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Citations for Millipore Sigma :
151 - 200 of 10000+ citations for Recombinant Mouse Regenerating Islet derived 3 Alpha His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Sph2-YM4271 cells were grown on synthetic dropout (SD)-His media supplemented with the inhibitor 3 mM 3-amino-1,2,4-triazole (3-AT; Sigma). All Y1H effector plasmids were separately transformed into the Sph2-YM4271 reporter strain and plated on SD/-Leu plates ...
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bioRxiv - Molecular Biology 2020Quote: ... Cultured intact mouse or human islets were resuspended in 0.1mmol/L EGTA (Sigma-Adlrich, 234626) in serum free CRMLfollowed by transduction of indicated adenoviral constructs at 10,000 plaque-forming unit per islet equivalent (pfu/IEQ ...
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bioRxiv - Developmental Biology 2022Quote: Islets were isolated from mouse pancreas using perfusion with the Liberase enzyme blend (Sigma Aldrich) as described (Tschen et al ...
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bioRxiv - Cancer Biology 2019Quote: ... Alpha-tubulin (mouse monoclonal, clone DM1A, #T9026, Sigma, Poole, Dorset, UK) was used as loading control ...
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bioRxiv - Biophysics 2019Quote: ... 1:1000 for mouse anti-alpha Tubulin (Sigma Clone DM1A T9026), or 1:1000 for rabbit anti-beta Tubulin (Abcam ab6046) ...
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bioRxiv - Neuroscience 2020Quote: ... and tubulin (mouse anti-alpha-tubulin, Sigma-Aldrich, T5168; 1:250) colabeling was performed overnight with 1% NDS and 0.2% Triton X-100 in 1× PBS ...
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bioRxiv - Microbiology 2020Quote: ... and 1:1,000 mouse anti-alpha tubulin (T5168 Sigma–Aldrich USA) in 2% BSA-TTBS as loading control at concentration and were washed 3 times with TTBS ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-alpha tubulin DM1a (Sigma Aldrich, T6199, WB 1:5000), mouse anti-NeuN A60 (Millipore ...
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bioRxiv - Cell Biology 2021Quote: ... and a mouse monoclonal anti-alpha-tubulin (1:2500, Sigma-Aldrich) followed by anti-rabbit and mouse antibodies coupled to HRP (1:2000 ...
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bioRxiv - Neuroscience 2021Quote: ... mouse-anti-alpha smooth muscle actin Cy3 (Sigma, C6198; 1:500), rat anti-LAMP1 (DSHB ...
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bioRxiv - Genomics 2019Quote: ... and with mouse monoclonal anti-sarcomeric alpha-actinin antibody (Sigma, A7811), or with rabbit polyclonal anti-MLC2V (Proteintech ...
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bioRxiv - Evolutionary Biology 2020Quote: ... mouse anti-alpha-tubulin (clone DM 1A, Sigma, diluted 1:200), mouse anti-histone H3S10ph (Abcam ...
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bioRxiv - Developmental Biology 2023Quote: ... Mouse anti-alpha tubulin (DM1A) monoclonal antibody (1 : 5000, Sigma, T9026) was used as loading control ...
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bioRxiv - Physiology 2023Quote: ... and mouse anti-alpha-smooth muscle actin Cy3 (1:250; Sigma), diluted in the blocking solution ...
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bioRxiv - Molecular Biology 2023Quote: ... mouse monoclonal anti-alpha-Tubulin (1:5000, Sigma-Aldrich, cat# T6074) rabbit polyclonal anti-Histone H3 (1:5000 ...
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bioRxiv - Molecular Biology 2024Quote: ... mouse anti-alpha tubulin (DM1A) monoclonal antibody (1:10,000, #T9026, Sigma), mouse anti-Actin monoclonal antibody (1:5,000 ...
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bioRxiv - Cell Biology 2022Quote: ... mouse α-His-HRP (A7058, Sigma-Aldrich, 1:10000).
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bioRxiv - Neuroscience 2019Quote: ... interleukin alpha (IL-1a) (3 ng/ml; Sigma-Aldrich; Cat. No. I3901-5UG) and complement component 1 ...
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bioRxiv - Neuroscience 2020Quote: ... Nectin-3 alpha was PCR amplified (KOD hot start DNA polymerase, Sigma-Aldrich) from a mouse brain cDNA library using the forward primer ...
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bioRxiv - Biochemistry 2020Quote: ... The proteins were cleaved by incubation with 1.5 % (w/w) His-tagged HRV-3C protease (Millipore Sigma SAE0045) or 15 U/mg His-tagged SUMO protease (Millipore Sigma SAE0067 ...
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bioRxiv - Biochemistry 2021Quote: A C-terminal 10x His-tagged Synechocystis PCC 6803 ocp gene (slr1963) was cloned in a pCDFDuet (Novagen), resulting in a plasmid named pEP4 ...
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bioRxiv - Microbiology 2023Quote: ... Fractions containing the His-tagged nucleotidases were pooled and concentrated using an Amicon 10 kDa MWCO filter (Millipore). The protein was buffer-exchanged into gel filtration buffer (100 mM HEPES pH 7.4 ...
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bioRxiv - Molecular Biology 2024Quote: ... The peak corresponding to mono-SPY-His-tagged streptavidin tetramer was collected and concentrated using Amicon 10K (Millipore). The concentrated mono-SPY-His6-tagged streptavidin tetramer was further purified through Hiload superdex75 (Cytiva ...
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bioRxiv - Bioengineering 2023Quote: ... His-tagged GA-MatryoshCaMP6s were eluted in 1.5 mL 250 mM imidazole (Sigma-Aldrich; CAS 1467-16-9) 20 mM MOPS pH 7.0 ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies used for protein quantification for ELISAs were mouse monoclonal anti-His (His-Tag mAb, EMD-Millipore), and biotinylated mouse anti-rat Cd4 (clone OX68) ...
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bioRxiv - Cancer Biology 2021Quote: ... 500,000U Recombinant Mouse LIF Protein (Millipore Cat#ESG1107)) with feeders ...
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bioRxiv - Microbiology 2021Quote: ... 50 ng/ml mouse recombinant EGF (Sigma-Aldrich), 1X B27 supplement (Gibco) ...
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bioRxiv - Cell Biology 2022Quote: ... recombinant mouse EGF (0.025μg/ml, Sigma-Aldrich, #SRP3196) and bovine pituitary extract (30μg/ml ...
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Role of the Topoisomerase IIα Chromatin Tether domain in Nucleosome Binding & Chromosome SegregationbioRxiv - Cell Biology 2021Quote: Recombinant S-tagged TopoII-CTD proteins were loaded on S-protein Agarose beads (#69704, EMD Millipore/ Novagen) by incubating them together overnight ...
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Role of the Topoisomerase IIα Chromatin Tether domain in Nucleosome Binding & Chromosome SegregationbioRxiv - Cell Biology 2021Quote: Recombinant S-tagged TopoII-CTD proteins were loaded on S-protein Agarose beads (#69704, EMD Millipore/ Novagen) by incubating them together overnight ...
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bioRxiv - Biochemistry 2023Quote: ... FLAG-tagged recombinant substrates were purified by immunoaffinity chromatography using the anti-FLAG M2-agarose beads (Sigma), according to the manufacturer’s protocols ...
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bioRxiv - Physiology 2020Quote: ... Insulin release from islets was measured as described with Rat/Mouse Insulin ELISA kit (Millipore Sigma) (26).
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bioRxiv - Neuroscience 2019Quote: ... mouse anti-alpha-tubulin ([B-5-1-2], Sigma, T5168, RRID AB_477582) and ATTO647N-conjugated anti-GFP nanobodies (GFPBooster-ATTO647N ...
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bioRxiv - Biophysics 2022Quote: ... we use two different primary antibodies: mouse anti alpha-tubulin (Sigma T6199) 1:500 and rat anti alpha-tubulin (abcam ab6160 ...
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bioRxiv - Cell Biology 2023Quote: ... mouse monoclonal against alpha-tubulin (1:1000; b5-1-2, Sigma, T6074). Actin was stained using 100 nM of phalloidin-iFluor488 for 30 min ...
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bioRxiv - Cell Biology 2020Quote: ... Islets were recorded in DMEM (Sigma D5030 ...
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bioRxiv - Biochemistry 2024Quote: ... FLAG tagged PFD5 was detected using anti-FLAG mouse antibody (Sigma) and cMyc using anti-c-Myc rabbit antibody clone 7E18 (Sigma) ...
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bioRxiv - Neuroscience 2021Quote: ... and regenerating axons were visualized by staining with a chicken anti-Neurofilament H antibody (Millipore Sigma). Regenerating axons were quantified at specified distances from the injury site using the Sholl analysis plugin of Fiji ImageJ.
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bioRxiv - Microbiology 2022Quote: ... Proteins were detected using α -His from mouse (Sigma H1029), α-Gfp from mouse ...
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bioRxiv - Microbiology 2020Quote: ... the mice were injected with 700 μg of His-tagged TGT complex protein in incomplete Freund’s adjuvant (Sigma-Aldrich). One week after the 6-week injection ...
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bioRxiv - Microbiology 2023Quote: An N-terminal His-tagged fusion of HcpGA1 was constructed by cloning QR305_02952 into the NdeI–BamHI sites of pET16b (Novagen), and the plasmid was transformed into E ...
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bioRxiv - Biochemistry 2024Quote: ... with a TEV cleavage site and a second ribosomal binding and multiple cloning site (pABLO TEV) and His-tagged constructs were cloned into a modified form of pRSF_Duet-1 (Novagen) with a TEV cleavage site following the hexahistidine tag ...
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bioRxiv - Bioengineering 2019Quote: A mouse chondrogenic cell line (ATDC-5, derived from teratocarcinoma AT805, Sigma) and a primary human lung fibroblast cell line (FB ...
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bioRxiv - Cell Biology 2020Quote: ATDC5 cells (mouse 129 teratocarcinoma-derived osteochondral progenitors; cat# 99072806, Sigma-Aldrich)
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bioRxiv - Synthetic Biology 2021Quote: ... cerevisiae were cloned into 10×His-MBP-SUMO-nup-SNAP constructs (Figure 2A) via a pET-28a-derived vector (Novagen), and expressed in E ...
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bioRxiv - Microbiology 2020Quote: ... was cloned as 10× His-MBP-SUMO-NUP153 (or NSP1)-SNAP constructs (Figure S1) into a pET-28a-derived vector (Novagen) and expressed in BL21-Gold (DE3) ...
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bioRxiv - Developmental Biology 2021Quote: Samples from SC-islets and human islets were chemically fixed with 2.5% glutaraldehyde (EM-grade, Sigma-Aldrich) in 0.1 M sodium cacodylate buffer ...
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bioRxiv - Cancer Biology 2022Quote: Nthy-ori 3-1 cell line derived from follicular thyroid epithelium (Sigma - Aldrich, 90011609) was cultured in Cell Culture Treated Flasks at 37°C in the presence of 5% CO2 in RPMI-1640 medium containing 2mM glutamine (Paneco ...
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bioRxiv - Molecular Biology 2022Quote: ... THP-1-derived macrophages were treated with DMSO or 3 mM Itaconic Acid (Sigma) for 10 hours prior to actinomycin D treatment ...
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bioRxiv - Cell Biology 2022Quote: ... recombinant FLAG-tagged zebrafish Grk1a and Grk1b were each inserted into the pFLAG-CMV2 vector (#E7033, Millipore-Sigma). HEK-293 cells cultured in DME/F-12 with 10% fetal bovine serum were transfected with either of these constructs using FuGENE 6 Transfection Reagent (#E2691 ...