Labshake search
Citations for Millipore Sigma :
3301 - 3350 of 10000+ citations for 6 Chloro N methyl pyrimidine 4 5 diamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2019Quote: ... The lysate was centrifuged in a Beckman JA25.50 rotor at 16,000 rpm for 30 min at 4°C and 8.4 mL 5% neutralized PEI (Sigma P3143) was added dropwise into the supernatant with gentle agitation for 15 minutes at 4°C followed by another centrifuge at 13,000 rpm for 10 min at 4°C ...
-
bioRxiv - Physiology 2020Quote: The reduction of yellow tetrazolium salt 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; Sigma-Aldrich, Germany) was used to measure cellular metabolic activity as a proxy for cell viability [19,22] ...
-
bioRxiv - Neuroscience 2019Quote: ... The spinal cords were dissected and fixed in 4% paraformaldehyde (PFA) and 5% saturated picric acid (Sigma-Aldrich, P6744) in phosphate buffer saline (PBS ...
-
bioRxiv - Microbiology 2021Quote: ... 35x volume per mg) by vortexing vigorously for 5 min using 4 mm glass beads (ColiRollersTM Plating beads, Novagen), followed by incubation in a sonication bath for 10 min and shaking at 1,200 rpm for 10 min in a Thermomixer with a thermo block for reaction tubes ...
-
bioRxiv - Cancer Biology 2021Quote: ... fixed in 10% buffered formalin (4% formaldehyde) for 5 min at room temperature and permeabilized for 30 min in PBS containing 0.1% saponine (Sigma) and 1% FBS (HyClone) ...
-
bioRxiv - Microbiology 2020Quote: ... and membrane blocked overnight while shaking at 4°C in 5% milk (Marvel) containing 0.077% sodium azide (Sigma Aldrich). Primary antibodies were diluted in 5% milk/PBS/0.1% TWEEN 20 at concentrations as follows ...
-
bioRxiv - Plant Biology 2021Quote: ... Pellet was washed twice with acetone at maximum speed (13,000xg) for 5 minutes at 4◦C and then air dried and resuspended in 50 mM ammoniumbicarbonate (ABC) (Sigma).
-
bioRxiv - Microbiology 2021Quote: ... Cells were then incubated at 37 °C and 5% CO2 for 4 days before addition of resazurin (Sigma Aldrich) to a final concentration of 20 μg/mL ...
-
bioRxiv - Developmental Biology 2022Quote: ... gonads were dissected from 4 and 5 day old female jellyfish in 400 µM menthol in seawater (Sigma-Aldrich, #M2772 ...
-
bioRxiv - Bioengineering 2022Quote: ... the medium was changed to RPMI + B27-I with 5 μM Wnt inhibitor IWP-4 (SML1114, Sigma-Aldrich, USA) and incubated for 48 hours ...
-
bioRxiv - Developmental Biology 2022Quote: ... hiPSCs were plated at a density of 4×104 to 5×104 cells/cm2 onto a human fibronectin (Millipore)-coated 12-well plate in GK15 medium (Life Technologies ...
-
bioRxiv - Neuroscience 2020Quote: ... followed by a 4-hour decalcification in the same fixative with addition of 5 % EDTA (ethylenediaminetetraacetic acid, Sigma-Aldrich) at 4 °C.
-
bioRxiv - Neuroscience 2021Quote: ... Dehydrated tissue was then bleached with gentle shaking at 4°C for 48 hours in 1.5 ml of 5% H2O2 (Sigma) in methanol (1 volume of 30% H2O2 in 5 volumes of 100% methanol ...
-
bioRxiv - Microbiology 2021Quote: ... centrifuged for 5 min at 16,000 g at 4°C and homogenized with glass beads (≤ 106 μm; G4649, SIGMA) in 350 μl of lysis buffer (RNeasy Plus Mini Kit ...
-
bioRxiv - Cancer Biology 2020Quote: ... Conditioned media were retrieved by spinning down at 2,000 × g for 5 minutes at 4 °C then supernatants were passed through 0.22 μm filter (Millipore: SLGP033RS).
-
bioRxiv - Molecular Biology 2021Quote: ... The unbroken cells were removed by centrifugation (3,000 x g; 4°C; 5 min; Sigma 3-16KL; rotor 11180). The membrane fraction was pelleted down by high-speed centrifugation (100,000 x g ...
-
bioRxiv - Biophysics 2019Quote: ... The eluate was incubated for 3 h at 4°C with 5 mL of FLAG M2 Agarose beads (Sigma), collected in a glass column ...
-
bioRxiv - Immunology 2022Quote: ... overnight at 37°C in 5% CO2 tissue culture incubator and were subsequently fixed with 4% paraformaldehyde (Sigma-Aldrich) in DPBS for 30 minutes at RT ...
-
bioRxiv - Molecular Biology 2019Quote: ... 1000 rpm continuous shaking at 4 °C) with 100 μL elution buffer (6.7 mM m6A 5-monophosphate sodium salt [Sigma # M2780-10MG] ...
-
bioRxiv - Microbiology 2021Quote: ... Intracellular ions were extracted from dried pellets by overnight incubation at 4°C in 5 ml milliRho water (Millipore). Na+ and K+ were measured by flame spectroscopy with a Solaar spectrometer.
-
bioRxiv - Immunology 2019Quote: ... Cells were blocked overnight at 4°C with 150µl blocking solution per well (5% (v/v) human serum (Sigma), 5% (v/v ...
-
bioRxiv - Molecular Biology 2020Quote: ... Cell disruption was carried out by vortexing (3 cycles, 5 min each) at 4°C using 0.2 ml of glass beads (425-600 μm; Sigma). For the Rpb3 immunopurification ...
-
bioRxiv - Immunology 2020Quote: ... 1 mL of lentiviral supernatant was added to 5×105 RAW 264.7 macrophages with 4 μg/mL of hexadimethrine bromide (Millipore) and cells were spinoculated at 300 x g for 30 minutes at room temperature ...
-
bioRxiv - Biochemistry 2022Quote: ... 4 mM DTT and 5% D2O at a protein concentration 1 mM using a Shigemi NMR tube (Sigma Aldrich). Data was collected on an ultrahigh field Bruker 900 MHz NMR spectrometer equipped with a triple resonance cryogenic probe ...
-
bioRxiv - Microbiology 2022Quote: ... 4 °C), resuspended in 5 ml lysis buffer (150 mM NaCl, 50 mM Tris-HCl, 0.5ξCellLytic B (Sigma-Aldrich), 1 % Triton X-100 ...
-
bioRxiv - Neuroscience 2023Quote: ... Immunoprecipitation was carried out by rotating sonicated lysates overnight at 4 C with NFIA antibody (5 mg, Sigma, HPA006111) or SOX9 antibody (EMD Millipore ...
-
bioRxiv - Molecular Biology 2022Quote: ... Excess TMT reagent was quenched by the addition of 4 µl of an aqueous 5% hydroxylamine solution (Sigma, 438227). Peptides were reconstituted in 0.1 % formic acid ...
-
bioRxiv - Systems Biology 2022Quote: ... The lysate was centrifuged at 4,000 xg for 5 min at 4°C and the protein pelletwas resuspended in Digestion Buffer(10% 2,2,2-Trifluoroethanol (Sigma; #96924)) in 100 mM HEPEs pH 8.5) ...
-
bioRxiv - Cell Biology 2022Quote: ... Excess TMT reagent was quenched by the addition of 4 μl of an aqueous 5 % hydroxylamine solution (Sigma, 438227). Peptides were reconstituted in 0.1 % formic acid ...
-
bioRxiv - Synthetic Biology 2024Quote: ... washed once with TBS pH 8.0 for 5 min and incubated for overnight at 4 °C in primary antibody (Mouse-anti-FLAG M2 [Sigma #F1804 ...
-
bioRxiv - Microbiology 2023Quote: ... Excess TMT reagent was quenched by the addition of 4 µl of an aqueous 5% hydroxylamine solution (Sigma, 438227). Peptides were reconstituted in 0.1% formic acid and equal volumes were mixed ...
-
bioRxiv - Cell Biology 2023Quote: ... pelleted by centrifugation (5 min, 400 gavg, 4°C) and washed in phosphate buffered saline (PBS, Sigma, D8537-500ml), before being pelleted again ...
-
bioRxiv - Biochemistry 2024Quote: ... Transferred PVDF membranes were blocked in 5% milk powder in 0.2% Tween-TBS overnight at 4°C and immunoblotted with mouse anti-FLAG-HRP (Sigma) conjugated primary antibody ...
-
bioRxiv - Cancer Biology 2024Quote: ... Isolated MLECs were treated with increasing concentration (0.1, 5 and 50 μM) of 4-hydroxytamoxifen (OH-tamoxifen) (Millipore-Sigma) for 24 h ...
-
bioRxiv - Cancer Biology 2024Quote: ... Isolated MLECs were treated with increasing concentration (0.1, 5 and 50 μM) of 4-hydroxytamoxifen (OH-tamoxifen) (Millipore-Sigma) for 24 h ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... and then incubated overnight at 4°C with primary antibodies diluted in blocking solution [5% Goat Serum (Sigma Aldrich) + 0.2% Triton X-100 (Sigma Aldrich ...
-
bioRxiv - Cell Biology 2023Quote: ... Excess TMT reagent was quenched by the addition of 4 µl of an aqueous 5% hydroxylamine solution (Sigma, #438227). Peptides were reconstituted in 0.1 % formic acid and mixed to achieve a 1:1 ratio across all TMT-channels ...
-
bioRxiv - Cell Biology 2023Quote: ... Excess TMT reagent was quenched by the addition of 4 µl of an aqueous 5% hydroxylamine solution (Sigma, 438227). Peptides were reconstituted in 0.1 % formic acid and equal volumes were mixed ...
-
bioRxiv - Molecular Biology 2023Quote: ... The lysate was cleared by centrifugation at 16,000 g for 5 min at 4 °C before it was immobilized on anti-FLAG (Sigma) agarose bead slurry for 30 min at 4 °C ...
-
bioRxiv - Immunology 2023Quote: ... and digested in 4 mL of RPMI-1640/5% HI-FBS containing 1 mg/mL collagenase D (Sigma-Aldrich) and 30 μg/mL DNase I (Sigma ...
-
bioRxiv - Immunology 2023Quote: Male mice were sensitized with 4 mg of PN butter (∼1 mg of protein; Capitán Mani) and 5 μg of cholera toxin (C8052, Sigma) in 0.5 mL of PBS administered intragastrically ...
-
bioRxiv - Cell Biology 2023Quote: The cell proliferation assay was determined by 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay (Sigma). All cancer cell lines were seeded in 24-well plates (2×104 cells/well) ...
-
bioRxiv - Developmental Biology 2023Quote: Membranes were incubated overnight in primary antibody o/n at 4°C: Rabbit ⍺-Vang (Rawls & Wolff, 2003) diluted 1:1,000 in 5% milk in TBST or Rabbit ⍺-Actin (Sigma) diluted 1:2,000 in 10% Seablock in TBST followed by 3x wash in TBST for 10 minutes each ...
-
bioRxiv - Genomics 2023Quote: Undifferentiated shCTRL and shCPOX-4 Cells seeded at 0.5M cells/ml and treated with 5 μl 10mM GSK343 (Sigma, catalog no ...
-
bioRxiv - Cell Biology 2023Quote: ... gel samples were prepared by boiling for 5 min in 2x SDS sample buffer (125 mM Tris, 4% SDS, 20% glycerol, 0.04% bromophenol blue, pH 6.78) supplemented with 5% β-mercaptoethanol (Sigma). Samples were briefly centrifuged prior to loading onto 4-12% NuPAGE Bis-Tris mini-gels (ThermoFisher Scientific) ...
-
bioRxiv - Cancer Biology 2024Quote: ... Beads were re-suspended in antibody buffer (50 μL) with BG4 (∼5 μM, 4 μL/sample) or H3K27ac antibody (0.5 μL/sample, Millipore) and incubated at 4 ◦C overnight ...
-
bioRxiv - Neuroscience 2024Quote: ... 5 minutes each) prior to incubating in blocking solution (4% BSA in PBS with 0.33% Tween-20, Sigma-Aldrich) at room temperature for 30 minutes ...
-
bioRxiv - Cancer Biology 2024Quote: ... Nuclei pellets were then collected by centrifuge for 4 min at 1,300 × g at 4°C and washed once in PBS supplemented with 5 mM sodium butyrate (Sigma) before binding to prepared concanavalin A coated magnetic beads (Bangs Laboratories ...
-
bioRxiv - Physiology 2024Quote: ... WT and Gw females (4 months of age) underwent superovulation by intraperitoneal (IP) injection of 5 IU pregnant mare’s serum gonadotrophin (PMSG; Sigma Cat.# G4877-1000 IU ...
-
bioRxiv - Developmental Biology 2024Quote: ... at 4 x 105 cells/well on 24-well plate (Trueline) coated with 5 μg/ml fibronectin (Sigma-Aldrich). Cells were treated with either 0.01% DMSO or different concentrations of Ki8751 inhibitor for 6 hours ...