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Citations for Millipore Sigma :
101 - 150 of 7599 citations for Zika Virus Envelope Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Virus inoculum was removed and cells overlaid with MEM containing 1% carboxymethylcellulose (Sigma), 4% (v/v ...
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bioRxiv - Microbiology 2020Quote: ... 100 μl of virus preparations were treated with 25 units of Benzonase (Sigma) for 30 min before RNA isolation.
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bioRxiv - Cancer Biology 2020Quote: ... the virus was harvested from the medium filtered by 0.45 mm Steriflipfilter (Millipore). Virus infection was carried out by incubating cells with cell medium containing indicated virus and 5 μg/ml polybrene (Sigma ...
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bioRxiv - Microbiology 2020Quote: Cells or virus were lysed with RIPA buffer and protease inhibitor cocktail (Sigma). Protein loaded from viral lysates were normalized to p24 antigen content ...
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bioRxiv - Immunology 2021Quote: ... Virus and cell pellets were solubilized in lysis buffer [10mM iodoacetamide (Sigma-Aldrich), CompleteTM protease inhibitor tablets (Roche) ...
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bioRxiv - Immunology 2021Quote: ... The virus-plasma-cell mixtures were plated in white polystyrene plates (Millipore-Sigma) and cultured at 37°C 5% CO2 ...
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bioRxiv - Immunology 2021Quote: ... The virus-plasma-cell mixtures were plated in white polystyrene plates (Millipore-Sigma) and cultured at 37°C 5% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... Supernatant was aspirated and cells resuspended in virus medium containing TPCK-trypsin (Sigma), 20 mM HEPES (Corning ...
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bioRxiv - Microbiology 2024Quote: ... The virus-containing supernatant was filtered through 0.45-mm polyvinylidene difluoride filters (Millipore) and precipitated in 8.5% polyethylene glycol [average molecular weight (Mn) ...
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bioRxiv - Microbiology 2024Quote: ... The virus-containing supernatant was filtered through 0.45-mm polyvinylidene difluoride filters (Millipore) and precipitated in 8.5% polyethylene glycol [average molecular weight (Mn) ...
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bioRxiv - Cancer Biology 2023Quote: ... The virus containing medium with 8 μg/mL polybrene (Sigma-Aldrich, Cat #H9268) was used in 1:1 dilution with the culture medium to infect SKBR3 and HCC1954 cells ...
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bioRxiv - Neuroscience 2023Quote: ... the virus-containing medium was filtered through Costar Spin-X columns (Sigma #8162) as previously described 31.
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bioRxiv - Neuroscience 2023Quote: ... The virus was slowly mixed with silk fibroin solution (50mg/mL, Millipore Sigma) with a ratio of 1:1 through the pipette to avoid generating bubbles ...
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bioRxiv - Immunology 2023Quote: ... virus particles were precipitated by adding 50% (w/v) PEG 6000 (Sigma-Aldrich) in NT buffer (150 mM NaCl ...
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bioRxiv - Bioengineering 2023Quote: ... filtered the virus-laden media through a 0.45µm PES filter (Millipore-Sigma #SE1M003M00) and stored 1 ml aliquots in cryovials at -80°C.
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bioRxiv - Bioengineering 2023Quote: ... filtered the virus-laden media through a 0.45µm PES filter (Millipore-Sigma #SE1M003M00) and stored 1 ml aliquots in cryovials at -80°C.
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bioRxiv - Bioengineering 2023Quote: ... and Anti-Adeno-associated Virus 9 Antibody clone HL2374 (Millipore Sigma, #MABF2326-25UG). Following three additional washes ...
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bioRxiv - Cell Biology 2023Quote: ... Virus was resuspended by fresh MEFs medium with 8 μg/ml polybrene (Sigma).
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bioRxiv - Cell Biology 2023Quote: ... Virus-containing medium was passed through a 0.45 µm filter (cat. #SLHVR33RB, Millipore), aliquoted and stored at -80°C ...
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bioRxiv - Microbiology 2024Quote: ... individuals performed a nasopharyngeal swab into virus transport medium (VTM; MWE Sigma-Virucult), gave details on any recent infection episodes ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... HEK293T cells were co-transfected with library plasmids and suitable packaging- and envelope plasmids (CRISPR & MISSION® Lentiviral packaging mix, Sigma-Aldrich) by using Lipofectamine 2000 Transfection Reagent according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... 1.8 µg pCMV-VSV-G (lentiviral envelope plasmid) and 2 µg purified shRNA DNA with 12 µg (3:1) of X-tremeGENE transfection reagent (Sigma Aldrich, #6366244001). Following 72 hours of transfection ...
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bioRxiv - Cancer Biology 2024Quote: ... Lentiviral particles were prepared by transfecting 293T cells with expression clones along with the lentiviral envelope and packaging plasmids using X-tremeGENE HP DNA transfection reagent (Sigma-Aldrich 6366546001). The media was refreshed after 24 h ...
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bioRxiv - Cell Biology 2022Quote: ... EVI1 or MECOM virus in 12 well plates with 8µg/ml of polybrene (Millipore), spun at 2,000 r.p.m ...
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bioRxiv - Microbiology 2021Quote: ... the virus was concentrated using a 30-kDa Amicon Ultra-15 Centrifugal Filter (Millipore). The viral stocks were stored at -80°C ...
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bioRxiv - Microbiology 2020Quote: ... the virus inoculum was removed and phenol-red free DMEM supplemented with3% BSA (Sigma), 100U/ml pen/strep (Life Technologies) ...
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bioRxiv - Biophysics 2021Quote: ... the virus supernatant was concentrated using 8% polyethylene glycol (PEG)-6,000 (Sigma-Aldrich, U.S.A). The pellet was resuspended in TNE buffer (50 mM Tris-HCl ...
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bioRxiv - Immunology 2022Quote: ... 1 μL of virus lysate was treated with 250 U of benzonase nuclease (Sigma) at 37°C for 1 h ...
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bioRxiv - Microbiology 2022Quote: ... Virus stocks were filtered via centrifugation with Amicon Ultra-15 Centrifugal filter unit (Sigma). Infectious viral titers were quantified by plaque assay in Vero-E6 cells in MEM supplemented with 2% FBS ...
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bioRxiv - Microbiology 2022Quote: ... Plates with Towne virus were stained with mouse anti-human CMV IE1 (MAB810, Millipore) then goat anti-mouse IgG-AF488 (Millipore) ...
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bioRxiv - Cancer Biology 2022Quote: ... thawed virus was mixed with culture media +8 μg/mL Polybrene (Millipore Sigma #107689) to infect cells overnight ...
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bioRxiv - Microbiology 2021Quote: ... The virus/antibody mixture was replaced with an overly media containing 1% CMC (Sigma) before incubation for 24 h at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... The pellet was dissolved in 0.5 ml virus lysis solution (50 mM TrisHCl; Sigma), 2 mM MgCl2 (Sigma) ...
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bioRxiv - Cell Biology 2021Quote: ... virus-containing media were replaced by selection media containing 2 µg/ml puromycin (Sigma). All assays were performed 7–8 days after infection.
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bioRxiv - Microbiology 2021Quote: ... Virus were then buffer exchanged with Amicon Ultra-4 Centrifugal Unit (Millipore Sigma, #UFC800396) to get rid of furin inhibitor in the media.
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bioRxiv - Immunology 2020Quote: ... virus stocks were diluted at indicated MOIs in serum-free DMEM (D5756-500ML, Sigma). 300 μl serum-free media containing virus (or DMEM alone for the control wells ...
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bioRxiv - Cell Biology 2022Quote: ... medium containing the virus was collected and filtered with a 0.45 μm filter (Millipore). The filtered virus solution was directly used for the infection of cell lines or stored in aliquots at −80°C without prior concentration of the virus ...
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bioRxiv - Microbiology 2022Quote: ... and 1:4 800 000 in virus growth medium (VGM) containing MEM (Sigma, 2279), 2 % FBS ...
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bioRxiv - Systems Biology 2023Quote: ... we filtered the virus-laden media through a 0.45µm PES filter (Millipore-Sigma #SE1M003M00) and stored 1.5 mL aliquots in cryovials at -80ºC.
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bioRxiv - Systems Biology 2023Quote: ... we filtered the virus-laden media through a 0.45µm PES filter (Millipore-Sigma #SE1M003M00) and stored 1.5 mL aliquots in cryovials at -80ºC.
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bioRxiv - Cell Biology 2024Quote: ... Cell lines were transduced with a mix of supernatant containing virus and polybrene (Sigma H9268 suspended at 4mg/ml in sterile water ...
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bioRxiv - Cell Biology 2024Quote: ... The virus was resuspended by fresh MEFs medium with 8 μg/ml polybrene (Sigma).
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bioRxiv - Systems Biology 2023Quote: ... we filtered the virus-laden media through a 0.45µm PES filter (Millipore-Sigma #SE1M003M00) and stored 1.5 mL aliquots in cryovials at -80ºC.
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bioRxiv - Systems Biology 2023Quote: ... we filtered the virus-laden media through a 0.45µm PES filter (Millipore-Sigma #SE1M003M00) and stored 1.5 mL aliquots in cryovials at -80ºC.
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bioRxiv - Neuroscience 2023Quote: ... cells were lysed and virus was concentrated using an Optiprep density gradient (Sigma #D1556). Small-scale AAV virus was produced in HEK293T cells grown in 12-well plates by triple-transfection with 0.4μg HiUGE vector ...
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bioRxiv - Bioengineering 2023Quote: ... we filtered the virus-laden media through a 0.45µm PES filter (Millipore-Sigma #SE1M003M00) and stored 1.5mL aliquots in cryovials at -80°C.
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bioRxiv - Microbiology 2023Quote: ... and resuspending in virus + polybrene (final conc 8 ug/mL, Sigma-Aldrich, TR-1003) or RPMI media + polybrene for the uninfected control at a concentration of 1E6 cells/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... the virus-containing supernatants from HEK293 cultures mixed with polybrene (Sigma, St. Louis, MO) at a final concentration of 8 mg mL-1 were added to the cells in each well ...
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bioRxiv - Bioengineering 2023Quote: ... we filtered the virus-laden media through a 0.45µm PES filter (Millipore-Sigma #SE1M003M00) and stored 1.5mL aliquots in cryovials at -80°C.
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bioRxiv - Cancer Biology 2023Quote: ... Virus (400 µL) was added together with 5 ug/mL of polybrene (Sigma-Aldrich) and cells were centrifuged at 2,000 rpm for 2 h at 30°C ...