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Citations for Millipore Sigma :
551 - 600 of 7557 citations for ssc mir 376a 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2020Quote: ... 2x SSC (300 mM NaCl, 30 mM sodium citrate, pH 7.0)) and 50 μg/mL of salmon sperm DNA (Sigma). FISH was carried out following standard procedures ...
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bioRxiv - Neuroscience 2019Quote: ... samples were pretreated with hybridization buffer (50% formaldehyde, 5 × SSC, 0.1% tween, 100 ug/ml tRNA [Roche], 50 ug/ml heparin [Sigma]) for 2 hr at 65°C ...
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bioRxiv - Genomics 2019Quote: ... The supernatant was discarded and the pellet was resuspended in a small volume of SSC cocktail (3xSSC [Sigma; S0902], 0.04% BSA, 40mM DTT [Sigma; 43816] ...
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bioRxiv - Molecular Biology 2023Quote: ... and added to an additional 50 µL of Buffer H (4x SSC buffer, 4 mg/mL acetylated BSA [Sigma, B8894] ...
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bioRxiv - Developmental Biology 2023Quote: ... the samples were pre-hybridized in hybridization buffer (50% formamide containing 5x SSC, 0.1% Tween-20, 5 mg/mL torula RNA (SIGMA), and 100 µg/mL heparin sulfate ...
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bioRxiv - Developmental Biology 2024Quote: ... organoids were washed twice for 5 minutes with 2 mL of SSCT (0.1% Tween20 in 5x SSC buffer, Sigma) at room temperature.
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bioRxiv - Cell Biology 2022Quote: ... 400nM RT primers (Sigma Aldrich)) and scRNA-seq primers ...
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bioRxiv - Neuroscience 2019Quote: ... Mice were genotyped by PCR on tail DNA using REDExtract-N-Amp Tissue PCR kit (Sigma-Aldrich) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were purified using the GenElute PCR Clean-up kit (Sigma Aldrich, St. Louis, MO, USA) according to instruction manual ...
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bioRxiv - Microbiology 2024Quote: ... PCR products were purified with the GenElute PCR Clean-up kit (Sigma Aldrich, St. Louis, MO, USA) as per instruction manual and triplicates were pooled and diluted 1:5 for a second PCR with Illumina adapter sequences ...
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bioRxiv - Biochemistry 2020Quote: ... PCR stages used the KOD HotStart Polymerase Kit (Sigma-Aldrich); In-gel DNA extraction steps used the E.Z.N.A gel extraction kit (omega BIO-TEK) ...
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bioRxiv - Synthetic Biology 2020Quote: ... PCR products were purified using the GenElute kit (Sigma Aldrich) and concentrations were checked using a NanoDrop ND1000 (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2021Quote: ... from the Kap2G Robust PCR kit (Sigma-Aldrich, Gillingham, UK) and 9 µL added to each well in a 96-well plate ...
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bioRxiv - Neuroscience 2023Quote: ... and cDNA purification (Genelute PCR clean-up kit, Sigma-Aldrich) were carried out ...
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bioRxiv - Microbiology 2023Quote: ... purified with the GenElute PCR Clean-up kit (Sigma Aldrich) again ...
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bioRxiv - Microbiology 2023Quote: ... and purified using the GenElute PCR cleanup kit (Sigma Aldrich). The DNA library was prepared using the Illumina DNA M preparation kit and sequenced on the Illumina NovaSeq 6000 platform at the AGRF ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatant of the lysis after centrifugation was collected and assayed using ALP detection kit (Sigma-Aldrich) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The DNA fragmentation assay used the Cell Death Detection ELISA kit (initially from Roche, later Millipore-Sigma). Phosphatidylserine was detected by FITC-conjugated annexin V (Sigma) ...
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bioRxiv - Microbiology 2020Quote: ... The DNA fragmentation assay used the Cell Death Detection ELISA kit (initially from Roche, later Millipore-Sigma). Phosphatidylserine was detected by FITC-conjugated annexin V (Sigma) ...
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bioRxiv - Molecular Biology 2021Quote: PLA was performed using DuoLink red detection kit and DuoLink anti-mouse and anti-rabbit antibodies (Millipore-Sigma Cat ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were routinely tested for mycoplasma per the manufacturer’s instructions (VenorGeM Mycoplasma Detection Kit; Sigma-Aldrich #MP0025).
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bioRxiv - Molecular Biology 2020Quote: TRAP assays were performed following the manufacturer’s protocol for the TRAPeze Telomerase Detection Kit (EMD-Millipore S7700). Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... and penicillin/streptomycin) and routinely tested for mycoplasma using the Venor GeM Mycoplasma Detection Kit (Sigma-Aldrich). For MPI knockdown experiments ...
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bioRxiv - Immunology 2021Quote: ... for flow cytometry and confocal microscopy and by using anti-modified citrulline detection kit (clone C4; Millipore) for immunoblot according to manufacturer’s protocol.
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bioRxiv - Molecular Biology 2019Quote: Apoptosis was determined using the Annexin V-PI or Annexin V-7AAD Apoptosis Detection Kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Cultured cells and parasites were regularly screened against mycoplasma contamination using the LookOut Mycoplasma detection kit (Sigma) and cured with Mycoplasma Removal Agent (Bio-Rad ...
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bioRxiv - Cancer Biology 2019Quote: ... TUNEL staining for apoptosis was performed using the Apoptag Fluorescein in situ Apoptosis Detection Kit (Millipore; S7110) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Interactions between CB1 and cytoskeleton proteins were detected using the Duolink in situ PLA detection Kit (Sigma) following the instructions of the supplier ...
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bioRxiv - Immunology 2021Quote: Proximity ligation assays were performed using the Duolink® flowPLA Detection Kit – Orange (Millipore Sigma, Cat. DUO94003) according to manufacturers recommended protocol with minor changes ...
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bioRxiv - Immunology 2020Quote: ... The TUNEL assay was performed using the ApopTag Plus Peroxidase In Situ Apoptosis Detection Kit (Merck Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... and then treated with ligase from the Duolink In Situ Detection Reagents Green kit (Sigma, Cat# DUO92014) for 30 minutes in a humidity chamber at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Using the commercial Click-iT TUNEL (In Situ Cell Death Detection Kit, Fluorescein – Sigma-Aldrich – ref 11684795910) colorimetric detection kit ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell lines were routinely tested for Mycoplasma contamination using the Venor GeM Mycoplasma Detection Kit (Sigma, MP0025).
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bioRxiv - Cell Biology 2023Quote: Proximity ligation assay experiments were carried out using Duolink® flowPLA Detection Kit - FarRed (Sigma Aldrich, # DUO94004) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... All cell lines were routinely screened for the presence of mycoplasma (Mycoplasma detection kit, Sigma-Aldrich, MP0035).
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bioRxiv - Developmental Biology 2023Quote: ... Apoptosis was determined by performing TUNEL analysis using the ApopTag Red In Situ Apoptosis Detection Kit (Millipore). The total number of TUNEL positive cells were quantified per high powered field (HPF ...
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bioRxiv - Biophysics 2023Quote: ... TUNEL staining was performed using an ApopTag Fluorescein in situ apoptosis detection kit (S7110, EMD Millipore, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: SaβG staining of aortas for senescent cells was examined using a Senescence detection assay kit (Millipore-Sigma Cat No.KAA002 ...
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bioRxiv - Immunology 2023Quote: ... and routine testing for Mycoplasma contamination was performed using the Venor GeM Mycoplasma Detection Kit (Sigma, MP0025).
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bioRxiv - Biochemistry 2024Quote: ... Cell lines were verified to be mycoplasma-free with the LookOut Mycoplasma qPCR detection kit (Sigma-Aldrich).
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A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... Mycoplasma was monitored in the U937 macrophages using the Venor GeM Mycoplasma Detection Kit (Sigma-Aldrich #MP0025). Two days prior to challenge with L ...
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bioRxiv - Cancer Biology 2024Quote: ... TUNEL analysis was carried out using the ApopTag Peroxidase in situ cell apoptosis detection kit (Millipore, S7100) according to the manufacturer’s directions ...
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bioRxiv - Physiology 2021Quote: ... DNA fragments were directly purified from the PCR mix using the GenElute PCR Clean-Up Kit (Sigma-Aldrich). Gibson assemblies were performed with the NEBuilder HiFI DNA Assembly Master mix (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: ... The PCR products were purified using the GenElute PCR Clean-up kit (Sigma Aldrich, St. Louis, MO, USA) according to instruction manual ...
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bioRxiv - Developmental Biology 2020Quote: ... embryos were resuspended in 100 μL anti-fade buffer (0.4 % glucose, 10 μM Tris-HCl pH8, 2x SSC) with 1 μL catalase (Sigma-Aldrich) and 1 μL glucose oxidase (3.7 mg/mL ...
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bioRxiv - Cell Biology 2022Quote: ... Hybridisation was performed by incubating coverslips with 100 - 250 nM probes diluted in hybridisation buffer (2X SSC buffer, 10% Dextran sulfate (Sigma), 10% Formamide ...
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bioRxiv - Biophysics 2021Quote: ... Thereafter, the cells were washed twice with washing buffer (25% formamide, 2X SSC) containing DAPI (1 mg/mL, Sigma Aldrich), for 30 minutes at 30°C ...
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bioRxiv - Cell Biology 2022Quote: ... Fixed ovaries were washed twice in PBST for 10 minutes and incubated with a final probe concentration of 1nM/probe in 250μL HYBEC solution (2x SSC, 15% ethylene carbonate (Sigma Aldrich), 1mM EDTA ...
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bioRxiv - Molecular Biology 2022Quote: ... 16806-25] in 10 mM Tris-HCl [pH 8.0], 2× saline sodium citrate [SSC], glucose oxidase [37 µg/mL, Sigma-Aldrich, G2133-10KU] ...
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bioRxiv - Neuroscience 2022Quote: ... the sample was washed 4 times with 2X SSC and fluorophores were cleaved off by reducing the thiol bond with 50 mM tris(2-carboxyethyl)phosphine (TCEP, Sigma) in 2X SSC during two washes of 10 minutes at 22 °C ...