Labshake search
Citations for Millipore Sigma :
301 - 350 of 7599 citations for Zika Virus Envelope Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2022Quote: The virus was precipitated from the cell supernatant by adding 8 % (w/v) polyethylene glycol (PEG) 8000 (Sigma-Aldrich), incubating at +4 °C with gentle mixing for 3 h ...
-
bioRxiv - Systems Biology 2023Quote: ... HEK293 cells were then transduced overnight with the filtered virus in the presence of 8 µg/mL polybrene (Millipore); the amount of virus used was optimized to ensure the infection rate of ∼20% ...
-
bioRxiv - Physiology 2024Quote: ... C2C12 mouse myoblasts were seeded 24h prior to infection and then transduced with virus-containing supernatant supplemented with 8μg/mL polybrene (Millipore) as shown previously40 ...
-
bioRxiv - Genetics 2023Quote: ... RPE-1 cells were transduced with lentivirus at an MOI of 0.3-0.5 (usually between 50-100 µL of virus/well) and 10 µg/mL Polybrene (Sigma-Aldrich). After 48 hours ...
-
bioRxiv - Microbiology 2023Quote: ... The medium for the culture of influenza A virus in MDCK cells (virus medium) was prepared by supplementing Minimal Essential Medium with 4.3% bovine serum albumin (BSA; Sigma) and Normocin.
-
bioRxiv - Microbiology 2024Quote: ... The virus particles were further purified by layering them over a discontinuous 5% to 50% Nycodenz gradient (Sigma-Aldrich) in TNE buffer ...
-
bioRxiv - Microbiology 2023Quote: ... virus-containing supernatants were used to transduce A2EN cells in the presence of 8 µg/ml polybrene (Sigma-Aldrich). Cells were co-transduced with lentiviral vectors encoding the two distinct sgRNAs that target the same gene ...
-
bioRxiv - Microbiology 2024Quote: All transductions were performed via spin inoculation at 1050 x g for 2 hr at 25°C with equal virus amounts determined by Gag p24 mass in medium containing 4µg/mL polybrene (Sigma). MDMs were inoculated with 10µg p24 mass equivalents of NL4-3 ΔGPE-GFP virus or 20µg p24 mass equivalents of 3xFLAG-89.6-Vpr ...
-
bioRxiv - Microbiology 2023Quote: ... Virus supernatant was removed 6 hours post transduction and cells were maintained for 48 hours before puromycin (Sigma-Aldrich) selection ...
-
bioRxiv - Microbiology 2023Quote: ... Confluent MDCK cells were incubated with each serial dilution for 1h at 37°C then input virus was removed by aspiration and cells overlaid with SFM containing 0.14% BSA (Sigma), 0.8% Avicel© (FMC BioPolymer ...
-
bioRxiv - Immunology 2023Quote: ... Next the pseudotyped virus-containing culture medium was diluted 50% in culture medium supplemented with 8μgml−1 polybrene (Sigma) and immediately applied onto MutuDC1 cells ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were transduced with the respective virus particles in an equal ratio using 5 µg/mL polybrene (Sigma-Aldrich) according to the manufacturer’s instructions ...
-
bioRxiv - Biophysics 2023Quote: ... concentrated virus particles were freshly thawed and diluted in phosphate-buffered saline (PBS, Merck, 806552, Sigma-Aldrich, Darmstadt, Germany) based on the p24 antigen concentration in the supernatant ...
-
bioRxiv - Biochemistry 2023Quote: ... Virus-containing supernatants were harvested after 48 h transfection and filtered by syringe through a 0.45 μM filter (Millipore) to eliminate cell contaminates ...
-
bioRxiv - Developmental Biology 2023Quote: ... Cells were transduced with the virus for 48 h in the presence of 4 µg/ml polybrene (Sigma H9268).
-
bioRxiv - Microbiology 2023Quote: ... neutralized whole-virus samples were incubated in D2O buffer (99% deuterium oxide, Sigma 151882, supplemented with 120 mM NaCl). 40 μL D2O buffer was added to every 10 μL of neutralized virus (final D2O concentration 78% ...
-
bioRxiv - Cancer Biology 2023Quote: ... subconfluent cultures were incubated with virus-containing supernatants in the presence of 4µg/ml polybrene (Sigma-Aldrich , TR-1003) for 24 hours ...
-
bioRxiv - Molecular Biology 2024Quote: ... 1×107 OE19 cells were treated with virus (MOI 0.7) and 5 μg/mL Polybrene (EMD Millipore, TR-1003). Polyclonal cells were selected for 2 weeks in 500 ng/μL puromycin (Sigma ...
-
bioRxiv - Cancer Biology 2024Quote: ... Virus transduction of target sarcoma cells was performed in the presence of 8.0 μg/mL polybrene (Sigma-Aldrich, #H9268) and puromycin selection (3 μg/mL ...
-
bioRxiv - Systems Biology 2024Quote: ... the virus containing supernatant was centrifuged 500 x g for 5min and filtered by using 0.45um PES filters (Millipore). The lentivirus was concentrated by using Lenti-X concentrator (Clontec) ...
-
bioRxiv - Cell Biology 2019Quote: ... and proteins precipitated using protein-A Agarose (Sigma-Aldrich) prior to analyses by Western blotting.
-
bioRxiv - Microbiology 2022Quote: ... proteins proteins were electrophoretically transferred to nitrocellulose membranes (Millipore) using a Transblot semi-dry transfer cell (Bio-Rad) ...
-
bioRxiv - Immunology 2021Quote: ... a sandwich ELISA was used in which 1% Trixon X-100-treated virus lysates were added to plates pre-coated with ConA (50 µg/ml, Sigma) and reacted with anti-Env mAbs 66 ...
-
bioRxiv - Molecular Biology 2021Quote: ... HEC-1-B cells and HEK293T cells at the confluency of 70%∼90% were infected with the virus in the presence of 8 μg/ml polybrene (Sigma). Puromycin (Sigma ...
-
bioRxiv - Cancer Biology 2019Quote: ... were transduced when reaching around 50-70% confluence with 1x concentrated virus diluted in ADF+++ and supplemented with hEGF and 5 µg/µl polybrene (Sigma). The cells were incubated for 12-18 h with the viral suspension ...
-
bioRxiv - Cell Biology 2019Quote: ... The virus-containing medium was then used to infect RAWs in the presence of 8 μg/mL polybrene (Sigma-Aldrich) for 24 h ...
-
bioRxiv - Microbiology 2021Quote: ... The parental HEK293T cells were transduced with this virus and the HEK293T-ACE2 cell line was selected and maintained with medium containing puromycin (Sigma). ACE2 expression was confirmed by immunofluorescence staining using mouse monoclonal antibody against c-Myc antibody 9E10 (Thermo Fisher ...
-
bioRxiv - Microbiology 2021Quote: ... Virus (genome equivalent of a MOI of 1) was added in the presence of 8 μg/ml polybrene (Sigma Aldrich) and the cells were incubated on ice for 1⍰ h to allow virus binding ...
-
bioRxiv - Neuroscience 2021Quote: ... N2a cells’ media was exchanged with virus-containing supernatant at 48- and 72-hours post-transfection with 8µg/ml polybrene (Sigma #H9268). N2a cells were expanded and FACS sorted for mCherry+ cells.
-
bioRxiv - Biochemistry 2020Quote: ... The medium was replaced after 72 h and cells containing the integrated virus were selected with Puromycin (50ng/μl) (Sigma).
-
bioRxiv - Microbiology 2020Quote: ... Viruses were titrated by infecting THP-1 cells (2×105 cells/ml) with dilutions of sucrose purified virus in the presence of polybrene (8 μg/ml, Sigma) and incubating for 48 h ...
-
Potential for virus endogenization in humans through testicular germ cell infection: the case of HIVbioRxiv - Microbiology 2020Quote: ... the assay was performed on cells exposed to Pronase (3,5mg/ml) or using virus pre-exposed to 1 to 100 U/mL heparin (Sigma-Aldrich). The role of mannose receptor was evaluated by incubating cells with HIV-1 in the presence of 100µg/mL BMA (Sigma-Aldrich ...
-
bioRxiv - Microbiology 2020Quote: ... Free dyes and lipids were then purified away from labeled virus particles by ultracentrifugation for one hour at 4 °C at 40,000 rpm over a 6%-18% Optiprep (Sigma Aldrich) gradient ...
-
bioRxiv - Molecular Biology 2021Quote: HEK293FT-rKSHV cells were generated by infecting HEK293FT cells with concentrated rKSHV.219 virus (43) in the presence of 8 µg/mL polybrene (Sigma) and by spinoculation (800 g for 30 min at room temperature) ...
-
bioRxiv - Microbiology 2019Quote: ... or PMA-treated THP-1 cells (2×105 cells/ml) with dilutions of sucrose purified virus in the presence of polybrene (8 µg/ml, Sigma) for 48 h and enumerating GFP-positive cells by flow cytometry using the FACS Calibur (BD ...
-
bioRxiv - Bioengineering 2022Quote: ... HeLa cells were seeded at 25% confluency in 96 well plates and transduced with virus (lentiCRISPR-v2 with or without HPRT1 guide) and 8 μg/mL polybrene (Millipore). Virus was removed the next day and 2.5 μg/mL puromycin was added to remove cells that did not receive virus two days later ...
-
bioRxiv - Cell Biology 2022Quote: ... 50,000 MBS-MEFs were seeded in 6-well plates and exposed to the virus particles mentioned above in serum-free media supplemented with 6 µg/ml polybrene (Sigma). Cells with high or low expression of tdTomato were selected by FACS (BD Biosciences Aria III).
-
bioRxiv - Cell Biology 2022Quote: ... Cell supernatant containing the virus was collected 3 days after transfection and 20-fold concentrated using Amicon Ultra 15 10K (Millipore) centrifugal filter ...
-
bioRxiv - Cancer Biology 2022Quote: ... The same method was used for virus to be used for GSCs (DMEM was changed to NBM).The shRNA construct number TRCN0000152163 from Sigma human TRC shRNA library was used for knockdown studies of human FMOD ...
-
bioRxiv - Microbiology 2020Quote: ... Mock- and virus-infected cells were harvested at 16 h post infection and stored at −80°C in RNALater (Sigma) until RNA extraction ...
-
bioRxiv - Microbiology 2020Quote: ... Surviving midges and mosquitoes at day 10 were pooled (n=5-10) in 1 ml virus transport media (199E media supplemented with antibiotic-antimycotics; Sigma), and stored at −80°C until processed for virus isolation (VI ...
-
bioRxiv - Immunology 2022Quote: ... The supernatant was then passed through a 0.45 µm filter to remove debris and virus was concentrated using Amicon Ultra-15 Centrifugal Filter Units (Sigma, UFC9100). For this ...
-
bioRxiv - Cancer Biology 2019Quote: ... Functional titration was performed by transduction of cells with serially diluted virus in the presence of polybrene (4 μg/ml, Sigma) for 6 hours followed by puromycin (Life Technologies ...
-
bioRxiv - Cell Biology 2020Quote: ... media was removed and replaced with a mixture of culture media (50%) and crude virus (50%) containing Polybrene (8 μg/ml, Sigma). Transduction media was removed and replaced with fresh culture media 24 h later ...
-
bioRxiv - Neuroscience 2021Quote: ... was used to obtain 5×105 cells single-cells per well of a Matrigel-coated 6-well plate that were incubated with concentrated virus in mTeSR1 with 10 μM ROCK inhibitor and 8 μg/ml polybrene (Sigma) for 8 hours ...
-
bioRxiv - Neuroscience 2021Quote: ... and the virus containing supernatant was loaded on a previously with lysis buffer pre-equilibrated 1 ml HiTrap Heparin HP column (SIGMA). Several washing steps later (increasing salt conditions ...
-
bioRxiv - Microbiology 2020Quote: ... RNA was converted to cDNA using the First Strand cDNA synthesis kit for reverse transcription (avian myeloblastosis virus [AMV]; Millipore-Sigma). Quantitative real-time PCR (qRT-PCR ...
-
bioRxiv - Microbiology 2019Quote: ... of 0.01 using virus diluted in virus growth media (VGM) (DMEM supplemented with penicillin/streptomycin [Corning cellgro], 25 mM HEPES [Corning cellgro], 0.3% BSA [Sigma-Aldrich]) with 0.25 μg/ml TPCK-trypsin ...
-
bioRxiv - Microbiology 2021Quote: ... freeze-thaw cycles) was followed by concentration of virus suspensions (i.e., filtered lysates) using 30kDA MWCO spin concentrator (Sigma-Millipore). From the retentate ...
-
bioRxiv - Immunology 2020Quote: ... 400 μl containing 300 PFU/ml of SARS-CoV-2 virus were then added to the mAb solution supplemented with 0.25% guinea pig complement sera (Sigma, Israel) and the mixture incubated at 37°C ...