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Citations for Millipore Sigma :
251 - 300 of 10000+ citations for MEK Kinase Kinase 1 MAP4K1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... The coupled enzymatic reaction contained ~2 units of pyruvate kinase/lactic dehydrogenase (rabbit muscle isolate mixture, Sigma Aldrich) 50 mM KPi ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were maintained overnight in mTeSR1 medium containing the Rho-associated kinase (ROCK) inhibitor Y-27632 (Sigma Aldrich) until cells reached 60–70% of confluency ...
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bioRxiv - Physiology 2023Quote: ... USA) fitted onto a 96-well plate and calibrated with a standard curve of purified creatine kinase (Sigma, Oakville ...
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bioRxiv - Cancer Biology 2023Quote: ... Dexamethasone and MEK inhibitor PD-184352 (Sigma-Aldrich). Cell viability assays were performed as previous reported Pickard [46] ...
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bioRxiv - Neuroscience 2023Quote: ... PD98059 (MEK inhibitor; Sigma Aldrich, #P215; 20 μM) or rapamycin (mTORC1 inhibitor ...
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bioRxiv - Developmental Biology 2019Quote: ... into single-cell suspension and re-suspended in E8 media containing 10 μM Rho-associated protein kinase inhibitor (Sigma). Approximately 100,000 cells were re-plated into Matrigel-coated 6-well plates ...
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bioRxiv - Molecular Biology 2020Quote: ... Singlecell iAT2s were plated on Transwells at a density of 520,000 live cells/cm2 in 100μl of CK+DCI with 10μM Rho-associated kinase inhibitor (“Y”; Sigma Y-27632). 600μl of CK+DCI+Y was added to the basolateral compartment ...
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bioRxiv - Microbiology 2020Quote: ... with 0.5mM nicotinamide adenine dinucleotide (NADH) and 2mM phosphoenolpyruvate and 3µl of a pyruvate kinase/lactate dehydrogenase mix (PYK/LDH; Sigma). PomXNPEP and PomXNPEP_K13AR15A peptides (MKKAFEQNVSRAKPRLRLGALT and MKKAFEQNVSRAAPALRLGALT ...
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bioRxiv - Bioengineering 2022Quote: ... and a medium with an extracellular signal-regulated kinase inhibitor (ERKi, MilliPore Sigma #1049738-54-6, Burlington, MA, USA), adapted from Eigler et al.2 More details on the media used are provided in Supplementary Table 1.
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bioRxiv - Developmental Biology 2020Quote: ... per manufacturer’s instructions and seeded onto the micropattern at 132,000 – 263,000 cells/cm2 in mTeSR with 10 μM Rho-associated kinase inhibitor (ROCKi Y-27632, Millipore Sigma). After 6h ...
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bioRxiv - Cell Biology 2020Quote: ... 2005) in which the final concentrations of coupling reaction components were adjusted as follows: Pyruvate kinase (Sigma Aldrich, P9136) – 400 units/mL ...
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bioRxiv - Cell Biology 2021Quote: ... Sorted cells were cultured in Matrigel® drops with ENR_CV-medium plus the Rho Kinase inhibitor Y-27632 (Sigma) to obtain a new in vitro line of intestinal organoids named Lgr5-EGFP/RCL-tdT ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 2004) and incubated overnight in 100 ml reaction buffer (kinase buffer + 0.2 mg/ml BSA (BSA Fraction V, Sigma) + 50 µg/ml kanamycin) ...
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bioRxiv - Microbiology 2024Quote: ... 10 mM magnesium chloride pH 7.4) and incubated overnight in 100 ml reaction buffer (kinase buffer + 0.2 mg/ml BSA (BSA Fraction V, Sigma) + 50 μg/ml kanamycin) ...
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bioRxiv - Molecular Biology 2021Quote: ... and cultured in F-media (62) containing 10 μM Y-27632 Rho-kinase inhibitor (63) in the presence of mitomycin C (M4287; Sigma) treated 3T3 J2 fibroblasts ...
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bioRxiv - Biochemistry 2020Quote: ... phosphorylation of peptides by the active Pro-directed kinase GST-CDK2/Cyclin A2 (100 ng purified from Sf9 cells, Sigma) was also confirmed in the presence or absence of the ATP competitive inhibitor ...
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bioRxiv - Biophysics 2021Quote: COS-7 cells transfected with WT tau alone or with fyn kinase were similarly stained with tau-5 (Sigma-Aldrich) or with tau-9g3 primary antibody (GeneTex Inc. ...
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bioRxiv - Cell Biology 2021Quote: ... splenic B-cells from MD4 mice were pretreated or not with 5 μM of the Src kinase inhibitor PP2 (Millipore) (Cheng et al ...
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bioRxiv - Cell Biology 2021Quote: ... Cdc5-3V5 and Rim4-3V5 (control non-kinase tagged protein) were immunoprecipitated at 4°C 2 hours using 20 µL of anti-V5-agarose slurry (Sigma). Purifications were washed 4 times with NP40 buffer.
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bioRxiv - Systems Biology 2021Quote: Cell fractions were thawed on ice and endogenous kinases were inhibited with 5′-[p-(fluorosulfonyl)benzoyl]adenosine (FSBA; Sigma-Aldrich) in DMSO at a final concentration of 1 mM FSBA and 10% DMSO in Tris-HCL ...
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bioRxiv - Plant Biology 2022Quote: AMPK3 and 4 activity was determined using a coupled enzyme assay with pyruvate kinase and lactate dehydrogenase (LDH, Sigma, P0294), measuring the production of NAD+ that results in absorption-decrease at 340 nm ...
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bioRxiv - Molecular Biology 2019Quote: ... For “+ATP” conditions 2 mM ATP and an ATP regeneration system (4.5 mM phosphoenolpyruvate, 20 ng/ml pyruvate kinase (Sigma-Aldrich)) were added to the reaction ...
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bioRxiv - Immunology 2021Quote: ... ubiquitin-conjugating enzyme E2 N (UBE2N) inhibitor, Ubc13 inhibitor (Rhyasen et al., 2013) and IκB Kinase inhibitor (PS-1145 dihydrochloride) were purchased from Sigma-Aldrich. LN cells from C57BL6 mice were treated with 10μM of IRAK-Inh ...
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bioRxiv - Molecular Biology 2021Quote: ... and the activity of Rac1 and Cdc42 were assessed by affinity purification using magnetic beads coupled to the p21-binding domain of p21-activated protein kinase (PAK) (Millipore) that specifically binds the active (GTP-bound ...
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bioRxiv - Molecular Biology 2020Quote: ... Single-cell iAT2s were plated on Transwells at a density of 520,000 live cells/cm2 in 100ul of CK+DCI+Y (3uM CHIR99021, 10ng/ml KGF, 50nM dexamethasone, 0.1mM cAMP, 0.1mM IBMX, 10uM Rho-associated kinase inhibitor (Sigma Y-27632)) ...
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bioRxiv - Molecular Biology 2023Quote: ... ADP was produced by incubating radioactive ATP with cold ADP and nucleotide di-phosphate kinase (NDK, N2635-100UN, Sigma-Aldrich) for 30 min at 25°C ...
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bioRxiv - Biophysics 2023Quote: ... 2 ml of cell culture medium containing either 10 μM Rho-kinase (Rock) Inhibitor Y-27632 (Sigma-Aldrich, St. Louis) or identical amounts of PBS (control ...
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The membrane-cytoplasmic linker defines activity of FtsH proteases in Pseudomonas aeruginosa clone CbioRxiv - Biochemistry 2023Quote: ... and 0.1% NP-40) supplemented with 5 mM ATP and an ATP regeneration system consisting of 50 µg/mL creatine kinase (Millapore-Sigma) and 5 mM creatine phosphate (Millipore-Sigma) ...
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bioRxiv - Genetics 2024Quote: ... differentiation of iPSCs was induced through the Wnt modulation method with 7 μM glycogen synthase kinase 3 b (GSK3b) inhibitor CHIR99021 (Selleckchem) and 5 μM inhibitor of WNT production 2 (IWP2) (Sigma). Basal Media was composed of RPMI media supplemented with B27 (Thermo Fisher Scientific) ...
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Synthetase and Hydrolase Specificity Collectively Excludes 2’-Deoxyguanosine from Bacterial AlarmonebioRxiv - Microbiology 2024Quote: ... and ADP production was quantitatively coupled to the stoichiometric consumption of NADH via the activity of excess pyruvate kinase (PK, type-III from rabbit muscle, Sigma) and lactate dehydrogenase (LDH ...
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bioRxiv - Cell Biology 2020Quote: ... and the MEK inhibitor PD0325901 (500 nM; Sigma Aldrich) and withdrawing the p38 MAPK inhibitor SB202190 ...
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bioRxiv - Biochemistry 2021Quote: ... recombinant MEK and recombinant ERK were purchased from Sigma. Indicated amounts of recombinant GST-HSF-1 (0.1–0.4 mg ...
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bioRxiv - Biophysics 2022Quote: ... and an ATP-regeneration system (4 mM ATP, 50 μg/mL creatine kinase [Sigma-Aldrich], 5 mM creatine phosphate [Sigma-Aldrich]). The endpoint fluorescence for complete FITC-casein degradation was determined by addition of porcine elastase (100 μg/mL ...
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bioRxiv - Cell Biology 2022Quote: ... were used as a substrate for phospho-PKCα in a 25 µl in vitro kinase reaction using 100 ng of active recombinant PKCα enzyme (Millipore-Sigma, MA), 5 µl of a lipid activator (Millipore-Sigma ...
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bioRxiv - Physiology 2019Quote: ... All solutions were thawed from stocks at the day of experiments and freshly supplemented with creatine kinase (CK, Sigma/Roche, Germany) to ~300 U/ml or ~3 U/well and sodium azide (0.1 M NaN3) ...
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bioRxiv - Biochemistry 2020Quote: ... RIPK1 kinase was performed on KISSf screening facility (IBISA, Biogenouest, Station Biologique, Roscoff, France) with myelin basic protein (MBP, Sigma, #M1891) as substrate and in the presence of 15µM ATP.
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bioRxiv - Developmental Biology 2022Quote: ... Inhibition of EGFR signaling was performed by injecting 80 μg/g of the kinase inhibitor PD153035 (CAS: 183322-45-4; Sigma-Aldrich), dissolved in DMSO/1xPBS ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: The kinase domain of human HASPIN (residue 452 – 798) was cloned into the expression vector pET-21a(+) (Novagen, Madison, WI, USA). The genomic DNA of HASPIN was provided from the Korea Human Gene Bank ...
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bioRxiv - Immunology 2023Quote: ... were formed by dissociating and maintaining PSCs in ESC media supplemented with 10 μM Rho-associated protein kinase (ROCK) inhibitor (Sigma, Y0503) while being kept on an orbital shaker at 100 rpm for 6 days to allow for spontaneous formation of EBs with hematopoietic potential ...
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bioRxiv - Cancer Biology 2023Quote: Fresh EAC tumor samples were transferred into ice cold conditioned PBS (formula: 10uM rho-associated kinase (ROCK) inhibitor Y27632 (Sigma, #Y0503), 2% penicillin-streptomycin sulfate (Gibco ...
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bioRxiv - Biochemistry 2023Quote: ... The preactivated kinase mixture was then desalted and concentrated using an Amicon™ Ultra-15 30 kDa MWCO spin filter (Millipore) to remove the residual ATP.
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bioRxiv - Biochemistry 2023Quote: ... Serum samples were collected from surviving mice and 3 samples from each group were used for estimation of Creatine Kinase activity Assay Kit (Sigma-Aldrich).
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bioRxiv - Biophysics 2023Quote: ... phosphoenol pyruvate (53 mM PEP, Beantown Chemical, 129745) and pyruvate kinase/lactate dehydrogenase enzymes (2.8% v/v PK/LDH, Sigma, P-0294). For experiments with PRC1 ...
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bioRxiv - Molecular Biology 2023Quote: ... the c-Jun N-terminal kinase inhibitor SP600125 (Cat. S5567) and the c-Myc inhibitor 10058-F4 (Cat. F3680) were from Millipore Sigma.
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bioRxiv - Biochemistry 2023Quote: ... Full-length BTK and BTK linker kinase domain (WT and mutants) were produced by co-expressing with YopH in BL21(DE3) (Millipore Sigma) or BL21-Gold(DE3 ...
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bioRxiv - Biochemistry 2023Quote: GS activities were measured using the pyruvate kinase/ lactate dehydrogenase (PK/LDH) coupled enzymes from rabbit muscle ordered from Sigma-Aldrich (containing 600-1,000 units/ml pyruvate kinase 900-1,400 units/ml lactate dehydrogenase) ...
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bioRxiv - Physiology 2023Quote: ... Analysis of the muscle damage marker creatine kinase (CK) was quantified using spectrophotometry using a coupled enzyme-linked reaction assay (Sigma-Aldrich). Samples were measured in duplicate ...
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bioRxiv - Molecular Biology 2023Quote: ... A stock ATPase reaction mix (20X) contained 20 μL of a mixture of pyruvate kinase and lactic dehydrogenase from rabbit muscle (P0294, Sigma Aldrich), 10 μL of 200 mM NADH (CAS# 606688) ...
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bioRxiv - Plant Biology 2024Quote: ... a similar reaction was performed with both enzymes combined with 10 units phosphoglycerate kinase and 10 units glyceraldehyde-3-phosphate dehydrogenase (both Sigma-Aldrich), alongside 9 mM phospho-enol-pyruvate ...
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bioRxiv - Genetics 2021Quote: ... Medium for CTCF binding site mESCs was further supplemented with 1 mM MEK inhibitor PD0325901 (Sigma) and 3 mM GSK3 inhibitor CHIR99021 (Sigma) ...