Labshake search
Citations for Millipore Sigma :
301 - 350 of 10000+ citations for Recombinant Zebrafish IL 1 beta Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2021Quote: The bacteriophage T7 gp16 gene was cloned into the pET-16b vector (ampr and 10x-His tag) from Novagen (Germany), inserted between NdeI and XhoI restriction sites ...
-
bioRxiv - Cell Biology 2021Quote: ... The cDNA sequence encoding 1053-1264 aa mouse was amplified and cloned upstream of sequences encoding a polyhistidine stretch (His) tag in pET23b (Novagen). The mGli2-His recombinant protein was expressed in BL21 (DE3 ...
-
bioRxiv - Bioengineering 2022Quote: ... It was cloned in the expression vector pET30 Ek/lic containing an N-terminal His-tag with the help of the ligation independent Ek/lic system according to the protocol proposed by Novagen, UK ...
-
bioRxiv - Immunology 2020Quote: ... The elimination of the N-terminal His tag was performed through the cleavage with biotinylated thrombin included in the Thrombin Cleavage Capture Kit (Merck, Millipore) following manufacturer’s instructions ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Mouse anti-FLAG M2 monoclonal antibody (F1804) and mouse anti-His-tag monoclonal antibody (D291-3S) were purchased from Sigma□Aldrich (St ...
-
bioRxiv - Biochemistry 2023Quote: The NP gene from strain A/WSN/33 (H1N1) with a His-tag at its C-terminal was cloned in the pET22 plasmid (Novagen). Single point mutation R416A was introduced through the Quick-Change kit (Stratagene ...
-
bioRxiv - Molecular Biology 2023Quote: Wild type and 2xSE variant of MpRBOHBN with an N-terminal 6 × His tag were expressed in Escherichia coli Rosetta-gami 2(DE3) (Novagen). Cells were grown at 37°C in lysogeny broth (LB ...
-
bioRxiv - Synthetic Biology 2022Quote: ... Grids were blocked in 2% BSA in TBST for 30 minutes and then moved into a 20 µl drop of anti-His tag primary antibody (Sigma) diluted 1:100 ...
-
bioRxiv - Plant Biology 2024Quote: ... 1035 bp) were cloned with Platinum™ SuperFi™ DNA Polymerase (www.thermofisher.com) into a pCDFDuet His-tag vector (Novagen, www.merckmillipore.com) using two forward primers containing SacI site and a common reverse primer with KpnI site (5′-ATTGAGCTCGATGGCGGCGAGCGAGGGCGT-3′ and 5′-ATTGAGCTCGGCCAGCAGCGGCTACGAAGGGA-3′ ...
-
bioRxiv - Molecular Biology 2024Quote: ... Protein purification steps were analyzed by 12.5% SDS-PAGE and WB with the His-Tag Antibody HRP Conjugate Kit (Merck-Millipore, Germany). Protein concentration was determined using the Bradford method (Bio-Rad ...
-
bioRxiv - Cancer Biology 2024Quote: PC4 cDNAs wild type or carrying the W89A mutation and with a N-terminal 6x His-tag sequence were cloned into a pET-Duet1 plasmid (Merck Millipore) and transformed into competent BL21 cells (Invitrogen) ...
-
bioRxiv - Microbiology 2022Quote: ... and the recombinant protein expression was induced with 0.5 mM isopropyl-D-1-thiogalactopyranoside (IPTG; Sigma-Aldrich). The bacteria were incubated at 18°C for additional 18 hours ...
-
bioRxiv - Genetics 2023Quote: ... Protein expression was verified and quantified by Western blot using recombinant GST protein (Sigma, G5663) as expression standards ...
-
bioRxiv - Cancer Biology 2024Quote: Recombinant TNC (1 µg, EMD Millipore) and Recombinant Wnt-2 (Biomatik ...
-
bioRxiv - Bioengineering 2021Quote: ... and Beta-Actin (Sigma Aldrich A1978 at 1:10000 dilution) followed by an HRP-conjugated secondary antibody and visualization by enhanced chemiluminescence.
-
bioRxiv - Neuroscience 2021Quote: ... Antibody against beta-actin from Sigma (A1978, Mouse, 1:10,000) was used as a loading control.
-
bioRxiv - Neuroscience 2021Quote: ... anti-Beta-actin (mouse monoclonal, Sigma-Aldrich, A5316, 1:5000) and anti-GFP (mouse polyclonal ...
-
bioRxiv - Cancer Biology 2021Quote: ... and anti beta actin at 1:10000 dilution (Sigma #A5316), and were visualized using monoclonal anti-Rabbit (1:3000 ...
-
bioRxiv - Cancer Biology 2022Quote: 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB) was from Sigma and was used at 100 µM for 2h ...
-
bioRxiv - Microbiology 2020Quote: ... and 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB; Sigma Aldrich) were added 8 h prior to fixation or RNA extraction ...
-
bioRxiv - Developmental Biology 2020Quote: ... Anti-beta III tubulin antibody (Sigma-Aldrich AB9354; 1:500).
-
bioRxiv - Neuroscience 2022Quote: ... Anti-Beta III Tubulin (Sigma Aldrich, 1:1000, AB9354, RRID:AB_570918), NF200 (Abcam ...
-
bioRxiv - Neuroscience 2023Quote: ... mouse IgG1 anti-Beta-catenin (Sigma, cat. C7207, 1:100), rabbit IgG anti-GluR2/3 (Millipore ...
-
bioRxiv - Cancer Biology 2023Quote: ... with or without 1:10,000 anti-beta actin (Sigma, A5441) or 1:1000 anti-GAPDH antibodies (Cell Signaling Technology ...
-
bioRxiv - Cancer Biology 2024Quote: ... mouse anti-Beta Actin (Sigma-Aldrich, Cat#A1978, 1:1000), rat anti-BrdU (Abcam ...
-
bioRxiv - Cell Biology 2024Quote: ... and anti-beta-actin (Sigma-Aldrich, ref A3854, 1:10000). Membranes were incubated with HRP-conjugated secondary antibody (Jackon ImmunoResearch anti-mouse ref 211-032-171 and anti-goat ref 115-035-146 ...
-
bioRxiv - Neuroscience 2023Quote: ... to detect SORL1 and Beta actin 1:2,000 (Sigma #A5441) as loading control ...
-
bioRxiv - Cell Biology 2023Quote: ... rabbit α-beta Catenin (IF 1:1000; C2206; Sigma Aldrich), Phalloidin ...
-
bioRxiv - Neuroscience 2023Quote: ... Beta-actin (Sigma Aldrich, St. Louis, MO; mouse, 1:10,000). All immunostaining was visualized using Alexa-fluorophore-conjugated secondary antisera (1:500 ...
-
bioRxiv - Developmental Biology 2024Quote: ... rabbit polyclonal anti-Beta Catenin (Sigma-Aldrich #C2206, 1:100), mouse monoclonal anti-Notch (DSHB C17.9C6 ...
-
bioRxiv - Neuroscience 2024Quote: ... HRP-conjugated anti-beta-actin (Sigma-Aldrich, A3850, 1:20000) was incubated for 1 hour at room temperature ...
-
bioRxiv - Cell Biology 2024Quote: ... and Chicken Anti - Beta-Actin (Sigma Aldrich, 1:150 dilution) primary antibodies were incubated overnight at 4°C ...
-
bioRxiv - Cell Biology 2020Quote: Larval and adult zebrafish were anaesthetised and mounted in 1% low-gelling agarose (Sigma). Live imaging was performed on a ZEISS Lightsheet Z.1 system with a 40x W Plan Apochromat objective or a Leica TCS SP8 AOBS confocal laser scanning microscope with a 25x/0,95 W HC FLUOTAR objective with resonant scanner (frame intervals of 0.02-0.04 seconds) ...
-
bioRxiv - Neuroscience 2020Quote: Zebrafish embryos or larvae were mounted in 1% low-melting point agarose (Sigma-Aldrich) and anesthetised using tricaine (MS-222 ...
-
bioRxiv - Neuroscience 2023Quote: ... Anaesthetized 3 dpf zebrafish larvae were mounted in 1% low-melting point agarose (Sigma), perpendicular to a glass slide in a Petri dish filled with electroporation buffer (180mM NaCl ...
-
bioRxiv - Cell Biology 2024Quote: ... Zebrafish embryos were treated with 0.2 mM 1-phenyl 2-thiourea (Sigma-Aldrich, P7629) after 24 hpf to prevent pigment formation ...
-
bioRxiv - Biophysics 2020Quote: ... the soluble His-tagged proteins were produced and purified after binding to a Ni-NTA His-Bind resin (Sigma-Aldrich, MO, US) as described by Martínez-Martínez (19) ...
-
bioRxiv - Microbiology 2020Quote: ... Mouse anti-His antibody (1:12000) (Sigma-Aldrich) and biotinylated PNA (1:6000 ...
-
bioRxiv - Cell Biology 2021Quote: ... and Anti-His(1:3000) antibody(Sigma #H1029).HRP conjugated mouse IgG was used at 1:4000 dilution(Sigma #A3673 ...
-
bioRxiv - Biochemistry 2021Quote: ... anti-His (Sigma H1029; 1:3000 for IB), anti-Hsp70 (Abcam ab47455 ...
-
bioRxiv - Biochemistry 2021Quote: ... Recombinant proteins were induced in Rosetta™ (DE3) Competent Cells (Novagen) with 0.02% (w/v ...
-
bioRxiv - Biophysics 2021Quote: ... The recombinant proteins were first purified by Ni2+-affinity column (Novagen) under native conditions and subsequently in-gel cleavage by TEV protease was conducted ...
-
bioRxiv - Cell Biology 2020Quote: ... To induce recombinant proteins isopropyl-β-D-thiogalactoside (IPTG, Sigma-Aldrich) was added (0.1 mM ...
-
bioRxiv - Cell Biology 2021Quote: Recombinant protein expression vectors were constructed from the pET28a series (Novagen) encoding an N-terminal hexa-histidine tag and thrombin cleavage site.
-
bioRxiv - Plant Biology 2023Quote: ... The recombinant fusion protein was purified with Glutathione Sepharose beads (Sigma). Definite probes were designed for different cis-elements and their mutated versions (Table S3) ...
-
bioRxiv - Developmental Biology 2024Quote: ... Recombinant protein was produced in Escherichia coli strain BL21(DE3) (Novagen). The cells were grown in LB medium at 37°C and the protein expression was induced at OD600 of 0.7 with 0.2 mM IPTG (Isopropyl β-d-1-thiogalactopyranoside ...
-
bioRxiv - Biophysics 2022Quote: ... The recombinant proteins were first purified by Ni2+-affinity column (Novagen) under native conditions and subsequently in-gel cleavage by TEV protease was conducted ...
-
bioRxiv - Developmental Biology 2023Quote: ... 103 units/mL ESGRO recombinant mouse LIF protein (Millipore Sigma, ESG1107), 2 μM PD0325901 (Tocris ...
-
bioRxiv - Evolutionary Biology 2024Quote: All recombinant proteins were expressed using pET-11a (Sigma #69436-3) based vectors in BL21(DE3 ...
-
bioRxiv - Molecular Biology 2022Quote: ... HSV tag (Sigma-Aldrich H6030, 1:1,000 or 1:2,000), Rpb1-Core (Y80 Santa Cruz ...