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Citations for Millipore Sigma :
3551 - 3600 of 10000+ citations for Anti Myc magnetic beads since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... The GST-Cdc14 constructs were expressed at 16°C overnight and the protein purification followed the same procedure as for 6His-3Flag-Cdc14 using glutathione-Sepharose beads (Millipore-Sigma, 17075601) and the following buffers ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were adjusted to 1 mg/ml in lysis buffer and subject to overnight immunoprecipitation at 4°C with either USP7 or USP11 antibody and protein A agarose beads (Sigma-Aldrich), or with GFP-NanoTrap beads (prepared in house ...
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bioRxiv - Microbiology 2020Quote: ... Immunoprecipitation of FLAG-tagged Hfq was performed by incubating each sample with 75 µl pre-washed (3 x resuspended in 1 ml lysis buffer and centrifuged at 10,000 rpm for 1 min) protein A sepharose beads (Merck/Sigma-Aldrich #P6649) for 45 min at 4 °C (rocking) ...
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bioRxiv - Microbiology 2020Quote: ... The washed beads were air dried for ~15 min and then resuspended in 2.5 μLof 2 M betaine (Sigma, ref: B3501).
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bioRxiv - Systems Biology 2020Quote: ... Bait proteins from the supernatant were immuno-precipitated for 90 mins at 4°C with FLAG-M2 agarose beads (Millipore Sigma) and washed three times in the lysis buffer ...
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bioRxiv - Systems Biology 2020Quote: ... The equivalent of one hundred OD600 of lysed cells was used for the immunoprecipitation of the bait proteins using FLAG-M2 agarose beads (Millipore Sigma). The beads were incubated with the cell lysates for 120 mins at 4°C with slow rotation ...
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bioRxiv - Plant Biology 2021Quote: ... 17 µL of FLAG-tagged effector protein extract was added to the beads with 1 µL of 1 mM acetyl-CoA lithium salt (Sigma-Aldrich) and 2 µL of 1 µM IP6 (Sigma-Aldrich) ...
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bioRxiv - Systems Biology 2020Quote: ... beads were recovered from the emulsion by washing with 2 × 30 mL 6 × saline sodium citrate buffer (diluted from Sigma #S6639) buffer in a 5 µm ÜberStrainer (pluriSelect) ...
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bioRxiv - Immunology 2021Quote: ... IgG-free BSA treatment and blocking prior to opsonization was omitted for beads and surfaces opsonized with human IgG (Sigma, I4506). Free active groups were blocked overnight with 0.5M Tris (pH 8.0 ...
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bioRxiv - Microbiology 2019Quote: ... whole-bodies of virus-injected females (DENV and ZIKV-infected) were homogenised with glass beads in Trizol (Sigma-Aldrich, MA, USA) on a Precellys 24 homogeniser (VWR).
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bioRxiv - Genomics 2020Quote: ... Sheared chromatin was diluted using 3X volume of IP dilution buffer and pre-cleared for at least 4hr at 4°C using IgG beads (Sigma A0919). 1% of pre-cleared chromatin was kept as input ...
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bioRxiv - Biophysics 2020Quote: ... 5 μl washed beads were added with 5 μl of 0.5 mg/ml lectin (lectin from Phaseolus vulgaris, Sigma L2646-25MG) and incubated at 4 °C for 1 hour ...
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bioRxiv - Cell Biology 2021Quote: ... 2 mg of the soluble protein fraction were then transferred into fresh tubes and incubated with 30 μL of monoclonal antiHA agarose beads (Sigma, A2095). Samples were incubated for 2 h with end-over-end rotation at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... Then cell lysates were subject to protein immunoprecipitations using indicated beads (For N-Flag-S-protein-tagged, FLAG M2 (Sigma-Aldrich) or S-protein beads (Millipore ...
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bioRxiv - Molecular Biology 2022Quote: ... acetyl-enriched peptides were eluted from the antibody-bead conjugates with 0.1% trifluoroacetic acid in water and were desalted using C-18 zip-tips (Millipore, Billerica, MA). Samples were vacuum dried and re-suspended in 0.2% FA in water.
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bioRxiv - Cancer Biology 2022Quote: ... and the supernatant containing the sheared chromatin was pre-cleared with Protein G plus-Agarose beads (Cat No. IP04-1.5ML, Merck Millipore, Burlington, USA), which were pre-coated with bovine serum albumin (BSA ...
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bioRxiv - Plant Biology 2022Quote: ... The extract was centrifuged at 18,000 g for 10 minutes at 4 °C and the supernatant incubated for 1 hour at 4 °C with continuous agitation with 100 μL of Sepharose beads (Sigma-Aldrich). The extract was then further centrifuged at 500 g for 2 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... Flash frozen pellets were resuspended in 700 µL Trizol with 350 µL acid washed and autoclaved glass beads (425-600 µm, Sigma G8772). The cells were disrupted using a vortex on top speed for 7 cycles of 15 seconds (the samples were chilled on ice for 30 seconds between cycles) ...
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bioRxiv - Biophysics 2022Quote: ... The E protein was cleaved from the EGFP-8xHis fusion by incubation of the beads with 150 units of thrombin (Sigma-Aldrich) overnight at 4 °C ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The samples were then washed twice with 50 μL of IMAC binding/wash buffer and once with 50 μL of 1% FA and eluted thrice from the IMAC beads to the stage tips with 70 μL of 500 mM dibasic sodium phosphate (pH 7.0, Sigma Aldrich, S9763). The stage tips were then washed once with 100 mL 1% FA ...
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bioRxiv - Neuroscience 2021Quote: ... cell lysate was pre-mixed with 100% ethanol at 1:1 volume ratio and was incubated with pre-washed SP3 beads (Millipore Sigma Cat ...
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bioRxiv - Plant Biology 2021Quote: ... Cell walls were broken by three 30-s cycles at 10000 rpm in the presence of micro glass beads (200-400 µm diameter Sigma-Aldrich, Precellys ...
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bioRxiv - Neuroscience 2021Quote: ... Thorlabs) was flipped into the beam path to detect the signal reflected on gold beads (150 nm Gold nanospheres, Sigma Aldrich) on a photomultiplier tube (MD963 ...
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bioRxiv - Microbiology 2021Quote: ... The soluble fraction was then added to the Protein G sepharose beads coupled to either HA 12CA5 mouse monoclonal IgG (Sigma Aldrich) or anti-Ty1 (BB2 ...
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bioRxiv - Biochemistry 2021Quote: ... Methylated mRNA was eluted by free m6A from the beads and purified with the GenElute™ mRNA Miniprep Kit (MRN70, Sigma). One tenth of the fragmented RNA was saved as an input control for standardization ...
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bioRxiv - Bioengineering 2020Quote: ... Beads were washed with 200 µl MACSPlex buffer and the liquid was removed applying vacuum (Sigma-Aldrich, Supelco PlatePrep; −100 mBar). For counterstaining of captured EVs ...
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bioRxiv - Microbiology 2022Quote: ... Bacteria were lysed by bead-beating in the presence of buffer RA-1 (NucleoSpin RNA isolation kit, Mackery-Nagel) and β-mercaptoethanol (Sigma). RNA isolation and purification was continued according to the protocol supplied by the manufacturer (Nucleospin RNA isolation kit ...
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bioRxiv - Genomics 2022Quote: ... The emulsion was solidified at room temperature for 12 hours and beads were removed using 1H,1H,2H,2H-Perfluoro-1-octanol (Sigma Aldrich), washed thrice with Tris–EDTA-Tween buffer (TET ...
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bioRxiv - Cell Biology 2022Quote: ... The beads were washed three times and then incubated with thrombin (for LC3B/GFP-LC3; 10 μL of thrombin [Sigma-Aldrich; T6884] ...
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bioRxiv - Immunology 2019Quote: ... washed larvae were transferred to 2 ml Eppendorf tubes containing ∼0.5 ml of glass beads (Sigma #9268, size: 425-600 μm) in PBS and larvae were vortexed for 2 min as previously described with minor modifications (Petraki et al. ...
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bioRxiv - Developmental Biology 2020Quote: ... the beads were washed 10 times with RIPA buffer then 5 times with 100 mM ammonium hydrogen carbonate (AMBIC) (Sigma-Aldrich) solution to remove salts and detergents ...
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bioRxiv - Synthetic Biology 2019Quote: ... 100 μL of each final culture dilution was applied and spread on to each plate using 12 glass beads (Millipore Sigma).
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bioRxiv - Genomics 2019Quote: ... of lysis buffer (RLT for RNeasy or TRI reagent for Direct-zol) and acid-washed glass beads (425-600 micron, Sigma-Aldrich). RNA was then purified according to each manufacturer’s protocol for yeast ...
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bioRxiv - Biochemistry 2019Quote: ... supplemented with protease inhibitors and loaded onto a 1 mL HiTrap SP Sepharose FF column connected in tandem with 1 mL streptavidin mutein matrix beads (Sigma Aldrich) packed into a Tricorn 5/50 column ...
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bioRxiv - Genetics 2020Quote: ... Protein-DNA complexes were eluted from the beads by adding 200 μL of ice-cold M2 buffer without detergent containing 3xFLAG peptides (Sigma-Aldrich) (final concentration of 150 ng/μL ...
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bioRxiv - Genetics 2020Quote: ... 500 μL of the crude lysate was incubated with 40 μL of 50 % slurry of α-Flag M2 beads (Sigma-Aldrich) for 2 hours ...
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bioRxiv - Cell Biology 2020Quote: ... lysates were mixed with protein A/G agarose beads (Pierce, Waltham, MA, USA) conjugated to antibodies against FLAG-tag (Sigma-Aldrich) or Exo70 (Santa Cruz ...
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bioRxiv - Developmental Biology 2020Quote: ... RNA was extracted from the other 50 μL of beads (80% of volume for GFP-Trap) using TRI Reagent (Sigma, T9424) and then treated with TURBO DNase (Thermo Fisher ...
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bioRxiv - Molecular Biology 2020Quote: ... the beads were washed seven times in 1.5 ml ice-cold NET-2 and protein was eluted form one third of the beads by addition of 100 μl 2xSDS-load buffer followed by incubation for 3 min at 80-90°C whereas RNA was eluted from two thirds of the beads by addition of 1 ml TRI Reagent (Sigma-Aldrich).
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bioRxiv - Genetics 2020Quote: ... Samples were immunoprecipitated with S9.6 antibodies (8 μ antibody/ 5μ DNA) overnight at 4°C followed by incubation for 3 hours with protein G-Sepharose beads (Millipore) and washed sequentially ...
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bioRxiv - Cell Biology 2020Quote: ... The EGFP-FLAG-tagged proteins were eluted from the beads with 0.1 mg/mL 3x FLAG peptide (Sigma-Aldrich, Cat. #F4799) in TBS at 4°C for more than 1 hr ...
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bioRxiv - Physiology 2021Quote: Cartilage was removed from the glenohumeral joint using a scalpel and placed in reinforced Eppendorf tubes containing 500 mg of ceramic beads (Fisher, 15-340-160) and 200 μL of RIPA buffer with protease and phosphatase inhibitors (Sigma, 5892970001), and homogenized by 2 ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were centrifuged for 10 min at 5000Xg and supernatants were diluted 1:1 with PBS (vol/vol) containing protease/phosphatase inhibitors and incubated overnight at +4°C with streptavidin-agarose beads (EMD Millipore). Beads were washed twice with lysis buffer ...
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bioRxiv - Immunology 2021Quote: ... and α-CD28 (1 μg/mL) or beads conjugated with α-CD3ε and α-CD28 in the presence or absence of 200µM apocynin (ED Millipore). This dose of apocynin was used based on dose response curves using increasing concentrations of apocynin (0-300µM ...
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bioRxiv - Biochemistry 2021Quote: ... The proteins were then eluted from the beads with 100 μL of lysis buffer containing 100 μg/mL 3 × FLAG peptide (F4799, Sigma Aldrich), mixed with sample buffer and incubated on ice for 30 min ...
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bioRxiv - Immunology 2020Quote: ... 50 µl of beads and 50 µl of diluted plasma was added to 96-well microtiter plates (MSBVS 1210, Millipore, USA) pre-wetted with ABE ...
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bioRxiv - Microbiology 2020Quote: ... Biofilms were removed from coverslips by sonication at 35 kHz for 10 minutes in a sonic bath in 1 ml of PBS and the sonicate transferred to a 2.0 ml RNase-free bead beating tube (Sigma-Aldrich, UK). Cells were homogenized in TRIzol ™ (Invitrogen ...
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bioRxiv - Biophysics 2022Quote: ... beads with 2 μm diameter (Kisker PC-S-2.0) were functionalized with Concanavalin A (ConA, from Canavalia, Sigma-Aldrich, C2272-10MG) as follows.
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bioRxiv - Cancer Biology 2022Quote: ... purified the protein using Ni- resin and exchanged the bound nucleotide for non-hydrolysable GTP analogue GMPPCP using alkaline phosphatase beads (Sigma-Aldrich) and following the protocol of John et.al ...
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bioRxiv - Cell Biology 2022Quote: RNA was isolated by phenol chloroform extraction and alcohol precipitation followed by two consecutive rounds of poly-A selection using oligo(dT) beads (GenElute mRNA Miniprep Kit, Sigma MRN10) following the manufacturer’s recommendations ...