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Citations for Millipore Sigma :
3251 - 3300 of 10000+ citations for Human Dual Specificity Phosphatase 3 DUSP3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2021Quote: ... 1% Triton X-100 and 10% glycerol with freshly added protease and phosphatase inhibitor cocktail (Sigma). The protein concentration was determined by Bradford protein assay (Bio-Rad) ...
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bioRxiv - Neuroscience 2020Quote: ... 1.0% Nonidet P-40) with protease and phosphatase inhibitor cocktails (Sigma-Aldrich, St. Louis, MO, USA) was used for the total protein fraction ...
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bioRxiv - Biochemistry 2021Quote: ... EDTA free protease inhibitor cocktail and PhosSTOP phosphatase inhibitor cocktail (Millipore Sigma cat. # 11836170001 and 4906845001). For tissue preparation ...
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bioRxiv - Immunology 2020Quote: ... bound antibodies were detected by addition of alkaline phosphatase-conjugated goat anti-mouse IgG (Sigma-Aldrich), diluted 1/5000 in casein ...
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bioRxiv - Microbiology 2021Quote: ... An alkaline phosphatase-conjugated goat anti-rabbit immunoglobulin G secondary antibody (Sigma-Aldrich, St Louis, MO) at 1:10,000 dilution was added and incubated at room temperature for 1 h ...
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bioRxiv - Immunology 2020Quote: ... the plates were washed and developed using alkaline phosphatase substrate pNPP tablets (Sigma cat. #S0942-200TAB) dissolved in a stain buffer ...
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bioRxiv - Cell Biology 2021Quote: ... following instructions from the manufacturer and using protease and phosphatase inhibitors (Millipore, 524624, 524625, and 539131). Lysates were separated by SDS-PAGE ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by the addition of 1M NH4HCO3 (2 mL) and 1 U of alkaline phosphatase (Sigma). The solution was incubated at 37°C for 2 hours ...
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bioRxiv - Neuroscience 2022Quote: ... incubated for one hour with 1μg/mL goat anti-mouse alkaline phosphatase (Millipore Sigma Cat #AP124A), washed again three times in TBSt and signal was developed with p-nitrophenyl phosphate (pNPP Sigma Cat #N2765) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Triton-X100 0.1%) freshly supplemented with a cocktail of proteases and phosphatases inhibitors (Sigma-Aldrich, #PPC2020), 72h after siRNA transfection ...
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bioRxiv - Neuroscience 2022Quote: ... pH 8.0) containing 1 mM PMSF and protease and phosphatase inhibitor cocktails (Sigma, # 4906845001 and #11836170001) with three 1.4 mm Zirconium oxide beads (Bertin Instruments ...
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bioRxiv - Molecular Biology 2022Quote: Cells were lysed with 100–300 μL RIPA buffer supplemented with protease and phosphatase inhibitors (Millipore). The protein concentration was measured with the BCA Protein Assay (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: ... containing 5% beta-mercaptoethanol and protease and phosphatase inhibitors (Roche Complete Mini protease inhibitor, Sigma-Aldrich #11836153001 ...
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bioRxiv - Cancer Biology 2024Quote: ... 1% protease inhibitor cocktail set III and 1% phosphatase inhibitor cocktail set IV (EMD Millipore Corporation). Cells were scraped ...
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bioRxiv - Immunology 2024Quote: ... 0.1% sodium dodecyl sulfate (SDS)) supplemented with protease inhibitor cocktail and phosphatase inhibitor cocktail (Sigma-Aldrich). Protein concentration was detected by BCA assay (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2023Quote: ... The Alkaline Phosphatase assay was carried out according to the manufacturer’s instructions (Sigma-Aldrich, 86R-1KT). Alkaline phosphatase activity is highest in stem cells ...
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bioRxiv - Immunology 2023Quote: ... 0.1 % Tween20 and incubated with 100 μl of goat anti-mouse IgG alkaline phosphatase conjugate (Sigma) (1/5000 dilution in PBS-0.5 mM EDTA ...
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bioRxiv - Developmental Biology 2023Quote: ... The probe was detected with an anti-digoxigenin antibody conjugated to alkaline phosphatase (11093274910, Sigma-Aldrich). Images were captured using the Zeiss AxioCam HRc camera with the AxioVision software ...
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bioRxiv - Cancer Biology 2023Quote: ... antibodies followed by an anti-mouse secondary antibody conjugated to alkaline phosphatase (AP) (A3562, SIGMA-Aldrich). We developed the immunoblots with BCIP/NBT color development substrate and normalized protein levels to Ponceau Red staining ...
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bioRxiv - Immunology 2023Quote: ... and bound Igs were detected with an antimouse IgM antibody conjugated to alkaline phosphatase (Sigma; A9688), the biotinylated forms of anti-mouse IgG1 (BD Biosciences ...
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bioRxiv - Biophysics 2023Quote: ... Alkaline phosphatase activity was determined by the hydrolysis of p-nitrophenyl phosphate (p-NPP, Sigma-Aldrich) in a reaction medium containing 10 mM p-NPP and 1 mM MgCl2 in AMPOL buffer (2-amino-2-methyl-1-propanol) ...
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bioRxiv - Plant Biology 2023Quote: ... and anti-rabbit secondary antibodies coupled with alkaline phosphatase or horseradish peroxidase were purchased from Sigma. The GFP and mCherry polyclonal antibodies were purchased from Bioeasytech (Beijing).
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bioRxiv - Cancer Biology 2023Quote: Freshly harvested cells were supplemented with phosphatase and protease inhibitor cocktail (#04906837001 and #04693132001, Sigma-Aldrich) and lysed using RIPA lysis buffer (#20-188 ...
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bioRxiv - Neuroscience 2024Quote: ... at 10% w/v with phosphatase and protease inhibitor cocktails (Sigma Aldrich, P5726 and P8340, respectively), and then sonicated on ice for 30 s ...
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bioRxiv - Cell Biology 2024Quote: ... 1% (v/v) NP-40) supplemented with protease cocktail inhibitor and PhoSTOPTM phosphatase inhibitor (Sigma-Aldrich). The cell lysate was centrifuged at 16600 x g for 20 minutes at 4°C and the supernatant was collected for western blotting ...
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bioRxiv - Plant Biology 2024Quote: Extraction buffer (100 mM HEPES pH7.5, 5 mM DTT, MS SAFE Protease and Phosphatase Inhibitor (Sigma)) and leaf powder were mixed in a 1:1 ratio (i.e ...
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bioRxiv - Physiology 2024Quote: ... Activity of alkaline phosphatase was detected in the presence of 1.35% p-nitrophenyl phosphate (Sigma Aldrich) in 0.1 M TRIS buffer pH 8.0 and 0.01 M MgCl2 (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... 150 mM NaCl) supplemented 1% phosphatase inhibitors and 1% protease inhibitors (Sigma-Aldrich; P8340 and P5726). Insoluble debris was removed via centrifugation at 21,000 x g for 10 minutes at 4°C ...
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bioRxiv - Systems Biology 2021Quote: ... purified LC and naïve CD4+ T cells or TRMs were co-cultured in human serum supplemented media (RPMI, Gibco, UK, 10% human serum, Sigma, UK ...
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bioRxiv - Cancer Biology 2020Quote: Human neutrophils were obtained from fresh human peripheral blood from healthy volunteers and were isolated using a Percoll (Sigma-Aldrich) gradient.
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bioRxiv - Microbiology 2020Quote: ... Membranes were probed with polyclonal goat anti-human ACE2 (1:500, AF933, R&D Biosystems) or rabbit anti-human beat-Actin (1:2500, 6L12, Sigma) followed by donkey anti-goat IRdye 680CW or goat anti-rabbit IRdye 800CW (Abcam) ...
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bioRxiv - Physiology 2020Quote: ... For insulin clearance during an insulin challenge, mice were given human insulin (1 U/kg, NovoRapid) or human C-peptide (50 µg/kg, Sigma) by intraperitoneal injection and blood samples were collected by tail vein sampling at 0 ...
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bioRxiv - Microbiology 2020Quote: ... membranes were probed with 1 in 10,000 dilution of human anti-SIC followed by 1 in 50,000 dilution of HRP-conjugated goat anti-human IgG (Sigma-Aldrich). To directly compare detection of SIC fragments by purified human and rabbit anti-SIC antibodies ...
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bioRxiv - Cell Biology 2022Quote: The human alveolar carcinoma epithelial cell line A549 (ATCC, CCL-185) and human colorectal adenocarcinoma cell line HT29 (Sigma Aldrich) were utilized for toxicological evaluation ...
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bioRxiv - Microbiology 2020Quote: ... 15 µl of secondary antibody (anti human Fab-HRP: Jackson ImmunoResearch, cat# 109-035-097; anti human IgG-HRP: Sigma, cat# A6029-1ML ...
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bioRxiv - Immunology 2022Quote: ... alpha enolase (in-house expressed human) and collagen II (bovine purified, chondrex) and at 0.5 μg/ml hIgG1 to citrullinated fibrinogen (human purifed, Sigma Aldrich).
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bioRxiv - Microbiology 2023Quote: Primary human macrophages (MΦs) were cultured in macrophage medium (RPMI-1640 GlutaMAX™ (Thermo) supplemented with 4% human AB serum (Sigma), 100 μg/ml penicillin-streptomycin (Sigma) ...
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bioRxiv - Systems Biology 2024Quote: ... The cells were then incubated with 1ug/ul primary antibody (For TRUB1 staining, TRUB1 Rabbit anti-human polyclonal, 50-172-8037, Protein tech; for PUS7 staining, PUS7 Rabbit anti-human, HPA024116, Sigma) in 1% BSA/PBS-Triton (0.1% ...
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bioRxiv - Immunology 2021Quote: ... Cy3-STxB was generated by conjugating Cy3 dye onto STxB according to the manufacture manual of CY®3 Ab Kit (PA330000, Sigma-Aldrich).
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bioRxiv - Cancer Biology 2022Quote: For RNA-binding protein immunoprecipitation (RIP) ~3×107 A549 cells for each sample were processed using Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore Sigma) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transfected with a 1:3 molar ratio of heavy chain and light chain expression vectors using the Expi293 Transfection kit (Sigma-Aldrich; L3287) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... the cells were lysed and the ATP content was measured by an ATP assay kit per manufacturer’s instruction (Millipore Sigma, n = 3).
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bioRxiv - Cell Biology 2020Quote: ... in serum was analyzed by measuring 3-hydroxybutyric acid (BOH) and acetoacetic acid (AcAc) using Ketone body assay kit (Sigma, No. MAK134) according to manufacturer’s instruction ...
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bioRxiv - Microbiology 2019Quote: ... The a-P28 antibody was conjugated with the Cy3 fluorescent dye using the Cy®3 Ab Kit GE Healthcare (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: The G3P concentration was quantified using the Glycerol-3-Phosphate Assay Kit (MAK207) according to the technical bulletin (Sigma-Aldrich, United States). The concentration of G3P was expressed in ng/μg protein.
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bioRxiv - Cancer Biology 2021Quote: ATP levels were measured in control and constricted breast cancer cells (3 x 105 cells) using the ATP fluorometric assay kit (Sigma-Aldrich, MAK190), following the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 3 and 7 post-drug or vehicle administration were analyzed to quantify urea levels with a urea assay kit (Sigma-Aldrich, MAK006) according to vendor-provided protocol ...
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ADEVO: Proof-of-concept of Adenovirus Directed EVOlution by random peptide display on the fiber knobbioRxiv - Bioengineering 2023Quote: ... viability measurements were performed for the 1 vpc and 3 vpc conditions of all vectors and two wells of uninfected cells with the Cell Counting Kit 8 (CCK8, Sigma-Aldrich #96992). Subsequently ...
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bioRxiv - Cell Biology 2020Quote: ... 40 mL of 3% polyvinyl alcohol (3% w/v PVA) (Sigma-Aldrich CO., St. Louis, MO, USA) were added and the mixture was mechanically stirred at 600 rpm for 4 h (RW-20 ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were then placed in 0.025% 3-3’-diaminobenzidine tetrahydrochloride (DAB, Sigma-Aldrich, St. Louis, MO), 0.01M Imidazole (Fisher Scientific ...