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Citations for Millipore Sigma :
3501 - 3550 of 10000+ citations for 6 Chloro N methyl pyrimidine 4 5 diamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... and blocked with 5% milk in TBS-Tween 20 for 2h and incubated with primary antibodies diluted in the 5% BSA in TBS-Tween 20 overnight at 4°C (AMPD2, Sigma HPA045760 ...
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bioRxiv - Developmental Biology 2024Quote: ... Tg(-3.5ubb:loxP-GFPloxP-mCherry)cz1701Tg embryos were incubated in embryo media containing 5 μM (Z)−4-Hydroxytamoxifen (Sigma-Aldrich H7904) starting at 4-4.5 hours post-fertilization (hpf ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were reconstituted in 100 µL and 2 µL of the sample was injected from a 4 °C autosampler into a ZIC-pHILIC 150 × 2.1 mm 5 µm particle size column (EMD Millipore) with a ZIC-pHILIC 20 x 2.1 guard column in a Vanquish Duo UHPLC System (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... The protein expression of the mouse or human 5-HT3A was induced by the addition of 4 μg/mL tetracycline (Sigma). Valproic acid (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... 537-98-4] were added to the media from 25 mg/mL stock (in ethanol [Sigma Aldrich, 64-17-5]), and the final ethanol concentration was adjusted to 1.86% v/v for all conditions ...
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bioRxiv - Cell Biology 2023Quote: ... Slides were washed in 1xPBS for 5 minutes and coverslipped with 75µl of mounting media (50% glycerol – Sigma, G5516 and 4% propyl gallate – Sigma, 2370).
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bioRxiv - Immunology 2022Quote: ... RT complete IntestiCult containing 25μg/ml was added, and enteroids were incubated (37°C, 5% CO2) until fixation with 4% paraformaldehyde (PFA; Sigma Aldrich) at 4h p.i ...
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bioRxiv - Microbiology 2023Quote: ... and remaining lysate was end-over-end incubated with 5 µg of primary Ab overnight at 4°C [Rabbit-anti-HA (Sigma), Rabbit-anti-Flag (Sigma) ...
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bioRxiv - Developmental Biology 2023Quote: ... 4- to 5-week-old donor rats were intraperitoneally injected with 30 units of equine chorionic gonadotropin (G4877, Sigma-Aldrich), followed by an intraperitoneal injection of 30 units of human chorionic gonadotropin (C1063 ...
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bioRxiv - Neuroscience 2023Quote: ... slices were washed 5 times in PB 0.1 M and mounted with a mounting solution (Mowiol 4-88, Sigma-Aldrich).
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bioRxiv - Immunology 2023Quote: ... CD11c-DTR/GFP BM chimeric mice were injected (i.p.) with 4–5 ng/g body weight diphtheria toxin (DT, Sigma-Aldrich) every other day for 14 days.
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bioRxiv - Biochemistry 2023Quote: ... Excess TMT reagent was quenched by the addition of 4 µl of an aqueous solution of 5% hydroxylamine (Sigma, 438227). Peptides were mixed to achieve a 1:1 ratio across all TMT-channels ...
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bioRxiv - Cell Biology 2022Quote: ... The conditioned media was spun down for 5 minutes at 800g and 4°C and then filtered with a syringe and 0.22 μm filter (Millipore, SLGV033RS) and stored at 4°C for no more than three days before being applied to cells ...
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bioRxiv - Cell Biology 2023Quote: ... adipocytes were lysed in polysome lysis buffer (20 mM HEPES-KOH pH 7.4, 100 mM KCl, 5 mM MgCl2, 1% Triton X-100, 2 mM DTT, 100 μg ml-1 cycloheximide [Sigma] ...
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bioRxiv - Genetics 2022Quote: ... K562 cells were transduced at a cell concentration of 5×105 cells/ml in the culture medium supplemented with 4 μg/ml polybrene (Sigma). After 24 h ...
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bioRxiv - Molecular Biology 2023Quote: The sorted cells were concentrated by centrifugation at 300 x g for 5 minutes at 4 °C and resuspended in DPBS (Sigma) with 0.04% w/v BSA (Sigma) ...
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bioRxiv - Immunology 2022Quote: 8 to 15-weeks old female C57BL/6j CD45.2+ mice were inoculated subcutaneously with 5×105 B16-F10-OVA and conditioned 4 days later with peritoneal injection of 300 mg/kg cyclophosphamide (Sigma). On day 7 ...
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bioRxiv - Developmental Biology 2023Quote: ... and then incubated overnight at 4 ℃ with primary antibodies diluted in 5% goat serum in PBS: phospho-histone H3 Ser10 (Millipore, Burlington ...
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bioRxiv - Systems Biology 2023Quote: ... All membranes were blocked in a 5% milk solution and then incubated for 12h at 4°C with either the mouse anti-phosphotyrosine 4G10 (05321, Millipore) or the rabbit anti-GFP (Thermo A11122 ...
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bioRxiv - Biophysics 2023Quote: ... PIEZO1 and Gardos ion channels were inhibited with 5 µM GsMTx-4 (Alomone Labs) and 10 µM TRAM34 (Sigma-Aldrich), respectively ...
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bioRxiv - Microbiology 2023Quote: ... The insoluble matter was removed by centrifugation 10000×g for 5 min at 4 °C and protein was estimated using BCA kit (Sigma) according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... The solubilized nucleoproteins were cleared by centrifugation at 20,400xg for 5 min at 4°C followed by filtration using an Ultrafree-MC spin column (Millipore, UFC30GV0S).
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bioRxiv - Cell Biology 2023Quote: ... The lysate was clarified by centrifugation at 20,000 × g for 5 min at 4°C and filtered on an Ultrafree-MC GV Centrifugal filter (Millipore, UFC30GV0S). Superose 6 Increase HR 10/300 column (Cytiva ...
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bioRxiv - Cell Biology 2023Quote: ... 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR) and the AhR inhibitor CH-223191 were purchased from Millipore Sigma (St. Louis, MO). Indole-3-acetate sodium salt (I3A ...
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bioRxiv - Biophysics 2023Quote: ... product of fusion protein Bre1(591-700) -(GGS)4- Bre1(591-700) -(GGS)5-Rad6(1-172) was cloned into vector pET32-a (Novagen) with N-terminal thioredoxin ...
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bioRxiv - Neuroscience 2024Quote: ... for 5 minutes and washed 3-4 times in PBS before being mounted in Mowiol mounting media (Millipore, 475904-M).
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bioRxiv - Cell Biology 2024Quote: ... whole seedlings were ground in Eppendorf tubes using a pellet pestle and total proteins were extracted in SDS extraction buffer (40 mM Tris-HCl pH 7.5, 10% glycerol, 5 mM MgCl2, 4% SDS, 1x protease inhibitor cocktail (Sigma-Aldrich), 1 mM phenylmethanesulfonyl fluoride) ...
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bioRxiv - Cancer Biology 2024Quote: ... ∼2 to 5 μg genomic DNA in 50 μL of HPLC grade water was digested with 4 Units of Nuclease P1 (Sigma) at 65°C for 10 minutes in a digestion buffer containing 0.04 mM desferrioxamine mesylate (DFAM) ...
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bioRxiv - Microbiology 2024Quote: ... Cell pellets were resuspended in 0.4 ml of sonication buffer (20 mM Tris pH 8.0, 5 mM EDTA, 1x EDTA-free protease inhibitors [Sigma Aldrich]) and subjected to sonication at 4.5-micron amplitude for 7 cycles of 15 s on / 15 s off ...
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bioRxiv - Microbiology 2024Quote: ... 4 °C), resuspended in 5 ml lysis buffer (150 mM NaCl, 50 mM Tris-HCl, 0.5ξ CellLytic B (Sigma-Aldrich), 1 % Triton X 100 ...
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bioRxiv - Immunology 2024Quote: ... at 37°C in pre-warmed epithelial cell removal solution (HBSS supplemented with 4% iHS [Sigma] and 5 mM EDTA [Sigma]). The remaining tissues containing lamina propria and muscularis layer were digested for 20 min digestion ...
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bioRxiv - Systems Biology 2024Quote: ... Cells were cultivated under humidified conditions with 5% CO2 at 37°C and passaged every 3-4 days using 0.05% trypsin/EDTA (Sigma-Aldrich). Cells were harvested in ice-cold PBS buffer using a cell scraper ...
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bioRxiv - Neuroscience 2021Quote: ... The left MFB was injected with either 1μL of 6-Hydroxydopamine hydrochloride (6-OHDA, (Sigma-Aldrich) 3.2µg/µL free-base in a 0.02% ascorbic acid solution (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: Serum IL-6 levels were quantified using a commercial mouse IL-6 ELISA kit (RAB0308, Sigma) according to the manufacturer’s instructions and analyzed using spectra-iMAX (Molecular Devices).
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bioRxiv - Cell Biology 2021Quote: ... 6 mM ATP or 6 mM ADP (conjugated with sodium salt; Sigma-Aldrich, St. Louis MO) were included along with the contents described above ...
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bioRxiv - Biophysics 2021Quote: ... 0.3% 10 mg/ml N-hydroxysuccinimide (NHS, Sigma Aldrich) in dimethyl sulfoxide (DMSO ...
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bioRxiv - Biophysics 2021Quote: ... 0.3% 10 mg/ml N-hydroxysuccinimide (NHS, Sigma Aldrich) in dimethyl sulfoxide (DMSO ...
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bioRxiv - Cell Biology 2020Quote: ... (cat n. R0883, Sigma-Aldrich, St. Louis, MO, USA), supplemented with 10% heat-inactivated FBS ...
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bioRxiv - Cell Biology 2020Quote: For the combined N-acetylcysteine (NAC, Sigma-Aldrich, #A7250) and formaldehyde (FA ...
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bioRxiv - Microbiology 2021Quote: ... and 100µL of 0.25 N Folin reagent (Sigma-Aldrich) was added to each tube ...
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bioRxiv - Developmental Biology 2021Quote: ... embryos develop in N-Phenylthiourea (PTU, Sigma-Aldrich, P7629) in Volvic source water (0.003% final) ...
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bioRxiv - Physiology 2022Quote: Liver triglycerides were measured using triglyceride determination kit from Sigma (catalog n°TR0100, Sigma-Aldrich, Saint-Louis, MO, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... 0.3% Triton X-100 (Sigma Aldrich, Cat. N: X100). Staining was performed overnight at 4°C with anti-Pax6-A488 (1:200 ...
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bioRxiv - Molecular Biology 2019Quote: ... and 20 mM deSUMOylation inhibitor N-Ethylmaleimide (Sigma, E3876). For Piwi/Arx/Panx coIP with Su(var)2-10 the lysis buffer contained 0.4% NP40 ...
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bioRxiv - Biochemistry 2019Quote: N-Acetyl-D-mannosamine (Sigma-Aldrich, cat. no. A8176)
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bioRxiv - Biochemistry 2019Quote: N-Acetyl-D-galactosamine (Sigma-Aldrich, cat. no. A2795)
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bioRxiv - Biochemistry 2019Quote: N-Acetyl-D-glucosamine (Sigma-Aldrich, cat. no. A8625)
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bioRxiv - Neuroscience 2020Quote: ... 5µg/mL N-Acetyl-L-cysteine (NAC, Sigma, A8199), 1x SATO and 5ng/mL HB-EGF (Peprotech ...
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bioRxiv - Cell Biology 2021Quote: ... 1.25 mM N-Acetyl-L-Cysteine (Sigma, #A9165-5G)) ...
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bioRxiv - Bioengineering 2022Quote: ... N’-methylene bis-acrylamide (2% wt/vol, Sigma Aldrich) were mixed with distilled water [25] ...