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Citations for Millipore Sigma :
3401 - 3450 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 aa 1000 1200 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Proteins were eluted by cleavage of the GST tag by 15 units/ml TEV protease (Sigma T4455-1KU) in PBS buffer overnight at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Strains with degradation tags were treated with 250 µM 3-indole acetic acid (Sigma-Alrich, St. Louis, MO) in DMSO and 50 mM potassium phosphate buffer ...
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bioRxiv - Cell Biology 2021Quote: ... was expressed in COS-7 cells and immobilized to anti-T7 tag-antibody-conjugated agarose beads (EMD Millipore) (Matsui et al. ...
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bioRxiv - Cell Biology 2021Quote: ... Blood levels of TAGs were measured by a colorimetric assay using the Triglyceride determination kit (Sigma-Aldrich, #MAK266). LCN2 plasma levels were determined by ELISA (R&D Systems ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The protein composition of the eluent was verified by western blot using Strep-tag II monoclonal antibodies (Novagen). The fractions containing MGE protein were pooled and concentrated to 1 mL by using a centrifugal concentrator (Sartorius ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The protein composition of the eluent was verified by western blot using Strep-tag II monoclonal antibodies (Novagen). The fractions containing MGE protein were pooled and concentrated to 1 mL by using a centrifugal concentrator (Sartorius ...
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bioRxiv - Molecular Biology 2021Quote: ... tags and TEV protease were removed by an additional round of affinity chromatography on Ni-NTA-agarose (Sigma). As a final step gel filtration on a Superdex 75 column in 20 mM phosphate buffer ...
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bioRxiv - Microbiology 2022Quote: ... The membrane was subjected to Western blot analysis using a polyclonal antibody against the 6Histidine tag (Sigma-Aldrich). After incubation with the second anti-mouse antibody (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: The Cleavage Under Targets and Tagmentation (CUT&Tag) experiments were performed using FLAG antibodies (Flag, Sigma, F1804/F3165) in 3× Flag-POGZ restoring Pogz−/− mESCs by the Yingzi and Frasergen Companies (Wuhan ...
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bioRxiv - Microbiology 2023Quote: ... 3% BSA overnight at 4 □C (rabbit α-LC3B 1:1000 – Cell Signaling/ #4108, rabbit α-Atg5 1:1000 – Cell Signaling/#2630, mouse α-tubulin 1:1000 – Sigma Aldrich/T5168). After 3 washes in TBST the membranes were incubated in secondary antibodies for 2 h at room temperature (Goat α-rabbit HRP 1:1000 - Sigma Aldrich/ GERPN4301 ...
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bioRxiv - Neuroscience 2023Quote: ... incubated overnight at 4° C (MAP2 1:1000 Invitrogen #MA5-12826, GABA 1:1000 Sigma Aldrich #A2052). Cultures were washed 2X with D-PBS and blocking performed using 5% donkey serum for 10 min ...
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bioRxiv - Immunology 2020Quote: ... in a bead-to-cell ratio of 0.5:1 in simultaneous presence or absence of 2 mM 2-Fluoro-2-deoxy-D-glucose (2-FDG) (Sigma, F5006) at 37°C ...
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bioRxiv - Neuroscience 2022Quote: Mice were anesthetized with avertin (2, 2, 2-tribromoethanol, Sigma Aldrich, St. Louis, MO) or isoflurane for stereotaxic surgery (Model 1900 Stereotaxic Alignment System ...
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bioRxiv - Immunology 2023Quote: ... 10 µM N-acetylcysteine (NAC) or 2 mM 2-desoxyglucose (2-DG, both Sigma) were added during cultivation ...
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bioRxiv - Immunology 2023Quote: ... After general anesthesia with i.p.-administered Avertin (2, 2, 2-tribromoethanol, Sigma-Aldrich #T48402) the blood was collected with heparinized capillaries (Fisher Scientific™ #22-260950 ...
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bioRxiv - Biochemistry 2023Quote: ... Irgacure 2959 (2-Hydroxy-4′-(2-hydroxyethoxy)-2-methylpropiophenone) were purchased from Sigma Aldrich for preparation of crosslinked polymer.
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bioRxiv - Immunology 2021Quote: ... coli O55:B5 that does not contain sialic acid was purchased from Sigma Aldrich (cat# L2880).(Lindberg et al ...
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bioRxiv - Microbiology 2020Quote: ... coli DH10B pks− were seeded in a 96-well plate in LB liquid medium (Sigma-Aldrich). The optical density (OD ...
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bioRxiv - Molecular Biology 2021Quote: Mouse placental samples: Samples were obtained using the intraperitoneal injection LPS (Escherichia coli O111:B4; Sigma) model that results in 100% preterm labor/birth [40] ...
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bioRxiv - Immunology 2019Quote: ... mice were treated with 15 mg/kg BW LPS (Escherichia coli serotype O55:B5; Sigma-Aldrich) with or without 10 mg/kg BW M3 and were evaluated twice daily for 7 days.
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bioRxiv - Neuroscience 2022Quote: ... lipopolysaccharide (LPS; Escherichia coli, serotype 026:B6; L-3755, Sigma, St. Louis, MO, USA; n = 7) or pyrogen-free saline (saline ...
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bioRxiv - Microbiology 2022Quote: Wild type and mutant CANC proteins were expressed in Escherichia coli Rosetta (DE3) cells (Merck Millipore) as previously described (Betancor et al. ...
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bioRxiv - Microbiology 2022Quote: ... coli MG1655 growing in LB were treated with 100 μg/ml Cephalexin (Sigma-Aldrich, Mumbai, India) for 2 hours ...
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bioRxiv - Plant Biology 2021Quote: ... The resultant expression vector was transformed into Escherichia coli BL21 (DE3) cells (Sigma-Aldrich, MO, USA).
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bioRxiv - Neuroscience 2020Quote: 330 µg/kg LPS (from Escherichia coli serotype 0111:B4, Sigma-Aldrich, St. Louis, MO, USA) dissolved in 0.9% sterile saline or 0.9% sterile saline control (SAL ...
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bioRxiv - Microbiology 2020Quote: THP-1 cells were treated with 100 ng/mL LPS from Escherichia coli (Sigma-Aldrich – L6895) or media only (mock treatment ...
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bioRxiv - Cell Biology 2019Quote: ... the other received LPS (1μg/5μl; Escherichia coli O55:B5 Sigma-Aldrich; St. Louis, MO, USA) according to protocols previous optimized for volume (16 ...
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bioRxiv - Genetics 2021Quote: BMDM or ESDM were primed with 1μg/mL LPS from Escherichia coli O55:B5 (Sigma, L6529) for 4 hours at 37°C and subsequently treated with 5mM adenosine triphosphate (ATP ...
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bioRxiv - Microbiology 2020Quote: ... coli and Lactobacillus was isolated using the GeneElute™ Bacterial Genomic DNA Kit (Sigma-Aldrich, USA) as described in the user manual ...
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bioRxiv - Immunology 2020Quote: Lipopolysaccharide from Escherichia coli 055:B5 (purified by gel-filtration chromatography) was purchased from Sigma-Aldrich and freshly dissolved in sterile saline prior to intraperitoneal (i.p. ...
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bioRxiv - Microbiology 2022Quote: ... Escherichia coli strain DH5-α was cultured shaking in Luria-Bertani broth (Lennox) (LB, Sigma-Aldrich) at 37°C ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... coli BL21 (DE3) cells were transformed with split intein cMSP1D1 constructs inserted into pET28a vector (Novagen) in LB media + kanamycin ...
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bioRxiv - Biochemistry 2023Quote: MFF-ΔTM was expressed in RosettaTM2 BL21-(DE3) Escherichia coli (71400; EMD Millipore Corporation, Burlington, MA) in LB broth ...
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bioRxiv - Molecular Biology 2023Quote: ... coli N-terminal domain (NTD; residues 1-130) of ProQ were cloned into pET15b vector (Novagen), as described (Stein et al ...
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bioRxiv - Biochemistry 2023Quote: ... Plasmids were transformed into Escherichia coli BL21(DE3) CodonPlus cells and grown in Terrific Broth (Sigma) at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... coli were adapted to grow in 2.5 mM paraquat (methyl viologen dichloride hydrate, Sigma Aldrich 856177) following a modified adaptive evolution protocol 52,53 ...
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A synthetic elastic protein as molecular prosthetic candidate to strengthen vascular wall elasticitybioRxiv - Cell Biology 2023Quote: ... coli bacteria selected on Luria Bertani (LB) both-agar plates containing 50 µg/mL kanamycin (Sigma–Aldrich ...
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bioRxiv - Biophysics 2023Quote: ... Subsequently the expression vector was transformed into and overexpressed in Escherichia coli BL21 (DE3) cells (Novagen). The recombinant WT and mutant hSOD1 proteins were all found in inclusion body ...
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bioRxiv - Cell Biology 2023Quote: MFF-ΔTM was expressed in RosettaTM2 BL21-(DE3) Escherichia coli (71400; EMD Millipore Corporation, Burlington, MA) in LB broth ...
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bioRxiv - Bioengineering 2024Quote: ... 1 μg/mL Escherichia coli lipopolysaccharide (M1 polarizing positive control; Sigma-Aldrich, Cat. No. L3024-5mg), 20 ng/mL mouse IL-4 (M2 polarizing positive control ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli was grown in LB media (LB broth, Miller) with kanamycin (Millipore Sigma, 50 µg/mL).
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bioRxiv - Neuroscience 2021Quote: ... Microtubule-Associated Protein 2 (MAP-2) (Merck Millipore Burlington ...
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bioRxiv - Developmental Biology 2022Quote: ... 2 mM 2-Deoxy-D-glucose (Sigma-Aldrich), and 1 mM Sodium azide ...
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bioRxiv - Biophysics 2022Quote: ... 0.1% 2-mercaptoethanol (60-24-2, Sigma-Aldrich), and 0.1% leukaemia inhibitory factor (LIF ...
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bioRxiv - Plant Biology 2022Quote: ... 2 mM CaCl2 + 2 mM EGTA (Sigma MFCD00004291), 500 μM MgCl2 in transparent flasks ...
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bioRxiv - Cell Biology 2019Quote: ... supplemented with 2 nM BMP-2 (Sigma-Aldrich) for two weeks ...
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bioRxiv - Synthetic Biology 2019Quote: ... 2-hydroxyethyl 2-bromoisobutyrate (HEBIB, Sigma-Aldrich, 95%), poly(ethylene glycol ...
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bioRxiv - Neuroscience 2019Quote: ... dissolved in 2-methyl-2-butanol (Sigma Aldrich) 2.5% v/v)] ...
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bioRxiv - Immunology 2022Quote: ... 2-Amino-2-norbornanecarboxylic acid (BCH; Sigma, #A7902), α-(Methylamino)isobutyric acid (MeAIB ...
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bioRxiv - Immunology 2022Quote: ... 100 mM 2-deoxyglucose (2-DG) (Sigma-Aldrich). Non-glycolytic acidification of media (ECAR following injection of 2-DG ...