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Citations for Millipore Sigma :
3651 - 3700 of 10000+ citations for IL 3 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... 4 μl/ml of human fibronectin (1 mg/ml; Sigma-Aldrich), and 10 μl of 1 N NaOH (Sigma-Aldrich ...
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Characterization of pyruvate dehydrogenase complex E1 alpha and beta subunits of Mycoplasma synoviaebioRxiv - Microbiology 2019Quote: ... Recombinant rMSPDHA and rMSPDHB were then expressed through 1.0 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) induction and purified using Ni-NTA His-Bind® Resin Kit (Novagen, San Diego, USA). The purified protein products were quantified by using BCA Protein Assay Kit (Thermo Scientific-Pierce ...
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bioRxiv - Plant Biology 2019Quote: ... and axr3-3 were expressed as 6X His-tag (N-terminal) fusion proteins in Escherichia coli strain Rosetta™ DE3 competent cells (Supplementary Table 4; Novagen, product code: 70954). These proteins were expressed in minimal media with 13C D-glucose and 15N ammonium chloride ...
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bioRxiv - Biochemistry 2021Quote: ... His-tagged CLIP-170 fragments and mutants were expressed in BL21 (DE3) and purified by the standard His-tagged purification protocol from Novagen (69670-5, Sigma-Aldrich) with the following modifications ...
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bioRxiv - Molecular Biology 2019Quote: ... and zinc binding proteins were eluted according to the supplied protocol using HIS-Select wash and elution buffers purchased from Sigma-Aldrich (Saint Louis, MO).
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bioRxiv - Genomics 2022Quote: ... or T-175 flasks (Hi-C, CUT&RUN) at a density of 1 × 105 cells/mL and treated with 25 nM PMA (Sigma-Aldrich, cat # P1585-1MG). After 24h ...
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bioRxiv - Biochemistry 2023Quote: Thermal stability of BSA and HSA variants (His-tagged form) and of commercial wild type HSA and BSA (Sigma-Aldrich A1653 and A7638, respectively) was measured by nanoscale differential scanning fluorimetry (nanoDSF) ...
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bioRxiv - Neuroscience 2022Quote: ... and triethyl amine were made in MilliQ ultrapure water (18 megaohm resistance) and 1-octen-3-ol was diluted in paraffin oil (EMD Millipore #PX0045-3). Aliquots of 10 µL of each compound and dilution was pipetted onto a piece of filter paper (5 × 20 mm ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were then washed 3 times with PBS and incubated with an anti-active Caspase-3 antibody (EMD Millipore, Billerica, MA, 1:200) for 2h at room temperature ...
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bioRxiv - Bioengineering 2021Quote: ... they were anaesthetized in 3-amino benzoic acid ethyl ester (Tricaine/ethyl 3-aminobenzoate; Sigma Aldrich; 168 μg·ml−1 in Tris pH 7) and embedded in 1% low melting point agarose (UltraPure Agarose ...
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bioRxiv - Neuroscience 2020Quote: ... N- (Piperidin-1-yl)-5- (4-iodophenyl)-1- (2,4-dichlorophenyl)-4-methyl-1H-pyrazole-3-carboxamide (AM251, 3 mg/kg; Sigma Aldrich, St. Louis, MO), or vehicle (VEH ...
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bioRxiv - Genetics 2021Quote: ... Mouse neural progenitor cells were routinely passaged 1:2-1:4 every 3-5 days using Accutase and maintained in NS expansion medium on laminin-coated plates (Sigma, 3 μg/ml). Both mESCs and mNPCs were incubated at 37 °C and 5% CO2 ...
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bioRxiv - Genetics 2020Quote: ... containing either tunicamycin only (Enzo Life-Sciences; 2-3 μg/mL) or tunicamycin and auxin (IAA, indole-3-acetic acid, Sigma-Aldrich; 1 mM), and seeded with OP50-1 bacteria ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue was mounted on charged slides and dried prior to ethanol washes (70%, 80%, 95%, 100% Ethanol, 3 minutes each) and washed in a histological clearing agent (Xylene substitute; Sigma Aldrich; 3 minutes), and then cover slipped with DPX mounting agent (Sigma Aldrich).
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bioRxiv - Neuroscience 2023Quote: ... The excess fluorescent probe was removed through molecular weight and the buffer was exchanged with PBS (pH 7.4) using 3 kDa centrifugal filter units (UFC500396, Amicon® Ultra – 0.5mL, 3 kDa, Sigma-Aldrich®, Dublin, Ireland). Absorbance was measured at 555 and 280 nm for all tagged samples and calculations were performed according to manufacturer’s instructions using an arbitrary extinction coefficient of 100,000 and molecular mass of 100,000 SI unit ...
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bioRxiv - Biophysics 2022Quote: ... SPR2 DNA encoding residues 649-864 was generated using the polymerase chain reaction method (primers: 5’-GGCAGGACCCATATGGGCAGGAGAGGGTGGGATAATAAAGC-3’ and 5’-GCCGAGCCTGAATTCTTACTTGTCGAACTGTTGGAGATCGATTTC-3’) and individually sub-cloned into pET28 (Millipore Sigma, Burlington, MA) using engineered NdeI and EcoRI restriction endonuclease sites ...
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bioRxiv - Microbiology 2024Quote: ... The chromatic mutants were mixed in different ratios (1:1, 1:3, 3:1) and the mixtures were fixed using 4% paraformaldehyde (Sigma−Aldrich/Merck, Germany) for 4 h at 4°C.
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bioRxiv - Immunology 2021Quote: ... Cells were cultured in a 24-well plate (500’000 cells/well) in complete IMDM supplemented with 6 ng/ml IL-6 (Sigma), 6 ng/ml IL-3 (eBioscience ...
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bioRxiv - Neuroscience 2021Quote: Plasma soluble TREM2 (sTREM2) levels were measured using a Luminex 200 3.1 xPONENT System (EMD Millipore, Chicago, IL, USA) and a custom detection method designed to capture the soluble portion of TREM2 protein as previously described (24) ...
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bioRxiv - Immunology 2019Quote: ... IL-17 and CCL17 were determined with ELISA assays according to the manufacturer’s protocols (Sigma, St. Louis, MO, USA).
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bioRxiv - Cancer Biology 2024Quote: ... Benita Katzenellenbogen (University of Illinois at Urbana-Champaign, IL) and were maintained in minimal essential medium (MEM; Sigma M1018) supplemented with 5% calf serum (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... 20 U/mL IL-2 (AIDS Reagent Program, Germantown, MD) and 1% penicillin/streptomycin (Sigma- Aldrich, St. Louis, MO), Cells were activated for 3 days with 1 μg/mL/million cells of purified NA/LE mouse anti-human CD3 and CD28 (BD Biosciences ...
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bioRxiv - Cell Biology 2020Quote: ... or cellulose solution in N-hydroxysuccinimide (NHS; Aldrich; 100 mM) and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC, Sigma; 100 mM, 90 min, RT)44 ...
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bioRxiv - Microbiology 2019Quote: ... 5α-androstan-17β-ol-3-one (=17β-hydroxyandrostan-3-one) and 3aα-H-4α(3’-propanoate)-7aβ-methylhexahydro-1,5-indanedione (HIP) were obtained from Sigma-Aldrich (St. Louis, MO, USA). 5α-androstan-3β,17β-diol (=3β,17β-dihydroxyandrostane ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... peptides (<3 kDa) were separated from high molecular size proteins by a 3 kDa cutoff ultrafiltration column (Amicon®, Merck Millipore Ltd., Cork, Ireland). The crude peptides (<3 kDa ...
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bioRxiv - Immunology 2021Quote: The livers of freshly-sacrificed mice were perfused retrogradely via the IVC(3) with 3 ml of PBS and then 10 ml of 2% paraformaldehyde (Sigma, catalogue# 30525-89-4) in PBS ...
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bioRxiv - Immunology 2020Quote: ... injection of 106 hemocytes from late third instar larvae of the lethal(3)malignant blood neoplasm [l(3)mbn1] mutant larvae in Drosophila Ringer’s solution (Sigma-Aldrich, St. Louis, MI, USA). Booster injections were given 4 ...
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bioRxiv - Biophysics 2023Quote: ... The surface of the coverslips were then coated with a solution of 3% (v/v) (3-Aminopropyl) triethoxysilane (APTES, Sigma-Aldrich, St. Louis, MO) in ethanol for 20 min at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: When the tumor sizes reached to 80 mm3 animals were treated with a PFKFB3 inhibitor 3-(3-pyridinyl)-1-(4-pyridinyl)-2-propen-1-one) (3PO) (Sigma-Aldrich, Merck, Overijse, Belgium). The animals received intraperitoneal (i.p. ...
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bioRxiv - Biophysics 2023Quote: 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) dry powders were purchased from Sigma Aldrich (St. Louis, MO, USA). Different amounts of DOPC and DOTAP were used to tune the surface charge of the employed liposomes ...
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bioRxiv - Cell Biology 2023Quote: ... strains containing a degron tag were arrested at 30 °C for 3 hours (total) and then treated with 250 μM 3-indole acetic acid (Sigma-Alrich, St. Louis, MO) for 30 minutes before harvesting ...
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bioRxiv - Molecular Biology 2023Quote: ... The expression of 3 × FLAG-tagged MpRBOHB proteins was confirmed by western blot analysis using an anti-3 × FLAG antibody (Sigma-Aldrich, Cat. No. F1804).
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bioRxiv - Biophysics 2023Quote: ... the quench buffer also included 3 μL of 0.8% GDN and 3 μL of 300 mg/ml aqueous suspension of ZrO2-coated silica (Sigma-Aldrich, reference no. 55261-U). The resulting mixture was incubated on ice for 1 min to remove lipids and solubilize prestin in GDN before being filtered through a cellulose acetate spin cup (Thermo Pierce ...
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bioRxiv - Plant Biology 2020Quote: ... 150 µM 3′,5′-Dimethoxy-4′-hydroxyacetophenone (acetosyringone; SIGMA, USA) at a 23.7×108 cfu/ml (OD600=0.75 ...
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bioRxiv - Neuroscience 2021Quote: ... for 3 h followed by ATP (2 mM, Sigma Aldrich, A6419-5g ...
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bioRxiv - Cell Biology 2019Quote: ... rabbit polyclonal anti-PCSK1 (PC1/3) (1:1000, Sigma, #SAB1100415), rabbit polyclonal anti-TSSC1/EIPR1 (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 200 μM 3-isobutyl-1-methylxanthine (IBMX; Sigma), and their cumulus cells were removed by gentle pipetting ...
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bioRxiv - Cell Biology 2020Quote: ... 250 μM 3-isobutyl-1-methylxanthine (IBMX, Sigma-Aldrich # I5879), 1 μM rosiglitazone (Cayman Chemical # 71742) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were blocked and simultaneously permeabilized with 3% BSA (SIGMA) and 0.2% Triton X-100 (SIGMA ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were subsequently blocked and permeabilized with 3% BSA (SIGMA) and 0.2 w/v % Saponin (Millipore ...
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bioRxiv - Cell Biology 2020Quote: ... or 3 µg/cm2 poly-L-lysine (Sigma-Aldrich, P6407) overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... 1x Phosphatase inhibitor cocktail 2 and 3 from Sigma-Aldrich) ...
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bioRxiv - Immunology 2021Quote: ... and then blocked with 3% (w/v) BSA (Sigma-Aldrich) and 0.05% (v/v ...
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bioRxiv - Molecular Biology 2020Quote: ... 3-15 µM MG132 (Z-Leu-Leu-Leu-al, Sigma) was added to the media for the indicated hours prior to cell lysis/fixation ...
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bioRxiv - Developmental Biology 2021Quote: ... anti-Caspase-3 antibody (Sigma USA, Dubey and Tapadia, 2017) was used at a dilution of 1:100 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and 30 μL D2O with 3%TPS (Sigma Aldrich, 450510) and transferred in a standard 5 mm NMR tube (Wilmad-LabGlass ...
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bioRxiv - Neuroscience 2022Quote: ... propyl acetate and 3-methyl-2-buten-1-ol (Sigma). Odorants were selected based on previous work using this task (Gu & Li ...
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bioRxiv - Biophysics 2022Quote: ... 250 μM 3-isobutyl-1-me-thylxantine IBMX (Sigma-Aldrich), 100nM cortisol (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... 1X Phosphatase inhibitor cocktails 2 and 3 (Sigma P5726, P0044), and 1X Protease inhibitor tablets (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... and dispase-II (3 mg/mL, Cat# D4693, Sigma-Aldrich) for 60 min at 37 °C ...