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Citations for Millipore Sigma :
3401 - 3450 of 7143 citations for Borrelia burgdorferi sensu stricto B31 P39 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... and protein complexes were eluted with 150 mg/ml of 3X FLAG peptide (Sigma). Samples were diluted in 2X SDS sample buffer and analyzed with SDS–PAGE according to standard procedures ...
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bioRxiv - Cell Biology 2023Quote: ... the protein was concentrated using a 50 kDa cut-off Amicon filter (Merck Millipore) and loaded onto a pre-equilibrated Superdex 200 Increase 10/300 GL column (Cytiva) ...
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bioRxiv - Neuroscience 2023Quote: ... Equal amounts of proteins were incubated overnight with the anti-FLAG M2 (Sigma M8823) or anti-HA (Pierce #88836 ...
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bioRxiv - Microbiology 2023Quote: ... followed by protein precipitation after the addition of trichloroacetic acid (TCA, Sigma-Aldrich, 91228), (v:v ...
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bioRxiv - Molecular Biology 2023Quote: RIP was performed using a Magnetic RIP RNA-Binding Protein Immunoprecipitation (Millipore, 17-700) by the manufacturer’s guidelines ...
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Dynamics of the epigenetic landscape during development and in response to drought stress in sorghumbioRxiv - Plant Biology 2023Quote: ... H2A.Z (preparation using antigen “GKGLLAAKTTAAKSAEKDKGKKAPV”) were incubated with Protein A agarose (16-157, Millipore) before mixed with sonicated chromatin ...
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bioRxiv - Pathology 2023Quote: Protein was extracted by 1x RIPA lysis buffer (10x, stock solution, EMD Millipore, MA) from mouse stomach tissue ...
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bioRxiv - Genetics 2023Quote: ... 20 μL Magna ChIP™ Protein A+G Magnetic Beads (Sigma-Aldrich 16-663) was washed four times with 1X DPBS supplemented with 5 mg/mL BSA and subsequently crosslinked to 5 μg antibody (Anti-FOXA1 antibody ...
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bioRxiv - Genetics 2023Quote: ... and the supernatants were precleared with 10 μL of Protein A-coated agarose (Millipore). The precleared supernatants were then divided into two equal parts and incubated with 20 μL 14-3-3 polyclonal antibody and IgG overnight at 4 °C ...
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bioRxiv - Genomics 2023Quote: ... the supernatants were collected and protein concentration was measured by Bradford assay (Sigma-Aldrich). Extracts were boiled in presence of SDS loading buffer and analyzed by Western blotting.
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bioRxiv - Molecular Biology 2023Quote: ... separated by SDS-PAGE and proteins transferred to polyvinylidene difluoride membrane (Immobilon-P, Millipore). Membranes were blocked in 5% milk in PBS/ 0.1% Tween-20 ...
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bioRxiv - Molecular Biology 2023Quote: ... the protein concentration was measured using a Bradford assay (Sigma-Aldrich/Millipore B6816-500ML) as per manufacturer’s instructions in optically clear 96-well plate (VWR 62402-933 ...
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bioRxiv - Molecular Biology 2023Quote: ... the protein concentration was measured using a Bradford assay (Sigma-Aldrich/Millipore B6816-500ML) as per manufacturer’s instructions in optically clear 96-well plate (VWR 62402-933 ...
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bioRxiv - Cell Biology 2023Quote: ... the protein was concentrated using a 50 kDa cut-off Amicon filter (Merck Millipore) and loaded onto a pre-equilibrated Superdex 200 Increase 10/300 GL column (Cytiva) ...
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bioRxiv - Cell Biology 2023Quote: ... protein lysates were extracted from BMDMs using Radioimmunoprecipitation assay (RIPA) buffer (Sigma-Aldrich, R0278) complimented with protease inhibitor (Roche Diagnostics ...
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bioRxiv - Neuroscience 2023Quote: ... PLP (Anti-Myelin Proteolipid Protein Antibody, CT, clone PLPC1 MAB388 Sigma-Aldrich, 1:200), MHCII (Dako 1:50) ...
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bioRxiv - Biophysics 2023Quote: ... GST-tagged Elf1 protein was expressed in Escherichia coli strain Rosetta 2(DE3) (Novagen) and purified by Glutathione Sepharose 4 Fast Flow resin (GE Healthcare) ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were resolved in 10% SDS/PAGE and transferred to nitrocellulose membranes (Sigma Aldrich). Membranes were probed for 2 h at RT (or overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP, Sigma-Aldrich) and alkylated with 10 mM chloroacetamide (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... The recombinant proteins were concentrated using an Amicon Ultra (10K; Millipore, Billerica, MA, USA).
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated by SDS-PAGE and transferred to polyvinyl difluoride (PVDF) membranes (Millipore) according to standard protocols ...
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bioRxiv - Developmental Biology 2023Quote: ... supernatant containing protein suspension was collected and quantified with Bradford reagent (Sigma, B6916-500ML) at the spectrophotometer ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... protein levels was done using heparin affinity columns (Sigma-Aldrich, St. Louis, MO, USA) and Western blot analysis as previously described [37 ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were transferred to a polyvinylidene difluoride immobilon TM-P membrane (Millipore, Burlington, MA) overnight at 4°C and blocked in 5% nonfat powdered milk in TBS with Tween 20 (pH 7.6 ...
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bioRxiv - Plant Biology 2024Quote: ... Purified proteins were concentrated using 10 kDa ultra-centrifugal filter Amicon (Merck Millipore, Germany). The quality and quantity of proteins were analyzed by SDS-protein gel electrophoresis (Bio-Rad) ...
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bioRxiv - Microbiology 2024Quote: ... was subjected to affinity purification using S-protein-agarose (Merck Millipore, Burlington, MA, U.S.A.) for 1– 2 h and washed 4 times ...
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bioRxiv - Microbiology 2024Quote: ... purified RSP protein was mixed with Biotin-NHS (Biotinamidocaproate N-hydroxysuccinimide ester, Sigma Aldrich) and three rounds of MACS (with RSP concentrations of 100 nM (the first one ...
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bioRxiv - Biophysics 2024Quote: ... Protein bands were transferred by tank transfer onto a polyvinylidene difluoride (PVDF, EMD Millipore) membrane in transfer buffer (25 mM Tris pH 8.3 ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP, Sigma-Aldrich) and alkylated with 10 mM chloroacetamide (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were reduced with 20 mM DL-Dithiothreitol (DTT; BioUltra, for molecular biology, Sigma) at 56°C for 30 minutes ...
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bioRxiv - Microbiology 2024Quote: ... Protein precipitates were solubilized in 6M urea and subsequently quantified using BCA kit (Sigma). Ten micrograms of the protein samples were reduced with 10mM dithiothreitol (DTT ...
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bioRxiv - Biochemistry 2024Quote: ... 100 mM Tris pH 8.5 with 800 ng of yeast enolase protein (Sigma-Aldrich) added as an internal protein control ...
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bioRxiv - Neuroscience 2024Quote: ... The Syt1 and Syt7 proteins were further treated with Benzonase (Millipore Sigma, Burlington, MA) at room temperature for 1 hr with Syt1 additionally being run through ion exchange (Mono S ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were eluted using 44 μl FLAG-peptides (200 μg/ml; Merck/Sigma Aldrich) in 1x TBS ...
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bioRxiv - Biophysics 2024Quote: ... Protein was initially purified using affinity chromatography using HIS-Select Cobalt Affinity Gel (Sigma) on a gravity flow column ...
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bioRxiv - Biophysics 2024Quote: ... The final concentration of the protein as estimated from the BCA test (Sigma Aldrich) was 8.4 mg/ml.
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bioRxiv - Cell Biology 2024Quote: ... Solubilized protein was added to pre-equilibrated anti-FLAG agarose affinity resin (Sigma, A2220) and incubated for 90min at 4°C on a rotating wheel ...
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bioRxiv - Cell Biology 2024Quote: ... Cleaved proteins in the supernatant were concentrated with a 100K (MWCO) concentrator (EMD Millipore) to 500 μL and purified via SEC on a TSKgel G4000SWXL column (TOSOH Bioscience ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were incubated with 20μl α-V5 or α-FLAG M2 resins (Sigma-Aldrich) for 4 hours at 4°C to pull down CLK or CK1α ...
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bioRxiv - Biochemistry 2024Quote: ... 100 mM Tris pH 8.5 with 800 ng of yeast enolase protein (Sigma-Aldrich) added as an internal protein control ...
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bioRxiv - Bioengineering 2024Quote: ... After dialysis the protein was concentrated through Amicon centrifugal filters (Millipore, Burlington, MA, USA) with a molecular weight cutoff of 3 kDa ...
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bioRxiv - Biophysics 2024Quote: ... Heterologous protein expression was then initiated with 0.02% w/v L-Arabinose (Sigma A3256) overnight at 30 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... proteins were resolved by SDS-PAGE and transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore). Immunoblots were developed using Western Lightning Chemiluminescence Reagent Plus (Advansta ...
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bioRxiv - Physiology 2024Quote: ... The membrane with transferred protein was stained with Ponceau S stain (Sigma-Aldrich #P7170) to check the quality of transfer and protein load ...
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bioRxiv - Molecular Biology 2024Quote: ... The protein bands were visualised by adding Immobilon Forte Western HRP substrate (Millipore #WBLUF0100) on a ChemiDoc XRS+ (BioRad).
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bioRxiv - Biochemistry 2024Quote: ... Protein fractions were collected and concentrated in a 10 kDa MWCO concentrator (Millipore Sigma), flash frozen in liquid nitrogen and stored at −80 °C.
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... a primary antibody against the HCoV-OC43 N protein (Millipore #MAB9013; diluted 1:500) was used ...
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bioRxiv - Immunology 2024Quote: ... Protein expression was induced by adding isopropyl β-D-thiogalactopyranoside (IPTG; 2.0 mM, Sigma). Incubation was continued for another 4h at 37°C in a shaking incubator ...
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bioRxiv - Immunology 2024Quote: ... The first two immunizations included 100µg/100µl OVA protein (Sigma Aldrich, 9006-59-1) in complete Freund’s adjuvant (CFA ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were separated on 5.5% polyacrylamide (PAA) gel and transferred on PVDF membrane (Millipore) using semi-dry blotting for 60 min ...