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Citations for Millipore Sigma :
3651 - 3700 of 10000+ citations for 6 4 Methyl 1 piperazinyl N 5 methyl 1H pyrazol 3 yl 2 1Z 2 phenylethenyl 4 pyrimidinamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... (2) 2% Alconox (SIGMA, 242985) (20 min sonication followed by 5X washes with MilliQ H2O) ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 mM 2-mercaptoethanol (Sigma). Proteasome Activity Fluorometric Assay II kit (UBPBio ...
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bioRxiv - Cell Biology 2021Quote: ... the mouse anti-alpha-tubulin B-5-1-2 was used at a dilution of 1:500 (Sigma, T5168). Polyclonal rabbit anti-TgCentrin1 (Fig ...
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bioRxiv - Molecular Biology 2019Quote: ... at a 1:2000 dilution while tubulin was incubated with anti-Tubulin antibody B-5-1-2 (Sigma-Aldrich) at a 1:1500 dilution ...
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bioRxiv - Developmental Biology 2023Quote: ... 2–3 endodermal explants (2PP or 3/4PP endoderm) were combined with 2–3 mesenchymal explants on Nucleopore membrane filters (Millipore) supported by fine meshed metal grids (Goodfellows) ...
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bioRxiv - Cell Biology 2020Quote: ... and Ypt1-7xHis were mixed in a 1:3 molar ratio and incubated overnight at 4°C with calf intestinal alkaline phosphatase (Sigma) to hydrolyze nucleotide ...
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bioRxiv - Molecular Biology 2020Quote: ... 4°C) twice with 300 μL of Elution buffer (Lysis buffer A with 1 mg/mL 3 × FLAG peptide (Sigma)) ...
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bioRxiv - Microbiology 2020Quote: ... Immunoprecipitation was performed at 4°C overnight with nutation in 1 ml chromatin aliquots with 3 ul of anti-FLAG M2 antibody (F3165, Sigma) and 20 ul of Protein G Dynabeads (Invitrogen) ...
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bioRxiv - Developmental Biology 2022Quote: ... Prior to start of imaging 6.5 ml culture medium with 1 μg/ml 17alpha,20beta-Dihydroxy-4-pregnen-3-one (DHP, diluted in 100% Ethanol, Sigma) containing drug/Lysotracker was added on top of the solidified LMP agarose ...
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bioRxiv - Microbiology 2024Quote: ... The presence or absence of bacteria in infected and uninfected samples was checked through staining with Phenolic Auramine O-Rhodamine B dye (4) (1/3 dilution of stock solution) (Auramine O-861020-25gm, Sigma) (Rhodamine B-R6626-100gm ...
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bioRxiv - Microbiology 2024Quote: ... The presence or absence of bacteria in infected and uninfected samples was checked through staining with Phenolic Auramine O-Rhodamine B dye (4) (1/3 dilution of stock solution) (Auramine O-861020-25gm, Sigma) (Rhodamine B-R6626-100gm, Sigma). Coverslips were mounted on glass slides and visualized using CLSM with a 488 nm laser.
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bioRxiv - Physiology 2023Quote: ... Following overnight incubation at 4°C with primary antibodies against CARNS1 (rabbit polyclonal, 1:1000 in 3% milk/TBS-T, HPA038569, Sigma), membranes were washed (3 × 5 min) ...
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bioRxiv - Biochemistry 2022Quote: ... Purified proMMP-3 and mutants were activated for 16 h in the presence of 1 mM 4-amino-phenyl mercuric acetate (Sigma) at 37 °C ...
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bioRxiv - Biophysics 2023Quote: ... and the remaining solution containing the hydrogel microspheres was supplemented with 1 mL of 20% v/v of PFO (1H,1H,2H,2H-perfluoro-1-octanol 97 %, Sigma-Aldrich) in Novec 7500 for 3 h to remove the surfactant from the droplets ...
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bioRxiv - Biophysics 2023Quote: ... the oil phase was carefully removed with a pipette tip and replaced with 1 mL of 20% v/v of PFO (1H,1H,2H,2H-perfluoro-1-octanol 97 %, Sigma-Aldrich) in Novec 7500 oil for 3 hr to remove the surfactant from the microspheres ...
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bioRxiv - Developmental Biology 2024Quote: Adult zebrafish were injected intraperitoneally at 2 dpci with 20 μl of 2.5 mg/ml of 5-Bromo-2-deoxyuridine (BrdU, B5002-1G, Sigma). Livers were collected and processed for analysis at 5 dpci ...
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bioRxiv - Systems Biology 2020Quote: ... for 1 – 4 hours at 4°C and subjected to IP overnight at 4C (0.3 µg H3K27me3 Millipore 07-449 ...
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bioRxiv - Cell Biology 2021Quote: ... THP-1 cells were treated for 30 min at 4°C in fixation buffer (4% formaldehyde (Sigma #47608)/5 mM EDTA ...
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bioRxiv - Molecular Biology 2019Quote: ... 4-HNE protein adducts were detected by an anti 4-HNE protein adduct antibody (1:2000, AB5605, Millipore) in Tris-buffer saline with 5% skim milk ...
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bioRxiv - Genomics 2024Quote: ... samples were embedded in a thin polyacrylamide film by inverting them onto a GelSlick-coated microscope slide with a droplet of 4% acrylamide solution (4% v/v 20:1 acrylamide:bis-acrylamide [BioRad, 1610144] with 0.15% v/v TEMED [Sigma, T7024] and 0.30% v/v ammonium persulfate [Sigma ...
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bioRxiv - Microbiology 2021Quote: ... Bacillus cultures were adjusted to 2*10−2 or 8*10−4 at OD600 and 5-fold dilution series were prepared with 6 steps using peptone saline as diluent ([Millipore]; Maximum Recovery Diluent 9.5 g/L). 15 μl of each bacterial dilution was inoculated in consecutive wells by spotting the solution in the center of the wells ...
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bioRxiv - Plant Biology 2020Quote: Three-week-old tomato plants were placed under either WL or WL+FR for five days and were sprayed daily with a 50 μM methyl jasmonate (MeJA, Sigma-Aldrich®) or mock solution (0.1 % EtOH ...
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bioRxiv - Immunology 2020Quote: ... The bound proteins were washed with PBS and then eluted with 50 mL of 1M Methyl α-D-mannopyranoside (Sigma M6882-500G) in PBS ...
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bioRxiv - Bioengineering 2022Quote: Cellulose scaffolds (Miniminit Products, R10, Scarborough, Canada) were partially infiltrated with a poly(methyl methacrylate) (PMMA, 120KDa, Sigma-Aldrich, St. Louis, USA) solution in acetone (Sigma-Aldrich ...
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Sex-specific fear acquisition following early life stress is linked to amygdala glutamate metabolismbioRxiv - Animal Behavior and Cognition 2024Quote: To extract polar metabolites from mouse (BLA tissue samples, a sample extraction buffer consisting of methyl tert-butyl ether (MTBE, Sigma-Aldrich, 650560), methanol (Carl Roth ...
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bioRxiv - Immunology 2023Quote: ... Tumour cells were cultured in 30 µl droplets containing 4000 tumour cells resuspended in spheroid medium (60 % growth medium and 40 % low viscosity methyl cellulose medium (25cp viscosity; Sigma Life Science), to which 3.6 µl of PureCol Type I Collagen (3.1 mg/ml stock solution ...
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bioRxiv - Biophysics 2023Quote: ... delipidation solutions for 1–2 days at 37°C in a shaker, tB-PEG (containing 70% tB, 27% (v/v) poly (ethylene glycol) methyl ether methacrylate average Mn500 (Sigma-Aldrich, 447943) and 3% (w/v ...
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bioRxiv - Bioengineering 2024Quote: Polymer sheets were obtained by radical polymerization of a solution of either MA (methyl acrylate) or EA (ethyl acrylate) (Sigma-Aldrich, UK), using 1 and 0.35 weight percent benzoin (98% pure ...
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bioRxiv - Neuroscience 2020Quote: ... Trim28-fl(+/− and +/+) animals (Ctl n=5, KO n=5) were put in RIPA buffer (Sigma-Aldrich) containing Protease inhibitor cocktail (PIC ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.2 mg/mL sheared salmon sperm DNA and 2 mg/mL BSA) containing 50 ng of or HLA-B-biotin DNA probe (5’ TGTCCTAGCAGTTGTGGTCATCGGAGCTGTGGTCGCTGCTGTGAT-biotin 3’) (Sigma-Aldrich, USA) in a humidified chamber overnight ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were washed 3 times with 1x PBS and incubated for 2 hours with 5% goat serum (NGS -Sigma-Aldrich, G9023) in 1x PBS with 0.3% Triton (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... The locus with expected insertion was amplified by PCR using 5 μL KOD Hot Start 2× Master Mix (Sigma Aldrich 71842-3), 0.2 μL each primer at 10 μM ...
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bioRxiv - Molecular Biology 2021Quote: ... 2-Deoxy-D-glucose (2-DG; 2 mM, Sigma-Aldrich).
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bioRxiv - Biochemistry 2024Quote: ... 2 mM 2-Mercaptoethanol (Sigma-Aldrich; Cas: 60-24-2), and 4 mM 4-Nitrophenyl acetate (Sigma-Aldrich ...
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Genomic organization of the autonomous regulatory domain of eyeless locus in Drosophila melanogasterbioRxiv - Genetics 2021Quote: ... and finally stained for 4-6 h in lacZ staining solution with 0.2% X-gal (Sigma). The stained embryos were imaged in Leica stereomicroscope ...
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bioRxiv - Cell Biology 2019Quote: ... cells were plated on 4-6 18 mm glass coverslips coated with fibronectin (50 nM, Sigma) and equilibrated in culture medium ...
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bioRxiv - Neuroscience 2019Quote: ... dissociated tissues were plated at 4 million cells/6 cm poly-D-lysine-coated plate (Sigma) grown in Neurobasal Media supplemented with B27 (Gibco ...
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bioRxiv - Biochemistry 2024Quote: ... Fractions were digested at 37 °C for 4-6 hours with 500 ng of trypsin (Sigma), chymotrypsin (Thermo) ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were ground in an ice-cold buffer (50 mmol.L-1 TRIS, 5 mmol.L-1 EDTA, 2 mmol.L-1 DTT, protease inhibitor cocktail (Sigma, St. Louis, MO, USA)) ...
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bioRxiv - Immunology 2019Quote: ... 4-5 week old mice received at two consecutive days tamoxifen (T5648, Sigma, dissolved in 1:9 ethanol:corn oil (C-8267 ...
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bioRxiv - Bioengineering 2023Quote: ... Lyophilized PEG-OH (5 g) and 4-Dimethylaminopyridine (DMAP, 0.002 mol; Sigma-Aldrich) were dissolved in anhydrous dichloromethane (DCM ...
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bioRxiv - Microbiology 2023Quote: ... non-metabolizable 4 kDa FITC-labeled dextran (FD4; 5 mg/ml, Sigma-Aldrich) was added with bacteria (MOI ...
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bioRxiv - Immunology 2024Quote: ... 4×105 cells were incubated with 5 µg/ml of Hoechst 33342 (Sigma) at 37°C for 45 min ...
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bioRxiv - Cancer Biology 2019Quote: ... 107 cells cultured with 100nM 4-OHT or ethanol control for 6 or 24 hours were incubated with 1% formaldehyde (Sigma-Aldrich, F8775) at room temperature for 10 min ...
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bioRxiv - Systems Biology 2023Quote: ... K562 cells were transduced in 6-well plates by mixing the cells and virus with 1:500 dilution of 4 mg/mL Polybrene (Sigma H9268-5G). For spinfection ...
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bioRxiv - Cell Biology 2022Quote: ... Treatment with 4-hydroxytamoxifen (4-OHT, Sigma-Aldrich cat #H7904) and Shield-1 (Cheminpharma cat #CIP-S1-0005 ...
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bioRxiv - Neuroscience 2021Quote: ... 4-Hydroxytamoxifen (4-HOT) (H-7904, Sigma, St. Louis, MO), the active metabolite of tamoxifen ...
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bioRxiv - Synthetic Biology 2020Quote: ... (Z)-4- hydroxytamoxifen (4-HT, H7904, Sigma-Aldrich, 10 mM), 3,3’,5-triiodo-L-thyronine (T3 ...
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bioRxiv - Neuroscience 2019Quote: ... 4-Methylumbelliferyl β-D-glucopyranoside (4-MU) GBA substrate (Sigma).
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bioRxiv - Neuroscience 2019Quote: ... and 100 µM 4-Aminopyridine (4-AP, #275875, Sigma Aldrich). GluR inhibition was performed using 40 µM D(-)-2-amino-5-phosphonopentanoic acid (APV ...