Labshake search
Citations for Millipore Sigma :
3651 - 3700 of 8516 citations for V Bottom Microplate 96 well since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2021Quote: ... and 3% w/v Quadrol) for 2 days with daily change for delipidation and BB-PEG (75% v/v benzyl benzoate (BB) (W213802, Sigma-Aldrich), 22% v/v PEGMMA500 and 3% w/v Quadrol) ...
-
bioRxiv - Plant Biology 2022Quote: ... Seeds were sterilized with (0.05% (v/v) Tween-20 solution for 10 minutes) and subsequently sown on 1/2 Murashige and Skoog (MS) medium (Sigma M5519) at 22□ in long-day conditions (16 h light/8 h dark) ...
-
bioRxiv - Pathology 2021Quote: ... The tissue was blocked for 1 hour at room temperature with 1x TBS IHC Wash Buffer with Tween 20 with 3% (v/v) normal donkey serum (Sigma-Aldrich), 0.1% (v/v ...
-
bioRxiv - Immunology 2021Quote: ... PBMCs were isolated from venous blood samples via density gradient centrifugation according to standard protocols and cryopreserved in complete medium supplemented with dimethyl sulfoxide (DMSO; 10% v/v; Sigma-Aldrich) and fetal calf serum (FCS ...
-
bioRxiv - Microbiology 2020Quote: ... The heated samples were subjected to 12% (v/v) SDS-PAGE and then electrophoretically transferred onto a nitrocellulose membrane (320mA for 2h) (Sigma-Aldrich) in transfer buffer [25mM Tris–HCl pH 7.5 ...
-
bioRxiv - Molecular Biology 2020Quote: ... and 0.05% (v/v) of an antibiotic and antimycotic mixture (penicillin, amphotericin B, and streptomycin; Sigma Aldrich, St. Louis, MO, USA)5 ...
-
bioRxiv - Cell Biology 2021Quote: ... glass slides were subjected to Prussian Blue staining using a 1:1 solution (v/v) of 10% K4[Fe(CN)6] (Sigma-Aldrich) and 20% HCl for 20 min ...
-
bioRxiv - Microbiology 2021Quote: ... HeLa 229 cells (ATCC® CCL-2.1™) and HeLa 229 pInducer11 shc-Myc cells were cultured in 10% (v/v) heat inactivated FBS (Sigma-Aldrich) RPMI1640 + GlutaMAX™ medium (Gibco™) ...
-
bioRxiv - Immunology 2021Quote: ... Both antibodies are biotinylated and were therefore detected with a streptavidin-phycoertyhrin (SA-PE) conjugate solution (diluted 1:1000 in a 1% w/v BSA fraction V (Sigma-Aldrich)/PBS solution) ...
-
bioRxiv - Plant Biology 2022Quote: ... seedlings were grown vertically on half-strength MS plates supplemented with 1% (w/v) sucrose and 0.5% (w/v) Phytagel (Sigma-Aldrich, P8169). Five-day-old seedlings were moved to liquid half-strength MS medium containing 2 μM synthetic RALF1 peptide (Sangon Biotech Co ...
-
bioRxiv - Microbiology 2023Quote: ... Stock cultures were stored at -80°C in cryogenic vials by resuspension of fresh growth in Tryptic Soya Broth (TSB; Oxoid) containing 8% (v/v) dimethyl sulfoxide (Sigma Aldrich). Culture purity was determined by plating frozen stocks onto Tryptic Soy Agar (TSA ...
-
bioRxiv - Neuroscience 2022Quote: ... The fixed optic nerves were dehydrated in incremental concentrations of tetrahydrofuran (THF; 50%, 80%, 100%, and 100%, %v/v in distilled water, 20 min each; Sigma-Aldrich) in amber glass bottles on an orbital shaker at room temperature ...
-
bioRxiv - Biochemistry 2022Quote: ... 100% ethanol was added to the proteome digest to a final concentration of 40% v/v before addition of undecanal (EMD Millipore) at an undecanal to peptide ratio of 20:1 w/w and addition of 1 M sodium cyanoborohydride to a final concentration of 30 mM ...
-
bioRxiv - Cancer Biology 2024Quote: ... Membranes were blocked with PBS containing 0.1% (v/v) Tween-20 and either 5% (w/v) non-fat dry milk (Carnation) or 3% bovine serum albumin (BSA, Sigma-Aldrich) for phosphorylated proteins detection ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... was electrophoresed at 170 V for 50 min and transferred at 20 V for 2 hours to PVDF membranes (EMD Millipore). Membranes were blocked with 5% non-fat milk at room temperature for 1 hour and incubated with primary antibodies (see reagents) ...
-
bioRxiv - Neuroscience 2022Quote: ... dehydrated in an ascending series of tetrahydrofuran (50%, 70%, 80%, 100% and 100%, v/v in distilled water, 20 min each, Sigma-Aldrich), and cleared in a 1:2 mixed solution of benzyl alcohol and benzyl benzoate (BABB ...
-
bioRxiv - Neuroscience 2022Quote: ... rinsed slices were incubated in 10 mM sodium citrate (pH 6.0) with 0.05 % Tween-20 (v/v, Sigma Aldrich, Taufkirchen, Germany) for 20 min at RT ...
-
bioRxiv - Immunology 2022Quote: ... Tissues were then washed with wash buffer and blocked for 1 h at room temperature with 1x PBS IHC Wash Buffer with Tween 20 and 5% (v/v) normal donkey serum (Sigma-Aldrich). Two antibody panels were prepared ...
-
bioRxiv - Immunology 2022Quote: ... The first antibody cocktail was prepared in 1x PBS IHC Wash Buffer with Tween 20 with 5% (v/v) normal donkey serum (Sigma-Aldrich) and 0.5 mM EDTA (Sigma Aldrich ...
-
bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10% (v/v) fetal bovine serum (FBS) and 1X antibiotic-antimycotic solution [DMEM and 1X antibiotic-antimycotic solution from Sigma-Aldrich, St ...
-
bioRxiv - Immunology 2024Quote: Tissues were then washed with wash buffer and blocked for 11 hours at RT with 1X TBS with 5% (v/v) normal donkey serum (Sigma-Aldrich). The first antibody cocktail was prepared in 1X TBS-T 5% (v/v ...
-
bioRxiv - Cell Biology 2023Quote: ... Proteins were separated by 10% SDS-PAGE (90 V, 30 min; 120 V, 1 h) and transferred to a Polyvinylidene Fluoride (PVDF, Millipore, IPVH00010) membrane (290 mA ...
-
bioRxiv - Immunology 2023Quote: ... These PBMCs were then re-suspended in large volumes of RPMI 1640 medium containing 5% v/v fetal calf serum (FCS) (F-0926, Sigma, USA) and centrifuged against at 500 x g and after a further wash their PMBCs were re-suspended in ice-cold 10% dimethyl sulfoxide (D-2650 ...
-
bioRxiv - Cell Biology 2023Quote: ... Sections were collected and deposited on formvar- and carbon-coated grids using 2.3 M sucrose and 1.8 % (v/v) methylcellulose (MC; Sigma M6385 25 centipoises) mixed 1:1.
-
bioRxiv - Synthetic Biology 2023Quote: ... A whole colony was picked from this plate and inoculated into a preculture of 10mL HS media with 0.2% v/v cellulase (Sigma Aldrich C2730) in a 125mL flask ...
-
bioRxiv - Systems Biology 2023Quote: ... The first antibody cocktail was prepared in 1x TBS IHC Wash Buffer with Tween 20 with 3% (v/v) normal donkey serum (Sigma-Aldrich) and filtered through a 0.1 μm centrifugal filter (Millipore ...
-
bioRxiv - Systems Biology 2023Quote: ... Tissues were then washed with wash buffer and blocked for 1 hour at room temperature with 1x TBS IHC Wash Buffer with Tween 20 with 3% (v/v) normal donkey serum (Sigma-Aldrich), 0.1% (v/v ...
-
bioRxiv - Cancer Biology 2023Quote: ... They were then incubated in primary antibodies (supplementary table 7) diluted in Odyssey blocking buffer supplemented with 0.2% (v/v) Tween 20 (Sigma-Aldrich, P9416) overnight at 4°C ...
-
bioRxiv - Microbiology 2022Quote: ... was dissolved in 250 μl of hexadeutero-dimethylsulfoxide (DMSO-d6) containing 1% (v/v) of tetramethylsilane (TMS) as a reference (Sigma-Aldrich), and transferred in a 3 mm NMR tube (103.5 mm long ...
-
bioRxiv - Microbiology 2023Quote: ... was added for 1 hour at room temperature and quenched with 8 µl of 5% (v/v) of hydroxylamine in water (Sigma Aldrich). 1 μl of 200 mM TCEP was added for 1 hour at 55 °C and free thiol groups were alkylated with 1 μl of 380 mM Iodoacetamide (Thermo Fisher ...
-
bioRxiv - Systems Biology 2022Quote: ... supplied in 1 μL of digestion buffer (100 mM TEAB pH 8.5, 1:5000 (v/v) benzonase (Sigma cat. nr. E1014)) ...
-
bioRxiv - Microbiology 2023Quote: Neutrophils were recovered from bone marrow suspensions following double-gradient centrifugation (Histopaque-1119, Histopaque-1077: 50/50 v/v; SIGMA, USA). Cells (1 × 105 cells/mL ...
-
bioRxiv - Cancer Biology 2023Quote: ... and 1% (v/v) Penicillin-Streptomycin (Pen-Strep, 100 U mL−1 and 100 μg mL−1 respectively, Sigma-Aldrich, #P4333). 3T3-L1 cells were cultured routinely in DMEM supplemented with 10% (v/v ...
-
bioRxiv - Microbiology 2023Quote: ... at 15 days and 36 days with 1:1 (v/v) mixture of antigen with incomplete Freund’s adjuvant (Sigma-Aldrich, USA). After the first immunization ...
-
bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked for 1 hour at room temperature in 5% (w/v) non-fat milk (Marvel) or 5% (w/v) BSA (Sigma-Aldrich) depending on the antibody specifications ...
-
bioRxiv - Cancer Biology 2023Quote: ... washed with complete T cell medium supplemented with 1% (v/v) FBS and then TCR cross-linked with goat α-hamster IgG (20 μg /ml, Sigma) for the indicated times at 37°C ...
-
bioRxiv - Biophysics 2023Quote: ... Glass coverslips were plasma activated and incubated with a solution of 2% (v/v) 3-(trimethoxysilyl)propyl methacrylate (440159-500ML, Sigma Aldrich) and 1% (v/v ...
-
bioRxiv - Bioengineering 2023Quote: ... NF 350 mineral oil with a viscosity of 57.2 mPa.s and density of 857 kg/m3 was used as the continuous fluid and 5% V/V Span-80 surfactant (Sigma-Aldrich) was added to the oil to stabilize droplets ...
-
bioRxiv - Cell Biology 2023Quote: ... were cultured at 28°C in SDM-79 media supplemented with 10% v/v heat-inactivated fetal calf serum (Sigma-Aldrich) (Brun and Schönenberger ...
-
bioRxiv - Neuroscience 2023Quote: Fixed optic nerves were incubated in stepwise concentrations of tetrahydrofuran (TFH) (steps: 50%, 70%, 80%, 100%, and 100%, v/v % in distilled water, Sigma-Aldrich) in a twelve-well glass plate to dehydrate the nerves ...
-
bioRxiv - Molecular Biology 2023Quote: ... The resulting concentrate was applied to a 10 - 50% v/v iodixanol step gradient with 10% increments (OptiPrep; Sigma Aldrich, Merck) enriched with sea salts (Sigma Aldrich ...
-
bioRxiv - Biophysics 2023Quote: ... and the remaining solution containing the hydrogel microspheres was supplemented with 1 mL of 20% v/v of PFO (1H,1H,2H,2H-perfluoro-1-octanol 97 %, Sigma-Aldrich) in Novec 7500 for 3 h to remove the surfactant from the droplets ...
-
bioRxiv - Biophysics 2023Quote: ... phosphoenol pyruvate (53 mM PEP, Beantown Chemical, 129745) and pyruvate kinase/lactate dehydrogenase enzymes (2.8% v/v PK/LDH, Sigma, P-0294). For experiments with PRC1 ...
-
bioRxiv - Bioengineering 2023Quote: ... followed by 1-hour blocking of non-specific binding sites with PBS with 5%v/v bovine serum albumin (BSA) (Sigma Aldrich) at room temperature ...
-
bioRxiv - Biochemistry 2022Quote: ... with 5 mL ATCC media 1034 (modified PYNFH medium) containing 10% (v/v) heat-inactivated Fetal Bovine Serum (FBS) (#F9665-100mL, Sigma-Aldrich), hereafter growth media ...
-
bioRxiv - Biochemistry 2022Quote: ... A 5% (v/v) of washed >30 kDa FBS fraction was premixed with a 1:3 serial dilution of CaCl2 (#C5080, Sigma-Aldrich) diluted in 1X PBS (concentration range from 0.5 to 0 mM ...
-
bioRxiv - Cell Biology 2023Quote: ... on ice in LCMS-grade acetonitrile/methanol/water (2:2:1 v/v) with 1nmol scyllo-inositol internal standard per sample (Sigma Aldrich). Sample debris was then removed via centrifugation and polar metabolite extracts were dried using a SpeedVac Vacuum Concentrator ...
-
bioRxiv - Developmental Biology 2023Quote: ... the hydrophobic modification of microfluidic channels was performed by flushing both types of devices with 1% (v/v) trichloro(1H,1H,2H,2H-perfluorooctyl)silane (Sigma-Aldrich) in HFE-7500 (3M ...
-
bioRxiv - Plant Biology 2023Quote: ... Cell pellets were washed once with HEPES lysis buffer (50 mM HEPES, 100 mM NaCl, 5% (v/v) glycerol) and treated with 300 U/µl r-Lysozyme (Sigma, USA) for 15 min at 23 °C ...
-
bioRxiv - Neuroscience 2023Quote: ... Non-specific antibody binding was blocked by applying a mixture of 10% v/v ChemiBLOCKER (Cat# 2170, Millipore, Burlington, MA, U.S.A.) and 5% v/v normal donkey serum in PBS for 12 hours at room temperature with gentle agitation ...