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Citations for Millipore Sigma :
3451 - 3500 of 10000+ citations for Recombinant Human IL18 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... The proteins were then transferred onto a nitrocellulose membrane (Millipore). The membranes were blocked using Odyssey Blocking Buffer (LI-COR ...
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bioRxiv - Microbiology 2020Quote: ... lysed in BugBuster Primary Amine Free Protein Extraction Reagent (Novagen) supplemented with recombinant lysozyme ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein complexes were eluted with 3xFlag peptide (200ug/mL, Sigma) in 5 column volumes Buffer 2 for 1 hour at 4C ...
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bioRxiv - Systems Biology 2020Quote: ... proteins were transferred to Immobilon FL PVDF membrane (Merck-Millipore) using semi-dry transfer (25 V for 30 min.) ...
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bioRxiv - Biophysics 2019Quote: ... then bound proteins were eluted with 3 mM desthiobiotin (Sigma) or 50 mM D-biotin (CHEM-IMPEX ...
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bioRxiv - Cell Biology 2019Quote: ... protein inhibitor and phosphatase inhibitor (Complete-EDTA free, Roche/Sigma prod ...
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bioRxiv - Developmental Biology 2019Quote: ... rabbit anti-Cytoplasmic interacting protein 1 (Millipore-Sigma, Cat # Ab6046), rabbit anti-β-Catenin polyclonal antibody (ThermoFisher ...
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bioRxiv - Developmental Biology 2019Quote: ... rabbit anti-Cytoplasmic interacting protein 1 (Millipore-Sigma, Cat # Ab6046), rabbit anti-β-Catenin polyclonal antibody (ThermoFisher ...
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bioRxiv - Microbiology 2019Quote: ... 20 µL protein A agarose suspension (Calbiochem/Millipore #IP02-1.5ML) was added to each and samples were rocked at 4°C for 2 hours ...
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bioRxiv - Cancer Biology 2019Quote: ... The proteins were then transferred on PVDF membrane (Millipore, USA) by overnight wet transfer at 30 volts ...
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bioRxiv - Microbiology 2020Quote: ... Protein quantification was performed using the Bradford Reagent (Sigma-Aldrich). Equal amounts of protein lysates were separated by SDS-PAGE and proteins were transferred to nitrocellulose filters ...
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bioRxiv - Cell Biology 2019Quote: ... Relative protein loading was visualized using Ponceau S Solution (Sigma). Membranes were subsequently washed in TBST and blocked for 1 hour in 5% skim milk in TBST at RT ...
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bioRxiv - Systems Biology 2019Quote: ... proteins were reduced by adding 10 mM DTT (Sigma Aldrich) prior to alkylation with 55 mM iodoacetamide (Sigma Aldrich) ...
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bioRxiv - Molecular Biology 2019Quote: ... The proteins were transferred onto a polyvinylidene fluoride membrane (Millipore). The membrane was blocked with 3% BSA/Tris-buffered saline that contained 0.1% Triton X-100 (TBST ...
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bioRxiv - Genetics 2019Quote: ... conjugated on Protein A+G Magnetic beads (Millipore, 16-663). Immunoprecipitated DNA was amplified by PCR and subjected to Illumina sequencing.
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bioRxiv - Zoology 2019Quote: ... Hsp70 protein was labelled with anti-HSP70 (Sigma-Aldrich, # H9776) at 1:3000 ...
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bioRxiv - Immunology 2019Quote: ... and the antibody was purified with Protein G (Sigma-Aldrich). Mice were injected every 2 days for a total of 9 injections with 1.5 μg of the anti-c-Fms antibody in 3 μL of phosphate-buffered saline (PBS) ...
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bioRxiv - Physiology 2019Quote: ... followed by determining protein concentration using BCA assay kit (SIGMA). Samples were loaded onto SDS-PAGE for protein expression analysis.
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bioRxiv - Molecular Biology 2020Quote: 20 μg protein was incubated in 5x Laemmli buffer (Sigma) at 95°C for 10 min and separated by a Mini-PROTEAN® TGX (Bio-Rad ...
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bioRxiv - Cell Biology 2019Quote: ... The proteins were transferred to PVDF membranes (Immobilon-P, Millipore) and probed with antibodies to detect the FLAG fusions and endogenous proteins ...
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bioRxiv - Biochemistry 2019Quote: ... Protein concentrations were determined using the Bradford assay (Sigma-Aldrich) with bovine serum albumin as standard.
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bioRxiv - Systems Biology 2019Quote: ... Separated protein lysates were transferred to PVDF membranes (Millipore #IPVH00010), which were subsequently rinsed in TBST (Tris-buffered saline ...
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bioRxiv - Genetics 2019Quote: ... Proteins were transferred from gels to Immobilon-P membrane (Millipore) and probed with antibodies as described 20 ...
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The conserved metalloprotease invadolysin is present in invertebrate haemolymph and vertebrate bloodbioRxiv - Cell Biology 2019Quote: ... and protein was quantified using a Bradford assay kit (Sigma) following manufacturers’ instructions.
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bioRxiv - Neuroscience 2019Quote: ... Proteins were detected with antibodies against GFP (Sigma, 1:1000) and FMR polyclonal antibodies (1:1000) ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were transferred to 0.45 μm PVDF membranes (Merck Millipore), and blocked with 5% skim milk in TBS-0.1% Tween20 (TBST ...
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bioRxiv - Cell Biology 2019Quote: ... Protein concentration was determined using the Direct Detect assay (Millipore). Protein samples were run on 4–15% Mini-PROTEAN TGX gels (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: Proteins were extracted using RIPA buffer (Sigma-Aldrich, 20-188) supplemented with Complete Mini EDTA-free Protease inhibitor cocktail (Roche ...
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bioRxiv - Biochemistry 2019Quote: ... and supplemented with SyproOrange Protein Gel Stain 1:500 (Sigma). After incubation on ice for 1.5 h ...
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bioRxiv - Biochemistry 2019Quote: ... The transferred proteins were visualized with chemiluminescence detection kit (Millipore).
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bioRxiv - Biochemistry 2019Quote: ... Proteins were transferred onto PVDF membranes (Millipore, Burlington, MA, USA). The blots were blocked for 2 h at room temperature with 5% non-fat milk in PBS/0.05% Tween 20 and then incubated with the primary antibody (1 µg/mL ...
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bioRxiv - Physiology 2019Quote: ... Protein transfer was assessed by ponceau red staining (Sigma-Aldrich). Membranes were blocked with 5% bovine serum albumin (BSA ...
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bioRxiv - Physiology 2019Quote: ... Eluted proteins were run through Amicon Ultracel 10K (Millipore, #UFC501096) to concentrate in a final volume of 50μL ...
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bioRxiv - Plant Biology 2021Quote: ... and protein was precipitated by 10% TCA (trichloroacetic acid, Sigma) in acetone ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Protein concentration was determined using Bradford reagent (B6916, Sigma-Aldrich). For offline high pH reversed-phase (hpH RP ...
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bioRxiv - Biophysics 2021Quote: ... Purified protein was concentrated using Amicon Ultra-Centrifugal units (Millipore) and flash frozen for storage at −80°C or used immediately for experiments.
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bioRxiv - Immunology 2021Quote: ... The proteins were concentrated with Amicon (cutoff 10 kDa, Sigma) to achieve around 0.200 mg/mL of protein solution for mass spectrometry.
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bioRxiv - Microbiology 2021Quote: ... the protein solution was reduced with 10 mM DTT (Sigma) for 1 h at 37 °C and alkylated with 20 mM IAA(Sigma ...
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bioRxiv - Evolutionary Biology 2020Quote: ... proteins were transferred to Immobile FL PVDF membrane (EMD Millipore) and immunoblotting was performed with anti-FLAG M2 (F1804 ...
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bioRxiv - Cancer Biology 2021Quote: ... the proteins were visualized with Chemiluminescent HRP Substrate (Merk Millipore) and signals recorded using Bio-Rad ChemiDoc MP chemiluminescence imaging system (BIORAD).
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bioRxiv - Cell Biology 2021Quote: ... The proteins were concentrated with Amicon Ultra-15 filters (Millipore) with molecular weight cutoffs of 30 kDa and 50 kDa for ICR1-His6 and ICR2-His6 ...
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bioRxiv - Biochemistry 2021Quote: ... the proteins were incubated with SYPRO orange dye (Sigma-Aldrich), which binds specifically to hydrophobic amino acids leading to an increased fluorescence at 610 nm when excited with a wavelength of 490 nm ...
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bioRxiv - Biochemistry 2021Quote: ... Protein A/G agarose beads (EMD Millipore, cat. no. IP05) were incubated with primary antibody for 2 hours at 4°C before addition to cell lysate ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The total proteins were transferred to PVDF membrane (Millipore, USA). Transferred protein quantities were detected with Ponseau S solution (Sigma-Aldrich ...
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bioRxiv - Biophysics 2021Quote: ... Eluted protein was crosslinked with 0.04% glutaraldehyde (EM-grade, Sigma) on ice for 25 min ...
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bioRxiv - Biophysics 2021Quote: SSB protein from Escherichia coli was purchased from Sigma-Aldrich/Merck ...
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bioRxiv - Cell Biology 2021Quote: ... carrier protein and 0.2 mL of 100% Trichloroacetic acid (Sigma) was added to the extracted protein fraction ...
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bioRxiv - Cell Biology 2021Quote: ... and protein concentration was determined with Bradford reagent (Sigma-Aldrich). For further analysis ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by incubation with Protein A Agarose beads (Sigma-Aldrich) for 1 h at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein were transferred to Immobilon-P PVDF membrane (IPVH00010, Millipore) either for 1 hour at 100V at 4°C or overnight at 35V and 4°C ...