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Citations for Millipore Sigma :
3651 - 3700 of 4506 citations for Recombinant Human CD274 Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2019Quote: ... tissue culture polystyrene culture plates were pre-coated with 20 mg/ml anti-IgG (human, Fc-specific, Sigma) for 1 hour at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... human HT-1080 fibrosarcoma cells (CCL-121; ATCC) were maintained using Eagle’s Minimum Essential Medium (EMEM; Millipore Sigma) supplemented with 2 mM L-glutamine and containing 10% (vol/vol ...
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bioRxiv - Pathology 2020Quote: ... and immunostained with human monoclonal anti-CRP antibody (C1688; Sigma-Aldrich, Saint Louis, MO, USA; 1:400 dilution) which specifically detect the 24-kD monomeric CRP epitope[31 ...
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bioRxiv - Genomics 2019Quote: ... we measured 41 different cytokines and chemokines using the Milliplex MAP Human Cytokine/Chemokine Magnetic Bead Panel (Millipore kit no ...
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bioRxiv - Biochemistry 2021Quote: ... reactions were then incubated in the presence of 50 μg/mL (~0.1 units) human salivary α-amylase (Sigma) or 1.5 M hydroxylamine for a further 60 min at 37°C ...
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bioRxiv - Genetics 2021Quote: ... AG1-0 cells were passaged according to the product manual for PluriSTEM Human ES/iPS Medium (Millipore Sigma), which is feeder-free and utilizes Dispase II (Millipore Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... Mouse monoclonal antibodies against human LMα3 (clone CL3112) and mouse IgG (both Sigma-Aldrich, St. Louis, Missouri, USA) were used at 0.5 μg mL−1.
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bioRxiv - Cancer Biology 2021Quote: ... and cultured in Human IntestiCult™ Organoid Growth Medium (StemCell) supplemented with 10 mmol/L Y-27632 (Sigma), 5 mmol/L CHIR99021 (ReproCell ...
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bioRxiv - Neuroscience 2021Quote: ... the eye cup was treated for ∼15 minutes with human plasmin (∼50 μg/mL, Sigma or Haematologic Technologies) to aid vitreous removal ...
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bioRxiv - Cell Biology 2019Quote: ... either directly or after coating with 10 µg/ml human plasma fibronectin (cat no. FC010; Millipore, Billerica, MA), as noted in the figure legend.
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bioRxiv - Biochemistry 2020Quote: DNA sequences containing human SUN2 were cloned into a modified bicistronic ampicillin resistant pETDuet-1 vector (EMD Millipore), superfolder GFP-KASH fusions (sfGFP ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were incubated with the respective primary antibody (rabbit anti human, anti-ANK1 or anti-EPB41, Sigma-Aldrich) overnight ...
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bioRxiv - Molecular Biology 2021Quote: Nuclei from human liver (15 mg) were isolated using with the Nuclei EZ Prep kit (Sigma-Aldrich, #NUC101) and a glass dounce homogenizer (25 strokes with lose pastel and 25 strokes with tight pastel in ice-cold Nuclei EZ Lysis Buffer) ...
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bioRxiv - Neuroscience 2020Quote: Chromatin for ChIP was prepared from immortalized human microglia using the Imprint® Chromatin Immunoprecipitation kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... shRNAs stably integrated in mouse and human cells were selected with 10 and 2 μg/ml puromycin (Sigma), respectively ...
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bioRxiv - Cancer Biology 2020Quote: ... Human colon cancer cell lines HCT116 TP53+/+ and HCT116 TP53-/- were grown in McCoy’s 5A Medium (Sigma, M9309) supplemented with 10% FBS and Pen/Strep ...
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bioRxiv - Microbiology 2020Quote: ... Bound antibody was detected with an anti-human antibody conjugated to horseradish peroxidase (HRP; EMD Millipore, Burlington, MA) and Ultra-TMB substrate (ThermoFisher) ...
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bioRxiv - Cell Biology 2021Quote: ... α-taxilin (NM_175852.4) and its truncated segments were amplified from human cDNA and cloned into pcDNA3.1(+) (Sigma-Aldrich). Full-length α-taxilin and γ-taxilin were also cloned into p3×FLAG-CMV-14 (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... HeLa D2 parental control cells were transfected with 300 ng/ml human VRK1 MISSION® esiRNA (EHU119541, Sigma) using HiPerFect Transfection Reagent (301707 ...
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bioRxiv - Microbiology 2021Quote: ... HMEC-1 cells (human microvascular endothelial cells RRID:CVCL_0307) were grown in HMEC media (MCDB 131 media (Sigma, M8537) supplemented with 10% FBS (HyClone ...
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bioRxiv - Microbiology 2021Quote: Calu-3 (human epithelial lung adenocarcinoma) cells were cultured in Minimum Essential Medium Eagle (MEM, Sigma, Cat#M4655) supplemented with 10% (upon and after viral infection ...
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bioRxiv - Microbiology 2021Quote: ... Then the inoculum was aspirated and DMEM supplemented with 5% NBCS and 7.5 µg/mL human immunoglobulin (Sigma) was added ...
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bioRxiv - Immunology 2021Quote: ... Serum IgG subclasses were tested with isotype-specific murine anti-human IgG subclasses (Sigma-Aldrich, S:t Louis, MA).
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bioRxiv - Microbiology 2021Quote: Calu-3 (human epithelial lung adenocarcinoma) cells were cultured in Minimum Essential Medium Eagle (MEM, Sigma, Cat#M4655) supplemented with 10% (upon and after viral infection ...
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bioRxiv - Microbiology 2021Quote: ... and differentiating cells for at least 7 days in RPMI supplemented with 10% Human AB Serum (Millipore Sigma), 100U/mL P/S ...
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bioRxiv - Microbiology 2021Quote: ... resolved by SDS–PAGE and analysed by immunoblotting using primary antibodies specific for human DDX42 (HPA023571, Sigma-Aldrich), Flag (M2 ...
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bioRxiv - Microbiology 2021Quote: ... Human HeLa cells (ATCC CCL-2) were maintained in Minimum Essential Medium Eagle (MEM) (Sigma-Aldrich, Taufkirchen, Germany), 7% FBS and 1% MEM non-essential amino acid solution ...
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bioRxiv - Immunology 2022Quote: Human THP-1 monocytes were differentiated into macrophages by 24 hr incubation with 100 nM PMA (Sigma-Aldrich) in complete RPMI medium at 1.6 × 105 cells/well in 12-well plates ...
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bioRxiv - Bioengineering 2022Quote: ... suspended in a solution prepared in the following order: 30 μL of human male AB serum (Sigma-Aldrich), 60 μL human plasma fibronectin (EMD Millipore) ...
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bioRxiv - Bioengineering 2022Quote: ... KCs were cultured and serially passaged into 7 μg/cm2 human collagen IV-coated plates (#C7521; Sigma-Aldrich). All cell sources were obtained at Yale-New Haven Hospital under protocols approved by the Yale University Institutional Review Board including ...
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bioRxiv - Biophysics 2022Quote: ... for mouse ILC2s and human peripheral ILC2s was supplemented with MEM nonessential amino acid solution (M7145, Sigma-Aldrich), 10 mM HEPES buffer (H3537 ...
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bioRxiv - Immunology 2022Quote: ... Human Umbilical Vein Endothelial Cells (HUVEC) were grown in 1% gelatin coated wells in M199 medium (Sigma-Aldrich) containing 20% fetal bovine serum (FBS) ...
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bioRxiv - Biochemistry 2020Quote: ... Human Rab8a 6-176aa (referred to as Rab8a)-encoding DNA was cloned into a pet51b(+) vector (Merck Millipore), resulting in a construct with a N-terminal Strep® II tag and enterokinase cleavage site and a C-terminal 10xHis tag ...
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bioRxiv - Developmental Biology 2021Quote: Human iPSCs were transduced in mTESR in the absence of antibiotics and with 6 μg/mL polybrene (Millipore). The lentiviruses containing the GOIs were transduced using 10 MOI in the presence of the reverse tetracycline transactivator M2rtTA (MOI 10) ...
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bioRxiv - Genomics 2019Quote: ... was cultured in Roswell Park Memorial Institute (RPMI) 1640 medium supplemented with 10% AB human serum (Sigma Aldrich), 100 U/mL recombinant human IL-2 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: Cell lines and isolated primary human immune cells were treated with etoposide (ETP, Sigma-Aldrich, cat. number E1383) at the indicated concentrations from a 50 mM stock that was prepared from a lyophilized powder reconstituted in DMSO (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... We detected bound antibody with horseradish peroxidase (HRP)-coupled anti-human (Fc) antibody (Sigma Aldrich catalog number A0170).
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bioRxiv - Cell Biology 2020Quote: Human monocyte-derived macrophages were separated from healthy donor buffy coats by centrifugation through a Histopaque 1077 (Sigma) gradient and adhesion purification ...
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bioRxiv - Immunology 2020Quote: ... Cell enrichment buffer consisted of 0.22 μm filtered PBS supplemented with 0.5% heat inactivated human AB serum (Sigma).
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bioRxiv - Biophysics 2021Quote: ... Samples were stained using a 1:100 dilution of the primary antibodies (rabbit polyclonal anti-human IRSp53, Sigma and rabbit polyclonal anti-human IRTKS antibody ...
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bioRxiv - Immunology 2021Quote: ... Cells were cultured in Human Plasma-like Media (HPLM) (8) + 1% pen/strep + 5% dialyzed serum (Sigma Aldrich). For SARS-CoV-2 peptide stimulation ...
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bioRxiv - Microbiology 2022Quote: ... The Madin Darby canine kidney (MDCK) and human embryonic kidney (HEK) 293T cells (ATCC) were maintained with Dulbecco’s Modified Eagle’s medium (DMEM) (Sigma), supplemented with 10% fetal calf sera (FCS ...
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bioRxiv - Immunology 2019Quote: ... Total antibody concentration between determinants was controlled by addition of human IgG2/kappa where needed (Sigma Aldrich; AG504). Cells were washed with ice-cold PBS and added to dengue immune complexes (MOI 5) ...
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bioRxiv - Cancer Biology 2020Quote: ... 10 mmol/L HEPES (PAA Laboratory) and 1 ng/mL human basic fibroblast growth factor (bFGF) (Sigma-Aldrich) (Lonza ...
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bioRxiv - Genomics 2020Quote: Human induced pluripotent stem cells (hiPSCs) were thawed with mTeSR1 (STEMCELL, #85850) and 1 μM H1152 (Millipore, #555550), seeded on Matrigel (Corning ...
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bioRxiv - Physiology 2021Quote: ... C2C12 myotubes were cultured in the differentiation medium containing 2 or 200 nM human insulin (Cat.# I9278, Sigma) for 16 hours prior to reaching day 10 (Fig.1A) ...
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bioRxiv - Neuroscience 2021Quote: Human embryonic kidney (HEK293) cells were maintained in a 37°C/5% CO2 atmosphere in DMEM:F12 (Sigma, D6421) supplemented with 10% foetal bovine serum (ClonTech ...
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bioRxiv - Immunology 2020Quote: ... For IgM antibody detection biotinylated anti-human IgM (affinity purified, μ-chain specific, Sigma-Aldrich cat. Nr. B1140) was used as secondary antibody.
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bioRxiv - Immunology 2020Quote: ... For IgM antibody detection biotinylated anti-human IgM (affinity purified, μ-chain specific, Sigma-Aldrich cat. Nr. B1140) was used as a secondary antibody ...
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bioRxiv - Cancer Biology 2019Quote: ... ECM proteins (collagen I and natural mouse laminin were purchased from Life Technologies, human plasma fibronectin from Millipore, human collagen IV from Sigma-Aldrich ...