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Citations for Millipore Sigma :
3601 - 3650 of 3691 citations for CD40 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Electroporated cells were cultured in erythroid differentiation medium (EDM) consisting of IMDM (GibcoTM, 12440061) supplemented with 330 µg/ml of Holo-Human Transferrin (Sigma-Aldrich, T0665-1G), 10 µg/ml of recombinant human insulin (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... Two different Mission lentivirus-based plasmids of shRNAs (clone numbers TRCN0000123050 and TRCN0000436778) against human ZBP1 and the shcontrol vector TRC2 pLKO.5-puro nonmammalian shRNA (SHC202) were obtained from Sigma-Aldrich (Burlington, MA). 293T cells were cotransfected with the shRNA and packaging plasmids psPAX2 and pMD2 using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... and HOS-ACE2/TMPRSS2 cells (HOS cells stably expressing human ACE2 and TMPRSS2)36,37 were maintained in DMEM (high glucose) (Sigma-Aldrich, Cat# 6429-500ML) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: ... East Brunswick, NJ, USA), human recombinant Stem Cell Factor (100ng/mL, supernatant SCF producing cell line) and dexamethasone (1μM; Sigma, St. Louis, MO, USA).5 EBL cultures were kept at a density of 0.7-1.5 million/mL by dilution with fresh medium ...
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bioRxiv - Microbiology 2022Quote: ... and HOS-ACE2/TMPRSS2 cells (HOS cells stably expressing human ACE2 and TMPRSS2)32,33 were maintained in DMEM (high glucose) (Sigma-Aldrich, Cat# 6429-500ML) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2023Quote: ... Cells that received Interferon-β pre-treatment were stimulated for 18 hours with 100 units of recombinant human Interferon-β protein (Millipore, IF014) prior to infection.
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bioRxiv - Immunology 2023Quote: ... human islets were dispersed using dissociation solution (1X TrypLE™ Express solution - Thermo Fisher Scientific, with 40 µg/mL DNase I - Sigma-Aldrich) through gentle pipetting for 10 minutes at 37°C ...
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Microhomology-Mediated Circular DNA Formation from Oligonucleosomal Fragments During SpermatogenesisbioRxiv - Genomics 2023Quote: ... The cauda epididymis was quickly cut into pieces and incubated in 1 ml pre-warmed human tubal fluid (HTF) (Millipore, MR-070-D) for 15 min at 37°C ...
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bioRxiv - Immunology 2023Quote: ... diluted in FACS-blocking buffer (mixture of 0.66% human/rabbit/mouse serum, Sigma-Aldrich, and 1% Bovine Serum Albumin, Sigma-Aldrich in PBS) for 30 minutes at 4°C ...
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bioRxiv - Genetics 2023Quote: ... The lentivirus enriched-medium was immediately used to transduce the human fibroblasts growing on a T25 flask in the presence of 8µg/ml polybrene (Sigma-Aldrich, San Luis, MO). Infected fibroblasts were maintained in culture until having enough cells for cell sorting ...
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bioRxiv - Neuroscience 2023Quote: ... and immediately incubated at 4°C in the primary antibody against the N-terminus of human Fos (overnight, 1:2000; Rabbit polyclonal, ABE457, Millipore; RRID: AB_2631318 (56) (Exp ...
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bioRxiv - Immunology 2023Quote: Concentrations of cytokines in supernatants of T cell cultures were assessed with the MILLIPLEX MAP Human Th17 magnetic bead panel kit (Merck Millipore, Burlington, MA, USA) and the Luminex® 200™ system according to the guidelines of the manufacturer ...
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bioRxiv - Immunology 2023Quote: ... ELISA signal in each elution sample was checked using 1:5000 diluted goat anti-human IgG (Fab specific) HRP-conjugated secondary antibodies (Sigma-Aldrich, A0293-1ML). Elution fractions showing an ELISA signal were pooled and concentrated under vacuum to a volume of ∼1 μL ...
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bioRxiv - Molecular Biology 2023Quote: ... and a subsequent 6-day differentiation step in serum-free medium containing 50 ng/ml human brain derived neurotrophic factor (BDNF) (Sigma-Aldrich, Cat# B3795). Cells were seeded at an initial density of 2 × 104 cells/cm2 in 24-well plate coated with Type I collagen (Corning ...
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bioRxiv - Cancer Biology 2023Quote: Mouse (5 x 105 cells) and human (2 x 105 cells) keratinocytes were seeded onto 12 mm diameter inserts (Millipore, Catalog No. PIHP01250) and cultured in CnT-Prime medium ...
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bioRxiv - Developmental Biology 2023Quote: ... The cell lysate was generated as a Western blot positive control from the human endometrial epithelium Ishikawa cell line (Sigma-Aldrich, Madrid, Spain), as in a previous study (Vilella et al. ...
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bioRxiv - Bioengineering 2024Quote: IDUA enzyme expression was measured fluorometrically in a 96-well plate using a Human IDUA ELISA kit (Sigma-Aldrich, St. Louis, MO, USA) according to the manufacturer’s sandwich assay protocol ...
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bioRxiv - Synthetic Biology 2023Quote: ... The CAR T cell product was cultivated in RPMI+10%FBS+1%PS at 37°C with 5% CO2 for experiments and cryopreserved as 10×106 cells/mL in 1 mL 90% heat-inactivated Human AB Serum (Sigma-Aldrich, Cat.#H4522) +10% DMSO in liquid nitrogen ...
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bioRxiv - Immunology 2024Quote: ... Total transferrin was kept constant at 1.2 mg/mL by adjusting human apotransferrin (unbound transferrin) concentrations (R&D systems, 3188-AT-001G/Sigma Aldrich, T1147) accordingly ...
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bioRxiv - Neuroscience 2024Quote: ... Both mouse and human brain sections were labeled for TH (mouse monoclonal, clone LNC1, 1:2000, Catalog No. MAB318, EMD Millipore, Burlington, MA) and VGLUT2 (rabbit polyclonal ...
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bioRxiv - Bioengineering 2024Quote: ... human chorionic gonadotropin (hCG, for Jurkat: high 1000 IU/mL and low 200 IU/mL; PBMC: 500 IU/mL, Sigma catalog #CG5-1VL). Media controls were used to determine if media components alone affect protein secretion and expression ...
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bioRxiv - Immunology 2021Quote: ... and Tumor necrosis factor-alpha (TNF-α)) were measured with a Milliplex MAP High-Sensitivity Human Cytokine Panel (HSCYTMAG-60SK-13, Millipore Corp., Billerica, MA) on a Luminex MagPix (Millipore Corp. ...
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bioRxiv - Molecular Biology 2020Quote: Cells were washed three times with saline at 200 × g for 10 min at RT to remove residual Percoll and suspended in RPMI 1640 medium (Cellgro, Manassas, VA) supplemented with 5% human serum (Sigma-Aldrich, St. Louis, MO), unless otherwise stated ...
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bioRxiv - Genomics 2019Quote: ... we measured 41 different cytokines and chemokines using the Milliplex MAP Human Cytokine/Chemokine Magnetic Bead Panel (Millipore kit no. HCVD3-67CKHCYTOMAG-60K, Millipore Corp, St. Charles, MO). According to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... and lymph node from BALB/cJ mice and humans were harvested and either fixed for 48 h at RT in 10% neutral buffered formalin (Sigma-Aldrich, St. Louis, MO) or zinc-fixation buffer (pH 6.5 - 7) ...
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bioRxiv - Neuroscience 2021Quote: ... mouse and human brain slices of 20 μm thickness were subjected to antigen retrieval in 10 mM citrate (Sigma-Aldrich, St. Louis, MO, USA) + 0.5% Tween-20 (Merck ...
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bioRxiv - Biophysics 2020Quote: ... Fibronectin coating was performed by covering the glass slides in a solution of DPBS with 10 μg/mL human fibronectin (EMD Millipore, Burlington, MA, USA) for at least 2 hours ...
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bioRxiv - Cell Biology 2021Quote: Labeling of septins on Western blots (WB) and/or on immunocytochemistry coverslip (ICC) was achieved with the following antibodies: rabbit polyclonals against human Sept2 (Sigma-Aldrich, Cat#HPA018481, WB), against human Sept9 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... Human chorionic gonadotropin (hCG) as an effective Lh (Luteinizing hormone) mimicking hormone used in this study was purchased from Sigma-Aldrich (Oakville, ON, Canada). There is evidence that hCG transactivate Lhcgr (Luteinizing hormone/choriogonadotropin receptor ...
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bioRxiv - Cancer Biology 2022Quote: ... Both these constructs were individually transfected in Ishikawa cells (human endometrial epithelial cells) using Xtreme Gene HP transfection reagent (Sigma Aldrich; Missouri, United States) as per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: Cytokines in BAL fluid samples were measured (pg/ml) in singlicate by Luminex using a Millipore human cytokine multiplex kits (EMD Millipore Corporation, Billerica, MA) according to manufactures instructions ...
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bioRxiv - Immunology 2021Quote: Cytokine and chemokine assays were performed by the Forsyth Multiplex Core (Cambridge, MA, USA) using the Human Cytokine/Chemokine Magnetic Bead Panel (Milliplex, Millipore Sigma, Burlington, MA, USA) and Bio-Plex®200 plate reader following manufacturers’ specifications ...
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bioRxiv - Microbiology 2022Quote: ... African green monkey kidney Vero’76 and human lung adenocarcinoma Calu-3 cells were maintained in DMEM (Sigma-Aldrich, D5796; St. Louis, MO, USA) supplemented with 10% fetal bovine serum (ThermoFisher ...
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bioRxiv - Microbiology 2022Quote: Levels of cytokines/chemokines in macaque plasma were measured using the Milliplex MAP Non-human Primate Cytokine Panel in combination with a Luminex 200 (Millipore Corp., Billerica, MA, USA) according to the manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2020Quote: HeLa cells were purchased from the Human Science Research Resources Bank and maintained in DMEM (Nacalai tesque, Kyoto, Japan) supplemented with 10% fetal bovine serum (Sigma-Aldrich, St. Louis, MO). BVRA-KO HeLa cells (HeLa/BVRA KO ...
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bioRxiv - Pathology 2019Quote: ... and TGF-β1 in the samples was measured using magnetic bead-based multiple immunoassays (MILLIPLEX® MAP kit, Human High Sensitivity T Cell Magnetic Bead Panel: EMD Millipore Corporation, Germany). Each assay included two calibration curves for each of the proteins to be measured (calibration ranges ...
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bioRxiv - Microbiology 2021Quote: ... Calu-3 cells (a human lung epithelial cell line; ATCC HTB-55) were maintained in Minimum essential medium Eagle (Sigma-Aldrich, cat# M4655-500ML) containing 10% FCS and 1% PS ...
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bioRxiv - Neuroscience 2020Quote: ... anti-amyloid precursor protein (APP; mouse anti-human monoclonal antibody clone 22c11, dilution 1:10, epitope retrieval time 10 min, MAB348; EMD Millipore, Burlington, MA, USA); anti-glial fibrillary acidic protein (GFAP ...
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bioRxiv - Microbiology 2021Quote: ... sBHI media controls or Porphyromonas cell-free supernatants (240 µg of total protein) were incubated with 120 µg of human fibrinogen (Sigma-Aldrich, St. Louis, MO). Reaction mixtures were incubated at 37°C under anaerobic conditions or in an atmosphere of 5% CO2 for cell suspension or cell-free supernatants ...
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bioRxiv - Immunology 2021Quote: TEER was measured in the human airway epithelium inserts to determine the integrity of tight junctions with a Millicell ERS-2 volt-ohmmeter (Millipore Sigma, Burlington, MA, USA). Briefly ...
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bioRxiv - Microbiology 2021Quote: Calu-3 cells (a human lung epithelial cell line; ATCC HTB-55) were maintained in Minimum essential medium Eagle (Sigma-Aldrich, Cat# M4655-500ML) containing 10% FCS and 1% PS.
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bioRxiv - Microbiology 2022Quote: ... Cells were resuspended in 100 μl of IntestiCult™ OGM Human with 10 µM Y-27632 and seeded in collagen IV (Sigma-Aldrich, cat#. C5533) pre-coated 96 well plates ...
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bioRxiv - Neuroscience 2022Quote: MDCKII cells in which the endogenous canine MDR1 (cMDR1) gene was replaced with a heterologous human MDR1 (hMDR1) (catalog number MTOX1303, Millipore Sigma, Burlington, Massachusetts, USA) were maintained in high glucose Dulbecco’s Modified Eagle Medium (catalog number 11965092 ...
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bioRxiv - Cancer Biology 2022Quote: ... cell death detection enzyme-linked immunosorbent assay PLUS (ELISA) and human anti-β-actin antibody were obtained from Sigma-Aldrich (Saint-Quentin-Fallavier, France). Poly-ADP-ribose polymerase (PARP ...
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bioRxiv - Cell Biology 2022Quote: ... the dishes were coated with a freshly made 2 ug/mL solution of fibronectin diluted in phosphate buffered saline (PBS) from a stock of 0.1% human plasma fibronectin (Sigma Aldrich, Cat. no. F-0895). The fibronectin coating was applied overnight at 4oC ...
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bioRxiv - Cell Biology 2022Quote: IMR-90 normal human fibroblasts (ATCC, CCL-186) and HEK293T cells (ATCC, CRL-3216) were cultured in DMEM (Sigma–Aldrich, D5796; Wako, 044-29765) supplemented with 10% FBS (BioWest ...
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bioRxiv - Microbiology 2022Quote: ... HOS cells stably expressing human ACE2 and TMPRSS2 (Ferreira et al., 2021; Ozono et al., 2021) were maintained in DMEM (high glucose) (Sigma-Aldrich, Cat# 6429-500ML) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The plasmid that contains the kinase domain of human HASPIN was transformed into Escherichia coli strain Rosetta™ 2(DE3) pLysS (Novagen, Madison, WI, USA). The transformed cells were grown at 37□ until OD600 reached 0.8 ...
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bioRxiv - Bioengineering 2024Quote: For podocyte stimulation the intact kidney was perfused through the arterial catheter with 250 µl of 10 µM human angiotensin 2 (Sigma-Aldrich, Darmstadt, Germany, A9525), dissolved in custom kidney buffer (10 mM NaHCO3 ...
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bioRxiv - Cell Biology 2024Quote: ... and rabbit polyclonal anti-β-actin antibody (catalog number A2066, epitope: amino acids 365-375 of human β-actin) were obtained from Sigma (Madrid, Spain). Mouse monoclonal Anti-GOK/STIM1 antibody (Clone 44/GOK ...