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Citations for Millipore Sigma :
3401 - 3450 of 7599 citations for Zika Virus Envelope Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... the proteins were transferred to the polyvinylidene fluoride (PVDF) membrane (0.45 μm, Millipore) and then incubated with GST-14-3-3 ...
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Autophagy suppression in DNA damaged cells occurs through a newly identified p53-proteasome-LC3 axisbioRxiv - Cell Biology 2024Quote: ... and CdtC subunits (within pET-15b protein expression plasmids; #69661, Sigma-Aldrich, USA), each expressed with an amino-terminal polyhistidine fusion peptide ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were detected by immunoblotting with anti-Cdh1 antibodies (1 µg/ml, Millipore) and anti-GST antibodies (0.5 µg/ml ...
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bioRxiv - Microbiology 2024Quote: ... Proteins in the supernatant were precipitated with 1:4 trichloroacetic acid (Sigma Aldrich). The pellet was washed and disrupted with acetone two times and re-dissolved in 200 mM ammonium bicarbonate with 6 M Urea (Sigma Aldrich) ...
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bioRxiv - Biochemistry 2024Quote: ... The eluted protein was concentrated using Amicon Ultra 10K spin columns (Sigma-Aldrich) and dialyzed against hSSB storage buffer.
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bioRxiv - Cancer Biology 2024Quote: ... Lysates were pre-cleared using protein G agarose beads (16-266, Sigma Aldrich). The pre-cleared lysates were incubated with streptavidin-agarose beads (20359 ...
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bioRxiv - Microbiology 2024Quote: ... the proteins were incubated with an equimolar amount of Atto488 maleimide (Sigma-Aldrich) at 4°C overnight ...
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bioRxiv - Cell Biology 2024Quote: ... and FLAG tagged proteins were immunoprecipitated using anti-FLAG M2 affinity gel (Sigma). Elution was carried out with 2X Bolt LDS sample buffer and the samples were blotted onto the PVDF membrane ...
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bioRxiv - Cancer Biology 2024Quote: ... Biotin switch-treated protein reduced with 2.5 μL of 0.2 M dithiothreitol (Sigma) for 1 hr at 57°C and the samples cooled before adding 2.5 μL of 0.5M iodoacetamide and incubated in the dark for 45 minutes ...
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bioRxiv - Developmental Biology 2024Quote: ... FLAG-tagged proteins were pulled down using anti-FLAG M2 magnetic beads (Sigma) and serially diluted with enzyme buffer before ATP and fluorescein-conjugated PKC substrate peptide (ThermoFisher Scientific ...
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bioRxiv - Genetics 2024Quote: ... The eluents were then incubated with S-protein agarose (EMD Millipore, 69704-3) overnight at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: Protein lysates were extracted from flash-frozen pellets with RIPA buffer (Sigma R0278), supplemented with protease and phosphatase inhibitors (Invitrogen A32961) ...
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bioRxiv - Plant Biology 2024Quote: ... Protein and nucleotide concentration were determined using the Bradford reagent (Sigma Aldrich, Germany) and HPLC ...
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bioRxiv - Neuroscience 2024Quote: ... Immobilized proteins were eluted by thrombin cleavage (thrombin 0.02 UN/µl, Sigma, Germany). The unbound fraction (Sbh) ...
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bioRxiv - Molecular Biology 2024Quote: ... the protein was re-applied to His-Select Cobalt affinity gel (Sigma-Aldrich). The flow through fractions were pooled and concentrated (Vivaspin Turbo 3,000 MWCO ...
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bioRxiv - Cell Biology 2024Quote: Proteins were resolved in 10% SDS PAGE and transferred to PVDF membrane (Millipore, Burlington ...
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bioRxiv - Immunology 2024Quote: ... The proteins on the gel were then transferred to PVDF membranes (Millipore, IPVH00010), which were blocked in 5% BSA for 1 h at RT ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were transferred to a 0.45µm Immobilon-P Membrane (Millipore Sigma cat. IPVH00010) in ice-cold Tris-Glycine Buffer with 20% methanol in a transfer tank either overnight at 10V or 1 hour at 50V ...
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bioRxiv - Molecular Biology 2024Quote: ... Successful protein transfer was confirmed by incubating membranes in Ponceau S solution (Sigma) for 5 minutes with rocking and membranes were blocked in 5% milk in TBS buffer containing 0.1% Tween-20 for 1 hour with gentle rocking ...
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bioRxiv - Neuroscience 2024Quote: ... mouse anti-glial fibrillary acidic protein (GFAP Cy3-conjugated Sigma-Aldrich, C9205, RRID:AB_476889), rabbit anti-ionized calcium-binding adapter molecule 1 (Iba1 ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were electrophoretically transferred to an Inmobilon-P polyvinylidene difluoride (PVDF) membrane (Millipore) using the Trans-Blot Turbo (Bio-Rad) ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was concentrated using a 30 K Amicon Ultra-4 concentrator (Millipore) and further purified by gel filtration chromatography on a Superdex 200 Increase 10/300 GL column (Cytiva ...
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bioRxiv - Neuroscience 2024Quote: ... and the plasma membrane protein NaK1 (mouse monoclonal, Millipore, 05-369, 1:2000). Membranes were developed using the ECL Prime Western Blotting System (Amersham Protran ...
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bioRxiv - Neuroscience 2024Quote: ... glial fibrillary acidic protein (GFAP) (1:500; Millipore Sigma; Burlington, MA; Cat#: 345860)] in blocking solution overnight at 4°C ...
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bioRxiv - Bioengineering 2024Quote: ... These constructs were used for protein expression in Rosetta (DE3) competent cells (Novagen) cultured in TB as reported previously11,46,47 ...
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bioRxiv - Biochemistry 2024Quote: ... Protein eluate was further concentrated in a 50 kDa MWCO concentrator (Millipore Sigma). Concentrated protein was loaded onto the Superose 6 Increase 10/300 GL (Cytiva ...
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Braf-mutant Schwann cells divert to a repair phenotype to induce congenital demyelinating neuropathybioRxiv - Developmental Biology 2024Quote: ... Proteins were transferred onto a 0.45 µm PVDF Immobilon-P membrane (Millipore, Merck) using the Pierce Power Blotter (Thermo Fisher) ...
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bioRxiv - Neuroscience 2024Quote: ... Aggregated protein was isolated from the samples using phosphotungstic acid (PTA; Sigma-Aldrich) as described.9 ...
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bioRxiv - Microbiology 2024Quote: ... All proteins were concentrated using a BioMax concentrator (EMD Millipore, Burlington, MA, USA) followed by passage through a 0.2-μm Ultrafree-MC centrifugal filtration device (EMD Millipore ...
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bioRxiv - Microbiology 2024Quote: ... parasitica lysates using the OxyBlot Protein Oxidation Detection kit (Millipore, Billerica, MA, USA). Carbonylated proteins were derivatized with 2,4 Dinitrophenyl hydrazine (DNP ...
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bioRxiv - Microbiology 2024Quote: ... and mouse monoclonal anti-HCoV-OC43 nucleocapsid protein (1:1000, Millipore Sigma, MAB9012). Secondary antibodies coupled to HRP were purchased from molecular probes and Jackson Immuno-research.
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bioRxiv - Neuroscience 2024Quote: ... Protein bands were visualized with the enhanced chemiluminescent HRP substrate Crescendo Immobilon (Millipore). Analysis was performed in ImageJ ...
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bioRxiv - Biophysics 2021Quote: ... Eluted protein was concentrated with Amicon® 3 kDa MWCO concentrator (Millipore, Burlington, MA) and injected into a 10/300 Superdex 75 size-exclusion chromatography (SEC ...
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bioRxiv - Biophysics 2021Quote: ... Protein bands were investigated by using anti-αS monoclonal antibody (Syn 211, Millipore Sigma) and anti-mouse horse radish peroxidase secondary antibody ...
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bioRxiv - Cancer Biology 2021Quote: ... 100 μg of protein was loaded into a 10 kDa centrifugal filter tube (Millipore), washed twice with 200 μl of UA buffer (8 M urea in 0.1 M Tris-HCl ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were resolved using SDS-PAGE and transferred onto Immobilon-FL PVDF membranes (Millipore). Membranes were blocked using Odyssey blocking buffer (Li-Cor ...
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bioRxiv - Cell Biology 2020Quote: ... protein translation was briefly inhibited by treatment with 20 mg/mL cycloheximide (CHX; Sigma) for 8 h ...
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bioRxiv - Cancer Biology 2021Quote: ... and thereafter protein transferred onto 45 µm Polyvinylidene difluoride (PVDF) membrane (IPVH00010, Merck Millipore). Western blotting analysis was performed by using specific primary and secondary antibodies and signals visualized with chemiluminescence ...
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bioRxiv - Systems Biology 2020Quote: ... Particle-bound proteins were transferred to a filter plate (Millipore MultiScreen, 0.22 µm, MSGVN2250) and centrifuged (1000 g ...
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bioRxiv - Developmental Biology 2021Quote: ... 70µg total protein per sample was reduced with tris-2-carboxyethyl phosphine (TCEP, Sigma) for 1 hour at 60°C at a final concentration of 5mM ...
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bioRxiv - Cell Biology 2020Quote: ... protein lysates were resolved by SDS-PAGE and transferred onto a PVDF membrane (Millipore). Membranes were blocked with 2% BSA in TBS tween20 0.1% and incubated in the presence of the primary and then secondary antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... Protein lysates were prepared using radioimmunoprecipitation assay (RIPA) buffer (Sigma Aldrich, St Louis, MO) as per manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2020Quote: ... and nucleoproteins were extracted using a nuclear protein extraction kit (Merck Millipore, Darmstadt, Germany). Nucleoproteins were separated by electrophoresis on 6/12% sodium dodecyl sulfate (SDS ...
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bioRxiv - Cell Biology 2020Quote: ... Separated proteins were transferred to Immobilon-FL polyvinylidene difluoride (PVDF) membranes (Millipore, Cork, Ireland), which were blocked with a 2:1 ratio of Odyssey blocking buffer (LI-COR Biosciences ...
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bioRxiv - Cell Biology 2020Quote: Proteins were separated in polyacrylamide gel and then transferred to polyvinylidene difluoride membranes (Millipore). These membranes were incubated with primary antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... An equal amount of proteins was incubated with anti-HA affinity matrix (Sigma, #11815016001) to pull-down SMO-HA ...
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bioRxiv - Cell Biology 2020Quote: ... Protein expression was induced by 0.2 mM Isopropyl-ß-D-thiogalactopyranoside (IPTG; Sigma-Aldrich) and continued at 20°C for 20 h ...
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bioRxiv - Cell Biology 2020Quote: ... isolated heart mitochondria were solubilized with digitonin (Sigma Aldrich, 6g/g digitonin/protein ratio) and resolved on a 4-12% Bis-Tris polyacrylamide gradient gel (Genscript) ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were eluted by adding 100 µl of 200 µg/ml 3xFLAG peptide (Sigma) in TBS and incubating with rotation at 4°C for 30 min.
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bioRxiv - Genetics 2021Quote: ... antibodies and precipitated proteins were analyzed by western blot using mouse anti-HA (Sigma) or mouse anti-Flag (Sigma) ...