Labshake search
Citations for Millipore Sigma :
3601 - 3650 of 8516 citations for V Bottom Microplate 96 well since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2020Quote: Staining of plant root cells for loss of cell viability was carried out by staining freshly harvested roots from the control with Evans blue aquatic solution (0.25% v/v Evans blue, Sigma-Aldrich, Spain) for 10–15 min at room temperature followed by washing with CaCl2 solution (100 μm ...
-
bioRxiv - Plant Biology 2020Quote: ... 0.05% Triton X-100) supplemented with 1/500 (v/v) plant-specific protease inhibitor cocktail and phosphatase inhibitor cocktail 3 (Sigma-Aldrich). 20 μL (bed volume ...
-
bioRxiv - Systems Biology 2021Quote: ... 1.4 mL of a vitamin solution [105] and 1.4 mL of 20% (v/v) Antifoam emulsion C (Sigma, St. Louise, USA). During the fermentation 0.5 mL extra antifoam was added when necessary ...
-
bioRxiv - Microbiology 2020Quote: ... to obtain a final concentration of 80 % acetone (v/v) and pipetted on an Ultrafree-MC PTFE membrane 0.2 µm filter (Merck Millipore, Burlington, MA). The filter was incubated for 2 min at room temperature and was then centrifuged at 5000 x g for 1 min ...
-
bioRxiv - Biochemistry 2021Quote: ... were cultured in Roswell Park Memorial Institute-1640 media supplemented with 10% (v/v) heat-inactivated foetal bovine serum (Sigma-Aldrich) and maintained at 37°C with 5% CO2 ...
-
bioRxiv - Bioengineering 2021Quote: ... once with a 1% SDS-TRIS pH 11 solution (5% v/v of 20% sodium dodecyl sulfate #05030, from Sigma Aldrich and 2.4 % w/v TRIS base PBP151-500 from Fisher Scientific in reverse osmosis water ...
-
bioRxiv - Neuroscience 2020Quote: ... Axonal ChR2 expression was revealed by incubating slices for 1-2 h in Triton PBS containing 10% v/v NDS (D9663, Sigma, RRID:AB_2810235), then overnight at room temperature in Triton PBS containing 1% v/v NDS and Alexa Fluor 488-conjugated rabbit anti-GFP (1:500 ...
-
bioRxiv - Neuroscience 2021Quote: ... each sample was mixed vigorously with the same volume of PCI (phenol:chloroform:isoamyl alcohol = 25:24:1, v/v) (Millipore Sigma cat#516726).
-
bioRxiv - Cell Biology 2021Quote: ... diluted in water for 20 min at RT followed by washing with PBS and fixation with 0.5 % (v/v) glutaraldehyde (Sigma-Aldrich, USA) for 15 min on ice ...
-
bioRxiv - Genomics 2021Quote: PDOs were fixed for 20 min in 4% (v/v) Roti Histofix (Carl Roth) followed by embedding into MicroTissues 3D Petri Dish micromolds (Sigma Aldrich) using 2 % (w/v ...
-
bioRxiv - Cell Biology 2022Quote: For vinculin staining hMSCs are fixed with ice cooled mixture of 1:1 (v/v) (4% PFA: permeabilizing buffer (1% Triton-X-100-Sigma Aldrich) for one minute on ice ...
-
bioRxiv - Biochemistry 2022Quote: ... A pre-gel solution for the collagen hydrogel (final concentration: 4 mg/mL) was prepared by mixing the collagen solution with 10% (v/v) 10× PBS (Sigma-Aldrich) and 10% (v/v ...
-
bioRxiv - Biochemistry 2022Quote: ... cells were treated with 20 ng/mL BMP4 in a hepatic stellate cell basal medium composed of 40% (v/v) MCDB201 (Sigma-Aldrich), 57% (v/v ...
-
bioRxiv - Neuroscience 2022Quote: ... Fixed samples were washed in distilled water for 30 minutes before 21 days of decalcification in 14% (v/v) ethylenediaminetetraacetic acid (EDTA, Sigma, UK) solution (pH 8 ...
-
bioRxiv - Biophysics 2022Quote: ... Cover glasses were then activated by silanization with an ethanol solution containing 1% v/v (3-Aminopropyl) trimethoxysilane (Sigma Aldrich, 281778) for 15 minutes ...
-
bioRxiv - Biophysics 2022Quote: ... supplemented with 0.1 mg mL-1 Lysozyme (from a 100 mg mL-1 stock in 50 % [v/v] Glycerol, kept at -20 °C, Millipore Sigma), 50 Units of Benzonase per mL (Merck/Millipore Sigma ...
-
bioRxiv - Bioengineering 2022Quote: Cells were resuspended to a density of 5-10 million cells/mL in DMEM supplemented with 16-18% v/v OptiPrep (Sigma D1556) to maintain cells at neutral buoyancy and thus prevent sedimentation in the inlet (which would lead to uneven cell counts in the chambers)(38–40) ...
-
bioRxiv - Cell Biology 2022Quote: ... Isolated islets were allowed to recover overnight at 37°C in 5% CO2 in RPMI-1640 supplemented with 10% v/v fetal bovine serum (FBS) (F7524, Sigma-Aldrich) and 1% v/v Penicillin/Streptomycin (P/S ...
-
bioRxiv - Plant Biology 2021Quote: ... Lipids extracts were incubated 1 h at 50°C in 2 mL of methylamine solution (7 ml methylamine 33% (w/v) in EtOH combined with 3 mL of methylamine 40% (w/v) in water (Sigma Aldrich) in order to hydrolyze phospholipids ...
-
bioRxiv - Microbiology 2020Quote: ... Coverslips were washed 3 times with PBS and mounted on a microscope slide with parafilm gasket in 1% (v/v) β-mercaptoethanol (Sigma), 150 mM Tris ...
-
bioRxiv - Biophysics 2020Quote: ... both the support and cover glass were washed by immersion in a near-boiling 2% (v/v) Hellmanex III solution (Sigma-Aldrich) followed by sonication for 30 minutes ...
-
bioRxiv - Microbiology 2020Quote: ... the efficacy of the tested products was examined as an 80% solution in the presence of 10% interfering substance (5% (w/v) BSA Fraction V (Sigma Aldrich), 0.4% (w/v ...
-
bioRxiv - Cancer Biology 2021Quote: ... cells were re-suspended in fresh medium and viability and cell numbers were monitored with vital dye Trypan Blue 0.2% w/v (Trypan Blue 0.4% w/v, Sigma-Aldrich, St. Louis). The starting number of cells for the titration experiments on cells cultured under adherent conditions was set at 2 × 104 cells in 100μl of culture medium ...
-
bioRxiv - Microbiology 2021Quote: ... 1 mL of these extracts were centrifuged at 14,000 xg for 1 min at 4°C with 2.5 μl 2% (v/v) Tween 20 (Sigma-Aldrich, Darmstadt, Germany) to promote the aggregation into a pellet ...
-
bioRxiv - Biochemistry 2020Quote: ... The proteins were eluted with phosphate buffered saline containing 300 mM imidazole and 0.1% (v/v) protease inhibitor cocktail (Sigma, P-8849). The proteins were further purified using size-exclusion chromatography ...
-
bioRxiv - Pathology 2022Quote: ... at room temperature for 10 minutes and washed 3 times in PBS containing 0.1 % v/v Triton X-100 (Sigma-Aldrich X100), blocked for 30 min using 10 % v/v chicken serum (Gibco 16110082 ...
-
bioRxiv - Biochemistry 2019Quote: ... the membranes were incubated in TBST-5 % w/v milk (TBST supplemented with 5 % (w/v) skimmed milk powder) with anti-rabbit-HRP secondary antibody (A0545, Sigma Aldrich) (1:5,000 ...
-
bioRxiv - Plant Biology 2019Quote: ... 2004) containing 1% (w/v) sucrose and solidified with 1% (w/v) EDTA-washed Agar Type M (Sigma-Aldrich, Steinheim, Germany), followed by stratification in the dark at 4 °C for 2 d ...
-
bioRxiv - Cancer Biology 2019Quote: ... Colonies were fixed with 10% (v/v) glutaraldehyde for 30 minutes and stained with crystal violet (Sigma-Aldrich, St. Louis, MO) for 20 minutes ...
-
bioRxiv - Systems Biology 2019Quote: ... Samples were diluted to a 1 mg/mL protein concentration using 50 mM ammonium bicarbonate and incubated overnight with trypsin (1:200 v/v, Sigma #T6567). Digested samples were acidified and urea removed using a Sep-Pak C18 96-well plate (Waters #186002320) ...
-
bioRxiv - Genomics 2019Quote: ... The cells were resuspended in 500 μl of ice-cold lysis buffer (10 mM Tris-HCl, pH8.0, 10 mM NaCl and 0.2% (v/v) Igepal CA-630 (Sigma, 18896-100 ML)) containing 1x proteinase inhibitor complex (PIC ...
-
bioRxiv - Cancer Biology 2020Quote: ... Both mediums were supplemented with 1% penicillin/streptomycin and 10% (v/v) fetal calf serum (Sigma-Aldrich, St. Louis, MO, USA) and grown at 37°C ...
-
bioRxiv - Molecular Biology 2021Quote: ... The emulsions were then broken using 10 μL of 10% (v/v) solution of perfluoro-octanol (Sigma-Aldrich, catalog no. 370533), followed by gentle vortexing for 5 s and centrifugation for 1 min (Benchmark Scientific ...
-
bioRxiv - Molecular Biology 2021Quote: ... the hydrophobic modification of microfluidic channels was performed by flushing the device with 1% (v/v) trichloro(1H,1H,2H,2H-perfluorooctyl)-silane (Sigma-Aldrich) in HFE-7500 (3M ...
-
bioRxiv - Immunology 2020Quote: ... Stained cells were sorted on a BD Aria II or fixed in PBS/1% (v/v) formaldehyde/1 μM DAPI (Sigma-Aldrich) and analyzed by BD flow cytometers (Fortessa or LSR II) ...
-
bioRxiv - Biochemistry 2021Quote: ... A small-scale culture was grown in LB medium at 37 °C and was added at 1:10 V/V to 1L of a modified M9 medium containing MEM vitamin mix 1X (Sigma-Aldrich), 4 g of glucose ...
-
bioRxiv - Plant Biology 2020Quote: ... and placed in Petri dishes filled with 0.5% agarose containing 35 mM β-mercaptoethanol (v/v) and 1.5 mg/mL of 5-bromo-4-chloro-3-indolyl-β-D-galactoside (Sigma). For X-gal staining ...
-
bioRxiv - Cell Biology 2020Quote: ... Devices were bonded to PDMS-coated glass microscope slides using a 30 s oxygen plasma treatment (Femto, Deiner Electronic) followed by surface functionalisation using 1% (v/v) trichloro(1H,1H,2H,2H-perfluorooctyl)silane (Sigma Aldrich) in HFE-7500™ (3M™ Novec™) ...
-
bioRxiv - Immunology 2020Quote: ... Tissues were then washed with wash buffer and blocked for 1 hour at room temperature with 1x TBS IHC Wash Buffer with Tween 20 with 3% (v/v) normal donkey serum (Sigma-Aldrich), 0.1% (v/v ...
-
bioRxiv - Immunology 2020Quote: ... The first antibody cocktail was prepared in 1x TBS IHC Wash Buffer with Tween 20 with 3% (v/v) normal donkey serum (Sigma-Aldrich) and filtered through a 0.1 μm centrifugal filter (Millipore ...
-
bioRxiv - Biochemistry 2019Quote: 100% ethanol was added to the proteome digest to 40% v/v before addition of undecanal (EMD Millipore, cat. no. 8410150025) at an undecanal/peptide ratio of 20:1 w/w (50:1 for Arabidopsis seedling samples ...
-
bioRxiv - Cell Biology 2019Quote: ... The serum-free medium –SerM was prepared as described previously by replacing 10% v/v heat-inactivated human serum with oleic and palmitic acid (Sigma Aldrich) in a final concentration of 30 µM each ...
-
bioRxiv - Immunology 2019Quote: ... Heat-mediated antigen retrieval was performed with 10mM sodium citrate and non-specific staining reduced by incubation in 5% (v/v) goat serum (#G9023, Sigma Aldrich) for 30 mins at RT ...
-
bioRxiv - Microbiology 2019Quote: The extracted lipids were concentrated to dryness under nitrogen stream and then methylated by the presence of methanol and sulphuric acid (5% v/v) for 2 h at 85°C in the presence of 50 μg heptanoic acid (Sigma Aldrich) as internal standard ...
-
bioRxiv - Plant Biology 2019Quote: ... in the presence of 1 % v/v proteinase inhibitor mixture (Serva) and 0.5 % of each phosphatase inhibitor cocktails 2 & 3 (Sigma-Aldrich, Germany) in a Dounce Homogenizer ...
-
bioRxiv - Immunology 2021Quote: ... Control wells were incubated with equivalent volumes of corresponding cell culture medium and/or a solution of 10% (v/v) dimethyl sulfoxide (DMSO, Sigma-Aldrich) in DPBS ...
-
bioRxiv - Physiology 2021Quote: ... Samples were then immersed in 5% (v/v) H2O2 in methanol for up to 3 hours (Sigma-Aldrich Cat No: H1009) for bleaching ...
-
bioRxiv - Biochemistry 2020Quote: ... To form the bilayer the aperture was first pre-painted with 1 μL of 1% (v/v) hexadecane in pentane (Sigma Aldrich). One of the electrodes was used as ground (cis ...
-
bioRxiv - Neuroscience 2021Quote: ... inner ear was immersed in 25 % Quadrol (diluted with H2O to a final concentration of 25% v/v, 122262, Sigma-Aldrich) for 2 days with daily change and 5% ammonium solution (diluted with H2O to a final concentration of 5% v/v ...
-
bioRxiv - Neuroscience 2021Quote: ... for 2 days with daily change and 5% ammonium solution (diluted with H2O to a final concentration of 5% v/v, 105432, Sigma-Aldrich) for 6h to decolorize ...