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Citations for Millipore Sigma :
301 - 350 of 10000+ citations for MEK Kinase Kinase 1 MAP4K1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... MEK signalling was inhibited using 7.5-10μM PD0325901 (Sigma-Aldrich), vehicle control groups were exposed to 0.75-1% DMSO ...
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Inhibiting the prostaglandin transporter PGT induces non-canonical thermogenesis at thermoneutralitybioRxiv - Physiology 2019Quote: ... Serum was collected and serum creatine kinase activity was measured according to manufacturer’s instructions (Cat# MAK116, Sigma-Aldrich, St. Louis, MO, USA)
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bioRxiv - Cell Biology 2022Quote: ... were used as a substrate for phospho-PKCα in a 25 µl in vitro kinase reaction using 100 ng of active recombinant PKCα enzyme (Millipore-Sigma, MA), 5 µl of a lipid activator (Millipore-Sigma ...
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bioRxiv - Biochemistry 2019Quote: ... an ATP regeneration system (2 mM ATP, 30 mM phosphoenol pyruvate and 30 U of pyruvate kinase) was used (Sigma-Aldrich, USA). For the inverse reaction ...
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bioRxiv - Microbiology 2020Quote: ... Resulting primary cells were first cultured in the selective media to proliferate in the presence of the Rho-associated protein kinase (ROCK) inhibitor (Y-27632, 10 µg/ml, Sigma-Aldrich, Poland) (95) ...
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bioRxiv - Biochemistry 2023Quote: We performed the phosphorylation kinetics presented in Figure 4a using commercial recombinant kinases GST-p38βat 10 μg/mL (Sigma-Aldrich, ref. B4437), GST-Erk2 at 20 μg/mL (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... Single-cell iAT2s were plated on Transwells at a density of 520,000 cells/cm2 in 100 μl of CK+DCI medium containing 10 μM of Rho-associated kinase inhibitor (“Y”; Sigma Y-27632). 600 μl of this medium was added to the basolateral compartment ...
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bioRxiv - Systems Biology 2023Quote: ... and resuspended in 40 µl kinase buffer containing 300 µM ATP with 6 µCi γ-32P-ATP (Hartmann analytics) and 10 U bovine PKA (Sigma, 539576). For no PKA control ...
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bioRxiv - Microbiology 2022Quote: ... kinase assays were performed by incubating the immunoprecipitated proteins and beads in 30 μl kinase buffer supplemented with 50 μM ATP and 3 μg histone H1 (EMD Millipore, 14-155) for 30 min at room temperature ...
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bioRxiv - Biophysics 2023Quote: Inhibition of Rho-kinase on NK92 cells (Fig. 4) is performed with Y-27632 Rock-inhibitor (Sigma-Aldrich, cat. no. 129830-38-2). Y-27632 Rock-inhibitor is dissolved in DMSO (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... and the second parotid salivary gland was analyzed using the pyruvate kinase activity assay kit (cat. No. MAK072, Sigma-Aldrich, St. Louis, MO). For both activity assays ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The purified BGLF4 was then used for kinase inhibition assays using the C-terminal fragment peptide of retinoblastoma protein (RB) as substrate (Millipore Sigma cta# 12-439). The remaining assay parameters were the same as those applied for PknB except for the following changes ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The plasmid that contains the kinase domain of human HASPIN was transformed into Escherichia coli strain Rosetta™ 2(DE3) pLysS (Novagen, Madison, WI, USA). The transformed cells were grown at 37□ until OD600 reached 0.8 ...
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bioRxiv - Neuroscience 2023Quote: ... in combination with anti-Ca2+/calmodulin-dependent protein kinase II α (CKII; monoclonal mouse IgG1, 05-532, clone 6G9, EMD Millipore Corporation, Temecula, USA), diluted 1:4000 ...
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bioRxiv - Molecular Biology 2020Quote: Hep-2 target cells were incubated with increasing concentrations of anti-EGFR antibodies (0 to 500 ng/mL) in the presence of Jurkat CD16 cells and the protein kinase C activator phorbol- myristate acetate (P8139, Sigma-Aldrich, St Louis, MO, USA). After 16 h of incubation at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Penicillin/Streptomycin, LIF and 2i (3 μM Gsk3 inhibitor CT-99021, 1 μM MEK inhibitor PD0325901) and Vitamin C (Sigma) at a final concentration of 100 μg/mL ...
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bioRxiv - Molecular Biology 2023Quote: ... Penicillin, Streptomycin, LIF and 2i (3 μM Gsk3 inhibitor CT-99021, 1 μM MEK inhibitor PD0325901) and Vitamin C (Sigma) at a final concentration of 100 μg/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... JNK inhibitor SP600125 and MEK inhibitor Trametinib were diluted in corn oil (Sigma, C8267). For the 4-drug combinatorial treatment ...
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CDK5 activity in retinal pigment epithelium contributes to gap junction dynamics during phagocytosisbioRxiv - Cell Biology 2023Quote: ... 30 min with MEK inhibitor PD 98059 (50 µM, Cat. no. 513001, Sigma-Aldrich), 30 min with the PKC activator Phorbol 12-myristate 13-acetate (PMA ...
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bioRxiv - Neuroscience 2020Quote: A stock solution (74.8 mM) of the MEK inhibitor 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one (PD98059, PD hereafter, Sigma-Aldrich, Argentina) was conserved at −20 °C and freshly diluted in DMSO to the desired concentration (11.22 mM ...
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bioRxiv - Neuroscience 2020Quote: The MEK inhibitor α-[amino[(4-aminophenyl)thio]methylene]-2-(trifluoromethyl)benzeneacetonitrile (SL327) (Sigma-Aldrich) was dissolved in a vehicle solution of 2.5% dimethyl sulfoxide (DMSO)/ 2.5% Cremophor EL saline solution at 30 mg/kg (Satoh et al. ...
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bioRxiv - Cell Biology 2022Quote: ... inhibition of MEK was started on d12 of differentiation by supplementing the medium with 0.1 and 0.2 μM PD0325901 (Sigma, #PZ0162). Medium was exchanged every two days until d20 ...
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bioRxiv - Cell Biology 2020Quote: ... MEK, Akt1, Akt2, or Akt3 for 72 h, or treated with FTY720 phosphate (FTY720-P, Cayman: 10008639) or triciribine (Sigma, T3830) as indicated ...
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bioRxiv - Cell Biology 2020Quote: ... solutions of PVDF-TrFE (70/30) (400 kDa., PDI of 2.1, Solvay Solexis, Inc., Thorofare, NJ) in methyl ethyl ketone (MEK; Sigma-Aldrich, Inc.) was loaded into a syringe with an 18-gauge needle ...
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bioRxiv - Biochemistry 2020Quote: ... OC antibody of 1:25,000 and A11 antibody of 1:500 (Millipore and Life Technologies ...
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bioRxiv - Bioengineering 2020Quote: ... PCM-1 antibody (1:400, Sigma-Aldrich) and concentration matched rabbit IgG isotype control (Novus Biologicals ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and then incubated with anti-CDK2 monoclonal antibody (1:100) and anti-SCP1 polyclonal antibody (NB300-229, Novus, 1:100) or anti-RAD51 polyclonal antibody (pc130, Millipore, 1:100) and anti-SYCP-3 monoclonal antibody (sc-74569 ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies (PSD95 1:500, Antibodies Inc., Davis CA and MAP2 1:1000; Millipore) were diluted in 1x PBS with 1% BSA and 0.2% Tween ...
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bioRxiv - Physiology 2019Quote: ... Pax7 primary antibody (1:100, DSHB) and laminin primary antibody (1:100, Sigma-Aldrich) were diluted in 1% TSA blocking buffer and applied overnight ...
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bioRxiv - Neuroscience 2020Quote: ... Antibodies: Trim28 (Millipore, 1:500), IAP-gag (a kind gift from Bryan Cullen and described in (Dewannieux et al. ...
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bioRxiv - Neuroscience 2020Quote: ... GluA2 antibody (1:2,000, Millipore), pGluA1-Ser845 antibody (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... β-actin antibody (1:20,000, Sigma), GAPDH antibody (1:20,000 ...
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bioRxiv - Neuroscience 2020Quote: ... Abcam),Tubulin antibody (1:1,000, Millipore) at 4℃ for 12h ...
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bioRxiv - Neuroscience 2022Quote: ... Antibody SMI32 (Millipore; 1:10,000) recognizes non-phosphorylated neurofilament protein in axons ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies included rhodopsin (1:1 000, Sigma), sw-opsin (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... mitoProfile total OXPHOS Human WB antibody cocktail (1:1000, ab110411), SDHA monoclonal antibody (1:10000, 459200), anti-TOMM20 (1:1000, HPA011562) and actin antibody (1:1200, Sigma A2066). Four 5 min washes with PBS-T (0.005% tween ...
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bioRxiv - Developmental Biology 2021Quote: ... The antibodies: Chicken anti-GFP antibody (ab16901, 1:500; Millipore), Mouse anti-Satb2 (ab51502 ...
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bioRxiv - Systems Biology 2020Quote: ... Antibodies used included: Rac1 antibody (Millipore, clone 238A, 1:500), anti-RhoA antibody (Santa Cruz Biotechnology 26C4 ...
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bioRxiv - Biochemistry 2019Quote: ... 1 μL of antibody (α-Flag-M2 antibody, Sigma-Aldrich / α-HA ...
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bioRxiv - Neuroscience 2023Quote: ... secondary antibodies (1:1000) and DAPI (D9542-1, Sigma, 1:10,000) were incubated for 2hrs at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... for 1.5 h at RT and incubated overnight at 4°C with primary antibodies: anti-dystrophin antibody (NCL-Dys1, 1:40, Novocastra Laboratories) and anti-alpha actinin antibody (A7732, 1:3000, Sigma-Aldrich). Membranes were incubated with the secondary antibody sheep anti-mouse IgG (H+L ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies included: PGP9.5 (1:1000; AB1761-1, Millipore), ATF3 (1:400 ...
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bioRxiv - Bioengineering 2022Quote: ... Primary lubricin antibody (1:400, 1% BSA, Millipore MABT401) was incubated for 90 minutes at RT ...
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bioRxiv - Neuroscience 2021Quote: ... GFP primary antibody (1:500; Millipore, product # NB600-308 [RRID:AB_10003058] ...
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bioRxiv - Molecular Biology 2021Quote: ... SFPQ antibody (1:60, WH0006421M2, Sigma) and PGBD1 antibody (1:200 ...
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bioRxiv - Microbiology 2019Quote: ... The secondary antibody (Sigma, 1:10,000) was then applied and samples were incubated at RT for 1 h ...
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bioRxiv - Microbiology 2019Quote: ... anti-VSVG antibody (1:2,000; Sigma). Immunoreactive bands were visualized by incubation with horseradish peroxidase-conjugated goat anti-rabbit immunoglobulins (1:5000 ...
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bioRxiv - Neuroscience 2019Quote: ... the NF200 antibody (Sigma, 1:500) was added to hiPSCd-sensory neurons overnight at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... MPST primary antibody (Sigma, 1:50) was incubated overnight at 4°C ...
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Orchestration of alternative splicing regulates bone marrow mesenchymal stem cells fate during agingbioRxiv - Cell Biology 2022Quote: ... nestin antibody (Millipore, MAB353, 1:100) over night at 4°C ...