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Citations for Millipore Sigma :
3301 - 3350 of 3691 citations for CD40 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... in the presence of HODHBt or DMSO control and cultured in complete RPMI 1640 medium containing 10% human AB serum (Sigma-Aldrich). As a control ...
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[18F]DCP, First Generation PET Radiotracer for Diagnosis of Radiation Resistant Head and Neck CancerbioRxiv - Cancer Biology 2020Quote: The ex vivo serum stability of the radiotracer [18F]DCP was performed following previously published methods (55–57) and human serum (Sigma Aldrich). [18F]DCP (~1-2 mCi ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lines stably expressing shRNA to human FBXO7 were generated as described previously (33-35) and selected with 2 µg/mL puromycin (Sigma-Aldrich). To vary glucose concentration ...
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bioRxiv - Biochemistry 2021Quote: ... All wells were then incubated with 3,000-fold diluted goat anti-human IgG-alkaline phosphatase conjugate (Sigma-Aldrich, St. Louis, MO) for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: Free floating mouse brain sections were incubated with 300 nanograms of active human cathepsin-S (SRP0292, Sigma-Aldrich, St. Louis, MO), in activation buffer containing 1.8 mM DTT ...
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bioRxiv - Microbiology 2020Quote: ... One hundred microliters of secondary antibody (alkaline phosphatase-conjugated goat anti-rabbit IgG or goat anti-human IgG, both from Sigma-Aldrich) diluted 1:2,000 in 1% nonfat milk-PBS were then added to each well and the plates incubated for additional 3 h at room temperature before repeating the washing step ...
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bioRxiv - Systems Biology 2021Quote: ... purified LC and naïve CD4+ T cells or TRMs were co-cultured in human serum supplemented media (RPMI, Gibco, UK, 10% human serum, Sigma, UK, 100 IU/ml penicillin and 100 mg/ml streptomycin, Sigma, UK) at a 1:50 ratio for 5-days at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... 200 μl of bacterial suspension was added to 400 μl of pre-warmed at 37°C human sera (Sigma-Aldrich S7023), and the mixture was incubated for 2h at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... Then cells were resuspended to 100 μl PBSA buffer followed by addition of 1 μl PE conjugated anti-human Fc antibody (Sigma-Aldrich) and incubate at 4 °C for 30 min ...
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bioRxiv - Neuroscience 2020Quote: ... male and primed female frogs were injected with 150 IU and 400 IU of human chorionic gonadotropin (Sigma-Aldrich, Oakville, CA) into the dorsal lymph sac ...
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bioRxiv - Microbiology 2021Quote: Samples were diluted 1:2 in serum matrix for analysis with Milliplex Human Cytokine/Chemokine Magnetic Bead Panel as per manufacturer’s instructions (EMD Millipore Corporation). Concentrations for analytes (EGF ...
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bioRxiv - Microbiology 2021Quote: ... Parasites were maintained in vitro in human O+ erythrocytes at 4% haematocrit in RPMI 1640 medium supplemented with 25mM HEPES (Sigma-Aldrich), 0.25% sodium bicarbonate ...
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bioRxiv - Microbiology 2021Quote: ... 293T-ACE2 cells stably expressing human ACE2 are derived from 293T cells and were maintained in medium supplemented with 2 µg/mL of puromycin (Millipore Sigma) (Prévost et al. ...
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bioRxiv - Immunology 2021Quote: ... IgG-free BSA treatment and blocking prior to opsonization was omitted for beads and surfaces opsonized with human IgG (Sigma, I4506). Free active groups were blocked overnight with 0.5M Tris (pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... gondii tachyzoites were passaged onto Human foreskin fibroblasts (HFFs; ATCC, SCRC-1041) monolayers at 37 °C and 5% CO2 in DMEM (Sigma, D6546) supplemented with 10% FBS (BioSell FBS.US.0500) ...
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bioRxiv - Pathology 2021Quote: PLA was performed in cryostat sections (4–6 μm) obtained from frozen samples of human parietal cortex using the Duolink In Situ Detection Reagents Brightfield system (Sigma-Aldrich). This method monitors the coincidence of specific epitopes and has been previously used for monitoring protein phosphorylation (77) ...
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bioRxiv - Cancer Biology 2021Quote: Human MDA-MB-231 breast adenocarcinoma cells obtained from ATCC (ATCC HTB-26) were grown in L-15 medium (Sigma-Aldrich) supplemented with 15% fetal calf serum (FCS ...
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bioRxiv - Cancer Biology 2021Quote: Cytokine profiling was performed by the Human Immunology Core at the University of Pennsylvania using the Luminex FlexMap 3D system (EMD Millipore). A MILLIPLEX human cytokine 48-plex panel (Millipore Sigma HCYTA-60K-PX48 ...
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bioRxiv - Genomics 2021Quote: Specific antibodies recognizing human H1 variants used for ChIP/ChIP-seq were: anti-H1.0/H5 clone 3H9 (Millipore, 05-629-I), anti-H1.2 (Abcam ...
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bioRxiv - Cell Biology 2021Quote: GFP+/- LKS and GMPs were sorted as described above and live cells were imaged in 8 chamber borosilicate coverglass system (nunc) coated with human plasma fibronectin (EMD Millipore) using a Leica TCS SP8 confocal microscope equipped with two photomultiplier tubes ...
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bioRxiv - Cell Biology 2021Quote: HUVEC were seeded at 5×104 cells or 2×105 cells in 24-well plates on coverslips coated with 10 μg/ml fibronectin (from human plasma, Sigma Aldrich) and incubated overnight in complete EBM-2 medium ...
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bioRxiv - Cancer Biology 2020Quote: Established human glioblastoma cell line T98G (VTT Technical Research Centre, Turku, Finland in 2010) was cultured in Eagle MEM (Sigma-Aldrich), supplemented with 10% heat-inactivated FBS (Biowest) ...
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bioRxiv - Cancer Biology 2022Quote: ... and the Human Cytokine/Chemokine Magnetic Bead Panel (Cat# HCYTMAG-60K-PX30) on a Luminex MAGPIX system (Merck Millipore, Billerica, MA). Conditioned media concentrations (pg/mL ...
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bioRxiv - Cancer Biology 2022Quote: ... Radboud Institute for Molecular Life Sciences, Nijmegen, The Netherlands) and human fibroblasts (gift from Johanna Ivaska, Turku Bioscience, Turku, Finland) were cultured in DMEM (Sigma-Aldrich). T98G (VTT Technical Research Centre ...
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bioRxiv - Neuroscience 2022Quote: Two immortalized cell lines were used to validate the ACE2 receptor, TMPRSS2 and NRP1 assays: Human oligodendroglioma cells (HOG, Cat# SCC163) were obtained from Millipore Sigma-Aldrich (Billerica ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were stained with primary antibodies diluted with TBS + 2% BSA + 0.1% Triton X-100 at 1:1000 (human anti-ACA, Geisel School of Medicine at Dartmouth) and 1:4000 (mouse anti-α-tubulin, Sigma) following the standard immunostaining protocol as described above ...
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bioRxiv - Biochemistry 2022Quote: ... Then cells were stained with anti-mouse IgG Taxes red conjugated antibody and anti-human IgG FITC-conjugated antibody (Sigma, USA) for another 30 min then analyzed by FACS Aria II (BD ...
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bioRxiv - Neuroscience 2022Quote: 90,000 human NPCs were plated in a well of Chambered Coverglass wells that had been coated with Poly-L-Ornithine (Sigma Aldrich) and Laminin (Sigma Aldrich) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... human lung parenchyma and human pulmonary artery were finely minced with scissors in an enzyme cocktail of collagenase I (5mg/ml; Sigma, UK), DNase I (125U/ml ...
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bioRxiv - Molecular Biology 2022Quote: ... Multiplex cytokine profiling was carried out on a Luminex MAGPIX system (Luminex) using the Milliplex Human Premixed 41 Plex Immunology Multiplex panel (Millipore Sigma) following the manufacturer’s recommendations.
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bioRxiv - Microbiology 2022Quote: ... Calu-3 cells (a human lung epithelial cell line; ATCC HTB-55) were maintained in EMEM (Sigma-Aldrich, Cat# M4655-500ML) containing 20% FBS and 1% PS ...
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bioRxiv - Cancer Biology 2022Quote: ... The chopped and digested pieces were placed in a serrated 60mm petri dishes and supplemented with 10ng/μl each of human recombinant epidermal growth factor (hEGF, Cat # E9644, Sigma-Aldrich), N2 suppliment-1X (Cat # 17502048 ...
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bioRxiv - Microbiology 2022Quote: ... Aliquots of them were analyzed with the MILLIPLEX human cytokine/chemokine magnetic bead panel (HCYTOMAG-60K, Merck Millipore, Burlington, MA, USA) using the Luminex 200 multiplexing instruments (40-012 ...
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bioRxiv - Cancer Biology 2022Quote: ... and NCI-H3122 [H3122] (NCI DTP Developmental Therapeutics Program, male, human) cells were cultured at 37°C in RPMI medium (Sigma-Aldrich) supplemented with 10% FBS (PAN Biotech) ...
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bioRxiv - Cell Biology 2022Quote: ... Serial dilutions of the concentrated supernatants were then incubated with human neutrophil elastase (MilliporeSigma) in the presence of methoxysuccinyl-Ala-Ala-Pro-Val-p-nitroanilide (Millipore Sigma). To quantify bioactivity colorimetric change was quantified using a 96 well plate reader set at 405nm (54).
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bioRxiv - Immunology 2020Quote: Levels of nitrate and nitrite in plasma obtained from human and mice were estimated by a Griess colorimetric assay kit (Sigma chemicals) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: Mice were injected subcutaneously under anaesthesia into the right flank with 5 × 106 human bladder cancer cells (RT112) in RPMI medium (Sigma Aldrich) with phenol red-free Matrigel (BD Biosciences ...
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bioRxiv - Neuroscience 2020Quote: Mating between pairs of adult Xenopus laevis was induced regularly by injections of human chorionic gonadotropin (HCG, 1000 U/ml, Sigma, UK) into the dorsal lymph sacs ...
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bioRxiv - Molecular Biology 2020Quote: ... a single fluorescent cell was sorted per well of a fibronectin-coated (10 μg/mL Human Plasma Fibronectin Purified Protein (Sigma Aldrich) in 1x PBS ...
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bioRxiv - Neuroscience 2021Quote: ... Cell lines stably expressing shRNA to human FBXO7 were generated as described previously (20) and selected with 2 μg/mL puromycin (Sigma-Aldrich).
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bioRxiv - Microbiology 2020Quote: ... Chips were then washed thoroughly with PBS before incubating with an ECM solution of 150 μg/mL bovine collagen type I (AteloCell, Japan) and 30 μg/mL fibronectin from human plasma (Sigma-Aldrich) in PBS buffered with 15 mM HEPES solution (Gibco ...
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bioRxiv - Biochemistry 2021Quote: The competitive inhibition of human fumarase activity in the presence of 2-amino-3-phosphonopropionic acid (AP-3, Sigma-Aldrich A4910) was fluorometrically assessed using a coupled enzyme assay ...
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bioRxiv - Bioengineering 2021Quote: ... were coated with 100 μl (5 μg/ml) of purified S1 or S1-RBD or commercial human serum albumin (HSA; Sigma-Aldrich) and incubated overnight at 4°C ...
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bioRxiv - Bioengineering 2020Quote: ... 16 hours post transfection gene expression from the human CMV immediate-early gene enhancer/promoter was induced with 10 mM sodium butyrate (Sigma-Aldrich) for 6 hours before fresh media was added to the cells ...
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bioRxiv - Cancer Biology 2020Quote: Biotin labelled and unlabeled RNA oligonucleotides probes corresponding to the human cMYC ARE (1-6) and two random probes (R1, R2) were synthesized as indicated in Table S5 by Sigma-Aldrich. REMSA was performed with a LightShift chemiluminescent RNA EMSA Kit (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: Adipogenic differentiation of ASC was induced during 14 days with human adipocyte differentiation medium [DMEM/F12 50:50 and the following reagents from Sigma-Aldrich Inc. ...
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bioRxiv - Developmental Biology 2022Quote: ... A pair of adults were placed in a 10-gallon tank filled with 10% Holtfreter solution (Holtfreter, 1931) and injected with 10 international units (IU) of human chorionic gonadotropin (HCG; Sigma CG10) into the dorsal lymph sac ...
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bioRxiv - Genomics 2022Quote: ... Mouse gRNA constructs and pCMV-sadCas9-KRAB (kind gift from Dr. Alejandro Lomniczi OHSU) were co-transfected into human preadipocytes X-tremeGene (Sigma, 6366236001) while human gRNA plasmids and pCMV-sadCas9-KRAB were co-transfected into mouse 3T3-L1 preadipocytes ...
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bioRxiv - Molecular Biology 2022Quote: ... Secretion of the following cytokines was quantified with the MILLIPLEX® Human CD8+ T Cell Magnetic Bead Panel Premixed 17 Plex - Immunology Multiplex Assay kit (EMD-Millipore): IL-6 ...
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bioRxiv - Cancer Biology 2022Quote: ... 2-3 mm3 tumor pieces were cut and placed in a 24-well plate containing RPMI medium supplemented with 10% human AB serum (Sigma-Aldrich), 6000 IU/ml human recombinant IL2 (Peprotech) ...