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Citations for Millipore Sigma :
3701 - 3750 of 10000+ citations for Recombinant Human IL18 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentration was determined using Bradford reagent (Sigma-Aldrich, USA) on a spectrophotometer (Infinite F50 Tecan ...
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bioRxiv - Cancer Biology 2024Quote: ... Secreted proteins were reduced in 10mM Dithiothreitol (DTT; Sigma-Aldrich) at 20°C for 1 hour ...
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bioRxiv - Cancer Biology 2024Quote: ... GST protein was purified using glutathione Sepharose 4B beads (Sigma) by rotating overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were transferred to a 0.2 μm PVDF membrane (Millipore) and probed with primary antibodies overnight at 4°C ...
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bioRxiv - Systems Biology 2024Quote: ... conjugated to protein G agarose beads (Sigma-Aldrich, catalog # IP04). For IP of phosphopeptides containing the (pS/pT)Q and (pS/pT)P phospho-motifs ...
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bioRxiv - Molecular Biology 2024Quote: ... 40 μl of prewashed protein A beads per sample (Sigma; prewashed in lysis buffer as mentioned in the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein solutions were concentrated using Amicon Ultra centrifugal filter (Millipore) for application to FPLC AKTA pure (GE Heathcare) ...
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bioRxiv - Physiology 2024Quote: Protein concentrations were measured using the Bradford reagent (Sigma #B6916). Samples were added at a 1:200 ratio in a 96-well plate and incubated at room temperature for 5 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... then after preclearing with protein A–Sepharose beads (Sigma-Aldrich), HY5 antibody (AS12 1867 ...
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bioRxiv - Neuroscience 2024Quote: ... 2015 using a compartment protein extraction kit (Millipore cat: 2145). Triturated brain tissue was thawed on ice for ∼30 minutes before being homogenized in 2 ml/g of Buffer C (HEPES (pH7.9) ...
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bioRxiv - Neuroscience 2024Quote: ... Protein was transferred to an Immobilon PVDF membranes (Millipore Sigma) via wet transfer at 30V for 1 hour at 4 °C in 10% Methanol Tris/Glycine buffer using a Criterion™ Blotter (BioRad) ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were then transferred to PVDF membranes (Millipore, Cat# IPVH00010) at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... proteins were transferred to Immobilon-FL PVDF membranes (Merck-Millipore) and washed twice in TBS buffer followed by incubation in Intercept-TBS Blocking Buffer (Li-COR ...
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bioRxiv - Genetics 2024Quote: ... Protein bands were visualized using enhanced chemiluminescence reagents (Millipore, WBKLS0500).
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bioRxiv - Immunology 2024Quote: ... wells were coated with Protein A from Staphylococcus aureus (Sigma). After 48h ...
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bioRxiv - Cancer Biology 2024Quote: ... and subsequently with protein A agarose beads (16-125, Millipore) for another 4 h at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein samples (50 µg) were reduced with DTT (Millipore Sigma) at a final concentration of 20 mM at 95°C for 10 minutes and alkylated with iodoacetamide (Millipore Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... and the protein amounts were determined by BCA assay (Sigma). The proteins were denatured by boiling for 10 min in the DTT-containing sample buffer and separated by 4–20% Mini-PROTEIN TGX gels (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Total protein was visualized using Ponceau S stain (Sigma-Aldrich).
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bioRxiv - Immunology 2023Quote: ... Bound proteins were reduced with 5 mM DTT (Sigma-Aldrich) in 50 mM TEAB (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were concentrated using Amicon Ultra Centrifugal Filters (Millipore).
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bioRxiv - Genomics 2023Quote: ... per 20 μl of protein A/G MagnaBeads (Merck Millipore). Beads were then washed once with low salt wash buffer (0.1% SDS ...
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bioRxiv - Neuroscience 2022Quote: Protein concentrations were determined using the Bradford method (B6916, Sigma), samples were boiled for 5 min in 4x-Laemmli’s sample buffer (1610747 ...
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bioRxiv - Developmental Biology 2022Quote: ... rabbit anti-pro-surfactant protein C (Sigma, Ab3786; 1:2000), rabbit anti-TGFβ2 (Proteintech ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were transferred to a PVDF-FL membrane (05317, Millipore) with a current of 2.5 mA/cm2 for 1 h ...
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bioRxiv - Microbiology 2023Quote: Total protein concentration was determined colorimetrically (Bradford Reagent, Sigma-Aldrich). The standard curve was prepared using Bovine Serum Albumin (Promega) ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were transferred at 100 V to PVDF membrane (Millipore) for 2 hours ...
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bioRxiv - Cell Biology 2023Quote: ... Eluted protein was concentrated using a centrifugal filter (Millipore; UFC803024) in storage buffer (20 mM Tris HCl ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were transferred to PVDF membranes (EMD Millipore, Darmstadt, Germany), blocked with 5% bovine serum albumin in TBST (20 mM Tris pH 7.4 ...
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bioRxiv - Molecular Biology 2023Quote: ... protein samples were transferred to a Immobilon NC membrane (Millipore) using a Genie Blotter (Idea Scientific) ...
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bioRxiv - Physiology 2023Quote: ... and protein concentration was measured using the BCA Assay (Sigma).
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bioRxiv - Biophysics 2023Quote: ... Proteins from purchased powder-stocks were equine myoglobin (Sigma-Aldrich), bovine ribonuclease A (RNaseA ...
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bioRxiv - Physiology 2023Quote: ... and protein transfer was performed using a PVDF membrane (Millipore). Blots were blocked with LI-COR PBS-blocking buffer and probed with the following primary antibodies at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were eluted with 10 mg/ml reduced glutathione (Sigma) in 50mM Tris pH 8 ...
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bioRxiv - Developmental Biology 2023Quote: ... Proteins were transferred to Immobilon-P membrane (Merck Millipore Ltd.) and westerns preformed with Ift140 (23 ...
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bioRxiv - Cell Biology 2023Quote: ... All the eluted proteins were concentrated by centrifugal filtrations (Millipore), loaded onto desalting columns (GE Healthcare) ...
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bioRxiv - Neuroscience 2023Quote: ... immunoprecipitated proteins were eluted with 3X FLAG peptide (Sigma F4799). Trem2 was immunoprecipitated with either a Rabbit monoclonal antibody raised against the carboxy-terminus of mouse Trem2 (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentrations were measured using BCA assay (Sigma-Aldrich #1003290033). 40–50 μg of cell lysate were loaded in precast SDS-PAGE (sodium dodecyl sulfate–polyacrylamide gel electrophoresis ...
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bioRxiv - Cell Biology 2023Quote: ... the proteins were transferred to a PVDF membrane (Immobilon, Millipore), which was subsequently blocked for 1 h at room temperature (5% Marvel milk (Premier foods ...
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The membrane-cytoplasmic linker defines activity of FtsH proteases in Pseudomonas aeruginosa clone CbioRxiv - Biochemistry 2023Quote: ... Proteins in gels were stained with SYPRO orange (Sigma-Aldrich), and gels were imaged with a Typhoon FLA9500 scanner (GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... Protein loading was visualized with Amido Black Staining Solution (Sigma).
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bioRxiv - Evolutionary Biology 2023Quote: ... Protein expression was induced by adding L-arabinose (Sigma-Aldrich) to a final concentration of 0.2% when the cultures reached a OD600 of 0.4 ...
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bioRxiv - Cancer Biology 2023Quote: Protein was isolated using RIPA buffer with protease (Sigma Aldrich) and phosphatase cocktail inhibitors (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... Protein concentration was determined using Bradford reagent (Sigma Aldrich, USA) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were precipitated by adding 5-sulfosalicylic acid (Sigma-Aldrich) to a final concentration of 1% ...
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bioRxiv - Cell Biology 2023Quote: ... and proteins were transferred to polyvinylidene difluoride membranes (Millipore, IPVH00010). Membranes were blocked in 5% skim milk (Nacalai ...
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bioRxiv - Biochemistry 2023Quote: ... Activated protein was transferred to a PVDF membrane (ED Millipore) using the Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad Laboratories) ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were transferred onto a Polyvinylidene fluoride (PVDF) membrane (Millipore) and blocked with 5% nonfat dry milk in TBST (20 mM Tris-HCl ...
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Targeting adipocyte ESRRA promotes osteogenesis and vascular formation in adipocyte-rich bone marrowbioRxiv - Molecular Biology 2023Quote: ... proteins were transferred onto a PVDF membrane (Merck Millipore #IPFL00010) and blocked with 5% skimmed milk-PBST ...
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bioRxiv - Microbiology 2023Quote: ... Reduced proteins were alkylated with 55 mM Iodoacetamide (Sigma-Aldrich) for 45 min at 37°C in the dark followed by the same washing-drying step described above ...