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Citations for Millipore Sigma :
3701 - 3750 of 10000+ citations for 6 4 Methyl 1 piperazinyl N 5 methyl 1H pyrazol 3 yl 2 1Z 2 phenylethenyl 4 pyrimidinamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... and 2.5 μM 4-hydroxytamoxifen (4-OHT) (Sigma, Cat#H7904) to shuttle C/EBPα into the cell nucleus.
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bioRxiv - Cancer Biology 2022Quote: 10 mg/ml of 4-hydroxytamoxifen (4-OHT; Sigma, H6278) was dissolved in acetone with gentle heating ...
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bioRxiv - Neuroscience 2024Quote: ... 4-hydroxy-tamoxfen (4-OHT) was purchased from Sigma (H6728) and was dissolved in 100% DMSO and stored in the -20 until use ...
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bioRxiv - Neuroscience 2024Quote: Recombination was induced with 4-hydroxytamoxifen (4-OHT, Sigma-Aldrich), as previously reported [6,11] ...
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bioRxiv - Neuroscience 2023Quote: ... 4-methylumbelliferone (4-MU) standard (Sigma-Aldrich®; Cat# M1381) was included in 0 ...
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bioRxiv - Physiology 2023Quote: ... and 4-aminopyridine (4-AP) were obtained from Sigma-Aldrich. We used standard locust saline to prepare all pharmacological agents ...
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bioRxiv - Neuroscience 2023Quote: ... 10mg of 4-hydroxytamoxifen (4-OHT, Sigma Aldrich #H6278-10mg) was added with 500μl ethanol and shaken at room temperature until the powder was completely dissolved (20mg/ml) ...
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bioRxiv - Neuroscience 2023Quote: ... The 4-OHT (4-OHT; Sigma, MO, USA Cat# H6278) was dissolved in ethanol to create a 20mg/ml stock solution ...
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bioRxiv - Cancer Biology 2023Quote: ... 4-Methylumbelliferyl sulfate (4-MUS) substrate was from Sigma-Aldrich and GlcNS6S-GlcA-Glc6SNS-IdoA2S-GlcNS6S-IdoA2S-GlcNS6S-GlcA-pNP (2S2-6S4 ...
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bioRxiv - Cancer Biology 2023Quote: ... Tamoxifen (4-hydroxytamoxifen, 4-OHT) was obtained from Sigma (#H6278) and was reconstituted in dimethyl sulfoxide (DMSO ...
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bioRxiv - Neuroscience 2024Quote: ... followed by 4% paraformaldehyde (4% PFA; Sigma-Aldrich, cat# P6148) in 1x PBS pH 7.4 ...
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bioRxiv - Neuroscience 2022Quote: ... Endo-(±)-α-(Hydroxymethyl)benzeneacetic acid 8-methyl-8-azabicyclo[3.2.1]oct-3-yl ester (atropine) and 8-Cyclopentyl-1,3-dimethylxanthine (CPT) were from Sigma. Picrotoxin from Indofine Chemical Company (Hillsborough ...
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bioRxiv - Biophysics 2021Quote: NmeCas9 was expressed in Rosetta 2(DE3) cells (Millipore Sigma, 71400-3) and grown in LB supplemented with 100 µg/mL Carbenicillin and 34µg/mL Chloramphenicol ...
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bioRxiv - Neuroscience 2020Quote: ... containing Phosphatase Inhibitor Cocktails 2 and 3 (Sigma-Aldrich, St. Louis, MO) and MiniComplete Protease Inhibitor Cocktail (Roche Diagnostics) ...
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bioRxiv - Neuroscience 2022Quote: 2’-3’-dideoxycitidine (ddC) was purchased from Sigma Aldrich (St. Louis, MO). AM1710 was synthesized by the lab of Alexandros Makriyannis (Northeastern University ...
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bioRxiv - Cancer Biology 2022Quote: ... containing phosphatase inhibitor cocktails 2 and 3 and protease inhibitors (Sigma-Aldrich), for 30–45min at 4 °C ...
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bioRxiv - Plant Biology 2021Quote: ... 400μL (3 mg/mL) of methylbenzothiazolinone-2-hydrazone (MBTH, Sigma M8006−1G), 200μL of sample and 0.5μI of alcohol oxidase (E.C ...
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bioRxiv - Genetics 2020Quote: ... 137.14 mg of 2-methylpyridine-3-carboxylic acid (Sigma Aldrich, cat. 325228) were resuspended in 500 μl DMSO anhydrous (Sigma Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... and phosphatase inhibitors (cocktail 2; P5726; and cocktail 3; P0044; Sigma-Aldrich). Lysates were incubated for 5 min at 100 °C ...
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bioRxiv - Cell Biology 2022Quote: ... and phosphatase inhibitor cocktails 2 and 3 (Sigma-Aldrich, P5726 and P0044). Homogenized sample was left incubating for disulfide bond reduction for 60 min at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... and media was replaced every 2-3 days with MEME medium (Sigma), supplemented with 2 mM L-Glutamine (Gibco) ...
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bioRxiv - Microbiology 2024Quote: ... Mice were anesthetized by exposure to 2% to 3% isoflurane (Sigma-Aldrich) for 5 to 10 minutes and intranasally infected with 40 μL of a suspension containing 105 spores for the leukopenic model and 106 spores for the corticosteroid model ...
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bioRxiv - Cancer Biology 2023Quote: Spheroids were treated every 2-3 days with either GSK343 (Sigma-Aldrich), GSK126 (Selleckchem) ...
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bioRxiv - Cell Biology 2023Quote: ... 0.05 mM (1,4-di [3’-(2’-pyridyldithio)propionamido] butane) (DPDPB, Sigma 16646) in PBS for 5 min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... 2 and 3 inhibitor (Glutor, 0.05, 0.1, and 0.25 µM, Sigma, SML2765) for 6 to 72 hours ...
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bioRxiv - Neuroscience 2023Quote: 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium (MTT) (Millipore Sigma) is converted to water-insoluble product formazan by reduction at mitochondrial complex II of the electron transport chain ...
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bioRxiv - Biochemistry 2024Quote: ... exponentially growing TriEx cells (3 mL suspension, 2×106 cells/mL, Novagen) in serum-free Insect-XPRESS medium (Lonza ...
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bioRxiv - Plant Biology 2024Quote: ... different concentrations of pure 2-IPM and 3-IPM (Sigma-Aldrich, USA) ranging from 0.1 ng/ml to 1000 ng/ml in high-grade water were used (Supplementary Figure S5) ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were passaged every 2-3 days by trypsinization (Sigma-Aldrich, T4049) when density reached approximately 3.0×104 cells/cm2 (Mulas et al ...
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bioRxiv - Immunology 2024Quote: ... tips were silanized with 2% v/v 3-aminopropyltriethoxysilane (APTES, Sigma-Aldrich) in acetone for 10 min at room temperature (~22°C) ...
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bioRxiv - Immunology 2021Quote: ... antibody (1:1,200) (eBioscience) and for 5 min at 4 °C with the DAPI viability dye solution (1 µg/mL) (Sigma) to identify dead cells ...
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bioRxiv - Biochemistry 2022Quote: ... 4 mM DTT and 5% D2O at a protein concentration 1 mM using a Shigemi NMR tube (Sigma Aldrich). Data was collected on an ultrahigh field Bruker 900 MHz NMR spectrometer equipped with a triple resonance cryogenic probe ...
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bioRxiv - Developmental Biology 2023Quote: Membranes were incubated overnight in primary antibody o/n at 4°C: Rabbit ⍺-Vang (Rawls & Wolff, 2003) diluted 1:1,000 in 5% milk in TBST or Rabbit ⍺-Actin (Sigma) diluted 1:2,000 in 10% Seablock in TBST followed by 3x wash in TBST for 10 minutes each ...
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bioRxiv - Immunology 2023Quote: ... and digested in 4 mL of RPMI-1640/5% HI-FBS containing 1 mg/mL collagenase D (Sigma-Aldrich) and 30 μg/mL DNase I (Sigma ...
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bioRxiv - Immunology 2023Quote: Male mice were sensitized with 4 mg of PN butter (∼1 mg of protein; Capitán Mani) and 5 μg of cholera toxin (C8052, Sigma) in 0.5 mL of PBS administered intragastrically ...
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bioRxiv - Cell Biology 2020Quote: ... the wells were activated with N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride and N-Hydroxysuccinimide (both from Sigma-Aldrich) for 20 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... N-(3-aminopropyl)methacrylamide hydrochloride (APMA) and N,N′-bis(acryloyl)cystamine (BACA) were purchased from Sigma-Aldrich. Peptides ...
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bioRxiv - Genomics 2020Quote: Human melanoma cells were seeded in 12 well plates with increasing volumes of lentiviral supernatant in a final volume of 2 mL with 4 μg/mL polybrene (Millipore, Burlington, MA). Plates were centrifuged for 2 hours at 1,000x g at 30°C and cells were incubated for 16 hours at 37°C and 5% CO2 in a humidified incubator ...
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bioRxiv - Genomics 2020Quote: Human melanoma cells were seeded in 12 well plates with increasing volumes of lentiviral supernatant in a final volume of 2 mL with 4 μg/mL polybrene (Millipore, Burlington, MA). Plates were centrifuged for 2 hours at 1,000x g at 30°C and cells were incubated for 16 hours at 37°C and 5% CO2 in a humidified incubator ...
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bioRxiv - Microbiology 2020Quote: ... cultured for 24 h at 37°C with 5% CO2 and transduced with pseudoviruses harboring VSV-G or S-SARS-CoV-2 proteins or lacking the fusion protein (∆Env) in the presence of polybrene (4 µg/ml; Sigma-Aldrich, Poland) and HTCC-77 (100 μg/ml ...
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bioRxiv - Systems Biology 2022Quote: ... the pellicles were first digested by shaking for 16 h at 4 °C in 15 ml of PBS buffer with 2% cellulase (Sigma Aldrich, C2730).
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bioRxiv - Neuroscience 2021Quote: ... The supernatant became the soluble fraction and the pellet was solubilized in urea buffer (30 mM Tris, 7 M Urea, 2 M Thiourea, 4% CHAPS, 1X Protease Inhibitor Cocktail (Millipore Sigma 11873580001), 0.5 mM PMSF ...
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bioRxiv - Microbiology 2020Quote: Zn2+release from DksA was measured using the metal chelator 4-(2-pyridylazo)resorcinol (PAR) (Sigma-Aldrich, St. Louis, MO, United States) (59) ...
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bioRxiv - Biochemistry 2020Quote: ... Unbound dye was removed by 2 washes with 100X excess of protein storage buffer followed by centrifugation in Amicon® Ultra-4 Centrifugal Filter Units (SIGMA MilliPORE). LLPS of dyed and undyed protein was compared as quality control and results were similar (data not shown).
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bioRxiv - Molecular Biology 2020Quote: ... Table 2) and were introduced by 4 lentiviral infections at 12hr intervals in presence of 8 μg/ml polybrene (Sigma-Aldrich®) using supernatant from transfected 293T cells ...
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bioRxiv - Immunology 2022Quote: ... the retrovirus supernatant was concentrated by centrifugation at 5000g at 4 °C for 2 h with Amicon Ultra-15 Centrifugal Filter Units (Millipore, Billerica, MA). A human T cell line (Jurkat ...
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bioRxiv - Cell Biology 2022Quote: Cultured cells were fixed with 4% paraformaldehyde (PFA) and 2% sucrose and then stained with Nile red (Sigma-Aldrich, St. Louis, MO) and HCS Cell Mask Blue (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... EGTA-eluates were pooled and concentrated to a final concentration of ∼2 mg/ml using an Amicon Ultra-4 cellulose centrifugal filter unit (Merck Millipore, Cat# UFC810024).
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bioRxiv - Biochemistry 2022Quote: ... sodium hydroxide (1310-73-2) and α-amylase (DFP Treated, Type I-A, saline suspension, 647-015-00-4) were purchased from Sigma-Aldrich Company Ltd. ...
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bioRxiv - Cancer Biology 2024Quote: ... were coated overnight with retronectin solution (32 µg/mL in PBS, WEHI) at 4℃ followed by blocking with 2% bovine serum albumin solution (Sigma-Aldrich, #A1595) in PBS at 37℃ for 30 min prior to coating with viral supernatant ...