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Citations for Millipore Sigma :
3601 - 3650 of 3948 citations for IL 23R Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: Mice were injected subcutaneously under anaesthesia into the right flank with 5 × 106 human bladder cancer cells (RT112) in RPMI medium (Sigma Aldrich) with phenol red-free Matrigel (BD Biosciences ...
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bioRxiv - Neuroscience 2020Quote: Mating between pairs of adult Xenopus laevis was induced regularly by injections of human chorionic gonadotropin (HCG, 1000 U/ml, Sigma, UK) into the dorsal lymph sacs ...
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bioRxiv - Molecular Biology 2020Quote: ... a single fluorescent cell was sorted per well of a fibronectin-coated (10 μg/mL Human Plasma Fibronectin Purified Protein (Sigma Aldrich) in 1x PBS ...
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bioRxiv - Neuroscience 2021Quote: ... Cell lines stably expressing shRNA to human FBXO7 were generated as described previously (20) and selected with 2 μg/mL puromycin (Sigma-Aldrich).
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bioRxiv - Microbiology 2020Quote: ... Chips were then washed thoroughly with PBS before incubating with an ECM solution of 150 μg/mL bovine collagen type I (AteloCell, Japan) and 30 μg/mL fibronectin from human plasma (Sigma-Aldrich) in PBS buffered with 15 mM HEPES solution (Gibco ...
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bioRxiv - Biochemistry 2021Quote: The competitive inhibition of human fumarase activity in the presence of 2-amino-3-phosphonopropionic acid (AP-3, Sigma-Aldrich A4910) was fluorometrically assessed using a coupled enzyme assay ...
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bioRxiv - Bioengineering 2021Quote: ... were coated with 100 μl (5 μg/ml) of purified S1 or S1-RBD or commercial human serum albumin (HSA; Sigma-Aldrich) and incubated overnight at 4°C ...
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bioRxiv - Bioengineering 2020Quote: ... 16 hours post transfection gene expression from the human CMV immediate-early gene enhancer/promoter was induced with 10 mM sodium butyrate (Sigma-Aldrich) for 6 hours before fresh media was added to the cells ...
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bioRxiv - Cancer Biology 2020Quote: Biotin labelled and unlabeled RNA oligonucleotides probes corresponding to the human cMYC ARE (1-6) and two random probes (R1, R2) were synthesized as indicated in Table S5 by Sigma-Aldrich. REMSA was performed with a LightShift chemiluminescent RNA EMSA Kit (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: Adipogenic differentiation of ASC was induced during 14 days with human adipocyte differentiation medium [DMEM/F12 50:50 and the following reagents from Sigma-Aldrich Inc. ...
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bioRxiv - Developmental Biology 2022Quote: ... A pair of adults were placed in a 10-gallon tank filled with 10% Holtfreter solution (Holtfreter, 1931) and injected with 10 international units (IU) of human chorionic gonadotropin (HCG; Sigma CG10) into the dorsal lymph sac ...
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bioRxiv - Genomics 2022Quote: ... Mouse gRNA constructs and pCMV-sadCas9-KRAB (kind gift from Dr. Alejandro Lomniczi OHSU) were co-transfected into human preadipocytes X-tremeGene (Sigma, 6366236001) while human gRNA plasmids and pCMV-sadCas9-KRAB were co-transfected into mouse 3T3-L1 preadipocytes ...
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bioRxiv - Molecular Biology 2022Quote: ... Secretion of the following cytokines was quantified with the MILLIPLEX® Human CD8+ T Cell Magnetic Bead Panel Premixed 17 Plex - Immunology Multiplex Assay kit (EMD-Millipore): IL-6 ...
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bioRxiv - Cancer Biology 2022Quote: ... 2-3 mm3 tumor pieces were cut and placed in a 24-well plate containing RPMI medium supplemented with 10% human AB serum (Sigma-Aldrich), 6000 IU/ml human recombinant IL2 (Peprotech) ...
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bioRxiv - Systems Biology 2020Quote: ... one well containing peripheral blood monocyte- or human pluripotent stem cell-derived macrophages were stimulated with 10ng/ml Lipopolysaccharide (LPS) (Sigma-Aldrich; Salmonella enterica serotype minnesota ...
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bioRxiv - Immunology 2019Quote: Multiplex bead assays for plasma samples were performed in duplicate for TNF from the MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel (Millipore Sigma). Co-culture supernatants were assayed in duplicate for IgG1 production using the Legendplex Human Immunoglobulin Isotyping Panel (Biolegend ...
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bioRxiv - Microbiology 2019Quote: ... we performed growth curves in YPD liquid medium containing different concentrations of Gal-3 (Gal-3 human recombinant, expressed in E. coli, Sigma-Aldrich) in a 96-well plate (Costar ...
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bioRxiv - Microbiology 2019Quote: ... 1.8 μg of pRSV-Rev and 1.5 μg of plasmid containing pAIP-NSP2-mCherry or pAIP-NSP5 and the human immunodeficiency virus long terminal repeats were co-transfected with Lipofectamine 3000 (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... RPMI-1640 medium used to culture the MCF7 cells was additionally supplemented with 0.01 mg/ml human recombinant insulin (Sigma Aldrich, #I3536).
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bioRxiv - Cancer Biology 2019Quote: ... Human ovarian cancer cell line OV90 (ATCC CRL-11732) was cultured in 1:1 volume of MCDB 105 medium (Sigma-Aldrich) and Medium 199 (Gibco ...
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bioRxiv - Cancer Biology 2020Quote: ... All other cell lines were grown in Dulbecco`s modified Eagle medium (DMEM) (Fisher, UK) supplemented with 2% human serum (Sigma, UK) and maintained in an humidified sterile incubator with 5% CO2 at 37°C.
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bioRxiv - Molecular Biology 2020Quote: The 3×Flag-CPEB3 plasmid was generated by subcloning the PCR-amplified human CPEB3 cDNA into the p3×FLAG-CMV 10 vector (Sigma-Aldrich). The human CPEB3 cDNA was synthesized using the indicated primers ...
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bioRxiv - Cell Biology 2021Quote: ... 250 µl of maintenance medium (50% Wnt3a-conditioned medium (ATCC#CRL-2647, Manassas, VA, USA) and 50% of 2× basal medium supplemented with recombinant human EGF (Sigma-Aldrich), recombinant human noggin (R&D Systems ...
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bioRxiv - Developmental Biology 2020Quote: ... Females were superovulated at 6-8 weeks with 0.1 ml CARD HyperOva (Cosmo Bio Co., Cat. No. KYD-010-EX) and 5 IU hCG (Human chorionic gonadotrophin, Sigma-Aldrich) at intervals of 48 hours ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... HNECs were then seeded on 12 mm transwell inserts with 0.4 μm pores (Costar 3460) coated with human placental collagen (Sigma C7521-10MG) at a density of 203,000-333,000 cells per well and maintained in PneumaCult -Ex Plus Medium ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed 6 times with 225 µL ELISA wash buffer and 1:5000 goat anti-human IgG HRP (Millipore Sigma) or anti-human IgG isotype specific HRP (Southern Biotech ...
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bioRxiv - Cell Biology 2019Quote: ... The serum-free medium –SerM was prepared as described previously by replacing 10% v/v heat-inactivated human serum with oleic and palmitic acid (Sigma Aldrich) in a final concentration of 30 µM each ...
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bioRxiv - Immunology 2021Quote: ... The cells were then treated with SplB (5 μg/mL) or increasing concentrations of human plasma thrombin (PAR1 and PAR4 agonist, Calbiochem-EMD Millipore) or porcine trypsin (PAR2 agonist ...
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bioRxiv - Cell Biology 2021Quote: Human leukaemia cells HL-60 and breast cancer cell line MCF-7 were cultured in RPMI and DMEM media (Sigma-Aldrich), respectively ...
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bioRxiv - Microbiology 2021Quote: ... falciparum parasites were maintained in vitro in human O+ erythrocytes at 4% haematocrit in RPMI 1640 medium supplemented with 25mM HEPES (Sigma-Aldrich), 0.25% sodium bicarbonate ...
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bioRxiv - Bioengineering 2021Quote: ... or type I collagen microchannels coated overnight with 50 μg mL−1 human placental collagen IV (Cat. no. C5533, Sigma-Aldrich) and 25 μg mL−1 fibronectin from human plasma (Cat ...
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bioRxiv - Developmental Biology 2020Quote: ... 4-weeks old female mice were injected intraperitoneally with 5IU Pregnant Mare Serum Gonadotropin (PMSG, Prospec) followed by 5 IU human Chorionic Gonadotropin (hCG, Sigma-Aldrich) 46-48 hours later ...
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bioRxiv - Cell Biology 2020Quote: ... DOPA coated PA-gels were washed 2x with sterile PBS and ECM functionalized with 5 μg/mL human fibronectin (Millipore, FC010) in sterile PBS 1 h at 37 °C to generate an expected fibronectin coating density of 6 μM/cm2.
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bioRxiv - Cancer Biology 2021Quote: Selective depletion of the myeloid cell compartment in human neocortical slices was performed by supplementing the growth medium with 11 μmol of Clodronate (Sigma, D4434) for 72h at 37ºC ...
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bioRxiv - Biochemistry 2021Quote: ... Rabbit anti-SNAP-25 polyclonal antibody raised against a synthetic peptide corresponding to the N-terminus of human SNAP-25 (Sigma S9684), was used as a positive control to detect both cleaved and intact SNAP-25 ...
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bioRxiv - Biochemistry 2021Quote: ... was cultivated in fresh human erythrocytes (either A⁺ or O+) in RPMI-1640 culture medium supplemented with 25 mM HEPES (pH 7.5, Sigma Aldrich, USA), 0.2 mM hypoxanthine (Sigma Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... asexual blood stage cultures were maintained at 0.75-5% parasitaemia and 4% haematocrit using O+ or A+ human erythrocytes (NHS National Blood Service) supplemented with 30 units/ml heparin (Sigma-Aldrich). Culture medium was prepared from RPMI-1640 with 25mM HEPES (Life Technologies) ...
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bioRxiv - Developmental Biology 2021Quote: ... 4-weeks old female mice were injected intraperitoneally with 5IU Pregnant Mare Serum Gonadotropin (PMSG, Prospec) followed by 5 IU human Chorionic Gonadotropin (hCG, Sigma-Aldrich) 46-48 hours later ...
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bioRxiv - Cell Biology 2020Quote: ... containing a cDNA sequence of WT human CAV1 codon optimized for E.coli expression and cloned into a pET20 vector (Novagen No. 69739) using Nde I and Xho I restriction sites ...
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bioRxiv - Genomics 2020Quote: ... of pregnant mare’s serum gonadotrophin (PMSG) (USBiological Life Sciences G8575A) and 5 IU of human chorionic gonadotrophin (hCG) (Sigma-Aldrich C1063) 46-48 h later ...
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bioRxiv - Cancer Biology 2021Quote: Human embryonic kidney (HEK) 293T cells and human non-small-cell lung cancer (NSCLC) A549 cells were cultured in DMEM (Sigma-Aldrich) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2020Quote: Human bronchial epithelial cells (BEAS-2B) were obtained (ATCC, Manassas, VA, USA) and maintained in HAM’s F12 (Sigma-Aldrich, MO, USA) with 10% fetal bovine serum ...
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bioRxiv - Immunology 2021Quote: ... SARS2-16 (hybridoma supernatant diluted 1:6,000 to a final concentration of ∼20 ng/mL) and then HRP-conjugated goat anti-human IgG (Sigma-Aldrich, A6029) in PBS supplemented with 0.1% (w/v ...
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bioRxiv - Immunology 2021Quote: ... Then cells were stained with anti-mouse IgG Taxes red-conjugated antibody and anti-human IgG FITC-conjugated antibody (Sigma, USA) for another 30 min then analyzed by FACSAria II (BD ...
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bioRxiv - Immunology 2020Quote: ... The THP1-Dual and THP1-Dual KO (IRF3/TBK1/MyD) cells (human lung monocytes) were cultured in RPMI 1640 (Sigma-Aldrich) medium supplemented with 10% heat-inactivated FBS ...
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bioRxiv - Microbiology 2021Quote: ... and then seeded at a 1:4 dome-to-well ratio in a 96-well plate coated in 100 µL of 34 µg/mL human collagen IV (Sigma C5533). Cells were fed every two days and were used for experiments after they were fully confluent (4-5 days) ...
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bioRxiv - Microbiology 2021Quote: ... Plates were washed and bound human ACE2 was detected using HRP-conjugated anti-Flag antibody (Sigma-Aldrich, A8592, 1:5,000 dilution) and TMB substrate ...
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bioRxiv - Bioengineering 2021Quote: Day-21 TSC2+/+ organoids were incubated in Advanced RPMI + 1X L-GlutaMAX with 50ng/ml human IL1-β (Sigma-Aldrich # H6291) for 96hs ...
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bioRxiv - Neuroscience 2022Quote: ... were transfected into mouse hypothalamus Pomc neuronal cells (mHypoA-POMC/GFP-1 from Sanbio #CLU500) or human astrocytes (CCF-STTG, ATCC) grown in 96-well plates of 132 using X-tremeGene (Sigma, 6366244001) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... Females were super-ovulated at 6–8 weeks with 0.1 ml CARD HyperOva (Cosmo Bio Co., Cat. No. KYD-010-EX) and 5 IU hCG (Human chorionic gonadotrophin, Sigma-Aldrich) at intervals of 48 hours ...