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Citations for Millipore Sigma :
3501 - 3550 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 aa 1000 1200 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... The signal was detected by the Pro e-BLOT Touch Imager using chemiluminescent substrate (WBKLS0500; Merck Millipore, Boston), and the band intensity was measured using ImageJ software (National Institutes of Health).
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bioRxiv - Neuroscience 2020Quote: Mice were anesthetized using Avertin (2, 2, 2-tribromoethanol; Sigma-Aldrich, 476 mg/kg, i.p) and placed into a stereotactic frame (Kopf) ...
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bioRxiv - Neuroscience 2021Quote: Mice were anaesthetized using Avertin (2, 2, 2-tribromoethanol; Sigma-Aldrich, 476 mg/kg, i.p.) and were surgically implanted with a microdrive (manufactured with the assistance of the Advanced Manufacturing Support Team ...
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bioRxiv - Neuroscience 2020Quote: Mice were anaesthetized using Avertin (2, 2, 2-tribromoethanol; Sigma-Aldrich, 476 mg/kg, i.p.) and were surgically implanted with a microdrive (manufactured with the assistance of the Advanced Manufacturing Support Team ...
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bioRxiv - Cell Biology 2022Quote: ... and 2% glucose) or YPL (0.5% yeast extract, 2% peptone and 2% lactate (Sigma L1375)) ...
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bioRxiv - Bioengineering 2023Quote: Photoinitiator 2-hydroxy-1-[4-(2-hydroxyethoxy) phenyl]-2-methyl-1-propanone (HEPK, Sigma Aldrich) was dissolved into deionized water to obtain an approximately 0.077 wt% solution ...
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bioRxiv - Biophysics 2020Quote: ... biotin binding deficient subunits equipped with a C-terminal His6-tag – “dead” – and functional subunits with C-terminal 3C protease cleaving site followed by Glu6-tag – “alive” – were expressed as inclusion bodies in E.coli BL-21 using the pET expression system (Novagen). Inclusion bodies were dissolved in 6 M Guanidine hydrochloride at 10 mg/ml ...
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bioRxiv - Molecular Biology 2021Quote: ... Bound FLAG-HA-tagged KAT6B and associated proteins were eluted with 0.3 μg/μl 3x FLAG tag peptide (Sigma) in BC100 buffer for 1 h with gentle rotation at 4°C.
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bioRxiv - Synthetic Biology 2019Quote: ... the resin was incubated with 1 CV of 100 μg/ml 3x FLAG tag peptide (SIGMA F4799, Sigma-Aldrich) for 30 min at 4 °C ...
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bioRxiv - Synthetic Biology 2019Quote: ... the resin was incubated with 1 CV of 100 μg/ml 3x FLAG tag peptide (SIGMA F4799, Sigma-Aldrich) for 30 min at 4 °C ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... or these cells stably transfected with human CB1 or CB2 receptors with 3x N-terminus haemagglutinin tags (AtT20-CB1 and AtT20-CB2respectively).17 Cells were cultivated in Dulbecco’s Modified Eagle’s Medium (DMEM; Sigma-Aldrich) supplemented with 10% foetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2021Quote: ... Coding region of H3.1 along with Flag-Tag was amplified by PCR using KOD Hot Start DNA Polymerase (Millipore) and cloned into gateway entry vector pENTR/D-TOPO vector (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: Antibodies against the following proteins were obtained from commercial sources and used for immunoblots: anti-flag-tag (F3165, Sigma), anti-V5-tag (R960-25 ...
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bioRxiv - Biochemistry 2022Quote: ... CLP1 contained a C-terminal FLAG-Tag and the sample was purified using ANTI-FLAG M2 Affinity Gel (Sigma), eluted with Pierce 3X FLAG peptide (Thermo ...
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bioRxiv - Plant Biology 2019Quote: ... a C-terminal polypeptide encompassing the last 284 amino acids of the PKL protein has been expressed in fusion with a hexahistidine tag in the E.coli strain BL21DE3 Rosetta (Novagen). The protein was purified under denaturing conditions on Ni2+-nitrilotriacetic acid matrix (Ni-NTA Agarose ...
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bioRxiv - Microbiology 2020Quote: ... Samples were clarified by centrifugation and incubated with anti-Myc tag antibody conjugated agarose (Millipore, Cat. no. 16-219) for 3h ...
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bioRxiv - Microbiology 2020Quote: ... was used for SDS-PAGE and Western blotting in Tris-buffered saline and 0.2% Tween 20 (TBST) using Strep-Tag II HRP (1:2,500; EMD Millipore) and rabbit anti-vinculin (1:1,000 ...
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bioRxiv - Immunology 2020Quote: ... codon-optimized Bl-Eng2 open reading frame with an upstream enterokinase site and downstream c-Myc and 6X histidine tags was cloned into the SpeI and XhoI sites of the pET43.1b plasmid (Novagen); this forms a fusion protein with an upstream NusA tag ...
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bioRxiv - Microbiology 2022Quote: ... incubated 1 hour with primary mouse monoclonal antibody anti-HA tag (G036, Abm Inc.) diluted 1:10,000 and then probed with horseradish peroxidase-labeled anti-mouse immunoglobulin (A9044, Sigma), diluted 1:15,000 ...
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bioRxiv - Molecular Biology 2022Quote: ... Subsequent GST-tag cleavage was carried out under rotation overnight at 4°C (Thrombin from bovine plasma, Sigma-Aldrich) followed by secondary GST-purification to remove free GST tag (untagged Mcl-1 collected in flowthrough) ...
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bioRxiv - Cell Biology 2024Quote: ... Primary antibodies include: anti-V5 Tag (Invitrogen, ref R96025, 1:500, anti-HA (Sigma-Aldrich, ref H6908, 1:500), anti-Akt (pan ...
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bioRxiv - Immunology 2023Quote: ... Flag-tagged CD45 constructs in DP10.7 Jurkat T cell transductants were stained with 10ug/ml anti-Flag-tag polyclonal antibody (Sigma) for 1hr,
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bioRxiv - Biochemistry 2023Quote: ... Y730F)41 was cloned into pET28a vector incorporating a His6-tag and a SUMO tag at the amino terminus of the expressed protein and expressed in E.coli BL21 gold strain (Novagen). Cells were grown at 37°C in TB medium containing 0.1 mg/ml kanamycin and 0.050 mg/ml tetracyline to an OD600 of ∼3.0 ...
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bioRxiv - Cell Biology 2022Quote: ... for Strep-tagII::MLC-4 detection or mouse anti-6xHistidine tag antibody (Millipore clone HIS.H8, 1:2,500, 05-949) for 6xHIS::NMY-2 detection ...
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bioRxiv - Developmental Biology 2022Quote: TAG determination: 60 μl of each sample were mixed with 60 μl of Triglyceride Reagent (Cat. # T2449, Sigma-Aldrich), incubated at 37 °C for 60 min ...
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bioRxiv - Biochemistry 2023Quote: ... the protein (with N-terminal His6 tag) was concentrated using a centrifugal filter (Amicon Ultra, MWCO 30 kDa, Sigma) to 8.11 mg/ml ...
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bioRxiv - Molecular Biology 2023Quote: Full length Xenopus laevis Polκ with an N-terminal 6xHis-tag was amplified from pCMV-Sport.ccdb-Polκ (Williams et al., 2012) and cloned into pET28b (Novagen) using primers A and B ...
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bioRxiv - Biochemistry 2024Quote: ... anti-p-Histone H2A.X Ser139 (ψH2A.X, mouse monoclonal, 05-636) and pan-ADP-ribose binding reagent (rabbit Fc tag, MABE1016) were obtained from Millipore. anti-DHFR (rabbit polyclonal ...
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bioRxiv - Biochemistry 2024Quote: ... Different SRSF3 variants were subcloned in pET28a and expressed as TEV protease-cleavable N-terminal 6xHis-tag fusions in E.coli Rosetta2(DE3) cells (Novagen). E.coli cells were grown in TB media up to an OD600 of ∼ 1.2 at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies raised against AnkyrinG (1:1000; 75-146, NeuroMab, USA) and NeuN (1:1000, Millipore EMD, UK) were applied in a solution containing 5% normal goat serum ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Incubation with primary antibodies (anti-NvNrxnα3-1 -1:1000; anti-alpha-tubulin - 1:1000 (Sigma Aldrich, cat. T6074)) was performed overnight at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... mouse anti-Flag (Sigma, F1804, 1:1000) and rabbit anti-HA (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... anti-MICU1 (#HPA037479 1:1000, Sigma-Aldrich), anti-MICU2 (#ab101465 1:1000 ...
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bioRxiv - Cancer Biology 2021Quote: ... mouse anti-Flag (Sigma #F1804, 1:1000); mouse anti-Akt (Cell Signaling #2920 ...
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bioRxiv - Cancer Biology 2021Quote: ... anti-Fibronectin rabbit polyclonal (1:1000, Sigma) and anti-actin mouse monoclonal antibody (1:2000 ...
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bioRxiv - Cancer Biology 2021Quote: ... anti-Sox4 (#AB5803, 1:1000) (EMD Millipore), anti-BMPR1A (#ab174815 ...
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bioRxiv - Cell Biology 2021Quote: ... anti-phospho-PDHA (1:1000, Millipore #AP1062), anti-PDHA (1:1000 ...
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bioRxiv - Biophysics 2021Quote: ... 1000 units of catalase (Millipore Sigma, #C100), 70 units of glucose oxidase (Millipore Sigma ...
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bioRxiv - Cancer Biology 2021Quote: ... and anti-β-Actin (Sigma 1:1000) and secondary antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... rabbit anti-Sox9 (1:1000, Millipore, AB5535), goat anti-Klf5 (1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... rat anti-HA (Sigma; 1:1000 dilution), and mouse m2 anti-FLAG (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... anti-synapsin I (S193; Sigma;1:1000), anti-Prominin (SAB4300882 ...
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bioRxiv - Cell Biology 2020Quote: ... anti-Prominin (SAB4300882; Sigma-Aldrich; 1:1000) (Sigma Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... rabbit α-pH3 (D5692, 1:1000; Sigma), and guinea pig α-HTP-3 (1:200 ...
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bioRxiv - Genetics 2021Quote: ... anti-p-PKD2 (ser876) (Sigma, 1:1000), anti-PKD3 (Cell signaling technology ...
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bioRxiv - Developmental Biology 2021Quote: ... Anti-FLAG (F1804/F3165, Sigma, 1: 1000), anti-MYC antibody (Transgen Biotech ...
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bioRxiv - Developmental Biology 2021Quote: ... and DAPI (1:1000 dilution – Sigma Aldrich) stained sections ...
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bioRxiv - Neuroscience 2021Quote: ... pH3 (1:1000; Millipore, CAT#: 06-570), Ki67 (1:1000 ...
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bioRxiv - Developmental Biology 2021Quote: ... and Hoechst 34580 (Sigma, 63493, 1:1000) were used ...
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bioRxiv - Developmental Biology 2021Quote: ... and 1000 U/ml ESGRO (Millipore, ESG1106) to obtain a single cell suspension (1.5×105 cells/ml) ...