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Citations for Millipore Sigma :
301 - 350 of 10000+ citations for Recombinant Mouse Regenerating Islet derived 3 Alpha His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... and then with a secondary mouse anti-rabbit IgG tagged with FITC (Millipore, MA). Positively stained areas were quantified and expressed as positive area (μm2)/total area (mm2).
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bioRxiv - Systems Biology 2024Quote: ... Tagged proteins were detected using mouse α-Flag M2 (Sigma Aldrich F3165, 1:10,000), mouse α-V5-tag (Thermo Fisher R960-25 ...
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bioRxiv - Immunology 2023Quote: ... SRBCs in Alsever’s (TCS Bioscience) were conjugated to recombinant HEL3X (R. Brink) with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (Sigma) as previously described104 ...
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bioRxiv - Cell Biology 2022Quote: ... Islet architecture was analyzed using isolated mouse islets fixed in 4% PFA overnight followed by a 20 min incubation with methylene-blue solution (Sigma) and OCT embedding ...
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bioRxiv - Physiology 2019Quote: Mouse islets were isolated as previously described61 using Type XI collagenase (1000 units/mL; #C7657, Sigma-Aldrich, St. Louis, MO). Following digestion and filtration ...
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bioRxiv - Cell Biology 2020Quote: ... derived from E12.5 mouse embryos were plated and expanded on 0.1% porcine gelatin (Sigma G2500) coated dishes and irradiated (8000 rads) ...
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bioRxiv - Systems Biology 2019Quote: WT mESCs derived from inbred mouse (Bruce 4 C57BL/6J, male, EMD Millipore, Billerica, MA) and other KI derivatives were cultured on either LN511 or gelatin coated dish under either Std ...
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bioRxiv - Molecular Biology 2019Quote: ... or 3 μg mouse IgG (Sigma) and incubated rotating for 2 hours at +4°C ...
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bioRxiv - Biochemistry 2022Quote: ... alpha lytic protease (Sigma), and gluC (Sigma)-trypsin ...
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p62-DNA-encoding plasmid reverts tumor grade, changes tumor stroma, and enhances anticancer immunitybioRxiv - Cancer Biology 2019Quote: ... alpha Smoot Muscle (Sigma). Clone 1A4 (mouse Ig2A isotype) ...
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bioRxiv - Developmental Biology 2019Quote: ... Alpha MSH (Sigma; M4135) treatment was provided 6 hours post transfection of luciferase constructs.
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bioRxiv - Cell Biology 2021Quote: ... alpha-tubulin (Sigma Aldrich) and TOM1L1 (Proteintech) ...
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bioRxiv - Cancer Biology 2022Quote: ... alpha-tubulin (Sigma, T9026), BUBR1 (kindly provided by Stephen Taylor ...
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bioRxiv - Genetics 2023Quote: ... alpha-Tubulin (T6199, Sigma). Images were acquired with ImageQuant LAS4000 or LAS800 following incubation with HRP-conjugated IgG (Invitrogen) ...
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bioRxiv - Bioengineering 2023Quote: ... and alpha-tocopherol (Sigma), which served as an antioxidant stabilizer ...
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bioRxiv - Cell Biology 2023Quote: ... alpha-actinin (Sigma, A7811), Vinculin (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... Immunoprecipitation of 3×Flag-tagged proteins was performed using α-FLAG M2 affinity gel (Sigma-Aldrich) following the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2022Quote: ... induction of AID-tagged protein depletion was performed with 3-indole-acetic acid (IAA) (Sigma-Aldrich) during the exponential growth phase (approx ...
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bioRxiv - Microbiology 2021Quote: Full-length CA sequences derived from pNL4-3 was introduced to pET30a vectors (Novagen-Merck KGaA, Germany), producing a pET30a CA vector ...
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bioRxiv - Microbiology 2022Quote: ... Sub-confluent T175 flasks of 293T cells (human embryonic kidney cell line) were transfected with the RBD-His tagged plasmid using X-tremeGENE 9 reagent (Millipore Sigma, Cat# 6365809001). The supernatant was collected six days post-transfection and filtered through 0.22μm PES membrane filters (Nalgene) ...
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bioRxiv - Microbiology 2021Quote: ... Sub-confluent T175 flasks of 293T cells (human embryonic kidney cell line) were transfected with the RBD-His tagged plasmid using X-tremeGENE 9 reagent (Millipore Sigma, Cat# 6365809001). Supernatant was collected 6 days post-transfection and filtered through 0.22 μm PES membrane filters (Nalgene) ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was then added to 3 mL of HIS Select Nickel affinity gel (Sigma) equilibrated in wash buffer (10 mM HEPES ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was then added to 3 mL of HIS Select Nickel affinity gel (Sigma) equilibrated in wash buffer (10 mM NaPO4 ...
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bioRxiv - Developmental Biology 2021Quote: ... regenerating and normal developing embryos were fixed in 4% PFA/PBS and dehydrated in increasing concentrations of glycerol (Sigma-Adrich). Images were acquired Leica MZFLIII fluorescence stereo microscope with a 1x objective fitted with a
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bioRxiv - Genetics 2020Quote: Isolated islets were lysed in RIPA buffer (Sigma R0278) supplemented with protease and phosphatase inhibitors ...
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bioRxiv - Cell Biology 2020Quote: ... cells/islets were treated with 100 μM carbenoxolone (Sigma), 20 μM verapamil (Sigma) ...
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bioRxiv - Physiology 2019Quote: ... islets were treated with 0.5 mM cpt-cAMP (Sigma) in culture media containing 11 mM glucose after overnight recovery from isolation ...
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bioRxiv - Physiology 2020Quote: ... Islets were incubated overnight in 1640 RPMI Medium (Sigma) with 10% FBS ...
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bioRxiv - Physiology 2023Quote: ... Islets were isolated using Histopaque® (10771, Sigma-Aldrich) gradient ...
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bioRxiv - Cell Biology 2020Quote: The antibodies used in this study are as follows: alpha-tubulin (mouse mAb DMIA; T9026; Sigma), Arl13b (mouse mAb N295B/66 ...
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bioRxiv - Biochemistry 2021Quote: ... the membrane was re-probed using mouse anti-alpha-tubulin antibody (DM1A, Sigma Aldrich, 1:1000).
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 1:10000 dilution of anti-mouse alpha-Tubulin (Clone B-5-1-2; SIGMA) for 1 to 3 h at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... and anti-alpha tubulin primary antibodies (monoclonal mouse clones B-5-1-2 and DM1a, Sigma), revealed with donkey anti-mouse secondary antibodies conjugated to Alexa Fluor 555 ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 1000 units/ml ESGRO Recombinant Mouse LIF protein (Millipore #ESG1107), 10% fetal bovine serum ...
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ZMYM2 is essential for methylation of germline genes and active transposons in embryonic developmentbioRxiv - Molecular Biology 2022Quote: ... media supplemented with 1000U/mL ESGRO Recombinant Mouse LIF (Millipore Sigma, ESG1107), 2 mM GlutaMAX (Gibco ...
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bioRxiv - Molecular Biology 2023Quote: ... and with recombinant mouse Leukemia Inhibitory Factor (LIF, 20 U/ml, Millipore).
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bioRxiv - Developmental Biology 2023Quote: ... and 10 μg/μl recombinant mouse leukemia inhibitory factor (LIF; Sigma-Aldrich) in a 5% CO2 incubator at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... at −20°C prior to quantification of glucagon secretion in both human and mouse islets using radioimmunoassay (Millipore-Sigma, GL-32K).
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bioRxiv - Cell Biology 2024Quote: ... at −20°C prior to quantification of glucagon secretion in both human and mouse islets using radioimmunoassay (Millipore-Sigma, GL-32K).
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bioRxiv - Molecular Biology 2023Quote: ... myc-tagged TEV protease was detected with a mouse monoclonal anti-myc antibody (Sigma M4439). The proteins were detected using SuperSignal™ West Femto Maximum Sensitivity Substrate (ThermoFisher ...
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bioRxiv - Developmental Biology 2022Quote: Lentiviral particles encoding Flag-tagged human PDHA1 or shRNAs targeting mouse Pdha1 (TRCN0000041914/TRCN0000041914 (Sigma)) were produced in 293T packaging cells by transient transfection using Jet-PEI reagent (Ozyme) ...
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bioRxiv - Biochemistry 2021Quote: ... His-tagged CLIP-170 fragments and mutants were expressed in BL21 (DE3) and purified by the standard His-tagged purification protocol from Novagen (69670-5, Sigma-Aldrich) with the following modifications ...
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bioRxiv - Biochemistry 2023Quote: Thermal stability of BSA and HSA variants (His-tagged form) and of commercial wild type HSA and BSA (Sigma-Aldrich A1653 and A7638, respectively) was measured by nanoscale differential scanning fluorimetry (nanoDSF) ...
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bioRxiv - Microbiology 2020Quote: ... 1:50 polyclonal antibody against full-length human C1QBP (α-HsC1QBP) derived from mouse (Sigma-Aldrich), or both overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... The stripped membrane was re-blotted for a reference protein using 1:5000 monoclonal mouse anti-mouse alpha-tubulin (Sigma, T6199-25UL), overnight at 4°C ...
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bioRxiv - Developmental Biology 2019Quote: ... expression of Flag-tagged UNC-3 in the IP was detected using rabbit anti-Flag (Sigma, #SAB4301135). Immunoprecipitation of Flag-tagged UNC-3 was performed using Flag antibody coated beads (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... bound proteins were detected by immunoblotting with primary mouse anti-His (Sigma□Aldrich, Taiwan) or anti-GST primary antibodies (Sigma□Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... and dilutions were as follows (in parenthesis): His (mouse, Sigma cat# H1029; 1:2,500); β-actin (rabbit ...
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bioRxiv - Molecular Biology 2023Quote: ... The expression of 3 × FLAG-tagged MpRBOHB proteins was confirmed by western blot analysis using an anti-3 × FLAG antibody (Sigma-Aldrich, Cat. No. F1804).
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bioRxiv - Microbiology 2019Quote: ... we performed growth curves in YPD liquid medium containing different concentrations of Gal-3 (Gal-3 human recombinant, expressed in E. coli, Sigma-Aldrich) in a 96-well plate (Costar ...