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Citations for Millipore Sigma :
301 - 350 of 7557 citations for ssc mir 376a 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... colonies were stained with the Alkaline Phosphatase Detection Kit (Sigma-Aldrich) according to manufacturer’s instructions.
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bioRxiv - Pathology 2021Quote: Detection of ROCK activity was performed with a kit from Millipore. Briefly ...
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bioRxiv - Cell Biology 2022Quote: ... and bands were detected using chemiluminescence detection kit (Merck Millipore; WBKLS0050).
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bioRxiv - Cell Biology 2023Quote: ... and bands were detected using chemiluminescence detection kit (Merck Millipore; WBKLS0050).
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bioRxiv - Cancer Biology 2023Quote: ... ab13420).TUNEL staining was performed with an Apoptosis Detection Kit (Millipore, S7100). Images were visualized with the EVOS® FL Auto Imaging System (AMC1000 ...
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bioRxiv - Microbiology 2023Quote: ... Metabolites extracts were then used in Coenzyme A detection kit (Sigma) to quantify cellular CoA.
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bioRxiv - Developmental Biology 2024Quote: ... TUNEL (In Situ Cell Death Detection Kit, TMR Red, Sigma/Roche); anti-Kmg (Fuller lab).
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bioRxiv - Cancer Biology 2021Quote: Telomerase activity was confirmed using the TRAPeze® RT kit (Sigma) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Telomerase activity was confirmed using the TRAPeze® RT kit (Sigma) (13–16) ...
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bioRxiv - Developmental Biology 2019Quote: ... Each real-time qPCR reaction mix was prepared with SYBR Green JumpStart Taq ReadyMix (Sigma, Oakville, ON, Canada) and forward and reverse primer mix (3 µM ...
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bioRxiv - Cancer Biology 2023Quote: ... Hsa-miR-203 mimics were purchased from Sigma Aldrich (MISSION microRNA mimics) and transient transfection was performed using Lipofectamine 2000 (Sigma) ...
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bioRxiv - Cell Biology 2022Quote: ... the slides were washed 3 times for 5 mins while gently shaking at RT with PBS + 0.1% Tween (Sigma) and stained for 90 mins in blocking buffer with a combination of secondary antibodies ...
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bioRxiv - Immunology 2023Quote: ... and secondary antibodies were diluted in IF buffer and incubated for 1 hour at RT and washed 3 more times with PBS 0.05% Tween20 (Sigma). Sections were incubated with PBS DAPI (5 µg/mL ...
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bioRxiv - Cancer Biology 2021Quote: RIP assays were conducted to explore the relationship between lncRNA NEAT1 and miR-1299 via the Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore, MA, USA). Initially ...
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bioRxiv - Cell Biology 2021Quote: ... RT-qPCR analysis was performed using the KAPA SYBR Fast PCR master mix (Sigma Aldrich, #KK4605) or SYBR Green PCR Master Mix (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... RT-PCR on both gDNA and cDNA was performed using the SYBR® Green JumpStart (Sigma) in a 7500 Fast Real Time PCR cycler from Applied Biosystems.
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bioRxiv - Developmental Biology 2021Quote: TUNEL analysis was performed using the In Situ Cell Death Detection Kit (Kit #11684795910, Sigma Aldrich) following manufacturer’s recommendations and fluorescein-dUTP ...
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bioRxiv - Cancer Biology 2020Quote: ... instructions provided by the kit (Annexin V-FITC Apoptosis Detection Kit, Sigma, Catalog No. APOAF-50TST) was followed ...
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bioRxiv - Microbiology 2022Quote: LDH was measured using an LDH Cytotoxicity Kit (Pierce, 88953) or Cytotoxicity Detection Kit (Sigma, 11644793001) per the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... The HybriSlips were removed by dipping the slides in 2x SSC (Sigma Aldrich, S6639)/0.1% Tween20 ...
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bioRxiv - Biophysics 2023Quote: ... After a washing with 2x Saline-Sodium Citrate buffer (SSC, Sigma-Aldrich, cat. S6639), coverslips were incubated overnight at RT in a solution of 50% formamide (pH 7.0 ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by 3 min at 60 in 0.4× SSC/0.3 % IGEPAL (Sigma-Aldrich Inc.) and further 3 min in 2× SSC/0.1 % IGEPAL at RT ...
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bioRxiv - Genetics 2023Quote: ... chromosome denaturation was done in 75% formamide in 2× SSC (pH 7.0) (Sigma-Aldrich) at 72 °C ...
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bioRxiv - Cell Biology 2023Quote: We then rinsed coverslips in 2xSSC (20x SSC, Sigma, #S6639 diluted in ultrapure H2O), then permeabilized cells in a solution of 0.2 M HCl (Sigma ...
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bioRxiv - Genomics 2024Quote: ... cells were incubated with hybridization buffer (15% formamide from Sigma-Aldrich in 1× SSC) for 15 min ...
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bioRxiv - Cell Biology 2020Quote: Intracellular NO was measured in real time using the NO-specific fluorescence probe DAF-FM DA solution (Sigma Aldrich). DAF-FM DA diffuses freely across the membrane ...
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bioRxiv - Cell Biology 2020Quote: ... and signals were visualized with Visualizer™ Western Blot Detection Kit (Millipore). Band analysis was performed using ImageLab software from BioRAD and normalized to cell counts.
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bioRxiv - Developmental Biology 2021Quote: ... alkaline phosphatase staining was performed using the Alkaline Phosphatase Detection Kit (Millipore). Colonies were counted manually ...
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bioRxiv - Microbiology 2019Quote: ... infection using the VenorGEM Mycoplasma detection kit (Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Bioengineering 2021Quote: ... the protein bands were developed using an enhanced chemiluminescent detection kit (Millipore). The optical bands were visualized in a Fuji Las-3000 dark box (FujiFilm) ...
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bioRxiv - Cancer Biology 2020Quote: ... the ApopTag® Red In Situ Apoptosis Detection Kit (S7165, Merck Millipore) was also used to label apoptotic cells following Wells and Johnston protocol (Wells and Johnston ...
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bioRxiv - Cancer Biology 2019Quote: Cell viability was measured using the CCK-8 detection kit (Sigma-Aldrich) according to the protocol of manufacturer.
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bioRxiv - Developmental Biology 2019Quote: ... we used the In Situ Cell Death Detection Kit (Sigma-Aldrich, 11684795910), following the manufacturer’s protocol (ver ...
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bioRxiv - Neuroscience 2020Quote: ... brain sections were treated with in situ Cell Death Detection Kit (Sigma).
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bioRxiv - Bioengineering 2021Quote: ... the protein bands were developed using an enhanced chemiluminescent detection kit (Millipore). The optical bands were visualized in a Fuji Las-3000 dark box (FujiFilm) ...
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bioRxiv - Cell Biology 2020Quote: ... and Magic Red Cathepsin B Detection Assay Kit (CS0370) were from Sigma. siR-Lysosome (CY-SC016 ...
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bioRxiv - Genetics 2022Quote: ... The western blotting signals were visualized using an ECL Detection Kit (Millipore). The actin protein served as the protein loading control.
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bioRxiv - Pathology 2023Quote: ... using a commercially available In Situ Cell Death Detection kit (Sigma-Aldrich), according to the manufacturer’s protocol (Fig ...
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bioRxiv - Immunology 2022Quote: Cell viability was assessed using an LDH cytotoxicity detection kit (Sigma Aldrich) per the manufacturer protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... PLA was performed using Duolink in situ detection kit (Sigma-Aldrich, DUO92008) with Duolink PLUS anti-rabbit (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... Percentage cell death was measured using Cell Cytotoxicity Detection kit (LDH; Sigma) as per manufacturer’s instructions.
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bioRxiv - Developmental Biology 2024Quote: ... Using ApopTag Red In Situ Apoptosis Detection Kit (CAT S7165; Sigma-Aldrich), 5 animals per tube were incubated with TdT enzyme mix for 4 hr at 37°C ...
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bioRxiv - Plant Biology 2019Quote: ... membranes were washed with TBST five times for 5 min each and visualized using a chemiluminescence detection system (Immobilon Western HRP Substrate, Merck Millipore). All steps were carried out with phosphate-buffered saline (PBS) ...
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bioRxiv - Developmental Biology 2020Quote: ... membranes were washed 3 times with TBS-T and subjected to chemiluminescence detection with Immobilon Western Chemiluminescent HRP (Horseradish Peroxidase) Substrate (Merck Millipore) using a Gel-Doc XR+ System (BioRad) ...
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bioRxiv - Neuroscience 2020Quote: ... After 2 h at RT plates were washed three times and incubated with anti-human Fab specific-HRP conjugated secondary antibody (Sigma) for 1 h at RT ...
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bioRxiv - Microbiology 2020Quote: ... presence of enzyme inhibitors in RNA extracts was assessed by reverse transcription PCR (eAMV RT, Sigma-Aldrich) using universal primers for 18S RNA ...
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bioRxiv - Cancer Biology 2021Quote: ... Standard RT-PCR using independent sets of Predesigned KiCqStart® SYBR® Green Primers (Sigma, Table S11) was carried out for validation with independent biological replicates from 2D cultures (n = 2 ...
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bioRxiv - Molecular Biology 2023Quote: ... TUNEL staining was performed using a commercial kit (In Situ Cell Death Detection Kit, TMR (Sigma, 12156792910)) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: ... All PCR products were purified with GenElute PCR Clean-Up Kit (Sigma) and sent for Sanger sequencing (Microsynth ...
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bioRxiv - Molecular Biology 2021Quote: AC16 human cardiomyocytes were purchased from EMD Millipore (SSC #109) and cultured in Dulbecco’s modified Eagle’s medium: Nutrient mixture F-12 (Sigma #D6434) supplemented with 2mM L-glutamine ...