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Citations for Millipore Sigma :
3301 - 3350 of 10000+ citations for Anti Myc magnetic beads since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... The phosphorylated peptides were released from IMAC beads by 3 × 100 μL 500 mM dibasic sodium phosphate (Na2HPO4, Sigma, S9763), pH 7.0 ...
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bioRxiv - Cancer Biology 2020Quote: ... Beads were washed with TBS-lysis buffer and proteins were eluted from beads by addition of 3×Flag peptides (150 μg/mL, Sigma) for 1 h at 4°C and then subjected to tryptic digestion followed by LC-MS/MS ...
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bioRxiv - Microbiology 2021Quote: ... Lysates were first precleared by incubation with 40 µL of protein A-sepharose beads CL-4B (Sigma, #GE17-0963-03) for 1 h at 4°C and then incubated with mouse mAb anti-HA (clone 16B12 ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 lung titers were quantified by homogenizing harvested lungs in PBS (Quality Biological Inc.) using 1.0 mm glass beads (Sigma Aldrich) and a Beadruptor (Omni International Inc.) ...
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bioRxiv - Biochemistry 2019Quote: ... were incubated overnight at 4°C with the chromatin extracts and then coupled with 50 μl of protein-G-sepharose beads (GE17-0618-01, Sigma) during 4h at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... Cell lysates were centrifuged 20 minutes at 4°C at 45000 g and the supernatants were incubated with glutathione-coupled Sepharose 4B beads (Sigma) for 30 minutes at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were centrifuged at 4°C for 10 min at 20.000xg and supernatant further cleared by incubation with Easy view Protein G agarose beads (Sigma-Aldrich) for 30 min at 4°C (only for FLAG IP) ...
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“A Proteogenomic workflow reveals distinct molecular phenotypes related to breast cancer appearance”bioRxiv - Systems Biology 2020Quote: ... 2 μL of SP3 beads (1:1 ratio of Sera Mag A and Sera Mag B re-suspended in ultrapure water; Sigma) was added to dried peptides and incubated for 2 min under gentle agitation ...
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bioRxiv - Neuroscience 2021Quote: ... NEL-MG were treated with 2 μL of red fluorescent latex beads (2 μm, Sigma-Aldrich, St. Louis, MO, USA), HiLyte™ Fluor 488-labeled amyloid β peptide 25-35 (2 μL) ...
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bioRxiv - Molecular Biology 2020Quote: ... the excess of Picosurf-1 was removed from the nanoreactor bead emulsion and 1H,1H,2H,2H-perfluorooctanol (PFO; Sigma) was added to the same volume ...
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bioRxiv - Genetics 2020Quote: ... Bead-beating was done in an Axygen 1.1 ml plate (P-DW-11-C-S) with 250 μl of acid washed 425–600 μm beads (Sigma G8722) per well ...
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bioRxiv - Genomics 2021Quote: ... at room temperature with a thick layer of self-indicating silica gel (a granular material with c. 3 to 5mm irregular beads; e.g. Type III Sigma-Aldrich, S7625 ...
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bioRxiv - Cell Biology 2019Quote: ... Elution was done by incubation of the beads in 30µl GST elution buffer [50 mM Tris-HCl (pH 8.0) and 20mM reduced glutathione (Sigma-Aldrich, #G4251)] at room temperature for 20mins ...
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bioRxiv - Cell Biology 2019Quote: ... bleach was washed off using HBSS and parasites were added to 4 g sterile glass beads (180 μM; Sigma-Aldrich). Oocysts were vortexed on high speed for 15 s on/15 s off for a total duration of 2 min to disrupt the oocyst wall mechanically ...
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Systematic Analysis of Lysine Succinylation in Vero cells infected with Small Ruminant MorbillivirusbioRxiv - Genomics 2021Quote: ... The bound peptides were eluted 3 times from the beads with 0.1% trifluoroacetic acid (TFA; Sigma-Aldrich, Saint Louis, USA). The eluted fractions were pooled together and vacuum-dried ...
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bioRxiv - Genetics 2020Quote: ... and precipitated with antibodies overnight at 4°C followed by incubation for 2 h with protein G Sepharose beads (Millipore). Normal mouse or rabbit IgG (Santa Cruz ...
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bioRxiv - Immunology 2021Quote: SARS-CoV-2 lung titers were quantified by homogenizing harvested lungs in PBS (Quality Biological Inc.) using 1.0 mm glass beads (Sigma Aldrich) and a Beadruptor (Omni International Inc.) ...
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bioRxiv - Biochemistry 2021Quote: ... “wash III” (500 μl 100 % acetonitrile, and the “on-bead-digestion” plate (100 μl 20 mM Tris pH 8.5, Sigma-Aldrich 10708976001 ...
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bioRxiv - Cell Biology 2021Quote: ... then centrifuged at 10,000 x g for 10 min and the supernatant fraction (800 μl) was applied to 40 μl streptavidin-agarose beads (Sigma) followed by rotation overnight at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... Protein complexes were pulled down by 1.5 h incubation with Protein A agarose beads (Millipore; endogenous RNA pol II IPs) or GFP-Trap®_A beads (Chromotek ...
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bioRxiv - Cell Biology 2020Quote: ... All supernatant was removed using a 1 ml syringe and the beads were resuspended in 30 μl 2X SDS Sample Buffer (Merck Millipore). The samples were incubated at 98°C for 5 minutes before electrophoresis.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Supernatant was then transferred to a 15 mL tube and incubated with 1 mL His bead slurry (Sigma-Aldrich; P6611) for 20 minutes at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... Biotinylated cells were lysed in 1% Triton X-100 containing lysis buffer followed by pulldown with Streptavidin-agarose beads (Sigma) overnight and western blot analysis with indicated antibodies.
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bioRxiv - Immunology 2021Quote: ... with 1% milk and incubated for 2 hours with the mixture of antigen-coupled microsphere beads in 96-well filter bottom plates (Millipore). After washing with assay buffer ...
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bioRxiv - Cell Biology 2019Quote: Approximately 100 OD600 of log-phase cells grown exponentially in YPD were harvested and washed twice with water then lysed by beating with 0.5-mm-diameter glass beads (Sigma-Aldrich) and ice cold ...
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bioRxiv - Cell Biology 2020Quote: ... All supernatant was removed using a 1 ml syringe and the beads were resuspended in 30 μl 2X SDS Sample Buffer (Merck Millipore). The samples were incubated at 98°C for 5 minutes before electrophoresis.
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bioRxiv - Cell Biology 2021Quote: ... placed on silica beads for 5 min for dehydration and then covered with a droplet of halocarbon oil 200 (Sigma) and an oxygen permeable membrane (YSI) ...
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bioRxiv - Microbiology 2021Quote: Samples were transferred to a 2 ml tube containing 200 mg of ≤106 μm glass beads (Sigma, St. Louis, MO) and 0.3 ml of Qiagen ATL buffer (Valencia ...
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bioRxiv - Microbiology 2020Quote: SARS-CoV-2 lung titers were quantified by homogenizing harvested lungs in PBS (Quality Biological Inc.) using 1.0 mm glass beads (Sigma Aldrich) and a Beadruptor (Omini International Inc.) ...
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bioRxiv - Microbiology 2020Quote: ... Whole fish were mechanically disrupted in Lysing Matrix tubes containing 1 ml of sterile water and 425-600 µm glass beads (Sigma). Samples were homogenized at 6.0 m s-1 for 45 s on a FastPrep Cell Disrupter (BIO101/FP120 QBioGene ...
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bioRxiv - Microbiology 2019Quote: ... The bacterial cells were washed with PBS and diluted 1:20 in PBS and then mixed with an equal volume of autoclaved Cytodex-1 beads (131 to 220 μm; Sigma) in PBS ...
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bioRxiv - Immunology 2021Quote: ... or 2 μM NH-3 in the presence of 10 nM T3 for 24 h before the addition of 3 μL of a fluorescent latex bead suspension (L0280, Sigma) in complete DMEM in a ~100:1 bead-to-cell ratio for 2 h before the end of the experiment ...
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bioRxiv - Molecular Biology 2021Quote: ... The lysates were then diluted 10-fold to reduce SDS concentration to 0.1% before immunoprecipitating with Flag antibody conjugated agarose beads (Sigma #A2220). Bead-lysate mixtures were incubated overnight at 4°C using an end-over-end rotator ...
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bioRxiv - Molecular Biology 2021Quote: ... The resuspended polysomes were added to extraction buffer to a final volume of 5 ml with the addition of 100 μl washed FLAG beads (Sigma). Polysomes were incubated with the beads for 2 h with gentle rocking at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: Whole blood was isolated and cleaned from white blood cells and erythrocytes using cellulose-glass bead columns and saponin (0.3%; Sigma-Aldrich) lysis ...
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bioRxiv - Molecular Biology 2020Quote: ... whole blood was cleaned from white blood cells and erythrocytes using cellulose-glass bead columns and saponin (0.3%; Sigma-Aldrich) lysis ...
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bioRxiv - Bioengineering 2021Quote: ... All samples were quantified and agitated overnight at 4°C with 23 μl of Rhotekin RBD agarose bead (EMD Millipore). Equal amounts of protein were subject to SDS-PAGE and blotted with anti-Rho antibody (3 μg/mL ...
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bioRxiv - Genetics 2019Quote: ... The beads were washed 3x with wash buffer and proteins were eluted with FLAG peptide (200 mg/ml, Sigma-Aldrich) in TBS ...
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bioRxiv - Immunology 2022Quote: ... W6/32-bound sepharose beads were then washed with 0.2 M sodium borate pH 9 (#B3545-500G, Sigma-Aldrich, Merck) and fresh 20 mM dimethylpimelimidate (#D8388-250MG ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were placed in a 2.0 mL BeadBug pre-filled microcentrifuge tube on ice containing 1.0 mm acid washed silica beads (Sigma, Burlington, MA) and 1 mL of total lysis buffer solution ...
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bioRxiv - Immunology 2022Quote: ... with 1% milk and incubated for 2 hr with the mixture of antigen-coupled microsphere beads in 96-well filter bottom plates (Millipore). After washing three times with assay buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... 2µL of SP3 beads (1:1 ratio of Sera Mag A and Sera Mag B re-suspended in ultrapure water; Sigma) were added to dried peptides and incubated for 2min under gentle agitation ...
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bioRxiv - Bioengineering 2022Quote: ... the glass coverslip used to localize the beads was removed, and a 0.2% (m/v, in PBS) gelatin (Sigma G1393) coating was conjugated to the polyacrylamide gels using N-ethyl-N’-(3-(dimethylamino)propyl)carbodiimide/N-hydroxysuccinimide (EDC/NHS ...
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bioRxiv - Cell Biology 2022Quote: ... 80-mer oligonucleotides biotinylated either on the 5’ or 3’ end containing the E-box of the Lgr5 promoter or a mutated E-box (see table) were annealed with the corresponding antisense sequence and bound to streptavidin agarose beads (S1638, Millipore). Beads were washed 3 times ...
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bioRxiv - Cell Biology 2022Quote: ... cell lysates were incubated in a rotating incubator for 1 h at 4°C with bead-conjugated FLAG (Sigma; A2220), bead-conjugated HA (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2022Quote: ... then the rest was split into two tubes with addition of 30 μl prewashed Protein A Agarose beads (Sigma, 11134515001), 3 μg anti-RAD51 (PHYTOAB ...
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bioRxiv - Microbiology 2022Quote: ... series of 6 samples were prepared by pipetting 200 μL of inactivated bacterial cell suspension prepared into 1.5 mL Eppendorf tubes filled with approximately 70 μL of 150-212 μm glass beads (Sigma-Aldrich). 50 μL of a trypsin solution (Porcine pancreas grade ...
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bioRxiv - Molecular Biology 2022Quote: ... 30 µl GFP-Trap agarose beads (proteintech) were blocked with 2% (w/v) BSA and 0.1 mg/ml yeast tRNA (Sigma-Aldrich) in lysis buffer for 1 h at 4°C to minimize non-specific interactions ...
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bioRxiv - Immunology 2022Quote: ... resuspended in FACs buffer and acquired within 24 h on a Cytek Aurora flow cytometer following addition of cell counting beads (Sigma).
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bioRxiv - Physiology 2022Quote: ... frozen heart tissues were collected in Precellys® Tissue Homogenizing Mixed Beads Kit (2.0 mL) and homogenized using a PRECELLYS homogenizer (Bertin) containing ice-cold RIPA lysis buffer (R0278, Sigma-Aldrich), supplemented with protease inhibitor mix (cOmplete ...