured using Fibronectin/Collagen/BSA ECM mixture-coated petri dishes (10µg/mL Human Fibronectin, Sigma Cat. No. F2006; 100µg/mL BSA, Sigma Cat.No.126575 ...
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bioRxiv - Immunology 2023Quote: ... AP-labeled goat anti-human IgG (γ-chain specific; Sigma-Aldrich, Vienna, Austria; 1:50,000 in TBS BSA) and AP-labeled goat anti-human IgA (α-chain specific ...
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bioRxiv - Microbiology 2023Quote: ... University of Lausanne) and Human Embryonic Kidney (HEK) 293T cells (ATCC) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM; Sigma) supplemented with 10% fetal bovine serum (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... 50mg fresh frozen postmortem human brain tissue was homogenized in Nuclei EZ Lysis buffer (NUC101-1KT, Sigma-Aldrich) and incubated for 5 min before being passed through a 70µm filter ...
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bioRxiv - Cell Biology 2023Quote: E0.5 embryos were cultured in embryo culture medium (EmbryoMax® Human Tubal Fluid, Sigma-Aldrich, # MR-070-D) under mineral oil for embryo culture (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... T cells were cultured in ImmunoCult-XV T cell expansion medium supplemented with human serum (10%; Sigma, USA), Penicillin-Streptomycin and IL-2 (100 IU/ml ...
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bioRxiv - Developmental Biology 2023Quote: ... Frozen-thawed human blastocysts (5-6 days post-fertilization) were gently treated by acidic Tyrode’s solution (Sigma, T1788) to remove the zona pellucida 4 ...
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bioRxiv - Microbiology 2023Quote: ... and human embryonic kidney (HEK) 293T or 293 cells were cultured in Dulbecco’s modified essential medium (DMEM) (Sigma) with 10% foetal calf serum (FCS ...
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bioRxiv - Pathology 2023Quote: Sub confluent human RPE cells were treated with 500 μM H2O2 (Sigma-Aldrich #H1009, Saint Louis, MO, USA) after sci control or siRNA transfection ...
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bioRxiv - Microbiology 2023Quote: ... Carbohydrate sources for the human fecal matter inoculated cultures were apple pectin (2.5 g/L, Sigma-Aldrich, USA) and porcine mucin (2.5 g/L ...
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bioRxiv - Bioengineering 2023Quote: ... we pre-treated 48-well plastic plates with 200 μL of 20 μg/mL human fibronectin (Milipore Sigma) for 10 minutes at 37 °C under 5% CO2 ...
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bioRxiv - Bioengineering 2023Quote: ... This suspension was incubated with an equal volume of 71.5 μg/mL AF647 anti-human VEGF antibody (Sigma) for 30 minutes at 37°C with gentle vortexing every 10 minutes before washing excess antibody with one high-dilution wash (>100x nanovial volume) ...
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bioRxiv - Cell Biology 2022Quote: esiRNAs specific to human caveolin-1 or ARHGAP29 and a non-targeting esiRNAs were purchased from Sigma-Aldrich and transfected at a final concentration of 50 nM using Dharmafect (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: Petrisoft™ easy coat hydrogel 0.2 kPa or 50 kPa 6-well plates were coated with 5μg/ml human fibronectin (Sigma) and RNA lysates harvested as described.
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bioRxiv - Genomics 2023Quote: Luminex assays were performed using the Milliplex Human Cytokine/Chemokine Magnetic Bead Panel (HCYTOMAG-60K-41, Merck Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... spermatozoa from JF1/MsJ males were capacitated in Human Tubal Fluid medium (Merck Millipore, Cat#MR-070-D) supplemented with 10 mg ml−1 Albumin (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... The cleavage fragments were analyzed by western blot with either mouse anti-human fibrinogen polyclonal antibody (Sigma-Aldrich) at a 1:5,000 dilution ...
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bioRxiv - Immunology 2023Quote: ... were coated overnight at 4 °C with 10 µg/ml of capture anti-human IgG (Sigma Aldrich, I2136) in PBS ...
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bioRxiv - Neuroscience 2023Quote: Human embryonic kidney (HEK) 293T cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM, Sigma, cat. no. D6429) supplemented with 10% heat-inactivated foetal bovine serum (hiFBS ...
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bioRxiv - Immunology 2022Quote: ... HRP-conjugated anti-mouse IgG Fab (Cat# A9917) and anti-human IgG Fab (Cat# SAB4200791) were from Sigma. HRP-conjugated anti-mouse IgG (Cat# PA1-28568 ...
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bioRxiv - Cell Biology 2024Quote: ... rat anti-human RIPK3 (clone 1H2; RRID:AB_2940816; 2g/L produced in-house (Petrie et al., 2019) and available from Millipore Cat# MABC1640) ...
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bioRxiv - Cell Biology 2024Quote: ... rat anti-human MLKL (clone 10C2; RRID:AB_2940821; 2g/L produced in-house (Samson et al., 2020) and available from Millipore Cat# MABC1635) ...
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bioRxiv - Cell Biology 2024Quote: ... rat anti-human MLKL (clone 7G2; RRID:AB_2940818; 2g/L produced in-house (Samson et al., 2020) and available from Millipore Cat# MABC1636) ...
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bioRxiv - Developmental Biology 2024Quote: ... spermatozoa were released into 500 μL of preincubated human tubal fluid medium (HTF: Millipore, Merck KGaA, Darmstadt, Germany) for 10 min at 37°C ...
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bioRxiv - Immunology 2023Quote: Human IAV strains were propagated in MDCK cells in the presence of 1 µg/ ml TPCK trypsin (Sigma) and harvested after 72 hours ...