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Citations for Millipore Sigma :
3201 - 3250 of 4915 citations for Recombinant Human CTLA4 Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Zoology 2020Quote: ... Each lysate was confirmed to express the appropriate recombinant protein at the expected size using an anti-V5 antibody produced in rabbit (Sigma V8137). Isoform specificity was tested via immunoblotting all cell lysates (empty vector control ...
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bioRxiv - Plant Biology 2020Quote: ... The recombinant MBP fusion protein was concentrated with an Amicon Ultra-15 centrifugal filter unit (Ultracel 3K; Merck Millipore, Darmstadt, Germany) and used directly for electrophoretic mobility shift assay (EMSA).
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bioRxiv - Cell Biology 2020Quote: ... Base media for the culture of mESCs were supplemented with 1000 units/mL ESGRO Recombinant mouse LIF protein (EMD Millipore ESG1107), and 2i small molecule inhibitors ...
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bioRxiv - Neuroscience 2021Quote: ... αSyn oligomers were produced by incubating recombinant αSyn to a final concentration of 20 µM with 300 µM DOPAL or dopamine (Sigma-Aldrich) in PBS ...
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bioRxiv - Cell Biology 2021Quote: ... 5 mg of protein from each sample was incubated for two hours with purified Multidsk2 recombinant protein bound to glutathione agarose magnetic beads (Sigma G0924). After incubation beads were washed four times with D-buffer and resuspended in NuPage LDS sample buffer (ThermoFisher ...
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bioRxiv - Immunology 2020Quote: ... The bacterial culture was then induced to produce the recombinant protein by the addition of 0.5 M isopropyl β-d-1-thiogalactopyranoside (IPTG) (Sigma-Aldrich) and was allowed to grow for a further 3 h ...
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bioRxiv - Immunology 2021Quote: ... and 2 ml Flt-3L-containing supernatant [produced from SP2/0 transfected cell line secreting mouse recombinant Flt-3L42 for 7 days at 37°C until activation with 100 ng/mL LPS (Sigma-Aldrich) 18 hours prior to the assays.
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bioRxiv - Cell Biology 2021Quote: ... 5a), recombinant mEGFP(−7e) or scGFP(+7e) were dissolved in injection buffer (50 mM K-HEPES, pH 7.4 (Sigma-Aldrich, H3375), 25% glycerol (Applichem ...
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bioRxiv - Microbiology 2022Quote: ... The LDH assay was carried out in 96 or 384 well plate format and a recombinant porcine LDH enzyme (Sigma-Aldrich) was used in each LDH assay plate in a serial dilution to extrapolate a standard curve which was used to estimate LDH released on infection with A ...
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bioRxiv - Neuroscience 2024Quote: ... lateral and medial) were each injected with 10 μl of either PBS or recombinant mouse GDNF (10 μg/ml, Sigma-Aldrich) using 31-gauge insulin syringes without damaging any innervating nerves ...
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bioRxiv - Biochemistry 2024Quote: ... were mixed with 60 ng of recombinant HA-BACH1 in 21 μl of a reaction buffer containing 10 μg of bovine ubiquitin (Sigma-Aldrich), 2 mM ATP ...
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bioRxiv - Synthetic Biology 2023Quote: ... cells were seeded on 6-well cell culture plate wells at a density of ∼2 x 105 cells/well with 2 mL of the culture medium containing 103 units/mL ESGRO Recombinant Mouse Leukemia inhibitory factor (LIF) (Millipore #ESG1107) and 2i (1.0 µM PD0325901 Toris #4423 and 3.0 µM CHIR99021 Wako #038-23101) ...
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bioRxiv - Cancer Biology 2022Quote: ... 7μL of each dilution was then used in SDS-PAGE alongside a dilution series of recombinant GST protein (Sigma-Aldrich #SRP5348) from 5ng-200ng ...
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bioRxiv - Microbiology 2023Quote: ... mixture of BEI-EMCV strain SING-M105 or recombinant VP1 protein prepared according or BEI-EMCV with Complete Freund’s adjuvant (Sigma-Aldrich, USA) was injected intramuscularly into five BALB/c mice at the concentration of 50 μg of antigen per mouse ...
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bioRxiv - Biochemistry 2023Quote: ... Recombinant protein expression was induced with 1 mM Isopropyl β-D-1-thiogalactopyranoside (IPTG, Sigma; 16°C, 200 RPM, 20 hours). Cells were harvested by centrifugation (5000 RPM ...
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bioRxiv - Bioengineering 2023Quote: ... The hybridized RNAs were then mixed with 25 µL of 30 µM (5 mg/mL) recombinant SpCas9 protein (Sigma-Aldrich #Cas9PROT) for 30 minutes at 4 C ...
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bioRxiv - Cancer Biology 2023Quote: ... stable PDA cell lines were generated by infecting 70% confluent cultures with the recombinant lentiviral vectors with 10 mg/mL polybrene (TR-1003, Sigma-Aldrich) twice over 2 consecutive days ...
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bioRxiv - Neuroscience 2020Quote: ... The cell pellet obtained after Percoll layering was plated in non-precoated culture ware for adherent cells and expanded for 4 days in DMEM/F-12 media with 10% FCS (Sigma, St Louis, MO). Microglia were isolated using anti-Cd11b beads (130-049-601 ...
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bioRxiv - Cancer Biology 2019Quote: ... Gartner’s medium consisted of alpha-MEM (BioWhittaker) supplemented with 12.5% heat-inactivated FCS and 12.5% heat-inactivated Horse serum (Sigma-Aldrich, Saint Louis, MO, USA), 1 μM hydrocortisone (Sigma-Aldrich) ...
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bioRxiv - Immunology 2022Quote: ... dispersed cells were cultured for 4 h in RPMI/10% fetal calf serum (FCS) with 1.5 ng/ml phorbol 12-myristate 13-acetate (PMA) and 1 μg/ml ionomycin (all from Sigma-Aldrich, St Louis, Mo) with Golgi-Stop (BD Bioscience ...
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bioRxiv - Neuroscience 2022Quote: ... 1 hemisphere of each mouse brain without cerebellum and olfactory bulbs was placed in FACS buffer (1x DPBS, 2% FCS and 2 mM EDTA) + 5 μM Actinomycin D (ActD, Sigma, Cat#A1410-5MG) for transcriptomics ...
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bioRxiv - Immunology 2023Quote: ... Antibody binding to N protein was detected using conventional two-step ELISA with an HRPO-conjugated anti-mouse or anti-rat Fc antibody (Sigma Aldrich cat # A9309). All assays included a standard curve of mouse neutralising S1 antibody and mouse anti-N antibody (Sino Biological) ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were cultured in an incubator at 37°C and 5% CO2 with 100% humidity in a 1:1 mixture of EGM-2 with M199 (Thermo Fischer, 31150022) supplemented with 10% fetal calf serum (FCS; Sigma-Aldrich, F7524-500ML), 20 µg/ml gentamicin (Thermo Fischer ...
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bioRxiv - Cancer Biology 2021Quote: ... Human primary fibroblasts (HFFF2) were treated with 50μM of Etoposide (Sigma-Aldrich) for 2 days and cells collected at day 7 or treated with 1μM Palbociclib for 7 days ...
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bioRxiv - Cancer Biology 2021Quote: ... Plasma samples were depleted once each using human IgY14 LC10 (Sigma S5074) and human Supermix LC5 (Sigma S5324 ...
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bioRxiv - Cell Biology 2020Quote: All the substrates were functionalized with human plasma fibronectin (FN, Sigma-Aldrich). After sterilizing with UV for 30 min ...
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bioRxiv - Immunology 2021Quote: ... and 10% human serum (heat-inactivated, sterile-filtered, male AB plasma; SIGMA). Human IL-2 (TECIN™ teceleukin ...
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bioRxiv - Molecular Biology 2021Quote: Human islets were washed in 1x PBS and resuspended in Accutase (Sigma) dissociation solution for 3 minutes at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were incubated with rabbit anti-human SOX9 primary antibody (AB5535, Millipore) in the blocking solution for 30 min at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... for Mouse 448 and human experiments or 1 mg/ml Hoechst (Sigma) for the mouse atlas experiments ...
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bioRxiv - Biophysics 2022Quote: ... consisting of OF-medium complemented with 10μg/ml human transferrin (Sigma-Aldrich), 20nM human insulin (Sigma-Aldrich) ...
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bioRxiv - Immunology 2022Quote: ... Macrophage colony-stimulating factor (M-CSF, human) was purchased from Millipore Sigma, St ...
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bioRxiv - Immunology 2022Quote: Human dermal fibroblasts at passage 20 were cultured in complete DMEM (Sigma) supplemented with 1% pen/step (Sigma ...
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bioRxiv - Genomics 2020Quote: ... Purified rabbit polyclonal antibody against human TCF21 (HPA013189) was purchased from Sigma. Recombinant human TGFβ (AF-100-21C ...
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bioRxiv - Cell Biology 2019Quote: ... followed by 7.5 IU human chorionic gonadotropin (Cat.# CG5-1VL, Sigma-Aldrich) 46-48 h later ...
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bioRxiv - Molecular Biology 2020Quote: ... 40 ng human gDNA from buffy coat (Sigma Aldrich, St. Louis, MO) was added in all experiments except those using clinical isolates ...
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bioRxiv - Immunology 2021Quote: ... using the Non-Human Primate Cytokine MILLIPLEX map 23-plex kit (Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... 5% CO2 for 1 hour in human tubular fluid (HTF, EMD Millipore) at 2.0 × 106 cells/ml and co-incubated with oocytes at 2.0 × 105 sperm/ml ...
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bioRxiv - Bioengineering 2021Quote: ... and 5 ng/mL luteinizing hormone from human pituitary (LH, Sigma, L6420), with media changes every other day ...
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bioRxiv - Bioengineering 2021Quote: ... and 5 ng/mL luteinizing hormone from human pituitary (LH, Sigma, L6420), with media changes every other day ...
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bioRxiv - Bioengineering 2021Quote: ... A 100 nM CGRP (human α-CGRP, Sigma-Aldrich, St. Louis, MO) solution was prepared in aCSF ...
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bioRxiv - Bioengineering 2021Quote: ... and human plasminogen (SRP6518) were purchased from Sigma-Aldrich (St. Louis, MO). Ethyl acetate (142-89-2) ...
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bioRxiv - Bioengineering 2020Quote: ... and 15 μl/ml human albumin (Sigma-Aldrich, St. Louis, MO, USA) in Dulbecco’s Phosphate Buffered Saline (DPBS ...
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bioRxiv - Biophysics 2021Quote: ... human ovarian cancer cell line A2780 was purchased from Sigma Aldrich (#93112519), whereas THP-1 monocyte cell line was supplied by a third part ...
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bioRxiv - Cell Biology 2021Quote: ... Insulin solution human was obtained from Sigma (Sigma Aldrich, Dorset, UK, I9278).
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bioRxiv - Microbiology 2020Quote: ... blots were reprobed with mouse anti-human tubulin (1:3000, Sigma T5168). SUMO1 smears above 90 kDa (excluding therefore the high intensity RanGAP1 bands present at 80 kDa ...
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bioRxiv - Microbiology 2020Quote: ... 10ng/ml human KGF (novoprotein, CM88) and 20 µM DAPT (Sigma, D5942) from day 14 to day 21 ...
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bioRxiv - Systems Biology 2021Quote: ... were coated with 12.5 μg/ml human fibronectin (Sigma Aldrich, Darmstadt, Germany) for 1 h at RT ...
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bioRxiv - Immunology 2020Quote: ... were coated overnight at 4°C with anti-human Fab (Millipore Sigma) diluted 1:500 in PBS ...
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bioRxiv - Microbiology 2019Quote: ... Purified mouse anti-human integrin αvβ3 monoclonal antibody (MAB1976) was from Millipore. PE-conjugated goat anti-mouse pAb and alexa 647-conjugated goat anti-rabbit pAb were from Invitrogen ...